{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T17:21:41Z","timestamp":1787764901883,"version":"build-2784847793"},"reference-count":70,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,2,24]],"date-time":"2022-02-24T00:00:00Z","timestamp":1645660800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>The prevalence of chronic wounds is increasing due to the population aging and associated pathologies, such as diabetes. These ulcers have an important socio-economic impact. Thus, it is necessary to design new products for their treatment with an adequate cost\/effectiveness ratio. Among these products are amorphous hydrogels. Their composition can be manipulated to provide a favorable environment for ulcer healing. The aim of this study was to evaluate a novel multifunctional amorphous hydrogel (EHO-85), containing Olea europaea leaf extract, designed to enhance the wound healing process. For this purpose, its moistening ability, antioxidant capacity, effect on pH in the wound bed of experimental rats, and the effect on wound healing in a murine model of impaired wound healing were assessed. EHO-85 proved to be a remarkable moisturizer and its application in a rat skin wound model showed a significant antioxidant effect, decreasing lipid peroxidation in the wound bed. EHO-85 also decreased the pH of the ulcer bed from day 1. In addition, in mice (BKS. Cg-m +\/+ Leprdb) EHO-85 treatment showed superior wound healing rates compared to hydrocolloid dressing. In conclusion, EHO-85 can speed up the closure of hard-to-heal wounds due to its multifunctional properties that are able to modulate the wound microenvironment, mainly through its remarkable effect on reactive oxygen species, pH, and moistening regulation.<\/jats:p>","DOI":"10.3390\/jcm11051229","type":"journal-article","created":{"date-parts":[[2022,2,24]],"date-time":"2022-02-24T07:22:35Z","timestamp":1645687355000},"page":"1229","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["EHO-85: A Multifunctional Amorphous Hydrogel for Wound Healing Containing Olea europaea Leaf Extract: Effects on Wound Microenvironment and Preclinical Evaluation"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8520-8278","authenticated-orcid":false,"given":"Antonio","family":"Casado-D\u00edaz","sequence":"first","affiliation":[{"name":"Clinical Management Unit of Endocrinology and Nutrition, Reina Sof\u00eda University Hospital, University of C\u00f3rdoba, 14004 C\u00f3rdoba, Spain"},{"name":"Consortium for Biomedical Research in Frailty & Healthy Ageing, CIBERFES, Carlos III Institute of Health, 28029 Madrid, Spain"},{"name":"Maim\u00f3nides Institute of Biomedical Research (IMIBIC), Reina Sof\u00eda University Hospital, University of C\u00f3rdoba, 14004 C\u00f3rdoba, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"La Torre","sequence":"additional","affiliation":[{"name":"Maim\u00f3nides Institute of Biomedical Research (IMIBIC), Reina Sof\u00eda University Hospital, University of C\u00f3rdoba, 14004 C\u00f3rdoba, Spain"},{"name":"Department of Biochemistry and Molecular Biology, Faculty of Medicine and Nursing, University of C\u00f3rdoba, 14004 C\u00f3rdoba, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feliciano","family":"Priego-Capote","sequence":"additional","affiliation":[{"name":"Consortium for Biomedical Research in Frailty & Healthy Ageing, CIBERFES, Carlos III Institute of Health, 28029 Madrid, Spain"},{"name":"Maim\u00f3nides Institute of Biomedical Research (IMIBIC), Reina Sof\u00eda University Hospital, University of C\u00f3rdoba, 14004 C\u00f3rdoba, Spain"},{"name":"Department of Analytical Chemistry, Institute of Nanochemistry, University of C\u00f3rdoba, 14071 C\u00f3rdoba, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8307-7323","authenticated-orcid":false,"given":"Jos\u00e9","family":"Verd\u00fa-Soriano","sequence":"additional","affiliation":[{"name":"Department of Community Nursing, Preventive Medicine, Public Health and History of Science, Faculty of Health Sciences, University of Alicante, 03690 Alicante, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6110-0265","authenticated-orcid":false,"given":"Jos\u00e9 Luis","family":"L\u00e1zaro-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Diabetic Foot Unit, University Podiatry Clinic, Complutense University of Madrid, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leocadio","family":"Rodr\u00edguez-Ma\u00f1as","sequence":"additional","affiliation":[{"name":"Consortium for Biomedical Research in Frailty & Healthy Ageing, CIBERFES, Carlos III Institute of Health, 28029 Madrid, Spain"},{"name":"Department of Geriatrics, Hospital Universitario de Getafe, 28905 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Berenguer P\u00e9rez","sequence":"additional","affiliation":[{"name":"Department of Community Nursing, Preventive Medicine, Public Health and History of Science, Faculty of Health Sciences, University of Alicante, 03690 Alicante, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4392-9122","authenticated-orcid":false,"given":"Isaac","family":"Tunez","sequence":"additional","affiliation":[{"name":"Maim\u00f3nides Institute of Biomedical Research (IMIBIC), Reina Sof\u00eda University Hospital, University of C\u00f3rdoba, 14004 C\u00f3rdoba, Spain"},{"name":"Department of Biochemistry and Molecular Biology, Faculty of Medicine and Nursing, University of C\u00f3rdoba, 14004 C\u00f3rdoba, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,2,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1111\/j.1524-475X.2009.00543.x","article-title":"Human skin wounds: A major and snowballing threat to public health and the economy","volume":"17","author":"Sen","year":"2009","journal-title":"Wound Repair 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