{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T22:52:44Z","timestamp":1774047164374,"version":"3.50.1"},"reference-count":85,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2021,12,27]],"date-time":"2021-12-27T00:00:00Z","timestamp":1640563200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100018493","name":"Taipei Medical University-Shuang Ho Hospital","doi-asserted-by":"publisher","award":["110TMU-SHH-13"],"award-info":[{"award-number":["110TMU-SHH-13"]}],"id":[{"id":"10.13039\/100018493","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology","doi-asserted-by":"publisher","award":["MOST109-2320-B-038-063 MOST109-2320-B-038-059, MOST110-2314-B-038-068 and MOST110-2320-B-038-052"],"award-info":[{"award-number":["MOST109-2320-B-038-063 MOST109-2320-B-038-059, MOST110-2314-B-038-068 and MOST110-2320-B-038-052"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["JCM"],"abstract":"<jats:p>Bicalutamide (Bic) is an androgen deprivation therapy (ADT) for treating prostate cancer, while ADT is potentially associated with acute kidney injury. Previously, we recognized Bic induced renal mitochondria dysfunction in vitro and in vivo via the ROS -HIF1\u03b1 pathway. Whether OXPHOS complex, as well as mitochondrial dynamics, can be influenced by Bic via modulation of peroxisome proliferator-activated receptor coactivator 1\u03b1 (PGC1\u03b1), NADPH oxidase 4 (Nox4), mitofusins 1\/2 (MFN 1\/2), optic atrophy 1 (OPA1), and sirtuins (SIRTs) has not been documented. Renal mesangial cell line was treated with Bic (30~60 \u03bcM) for the indicated time. SIRTs, complex I, mitochondrial dynamics- and oxidative stress-related proteins were analyzed. Bic dose-dependently reduced mitochondrial potential, but dose- and time-dependently suppressed translocase of the outer mitochondrial membrane member 20 (Tomm 20), complex I activity. Nox4 and glutathione lead to decreased NAD+\/NADH ratio, with upregulated superoxide dismutase 2. SIRT1 was initially stimulated and then suppressed, while SIRT3 was time- and dose-dependently downregulated. PGC1\u03b1, MFN2, and OPA1 were all upregulated, with MFN1 and pro-fission dynamin-related protein I downregulated. Bic exhibits potential to damage mitochondria via destroying complex I, complex I activity, and mitochondrial dynamics. Long-term treatment with Bic should be carefully followed up.<\/jats:p>","DOI":"10.3390\/jcm11010135","type":"journal-article","created":{"date-parts":[[2021,12,28]],"date-time":"2021-12-28T01:18:15Z","timestamp":1640654295000},"page":"135","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Bicalutamide Exhibits Potential to Damage Kidney via Destroying Complex I and Affecting Mitochondrial Dynamics"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5512-4631","authenticated-orcid":false,"given":"Kuan-Chou","family":"Chen","sequence":"first","affiliation":[{"name":"Graduate Institute of Clinical Medicine, School of Medicine, College of Medicine, Taipei Medical University, 250 Wu-Xing St., Sin-Yi District, Taipei 11031, Taiwan"},{"name":"Department of Urology, Taipei Medical University Shuang-Ho Hospital, New Taipei City 23561, Taiwan"},{"name":"TMU-Research Center of Urology and Kidney, Taipei Medical University, Taipei 11031, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang-Rong","family":"Chen","sequence":"additional","affiliation":[{"name":"International Medical Doctor Program, Vita-Salute San Raffaele University, 20132 Milan, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang-Yu","family":"Chen","sequence":"additional","affiliation":[{"name":"Program of Biomedical Sciences, College of Arts and Sciences, California Baptist University, Riverside, CA 92504, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiung-Chi","family":"Peng","sequence":"additional","affiliation":[{"name":"Graduate Institute of Clinical Medicine, School of Medicine, College of Medicine, Taipei Medical University, 250 Wu-Xing St., Sin-Yi District, Taipei 11031, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert Y.","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, College of Medical and Health Care, Hungkuang University, No. 1018, Sec. 6, Taiwan Boulevard, Shalu District, Taichung 43302, Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,12,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1152\/physrev.00020.2002","article-title":"Cell Biology of the Glomerular Podocyte","volume":"83","author":"Kriz","year":"2003","journal-title":"Physiol. 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