{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T19:59:42Z","timestamp":1791662382512,"version":"4.3.4"},"reference-count":46,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,3,25]],"date-time":"2022-03-25T00:00:00Z","timestamp":1648166400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Background: To identify the correlation among female age, cellular aging markers, and aneuploidy rate in in vitro fertilization (IVF) and the preimplantation genetic test for aneuploidy (PGT-A) cycles. Methods: This is a prospective cohort study recruiting 110 infertile women between August 2017 and July 2018. They were divided into young-age (&lt;38 years, n = 60) and advanced-age (\u226538 years, n = 50) groups. Peripheral leukocytes were assessed, and the granulosa cells were pooled during oocyte pickup. Mitochondrial DNA (mtDNA) copy number and telomere length (TL) were measured using real-time polymerase chain reaction. PGT-A was performed on the NGS platform. Results: mtDNA copy number and TL were positively correlated in both leukocytes (rho = 0.477, p &lt; 0.001) and granulosa cells (rho = 0.361, p &lt; 0.001), but the two parameters in leukocytes were not correlated with those in granulosa cells. In the young-age group, TL in the granulosa cells was the only factor correlated with the aneuploidy rate (rho = \u22120.283, p = 0.044), whereas in the advanced-age group, age was the main factor (rho = 0.358, p = 0.018). Conclusions: TL in the granulosa cells was negatively correlated with the aneuploidy rate in the young-age group, supporting the application of PGT-A in younger women.<\/jats:p>","DOI":"10.3390\/jcm11071824","type":"journal-article","created":{"date-parts":[[2022,3,27]],"date-time":"2022-03-27T21:27:51Z","timestamp":1648416471000},"page":"1824","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Assessment of Telomere Length and Mitochondrial DNA Copy Number in Granulosa Cells as Predictors of Aneuploidy Rate in Young Patients"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9253-7037","authenticated-orcid":false,"given":"Tzu-Ning","family":"Yu","sequence":"first","affiliation":[{"name":"Institute of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan"},{"name":"Division of Infertility, Lee Women\u2019s Hospital, Taichung 40652, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"En-Hui","family":"Cheng","sequence":"additional","affiliation":[{"name":"Division of Infertility, Lee Women\u2019s Hospital, Taichung 40652, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han-Ni","family":"Tsai","sequence":"additional","affiliation":[{"name":"Division of Infertility, Lee Women\u2019s Hospital, Taichung 40652, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pin-Yao","family":"Lin","sequence":"additional","affiliation":[{"name":"Division of Infertility, Lee Women\u2019s Hospital, Taichung 40652, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6352-3437","authenticated-orcid":false,"given":"Chien-Hong","family":"Chen","sequence":"additional","affiliation":[{"name":"Division of Infertility, Lee Women\u2019s Hospital, Taichung 40652, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun-Chia","family":"Huang","sequence":"additional","affiliation":[{"name":"Division of Infertility, Lee Women\u2019s Hospital, Taichung 40652, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8858-0916","authenticated-orcid":false,"given":"Tsung-Hsien","family":"Lee","sequence":"additional","affiliation":[{"name":"Institute of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan"},{"name":"Division of Infertility, Lee Women\u2019s Hospital, Taichung 40652, Taiwan"},{"name":"Department of Obstetrics and Gynecology, Chung Shan Medical University Hospital, Taichung 40201, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maw-Sheng","family":"Lee","sequence":"additional","affiliation":[{"name":"Institute of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan"},{"name":"Division of Infertility, Lee Women\u2019s Hospital, Taichung 40652, Taiwan"},{"name":"Department of Obstetrics and Gynecology, Chung Shan Medical University Hospital, Taichung 40201, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,3,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1093\/humupd\/dms043","article-title":"The ageing ovary and uterus: New biological insights","volume":"19","author":"Nelson","year":"2013","journal-title":"Hum. 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