{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T02:46:25Z","timestamp":1762051585892,"version":"build-2065373602"},"reference-count":45,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2022,5,8]],"date-time":"2022-05-08T00:00:00Z","timestamp":1651968000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Claes Frostell Research and Consulting AB, Stockholm, Sweden","award":["SUB.Z.A170.22.073","UMO-2016\/23\/D\/NZ6\/02554"],"award-info":[{"award-number":["SUB.Z.A170.22.073","UMO-2016\/23\/D\/NZ6\/02554"]}]},{"name":"National Science Centre","award":["SUB.Z.A170.22.073","UMO-2016\/23\/D\/NZ6\/02554"],"award-info":[{"award-number":["SUB.Z.A170.22.073","UMO-2016\/23\/D\/NZ6\/02554"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Inhaled nitric oxide (iNO) remains one of the treatment modalities in shock, and in addition to its vasoactive properties, iNO exerts immunomodulatory effects. We used a porcine model of endotoxemia with shock resuscitation (control) and additional treatment with iNO and a steroid (treatment group). After 20 h, bone marrow (BM), peripheral blood (PB), and bronchoalveolar lavage fluid (BALF) were collected to analyze the immunophenotype and mitochondrial membrane potential (\u0394\u03c6) in three subsets of monocytes. In both groups, SLA-DR expression decreased twofold on the circulating CD14+CD163+ and CD14\u2212CD163+ monocytes, while it did not change on the CD14+CD163+. \u0394\u03c6 increased only in the CD14\u2212CD163+ subpopulation (0.8 vs. 2.0, p &lt; 0.001). The analysis of compartment-specific alterations showed that nearly 100% of BALF CD14+CD163+ and CD14\u2212CD163+ monocytes expressed SLA-DR, and it was higher compared to PB (32% and 20%, p &lt; 0.0001) and BM (93% and 67%, p &lt; 0.001, respectively) counterparts. BALF CD14+CD163+ had a threefold higher \u0394\u03c6 than PB and BM monocytes, while the \u0394\u03c6 of the other subsets was highest in PB monocytes. We confirmed the compartmentalization of the monocyte response during endotoxemic shock, which highlights the importance of studying tissue-resident cells in addition to their circulating counterparts. The iNO\/steroid treatment did not further impair monocyte fitness.<\/jats:p>","DOI":"10.3390\/jcm11092641","type":"journal-article","created":{"date-parts":[[2022,5,8]],"date-time":"2022-05-08T08:04:21Z","timestamp":1651997061000},"page":"2641","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Compartment-Specific Differences in the Activation of Monocyte Subpopulations Are Not Affected by Nitric Oxide and Glucocorticoid Treatment in a Model of Resuscitated Porcine Endotoxemic Shock"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6233-7758","authenticated-orcid":false,"given":"Tomasz","family":"Skirecki","sequence":"first","affiliation":[{"name":"Laboratory of Flow Cytometry, Centre of Postgraduate Medical Education Marymoncka 99\/103, 01-813 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Adamik","sequence":"additional","affiliation":[{"name":"Clinical Department of the Anaesthesiology and Intensive Therapy, Wroclaw Medical University, 50-367 Wroclaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7199-1310","authenticated-orcid":false,"given":"Claes","family":"Frostell","sequence":"additional","affiliation":[{"name":"Department of Anaesthesia and Intensive Care, Karolinska Institutet, Danderyd Hospital, 182 57 Stockholm, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2298-9713","authenticated-orcid":false,"given":"Urszula","family":"Pas\u0142awska","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Torun, 87-100 Torun, Poland"},{"name":"Department of Internal Medicine and Clinic for Horses, Dogs and Cats, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stanis\u0142aw","family":"Zieli\u0144ski","sequence":"additional","affiliation":[{"name":"Clinical Department of the Anaesthesiology and Intensive Therapy, Wroclaw Medical University, 50-367 Wroclaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia","family":"Glatzel-Pluci\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-367 Wroclaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mateusz","family":"Olbromski","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-367 Wroclaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Piotr","family":"Dzi\u0119giel","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, 50-367 Wroclaw, Poland"},{"name":"Department of Physiotherapy, University School of Physical Education, 51-612 Wroclaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8668-1641","authenticated-orcid":false,"given":"Waldemar","family":"Gozdzik","sequence":"additional","affiliation":[{"name":"Clinical Department of the Anaesthesiology and Intensive Therapy, Wroclaw Medical University, 50-367 Wroclaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,5,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"414","DOI":"10.1056\/NEJMp1707170","article-title":"Recognizing Sepsis as a Global Health Priority\u2014A WHO Resolution","volume":"377","author":"Reinhart","year":"2017","journal-title":"N. 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