{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T05:23:12Z","timestamp":1762060992186,"version":"build-2065373602"},"reference-count":36,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2022,8,10]],"date-time":"2022-08-10T00:00:00Z","timestamp":1660089600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>The pathophysiological mechanisms of noise-induced hearing loss remain unknown. Identifying biomarkers of noise-induced hearing loss may increase the understanding of pathophysiological mechanisms of deafness, allow for a more precise diagnosis, and inform personalized treatment. Emerging techniques such as metabolomics can help to identify these biomarkers. The objective of the present study was to investigate immediate-early changes in the perilymph metabolome following acoustic trauma. Metabolomic analysis was performed using liquid chromatography coupled to mass spectrophotometry to analyze metabolic changes in perilymph associated with noise-induced hearing loss. Sheep (n = 6) were exposed to a noise designed to induce substantial hearing loss. Perilymph was collected before and after acoustic trauma. Data were analyzed using univariate analysis and a supervised multivariate analysis based on partial least squares discriminant analysis. A metabolomic analysis showed an abundance of 213 metabolites. Four metabolites were significantly changed following acoustic trauma (Urocanate (p = 0.004, FC = 0.48), S-(5\u2019-Adenosyl)-L-Homocysteine (p = 0.06, FC = 2.32), Trigonelline (p = 0.06, FC = 0.46) and N-Acetyl-L-Leucine (p = 0.09, FC = 2.02)). The approach allowed for the identification of new metabolites and metabolic pathways involved with acoustic trauma that were associated with auditory impairment (nerve damage, mechanical destruction, and oxidative stress). The results suggest that metabolomics provides a powerful approach to characterize inner ear metabolites which may lead to identification of new therapies and therapeutic targets.<\/jats:p>","DOI":"10.3390\/jcm11164668","type":"journal-article","created":{"date-parts":[[2022,8,10]],"date-time":"2022-08-10T04:20:32Z","timestamp":1660105232000},"page":"4668","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Immediate-Early Modifications to the Metabolomic Profile of the Perilymph Following an Acoustic Trauma in a Sheep Model"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7034-4680","authenticated-orcid":false,"given":"Luc","family":"Boullaud","sequence":"first","affiliation":[{"name":"ENT Department and Cervico-Facial Surgery, CHU de Tours, 2 Boulevard Tonnell\u00e9, 37044 Tours, France"},{"name":"INSERM U1253, iBrain, University of Tours, 10 Boulevard Tonnell\u00e9, 37000 Tours, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00e9l\u00e8ne","family":"Blasco","sequence":"additional","affiliation":[{"name":"INSERM U1253, iBrain, University of Tours, 10 Boulevard Tonnell\u00e9, 37000 Tours, France"},{"name":"Department of Biochemistry and Molecular Biology, CHU de Tours, 2 Boulevard Tonnell\u00e9, 37044 Tours, France"},{"name":"Faculty of Medecine, University of Tours, 10 Boulevard Tonnell\u00e9, 37000 Tours, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eliott","family":"Caillaud","sequence":"additional","affiliation":[{"name":"ENT Department and Cervico-Facial Surgery, CHU de Tours, 2 Boulevard Tonnell\u00e9, 37044 Tours, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5324-2164","authenticated-orcid":false,"given":"Patrick","family":"Emond","sequence":"additional","affiliation":[{"name":"INSERM U1253, iBrain, University of Tours, 10 Boulevard Tonnell\u00e9, 37000 Tours, France"},{"name":"Faculty of Medecine, University of Tours, 10 Boulevard Tonnell\u00e9, 37000 Tours, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Bakhos","sequence":"additional","affiliation":[{"name":"ENT Department and Cervico-Facial Surgery, CHU de Tours, 2 Boulevard Tonnell\u00e9, 37044 Tours, France"},{"name":"INSERM U1253, iBrain, University of Tours, 10 Boulevard Tonnell\u00e9, 37000 Tours, France"},{"name":"Faculty of Medecine, University of Tours, 10 Boulevard Tonnell\u00e9, 37000 Tours, France"},{"name":"House Institute Foundation, Los Angeles, CA 90089, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,10]]},"reference":[{"key":"ref_1","unstructured":"World Health Organisation (2022, June 03). 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