{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T03:51:29Z","timestamp":1789876289844,"version":"4.0.1"},"reference-count":25,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2021,9,29]],"date-time":"2021-09-29T00:00:00Z","timestamp":1632873600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>We have assessed the spectral transmittance of the different layers of the human cornea in the ultraviolet (UV), visible, and near-infrared (IR) spectral ranges. Seventy-four corneal sample donors were included in the study. Firstly, the corneal transmittance was measured using a spectrophotometer. Then, all samples were fixed for histopathological analysis, which allowed us to measure the thickness of each corneal layer. Finally, the absorption coefficients of the corneal layers were computed by a linear model reproducing total transmittance. The results show that corneal transmission was almost in unity at the visible and IR ranges but not at the UV range, in which the layer with higher transmission is Descemet\u2019s membrane, whereas the stroma showed the lowest transmittance. Regarding the absorption coefficient, the most absorptive tissue was Bowman\u2019s layer, followed by the endothelium. Variations on transmittance due to changes in the stroma, Bowman\u2019s layer, or Descemet layer were simulated, and important transmission increases were found due to stroma and Bowman changes. To conclude, we have developed a method to measure the transmittance and thickness for each corneal layer. All corneal layers absorb UV light to a greater or lesser extent. The absorption coefficient is higher for Bowman\u2019s layer, while the stroma is the layer with the lowest transmittance due to its thickness. Variations in stroma thickness or changes in the corneal tissue of Bowman\u2019s layer or the endothelium layer due to some pathologies or surgeries could affect, to a greater or lesser degree, the total transmission of the cornea. Thus, obtaining accurate absorption coefficients for different layers would help us to predict and compensate these changes.<\/jats:p>","DOI":"10.3390\/jcm10194490","type":"journal-article","created":{"date-parts":[[2021,9,29]],"date-time":"2021-09-29T08:27:44Z","timestamp":1632904064000},"page":"4490","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Spectral Transmission of the Human Corneal Layers"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5992-5006","authenticated-orcid":false,"given":"Cristina","family":"Peris-Mart\u00ednez","sequence":"first","affiliation":[{"name":"Anterior Segment and Cornea and External Eye Diseases Unit, FISABIO-Oftalmolog\u00eda M\u00e9dica (FOM), Av. P\u00edo Baroja, 12, E-46015 Valencia, Spain"},{"name":"Surgery Department, Ophthalmology, School of Medicine, University of Valencia, Av. Blasco Ib\u00e1\u00f1ez, 15, E-46010 Valencia, Spain"},{"name":"Department of Optics and Optometry and Vision Sciences, Physics School, University of Valencia, Dr. Moliner, 50, E-46100 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8736-0214","authenticated-orcid":false,"given":"Mari Carmen","family":"Garc\u00eda-Domene","sequence":"additional","affiliation":[{"name":"Anterior Segment and Cornea and External Eye Diseases Unit, FISABIO-Oftalmolog\u00eda M\u00e9dica (FOM), Av. P\u00edo Baroja, 12, E-46015 Valencia, Spain"},{"name":"Department of Optics and Optometry and Vision Sciences, Physics School, University of Valencia, Dr. Moliner, 50, E-46100 Valencia, Spain"},{"name":"Thematic Cooperative Health Network for Research in Ophthalmology (Oftared), Carlos III Health Institute, C\/Sinesio Delgado, 4, E-28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariola","family":"Penad\u00e9s","sequence":"additional","affiliation":[{"name":"Anterior Segment and Cornea and External Eye Diseases Unit, FISABIO-Oftalmolog\u00eda M\u00e9dica (FOM), Av. P\u00edo Baroja, 12, E-46015 Valencia, Spain"},{"name":"Thematic Cooperative Health Network for Research in Ophthalmology (Oftared), Carlos III Health Institute, C\/Sinesio Delgado, 4, E-28029 Madrid, Spain"},{"name":"Pathology Group, PASAPTA, Veterinary School, Cardenal Herrera-CEU University, C\/Tirant lo Blanc, 7, E-46115, Alfara del Patriarca, E-46115 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5918-8429","authenticated-orcid":false,"given":"Mar\u00eda Josefa","family":"Luque","sequence":"additional","affiliation":[{"name":"Anterior Segment and Cornea and External Eye Diseases Unit, FISABIO-Oftalmolog\u00eda M\u00e9dica (FOM), Av. P\u00edo Baroja, 12, E-46015 Valencia, Spain"},{"name":"Department of Optics and Optometry and Vision Sciences, Physics School, University of Valencia, Dr. Moliner, 50, E-46100 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ester","family":"Fern\u00e1ndez-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Anterior Segment and Cornea and External Eye Diseases Unit, FISABIO-Oftalmolog\u00eda M\u00e9dica (FOM), Av. P\u00edo Baroja, 12, E-46015 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Mar\u00eda","family":"Artigas","sequence":"additional","affiliation":[{"name":"Anterior Segment and Cornea and External Eye Diseases Unit, FISABIO-Oftalmolog\u00eda M\u00e9dica (FOM), Av. P\u00edo Baroja, 12, E-46015 Valencia, Spain"},{"name":"Department of Optics and Optometry and Vision Sciences, Physics School, University of Valencia, Dr. Moliner, 50, E-46100 Valencia, Spain"},{"name":"Thematic Cooperative Health Network for Research in Ophthalmology (Oftared), Carlos III Health Institute, C\/Sinesio Delgado, 4, E-28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,29]]},"reference":[{"key":"ref_1","first-page":"776","article-title":"Transmission of the ocular media","volume":"1","author":"Boettner","year":"1962","journal-title":"Investig. Ophthalmol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1007\/BF01204614","article-title":"Spectral transmission of the optical media of the human eye with respect to keratitis and cataract formation","volume":"88","author":"Ambach","year":"1994","journal-title":"Doc. 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