{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T14:47:36Z","timestamp":1783435656606,"version":"3.54.6"},"reference-count":26,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,6,7]],"date-time":"2022-06-07T00:00:00Z","timestamp":1654560000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100017515","name":"Heidelberger Stiftung Chirurgie","doi-asserted-by":"publisher","award":["2017\/170"],"award-info":[{"award-number":["2017\/170"]}],"id":[{"id":"10.13039\/501100017515","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Gene expression profiling of abdominal aortic aneurysms (AAA) indicates that chronic inflammatory responses, active matrix metalloproteinases, and degradation of the extracellular matrix components are involved in disease development and progression. This study investigates intra- and interpersonal RNA genome-wide expression profiling differences (Illumina HumanHT-12, BeadCHIP expression) of 24 AAA biopsies from 12 patients using a single gene and pathway (GeneOntology, GO enrichment) analysis. Biopsies were collected during open surgical AAA repair and according to prior finite element analysis (FEA) from regions with the highest and lowest wall stress. Single gene analysis revealed a strong heterogeneity of RNA expression parameters within the same and different AAA biopsies. The pathway analysis of all samples showed significant enrichment of genes from three different signaling pathways (integrin signaling pathway: fold change FC 1.63, p = 0.001; cholecystokinin receptor pathway: FC 1.60, p = 0.011; inflammation mediated by chemokine signaling pathway: FC 1.45, p = 0.028). These results indicate heterogeneous gene expression patterns within the AAA vascular wall. Single biopsy investigations do not permit a comprehensive characterization of activated molecular processes in AAA disease.<\/jats:p>","DOI":"10.3390\/jcm11123260","type":"journal-article","created":{"date-parts":[[2022,6,10]],"date-time":"2022-06-10T02:25:33Z","timestamp":1654827933000},"page":"3260","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Gene Expression Profiling in Abdominal Aortic Aneurysms"],"prefix":"10.3390","volume":"11","author":[{"given":"Amelie L.","family":"Behrens","sequence":"first","affiliation":[{"name":"Department of Vascular and Endovascular Surgery, University Hospital of Heidelberg, 69120 Heidelberg, Germany"},{"name":"Department of Vascular and Endovascular Surgery, University Hospital Cologne, 50937 K\u00f6ln, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Dihlmann","sequence":"additional","affiliation":[{"name":"Department of Vascular and Endovascular Surgery, University Hospital of Heidelberg, 69120 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caspar","family":"Grond-Ginsbach","sequence":"additional","affiliation":[{"name":"Department of Vascular and Endovascular Surgery, University Hospital of Heidelberg, 69120 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas S.","family":"Peters","sequence":"additional","affiliation":[{"name":"Department of Vascular and Endovascular Surgery, University Hospital of Heidelberg, 69120 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8262-8381","authenticated-orcid":false,"given":"Bernhard","family":"Dorweiler","sequence":"additional","affiliation":[{"name":"Department of Vascular and Endovascular Surgery, University Hospital Cologne, 50937 K\u00f6ln, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dittmar","family":"B\u00f6ckler","sequence":"additional","affiliation":[{"name":"Department of Vascular and Endovascular Surgery, University Hospital of Heidelberg, 69120 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4964-2448","authenticated-orcid":false,"given":"Philipp","family":"Erhart","sequence":"additional","affiliation":[{"name":"Department of Vascular and Endovascular Surgery, University Hospital of Heidelberg, 69120 Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1016\/j.jvs.2010.05.090","article-title":"Analysis of risk factors for abdominal aortic aneurysm in a cohort of more than 3 million individuals","volume":"52","author":"Kent","year":"2010","journal-title":"J. Vasc. Surg."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.jvs.2021.07.125","article-title":"The natural history of large abdominal aortic aneurysms in patients without timely repair","volume":"75","author":"Lancaster","year":"2022","journal-title":"J Vasc. Surg."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1007\/978-3-319-96445-4_8","article-title":"Abdominal Aortic Aneurysm Pathomechanics: Current Understanding and Future Directions","volume":"1097","author":"Kemmerling","year":"2018","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_4","first-page":"161","article-title":"Autopsy study of unoperated abdominal aortic aneurysms. The case for early resection","volume":"56","author":"Darling","year":"1977","journal-title":"Circulation"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1038\/nrcardio.2009.80","article-title":"Biomarkers of AAA progression. Part 1: Extracellular matrix degeneration","volume":"6","author":"Hellenthal","year":"2009","journal-title":"Nat. Rev. Cardiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"230","DOI":"10.2119\/molmed.2013.00162","article-title":"Increased Expression and activation of Absent in Melanoma 2 Inflammasome components in lymphocytic infiltrates of abdominal aortic aneurysms","volume":"20","author":"Dihlmann","year":"2014","journal-title":"Mol. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1093\/cvr\/cvq337","article-title":"Novel aspects of the pathogenesis of aneurysms of the abdominal aorta in humans","volume":"90","author":"Michel","year":"2011","journal-title":"Cardiovasc. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1159\/000228900","article-title":"Inflammatory infiltrates and neovessels are relevant sources of MMPs in abdominal aortic aneurysm wall","volume":"76","author":"Reeps","year":"2009","journal-title":"Pathobiology"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1448","DOI":"10.1161\/CIRCRESAHA.114.304936","article-title":"Role of mechanotransduction in vascular biology: Focus on thoracic aortic aneurysms and dissections","volume":"116","author":"Humphrey","year":"2015","journal-title":"Circ. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1177\/1526602817729165","article-title":"Gene Expression Profiling in Abdominal Aortic Aneurysms After Finite Element Rupture Risk Assessment","volume":"24","author":"Erhart","year":"2017","journal-title":"J. Endovasc. Ther."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1583\/14-4695.1","article-title":"Finite element analysis of abdominal aortic aneurysms: Predicted rupture risk correleates with aortic wall histology in individual patients","volume":"21","author":"Erhart","year":"2014","journal-title":"J. Endovasc. Ther."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1038\/75556","article-title":"Gene ontology: Tool for the unification of biology. The Gene Ontology Consortium","volume":"25","author":"Ashburner","year":"2000","journal-title":"Nat. Genet."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Gene Ontology Consortium (2021). The Gene Ontology resource: Enriching a GOld mine. Nucleic Acids Res., 49, 325\u2013334.","DOI":"10.1093\/nar\/gkaa1113"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1093\/nar\/gkaa1106","article-title":"PANTHER version 16: A revised family classification, tree-based classification tool, enhancer regions and extensive API","volume":"49","author":"Mi","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Safran, M., Rosen, N., Twik, M., BarShir, R., Stein, T.I., Dahary, D., Fishilevich, S., and Lancet, D. (2021). The GeneCards Suite. Practical Guide to Life Science Databases, Springer.","DOI":"10.1007\/978-981-16-5812-9_2"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"385","DOI":"10.33549\/physiolres.933905","article-title":"Gene expression in patients with abdominal aortic aneurysm-more than immunological mechanisms involved","volume":"68","author":"Prucha","year":"2019","journal-title":"Physiol Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1083\/jcb.201609037","article-title":"Integrin-mediated mechanotransduction","volume":"215","author":"Sun","year":"2016","journal-title":"J. Cell Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2023","DOI":"10.1016\/j.jvs.2021.06.020","article-title":"Sex hormones in men with abdominal aortic aneurysm","volume":"74","author":"Villard","year":"2021","journal-title":"J. Vasc. Surg."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.ejvs.2010.04.014","article-title":"Abdominal aortic aneurysm rupture is not associated with an Up-regulation of inflammation within the aneurysm wall","volume":"40","author":"Wilson","year":"2010","journal-title":"Eur. J. Vasc. Endovasc. Surg."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.jvs.2011.06.113","article-title":"Circumferential heterogeneity in the abdominal aortic aneurysm wall composition suggests lateral sides to be more rupture prone","volume":"55","author":"Hurks","year":"2012","journal-title":"J. Vasc. Surg."},{"key":"ref_21","first-page":"4623","article-title":"The expression of MMP-7 in serum and aneurysm tissues of patients with abdominal aortic aneurysm associated with hypertension and the clinical efficacy of endovascular exclusion","volume":"21","author":"Zhang","year":"2017","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1007\/s100169900144","article-title":"Expression of matrix metalloproteinases and TIMPs in human abdominal aortic aneurysms","volume":"12","author":"Elmore","year":"1998","journal-title":"Ann. Vasc. Surg."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1016\/j.surg.2004.12.004","article-title":"MMP-12 has a role in abdominal aortic aneurysms in mice","volume":"137","author":"Longo","year":"2005","journal-title":"Surgery"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1186\/s12872-018-0931-0","article-title":"Serum matrix metalloproteinase-9 is a valuable biomarker for identification of abdominal and thoracic aortic aneurysm: A case-control study","volume":"18","author":"Li","year":"2018","journal-title":"BMC Cardiovasc. Disord."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.ijcard.2014.03.138","article-title":"PPAR\u03b4 reduces abdominal aortic aneurysm formation in angiotensin II-infused apolipoprotein E-deficient mice by regulating extracellular matrix homeostasis and inflammatory responses","volume":"174","author":"Hwang","year":"2014","journal-title":"Int. J. Cardiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/1472-6793-11-9","article-title":"Regional expression of HOX4A along the aorta and ist potential role in human abdominal aortic aneurysms","volume":"11","author":"Lillvis","year":"2011","journal-title":"BMC Physiol."}],"container-title":["Journal of Clinical Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2077-0383\/11\/12\/3260\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:25:39Z","timestamp":1760138739000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2077-0383\/11\/12\/3260"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,7]]},"references-count":26,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2022,6]]}},"alternative-id":["jcm11123260"],"URL":"https:\/\/doi.org\/10.3390\/jcm11123260","relation":{},"ISSN":["2077-0383"],"issn-type":[{"value":"2077-0383","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,7]]}}}