{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T11:02:51Z","timestamp":1781521371568,"version":"3.54.1"},"reference-count":39,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2022,10,19]],"date-time":"2022-10-19T00:00:00Z","timestamp":1666137600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002661","name":"Fonds de la Recherche Scientifique\u2014FNRS","doi-asserted-by":"publisher","award":["T.0171.21"],"award-info":[{"award-number":["T.0171.21"]}],"id":[{"id":"10.13039\/501100002661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002661","name":"Fonds de la Recherche Scientifique\u2014FNRS","doi-asserted-by":"publisher","award":["J.0143.22"],"award-info":[{"award-number":["J.0143.22"]}],"id":[{"id":"10.13039\/501100002661","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Foundation against Cancer (foundation of public interest, Belgium)","award":["T.0171.21"],"award-info":[{"award-number":["T.0171.21"]}]},{"name":"Foundation against Cancer (foundation of public interest, Belgium)","award":["J.0143.22"],"award-info":[{"award-number":["J.0143.22"]}]},{"name":"Fonds sp\u00e9ciaux de la Recherche (University of Li\u00e8ge)","award":["T.0171.21"],"award-info":[{"award-number":["T.0171.21"]}]},{"name":"Fonds sp\u00e9ciaux de la Recherche (University of Li\u00e8ge)","award":["J.0143.22"],"award-info":[{"award-number":["J.0143.22"]}]},{"name":"Centre Anticanc\u00e9reux pr\u00e8s l\u2019Universit\u00e9 de Li\u00e8ge","award":["T.0171.21"],"award-info":[{"award-number":["T.0171.21"]}]},{"name":"Centre Anticanc\u00e9reux pr\u00e8s l\u2019Universit\u00e9 de Li\u00e8ge","award":["J.0143.22"],"award-info":[{"award-number":["J.0143.22"]}]},{"name":"Fondation Hospitalo Universitaire L\u00e9on Fredericq (FHULF, University of Li\u00e8ge)","award":["T.0171.21"],"award-info":[{"award-number":["T.0171.21"]}]},{"name":"Fondation Hospitalo Universitaire L\u00e9on Fredericq (FHULF, University of Li\u00e8ge)","award":["J.0143.22"],"award-info":[{"award-number":["J.0143.22"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>BCL6 (B-cell lymphoma 6) is a proto-oncogene and transcriptional repressor initially described as being involved in B-cell lymphoma. Recently, this factor has been identified as a promising tissue biomarker which could be used to diagnose women affected by endometriosis. Previous studies used HSCORE for BCL6 staining quantification in the endometrium. However, this semi-quantitative technique of analysis has some limitations, including a lack of objectivity, robustness, and reproducibility that may lead to intra- and inter-observer variability. Our main goal was to develop an original computer-assisted method to quantify BCL6 staining from whole-slide images reliably. In order to test the efficiency of our new digital method of quantification, we compared endometrial BCL6 expression between fertile and infertile women without or with different stages of endometriosis by using the widely used HSCORE analysis and our new automatic digital image analysis. We find a higher expression of BCL6 in the endometrium of infertile women with endometriosis and women with stage IV endometriosis. Furthermore, we demonstrate a significant correlation between the two types of independent measurements, indicating the robustness of results and also the reliability of our computer-assisted method for BCL6 quantification. In conclusion, our work, by using this original computer-assisted method, enables BCL6 quantification more objectively, reliably, robustly, and promptly compared to HSCORE analysis.<\/jats:p>","DOI":"10.3390\/jcm11206164","type":"journal-article","created":{"date-parts":[[2022,10,19]],"date-time":"2022-10-19T22:19:53Z","timestamp":1666217993000},"page":"6164","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Comparison of Morphological and Digital-Assisted Analysis for BCL6 Endometrial Expression in Women with Endometriosis"],"prefix":"10.3390","volume":"11","author":[{"given":"Marlyne","family":"Squatrito","sequence":"first","affiliation":[{"name":"Laboratory of Tumor and Development Biology, GIGA-Cancer, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Blacher","sequence":"additional","affiliation":[{"name":"Laboratory of Tumor and Development Biology, GIGA-Cancer, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6162-1551","authenticated-orcid":false,"given":"Laurie","family":"Henry","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology Department, University of Liege, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soraya","family":"Labied","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology Department, University of Liege, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agn\u00e8s","family":"Noel","sequence":"additional","affiliation":[{"name":"Laboratory of Tumor and Development Biology, GIGA-Cancer, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Nisolle","sequence":"additional","affiliation":[{"name":"Laboratory of Tumor and Development Biology, GIGA-Cancer, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"},{"name":"Obstetrics and Gynecology Department, University of Liege, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4952-9637","authenticated-orcid":false,"given":"Carine","family":"Munaut","sequence":"additional","affiliation":[{"name":"Laboratory of Tumor and Development Biology, GIGA-Cancer, University of Li\u00e8ge, 4000 Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,10,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1234","DOI":"10.1177\/1933719116649711","article-title":"Endometrial BCL6 Overexpression in Eutopic Endometrium of Women with Endometriosis","volume":"23","author":"Lessey","year":"2016","journal-title":"Reprod. 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