{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T18:57:17Z","timestamp":1775847437556,"version":"3.50.1"},"reference-count":49,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,6,7]],"date-time":"2022-06-07T00:00:00Z","timestamp":1654560000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Medical University of Lublin, Lublin, Poland","award":["DS 403-WFJ"],"award-info":[{"award-number":["DS 403-WFJ"]}]},{"name":"the Medical University of Lublin, Lublin, Poland","award":["DS 222-JK"],"award-info":[{"award-number":["DS 222-JK"]}]},{"name":"the Medical University of Lublin, Lublin, Poland","award":["T3-RP"],"award-info":[{"award-number":["T3-RP"]}]},{"name":"the Mossakowski Medical Research Institute, Polish Academy of Sciences, Warsaw, Poland","award":["DS 403-WFJ"],"award-info":[{"award-number":["DS 403-WFJ"]}]},{"name":"the Mossakowski Medical Research Institute, Polish Academy of Sciences, Warsaw, Poland","award":["DS 222-JK"],"award-info":[{"award-number":["DS 222-JK"]}]},{"name":"the Mossakowski Medical Research Institute, Polish Academy of Sciences, Warsaw, Poland","award":["T3-RP"],"award-info":[{"award-number":["T3-RP"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>In this study, the expression of the genes of the amyloid protein precursor, \u03b2-secretase, presenilin 1 and 2 by RT-PCR in the lymphocytes of newborns after perinatal asphyxia and perinatal asphyxia treated with hypothermia was analyzed at the age of 15\u201321 days. The relative quantification of Alzheimer\u2019s-disease-related genes was first performed by comparing the peripheral lymphocytes of non-asphyxia control versus those with asphyxia or asphyxia with hypothermia. In the newborns who had perinatal asphyxia, the peripheral lymphocytes presented a decreased expression of the amyloid protein precursor and \u03b2-secretase genes. On the other hand, the expression of the presenilin 1 and 2 genes increased in the studied group. The expression of the studied genes in the asphyxia group treated with hypothermia had an identical pattern of changes that were not statistically significant to the asphyxia group. This suggests that the expression of the genes involved in the metabolism of the amyloid protein precursor in the peripheral lymphocytes may be a biomarker of progressive pathological processes in the brain after asphyxia that are not affected by hypothermia. These are the first data in the world showing the role of hypothermia in the gene changes associated with Alzheimer\u2019s disease in the peripheral lymphocytes of newborns after asphyxia.<\/jats:p>","DOI":"10.3390\/jcm11123263","type":"journal-article","created":{"date-parts":[[2022,6,10]],"date-time":"2022-06-10T02:25:33Z","timestamp":1654827933000},"page":"3263","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Hypothermia after Perinatal Asphyxia Does Not Affect Genes Responsible for Amyloid Production in Neonatal Peripheral Lymphocytes"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9369-4213","authenticated-orcid":false,"given":"Agata","family":"Tarkowska","sequence":"first","affiliation":[{"name":"Department of Neonate and Infant Pathology, Medical University of Lublin, 20-093 Lublin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanda","family":"Furmaga-Jab\u0142o\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Neonate and Infant Pathology, Medical University of Lublin, 20-093 Lublin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8683-545X","authenticated-orcid":false,"given":"Jacek","family":"Bogucki","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry, Faculty of Pharmacy, Medical University of Lublin, 20-093 Lublin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janusz","family":"Kocki","sequence":"additional","affiliation":[{"name":"Department of Clinical Genetics, Medical University of Lublin, 20-080 Lublin, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0764-1356","authenticated-orcid":false,"given":"Ryszard","family":"Pluta","sequence":"additional","affiliation":[{"name":"Laboratory of Ischemic and Neurodegenerative Brain Research, Mossakowski Medical Research Institute, Polish Academy of Sciences, 02-106 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,7]]},"reference":[{"key":"ref_1","unstructured":"Boldingh, A.M., Solev\u00e5g, A.L., and Nakstad, B. 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