{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T04:37:32Z","timestamp":1790224652975,"version":"4.1.0"},"reference-count":44,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2022,12,6]],"date-time":"2022-12-06T00:00:00Z","timestamp":1670284800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Introduction: The mitochondrial function of circulating peripheral blood mononuclear cells (PBMCs) is an interesting new approach to cardiac diseases. Thus, PBMC\u2019s mitochondrial respiration decreases in relation to heart failure severity. However, no data are available on heart-transplanted patients (Htx). Population and Methods: We determined PBMCs mitochondrial respiration by high-resolution respirometry (Oroboros Instruments) and superoxide anion production using electron paramagnetic resonance (Bruker-Biospin) in 20 healthy subjects and 20 matched Htx and investigated clinical, biological, echocardiographic, coronarography and biopsy characteristics. Results: PBMCs mitochondrial respiratory chain complex II respiration was decreased in Htx (4.69 \u00b1 0.84 vs. 7.69 \u00b1 1.00 pmol\/s\/million cell in controls and Htx patients, respectively; p = 0.007) and complex IV respiration was increased (24.58 \u00b1 2.57 vs. 15.68 \u00b1 1.67 pmol\/s\/million cell; p = 0.0035). Superoxide anion production was also increased in Htx (1.47 \u00b1 0.10 vs. 1.15 \u00b1 0.10 \u00b5mol\/min; p = 0.041). The leucocyte-to-lymphocyte ratio was increased in Htx, whom complex II correlated with leucocyte number (r = 0.51, p = 0.02) and with the left ventricular posterior wall peak early diastolic myocardial velocity (r = \u22120.62, p = 0.005). Complex IV was increased in the two patients with acute rejection and correlated negatively with Htx\u2019s isovolumetric relation time (r = \u22120.45, p = 0.045). Discussion: Although presenting with normal systolic function, Htx demonstrated abnormal PBMC\u2019s mitochondrial respiration. Unlike immunosuppressive therapies, subclinical diastolic dysfunction might be involved in these changes. Additionally, lymphopenia might reduce complex II, and acute rejection enhances complex IV respirations. Conclusion: PBMC\u2019s mitochondrial respiration appears modified in Htx, potentially linked to cellular shift, mild diastolic dysfunction and\/or acute rejection.<\/jats:p>","DOI":"10.3390\/jcm11237247","type":"journal-article","created":{"date-parts":[[2022,12,7]],"date-time":"2022-12-07T04:00:37Z","timestamp":1670385637000},"page":"7247","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Peripheral Blood Mononuclear Cells Mitochondrial Respiration and Superoxide Anion after Heart Transplantation"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5878-7737","authenticated-orcid":false,"given":"Abrar","family":"Alfatni","sequence":"first","affiliation":[{"name":"Team 3072 \u201cMitochondria, Oxidative Stress and Muscle Protection\u201d, Translational Medicine Federation of Strasbourg (FMTS), Faculty of Medicine, University of Strasbourg, 11 Rue Humann, 67000 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3557-2642","authenticated-orcid":false,"given":"Anne-Laure","family":"Charles","sequence":"additional","affiliation":[{"name":"Team 3072 \u201cMitochondria, Oxidative Stress and Muscle Protection\u201d, Translational Medicine Federation of Strasbourg (FMTS), Faculty of Medicine, University of Strasbourg, 11 Rue Humann, 67000 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Sauer","sequence":"additional","affiliation":[{"name":"Team 3072 \u201cMitochondria, Oxidative Stress and Muscle Protection\u201d, Translational Medicine Federation of Strasbourg (FMTS), Faculty of Medicine, University of Strasbourg, 11 Rue Humann, 67000 Strasbourg, France"},{"name":"Physiology and Functional Exploration Service, University Hospital of Strasbourg, NHC, 1 Place de l\u2019H\u00f4pital, CEDEX, 67091 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianne","family":"Riou","sequence":"additional","affiliation":[{"name":"Team 3072 \u201cMitochondria, Oxidative Stress and Muscle Protection\u201d, Translational Medicine Federation of Strasbourg (FMTS), Faculty of Medicine, University of Strasbourg, 11 Rue Humann, 67000 Strasbourg, France"},{"name":"Physiology and Functional Exploration Service, University Hospital of Strasbourg, NHC, 1 Place de l\u2019H\u00f4pital, CEDEX, 67091 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabienne","family":"Goupilleau","sequence":"additional","affiliation":[{"name":"Team 3072 \u201cMitochondria, Oxidative Stress and Muscle Protection\u201d, Translational Medicine Federation of Strasbourg (FMTS), Faculty of Medicine, University of Strasbourg, 11 Rue Humann, 67000 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2094-2842","authenticated-orcid":false,"given":"Samy","family":"Talha","sequence":"additional","affiliation":[{"name":"Team 3072 \u201cMitochondria, Oxidative Stress and Muscle Protection\u201d, Translational Medicine Federation of Strasbourg (FMTS), Faculty of Medicine, University of Strasbourg, 11 Rue Humann, 67000 Strasbourg, France"},{"name":"Physiology and Functional Exploration Service, University Hospital of Strasbourg, NHC, 1 Place de l\u2019H\u00f4pital, CEDEX, 67091 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Meyer","sequence":"additional","affiliation":[{"name":"Team 3072 \u201cMitochondria, Oxidative Stress and Muscle Protection\u201d, Translational Medicine Federation of Strasbourg (FMTS), Faculty of Medicine, University of Strasbourg, 11 Rue Humann, 67000 Strasbourg, France"},{"name":"Physiology and Functional Exploration Service, University Hospital of Strasbourg, NHC, 1 Place de l\u2019H\u00f4pital, CEDEX, 67091 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7914-7616","authenticated-orcid":false,"given":"Emmanuel","family":"Andres","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, University Hospital of Strasbourg, 1 Place de l\u2019H\u00f4pital, CEDEX, 67091 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8884-4395","authenticated-orcid":false,"given":"Michel","family":"Kindo","sequence":"additional","affiliation":[{"name":"Cardiovascular Service, University Hospital of Strasbourg, NHC, 1 Place de l\u2019H\u00f4pital, CEDEX, 67091 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Philippe","family":"Mazzucotelli","sequence":"additional","affiliation":[{"name":"Cardiovascular Service, University Hospital of Strasbourg, NHC, 1 Place de l\u2019H\u00f4pital, CEDEX, 67091 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4774-730X","authenticated-orcid":false,"given":"Eric","family":"Epailly","sequence":"additional","affiliation":[{"name":"Cardiovascular Service, University Hospital of Strasbourg, NHC, 1 Place de l\u2019H\u00f4pital, CEDEX, 67091 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard","family":"Geny","sequence":"additional","affiliation":[{"name":"Team 3072 \u201cMitochondria, Oxidative Stress and Muscle Protection\u201d, Translational Medicine Federation of Strasbourg (FMTS), Faculty of Medicine, University of Strasbourg, 11 Rue Humann, 67000 Strasbourg, France"},{"name":"Physiology and Functional Exploration Service, University Hospital of Strasbourg, NHC, 1 Place de l\u2019H\u00f4pital, CEDEX, 67091 Strasbourg, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1038\/nrcardio.2016.203","article-title":"Mitochondrial Function as a Therapeutic Target in Heart Failure","volume":"14","author":"Brown","year":"2017","journal-title":"Nat. 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