{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T06:57:34Z","timestamp":1762066654061,"version":"build-2065373602"},"reference-count":49,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2022,9,17]],"date-time":"2022-09-17T00:00:00Z","timestamp":1663372800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Italian Ministry of Health"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Background: Endoscopic ultrasound (EUS) plays an important role in the diagnosis and staging of thoracic disease. Our report studies the diagnostic performance and clinical impact of EUS fine needle aspiration (FNA) in a homogenous cohort of patients according to the distribution of the enlarged MLNs or pulmonary masses. Methods: We retrospectively reviewed the diagnostic performance of 211 EUS-FNA in 200 consecutive patients with enlarged or PET-positive MLNs and para-mediastinal masses who were referred to our oncological center between January 2019 and May 2020. Results: The overall sensitivity of EUS-FNA was 85% with a corresponding negative predictive value (NPV) of 56% and an accuracy of 87.5%. The sensitivity and accuracy in patients with abnormal MLNs were 81.1% and 84.4%, respectively. In those with para-mediastinal masses, sensitivity and accuracy were 96.4% and 96.8%. The accuracy for both masses and lymph nodes was 100%, and in the LAG (left adrenal gland), it was 66.6%. Conclusions: Our results show that, in patients with suspected mediastinal masses, EUS-FNA is an accurate technique to evaluate all reachable mediastinal nodal stations, including station 5.<\/jats:p>","DOI":"10.3390\/jcm11185469","type":"journal-article","created":{"date-parts":[[2022,9,19]],"date-time":"2022-09-19T02:06:50Z","timestamp":1663553210000},"page":"5469","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Transesophageal Endoscopic Ultrasound Fine Needle Biopsy for the Diagnosis of Mediastinal Masses: A Retrospective Real-World Analysis"],"prefix":"10.3390","volume":"11","author":[{"given":"Daniela","family":"Assisi","sequence":"first","affiliation":[{"name":"Digestive Endoscopy Unit, IRCCS Regina Elena National Cancer Institute, 00144 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5579-0837","authenticated-orcid":false,"given":"Filippo Tommaso","family":"Gallina","sequence":"additional","affiliation":[{"name":"Thoracic Surgery Unit, IRCCS Regina Elena National Cancer Institute, 00144 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniele","family":"Forcella","sequence":"additional","affiliation":[{"name":"Thoracic Surgery Unit, IRCCS Regina Elena National Cancer Institute, 00144 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Riccardo","family":"Taj\u00e8","sequence":"additional","affiliation":[{"name":"Thoracic Surgery Unit, IRCCS Regina Elena National Cancer Institute, 00144 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enrico","family":"Melis","sequence":"additional","affiliation":[{"name":"Thoracic Surgery Unit, IRCCS Regina Elena National Cancer Institute, 00144 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo","family":"Visca","sequence":"additional","affiliation":[{"name":"Department of Pathology, IRCCS Regina Elena National Cancer Institute, 00144 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federico","family":"Pierconti","sequence":"additional","affiliation":[{"name":"Anesthesiology and Intensive Care Unit, IRCCS Regina Elena National Cancer Institute, 00144 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emanuela","family":"Venti","sequence":"additional","affiliation":[{"name":"Anesthesiology and Intensive Care Unit, IRCCS Regina Elena National Cancer Institute, 00144 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7137-8946","authenticated-orcid":false,"given":"Francesco","family":"Facciolo","sequence":"additional","affiliation":[{"name":"Thoracic Surgery Unit, IRCCS Regina Elena National Cancer Institute, 00144 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1097\/LBR.0000000000000552","article-title":"A Multicenter Study on the Utility of EBUS-TBNA and EUS-B-FNA in the Diagnosis of Mediastinal Lymphoma","volume":"26","author":"Dhooria","year":"2019","journal-title":"J. Bronchol. Interv. Pulmonol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"6960","DOI":"10.21037\/jtd.2018.11.35","article-title":"Biopsy needles for mediastinal lymph node sampling by endosonography: Current knowledge and future perspectives","volume":"10","author":"Colella","year":"2018","journal-title":"J. Thorac. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"863","DOI":"10.1080\/17476348.2019.1646642","article-title":"Role of endobronchial ultrasound-guided transbronchial needle aspiration in lung cancer management","volume":"13","author":"Sakairi","year":"2019","journal-title":"Expert Rev. Respir. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1097\/JTO.0b013e31818020eb","article-title":"The International Epidemiology of Lung Cancer Geographical Distribution and Secular Trends","volume":"3","author":"Youlden","year":"2008","journal-title":"J. Thorac. Oncol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1177\/107327481402100102","article-title":"Lung cancer screening: Advantages, controversies, and applications","volume":"21","author":"Nanavaty","year":"2014","journal-title":"Cancer Control"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"15009","DOI":"10.1038\/nrdp.2015.9","article-title":"Non-small-cell lung cancer","volume":"1","author":"Gridelli","year":"2015","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/j.lungcan.2017.08.011","article-title":"Timeliness of access to lung cancer diagnosis and treatment: A scoping literature review","volume":"112","author":"Jacobsen","year":"2017","journal-title":"Lung Cancer"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"4515","DOI":"10.1007\/s00330-016-4292-8","article-title":"The diagnostic ability of 18F-FDG PET\/CT for mediastinal lymph node staging using 18F-FDG uptake and volumetric CT histogram analysis in non-small cell lung cancer","volume":"26","author":"Lee","year":"2016","journal-title":"Eur. Radiol."},{"key":"ref_9","first-page":"CD009519","article-title":"PET-CT for assessing mediastinal lymph node involvement in patients with suspected resectable non-small cell lung cancer","volume":"11","author":"Baldwin","year":"2014","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3401","DOI":"10.1007\/s00330-016-4718-3","article-title":"CT-guided core needle biopsy of mediastinal nodes through a transpulmonary approach: Retrospective analysis of the procedures conducted over six years","volume":"27","author":"Yin","year":"2017","journal-title":"Eur. Radiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1016\/j.diii.2016.06.020","article-title":"FDG PET-CT for solitary pulmonary nodule and lung cancer: Literature review","volume":"97","author":"Groheux","year":"2016","journal-title":"Diagn Interv. Imaging"},{"key":"ref_12","first-page":"199","article-title":"Positron emission tomography\/computed tomography (PET\/CT) and CT for N staging of non-small cell lung cancer","volume":"14","year":"2017","journal-title":"Med Glas"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.lungcan.2017.04.018","article-title":"Indications for invasive mediastinal staging in patients with early non-small cell lung cancer staged with PET-CT","volume":"109","author":"Gao","year":"2017","journal-title":"Lung Cancer"},{"key":"ref_14","first-page":"225","article-title":"Preoperative mediastinal lymph node staging for non-small cell lung cancer:2014 update of the 2007 ESTS guidelines","volume":"3","author":"Dooms","year":"2014","journal-title":"Transl. Lung Cancer Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1720","DOI":"10.1016\/S0003-4975(02)03821-3","article-title":"Biopsy of anterior mediastinal masses under local anesthesia","volume":"74","author":"Rendina","year":"2002","journal-title":"Ann. Thorac. Surg."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1124","DOI":"10.1016\/j.ejcts.2008.03.014","article-title":"Does endoesophageal ultrasound-guided fine-needle aspiration replace mediastinoscopy in mediastinal staging of thoracic malignancies?","volume":"33","author":"Witte","year":"2008","journal-title":"Eur. J. Cardio-Thorac. Surg."},{"key":"ref_17","first-page":"28","article-title":"Contribution of endobronchial ultrasound elastography to the characterization of mediastinal lymphadenopathy: A single-center, prospective, observational study","volume":"76","author":"Jeny","year":"2019","journal-title":"Respir. Med. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3004","DOI":"10.1378\/chest.128.4.3004","article-title":"Esophageal endoscopic ultrasound with fine-needle aspiration with on-site pathologist:high accuracy for the diagnosis of mediastinal lymphadenopathy","volume":"128","author":"Tournoy","year":"2005","journal-title":"Chest"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"13","DOI":"10.2217\/fon-2017-0387","article-title":"N2 disease in non-small-cell lung cancer: Straight to surgery?","volume":"14","author":"Guzman","year":"2018","journal-title":"Future Oncol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/j.athoracsur.2017.02.031","article-title":"Surgically Managed Clinical Stage IIIA-Clinical N2 Lung Cancer in The Society of Thoracic Surgeons Database","volume":"104","author":"Boffa","year":"2017","journal-title":"Ann. Thorac. Surg."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"8357","DOI":"10.1200\/JCO.2005.01.1965","article-title":"Endoscopic ultrasound-guided fine-needle aspiration in the diagnosis and staging of lung cancer and its impact on surgical staging","volume":"23","author":"Annema","year":"2005","journal-title":"J. Clin. Oncol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1378\/chest.123.1_suppl.157S","article-title":"Invasive staging of non-small cell lung cancer: A review of the current evidence","volume":"123","author":"Toloza","year":"2003","journal-title":"Chest"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1080\/028418601750071109","article-title":"Mediastinoscopy: Its role and value today in the differential mediastinal pathology","volume":"40","author":"Hujala","year":"2001","journal-title":"Acta Oncol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1747","DOI":"10.1016\/j.athoracsur.2016.05.110","article-title":"Endosonography Versus Mediastinoscopy in Mediastinal Staging of Lung Cancer: Systematic Review and Meta-Analysis","volume":"102","author":"Sehgal","year":"2016","journal-title":"Ann. Thorac. Surg."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"18","DOI":"10.3310\/hta16180","article-title":"Clinical effectiveness and cost-effectiveness of endobronchial and endoscopic ultrasound relative to surgical staging in potentially resectable lung cancer: Results from the ASTER randomized controlled trial. Health technol Assess","volume":"16","author":"Sharples","year":"2012","journal-title":"Health Technol. Assess."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"13","DOI":"10.21037\/med-20-53","article-title":"Five years of thoracic endoscopy unit activity on lung cancer staging: How teamwork can improve the outcomes","volume":"5","author":"Gallina","year":"2021","journal-title":"Mediastinum"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.lungcan.2005.04.005","article-title":"Endoscopic ultrasound guided biopsy performed routinely in lung cancer staging spares futile thoracotomies: Preliminary results from a randomized clinical trial","volume":"49","author":"Larsen","year":"2005","journal-title":"Lung Cancer"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/j.ejca.2003.11.010","article-title":"Endoscopic ultrasound-guided fine-needle aspiration in patients with mediastinal abnormalities and previous extrathoracic malignancy","volume":"40","author":"Kramer","year":"2004","journal-title":"Eur. J. Cancer"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1007\/978-3-319-40389-2_3","article-title":"Lung Cancer Staging and Prognosis","volume":"170","author":"Woodard","year":"2016","journal-title":"Cancer Treat Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/j.soc.2016.02.004","article-title":"Mediastinal Staging in Non-Small Cell Lung Cancer","volume":"25","author":"Gamliel","year":"2016","journal-title":"Surg. Oncol. Clin. N. Am."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1007\/s12094-017-1739-6","article-title":"Validation of the AJCC 8th lung cancer staging system among patients with small cell lung cancer","volume":"20","year":"2018","journal-title":"Clin. Transl. Oncol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.jtcvs.2003.11.061","article-title":"Prolonged survival in patients with resected non-small cell lung cancer and single-level N2 disease","volume":"128","author":"Keller","year":"2004","journal-title":"J. Thorac. Cardiovasc.Surg."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1016\/S0022-5223(99)70217-5","article-title":"Prognosis of completely resected pN2 non-small cell lung carcinomas: What is the significant node that affecys survival?","volume":"118","author":"Okada","year":"1999","journal-title":"J. Thorac. Cardiovasc. Surg."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.lungcan.2004.10.002","article-title":"Endoscopic ultrasound guided biopsy versus mediastinoscopy for analysis of paratracheal and subcarinal lymph nodes in lung cancer staging","volume":"48","author":"Larsen","year":"2005","journal-title":"Lung Cancer"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.lungcan.2009.08.016","article-title":"EUS \u2013FNA in the preoperative staging of non-small cell lung cancer","volume":"69","author":"Talebian","year":"2010","journal-title":"Lung Cancer"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1097\/JTO.0b013e318287c0ce","article-title":"Comparison of endobronchial ultrasound and\/or endoesophageal ultrasound with transcervical extended mediastinal lymphadenectomy for staging and restaging of Non-Small Cell Lung Cancer","volume":"8","author":"Zielinski","year":"2013","journal-title":"J. Thorac. Oncol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"940","DOI":"10.1016\/j.athoracsur.2007.04.078","article-title":"Accessing the aortopulmonary window (#5) and the paraaortic (#6) lymph nodes in patients with Non-Small Cell Lung Cancer","volume":"84","author":"Cerfolio","year":"2007","journal-title":"Ann. Thorac. Surg."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1016\/j.athoracsur.2010.04.062","article-title":"The true false negative rates of esophageal and endobronchial ultrasound in the staging of mediastinal lymph nodes in patients with Non-Small Cell Lung Cancer","volume":"90","author":"Cerfolio","year":"2010","journal-title":"Ann. Thorac. Surg."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1097\/JTO.0b013e3181a0d82e","article-title":"The IASLC Lung Cancer Staging Project: A proposal for a new international lymph node map in the forthcoming seventh edition of the TNM classification for lung cancer","volume":"4","author":"Rusch","year":"2009","journal-title":"J. Thorac. Oncol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Roth, K., Eagan, T., and Hardie, J. (2016). Expert opinion of mediastinal lymph node positions from an intrabronchial view. BMC Pulm. Med., 16.","DOI":"10.1186\/s12890-016-0176-6"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.athoracsur.2017.03.074","article-title":"Endosonographic Mediastinal Lymph Node Staging in Non-Small Cell Lung Cancer: How I Teach It","volume":"104","author":"Oliveira","year":"2017","journal-title":"Ann. Thorac. Surg."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"S102","DOI":"10.1097\/JTO.0000000000000294","article-title":"Approaching the patient with an anterior mediastinal mass: A guide for clinicians","volume":"9","author":"Carter","year":"2014","journal-title":"J. Thorac. Oncol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1576","DOI":"10.1097\/JTO.0b013e3181c1274f","article-title":"Endoscopic and Endobronchial Ultrasonography According to the proposed lymph node map definition in the seventh edition of the tumor, node, metastasis classification for lung cancer","volume":"4","author":"Tournoy","year":"2009","journal-title":"J. Thorac. Oncol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"785425","DOI":"10.1155\/2011\/785425","article-title":"Endoscopic Ultrasound Fine Needle Aspiration in the Diagnosis of Lymphoma","volume":"2011","author":"Creemers","year":"2011","journal-title":"J. Oncol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1184","DOI":"10.1007\/BF00210924","article-title":"Video-assisted thoracoscopic management of anterior mediastinal masses. Preliminary experience and results","volume":"9","author":"Yim","year":"1995","journal-title":"Surg. Endosc."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1053\/crad.2002.1061","article-title":"CT-guided biopsy with cutting-edge needle for the diagnosis of malignant lymphoma: Experience of 267 biopsies","volume":"58","author":"Agid","year":"2003","journal-title":"Clin. Radiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1378\/chest.13-2339","article-title":"Endobronchial Ultrasound.Guided Transbronchia Needle Aspiration vs Conventional Transbronchial Needle Aspiration in The Diagnosis of Sarcoidosis","volume":"146","author":"Gupta","year":"2014","journal-title":"Chest"},{"key":"ref_48","first-page":"565","article-title":"The Diagnosis, Differential Diagnosis, and Treatment of Sarcoidosis","volume":"113","author":"Prasse","year":"2016","journal-title":"Dtsch. Arztebl. Int."},{"key":"ref_49","first-page":"840","article-title":"Diagnosis and Management of Sarcoidosis","volume":"93","author":"Peters","year":"2016","journal-title":"Am. Fam. Physician"}],"container-title":["Journal of Clinical Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2077-0383\/11\/18\/5469\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T00:33:20Z","timestamp":1760142800000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2077-0383\/11\/18\/5469"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,17]]},"references-count":49,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2022,9]]}},"alternative-id":["jcm11185469"],"URL":"https:\/\/doi.org\/10.3390\/jcm11185469","relation":{},"ISSN":["2077-0383"],"issn-type":[{"type":"electronic","value":"2077-0383"}],"subject":[],"published":{"date-parts":[[2022,9,17]]}}}