{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:50:31Z","timestamp":1787028631925,"version":"build-2736575974"},"reference-count":46,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,1,19]],"date-time":"2023-01-19T00:00:00Z","timestamp":1674086400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82122017"],"award-info":[{"award-number":["82122017"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81870642"],"award-info":[{"award-number":["81870642"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970780"],"award-info":[{"award-number":["81970780"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670835"],"award-info":[{"award-number":["81670835"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["shslczdzk01901"],"award-info":[{"award-number":["shslczdzk01901"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shanghai Municipal Key Clinical Specialty Program","award":["82122017"],"award-info":[{"award-number":["82122017"]}]},{"name":"Shanghai Municipal Key Clinical Specialty Program","award":["81870642"],"award-info":[{"award-number":["81870642"]}]},{"name":"Shanghai Municipal Key Clinical Specialty Program","award":["81970780"],"award-info":[{"award-number":["81970780"]}]},{"name":"Shanghai Municipal Key Clinical Specialty Program","award":["81670835"],"award-info":[{"award-number":["81670835"]}]},{"name":"Shanghai Municipal Key Clinical Specialty Program","award":["shslczdzk01901"],"award-info":[{"award-number":["shslczdzk01901"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>High-myopic cataract (HMC) is a complex cataract with earlier onset and more rapid progress than age-related cataract (ARC). Circular RNAs (circRNAs) have been implicated in many diseases. However, their involvement in HMC remain largely unexplored. To investigate the role of dysregulated circRNAs in HMC, lens epithelium samples from 24 HMC and 24 ARC patients were used for whole transcriptome sequencing. Compared with ARC, HMC had 3687 uniquely expressed circRNAs and 1163 significantly differentially expressed circRNAs (DEcRs) (|log2FC| &gt; 1, p &lt; 0.05). A putative circRNA-miRNA-mRNA network was constructed based on correlation analysis. We validated the differential expression of 3 DEcRs by quantitative polymerase chain reaction (qPCR) using different sets of samples. We further investigated the role of circAFF1 in cultured lens epithelial cells (LECs) and found that the overexpression of circAFF1 promoted cell proliferation, migration and inhibited apoptosis. We also showed that circAFF1 upregulated Tropomyosin 1 (TPM1) expression by sponging miR-760, which was consistent with the network prediction. Collectively, our study suggested the involvement of circRNAs in the pathogenesis of HMC and provide a resource for further study on this topic.<\/jats:p>","DOI":"10.3390\/jcm12030813","type":"journal-article","created":{"date-parts":[[2023,1,19]],"date-time":"2023-01-19T07:57:38Z","timestamp":1674115058000},"page":"813","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The Differential Expression of Circular RNAs and the Role of circAFF1 in Lens Epithelial Cells of High-Myopic Cataract"],"prefix":"10.3390","volume":"12","author":[{"given":"Shiyu","family":"Ma","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China"},{"name":"Eye Institute, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China"},{"name":"Key Laboratory of Myopia, Ministry of Health PR China, Shanghai 200031, China"},{"name":"Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai Medical College, Fudan University, Shanghai 200031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangjia","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China"},{"name":"Eye Institute, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China"},{"name":"Key Laboratory of Myopia, Ministry of Health PR China, Shanghai 200031, China"},{"name":"Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai Medical College, Fudan University, Shanghai 200031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China"},{"name":"Eye Institute, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China"},{"name":"Key Laboratory of Myopia, Ministry of Health PR China, Shanghai 200031, China"},{"name":"Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai Medical College, Fudan University, Shanghai 200031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China"},{"name":"Eye Institute, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China"},{"name":"Key Laboratory of Myopia, Ministry of Health PR China, Shanghai 200031, China"},{"name":"Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai Medical College, Fudan University, Shanghai 200031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqi","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China"},{"name":"Eye Institute, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China"},{"name":"Key Laboratory of Myopia, Ministry of Health PR China, Shanghai 200031, China"},{"name":"Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai Medical College, Fudan University, Shanghai 200031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9770-5733","authenticated-orcid":false,"given":"Yongxiang","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China"},{"name":"Eye Institute, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China"},{"name":"Key Laboratory of Myopia, Ministry of Health PR China, Shanghai 200031, China"},{"name":"Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai Medical College, Fudan University, Shanghai 200031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Facial Plastic and Reconstructive Surgery, ENT Institute, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China"},{"name":"Eye Institute, Eye and ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China"},{"name":"Key Laboratory of Myopia, Ministry of Health PR China, Shanghai 200031, China"},{"name":"Shanghai Key Laboratory of Visual Impairment and Restoration, Shanghai Medical College, Fudan University, Shanghai 200031, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,19]]},"reference":[{"key":"ref_1","first-page":"3625","article-title":"Myopia and incident cataract and cataract surgery: The blue mountains eye study","volume":"43","author":"Younan","year":"2002","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"ref_2","first-page":"1449","article-title":"Refractive errors and incident cataracts: The beaver dam eye study","volume":"42","author":"Wong","year":"2001","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1111\/ceo.12206","article-title":"Myopia and the long-term incidence of cataract and cataract surgery: The blue mountains eye study","volume":"42","author":"Kanthan","year":"2014","journal-title":"Clin. Exp. Ophthalmol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.1016\/j.ophtha.2016.01.006","article-title":"Global prevalence of myopia and high myopia and temporal trends from 2000 through 2050","volume":"123","author":"Holden","year":"2016","journal-title":"Ophthalmology"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1097\/ICU.0000000000000217","article-title":"High myopia and cataract surgery","volume":"27","author":"Chong","year":"2016","journal-title":"Curr. Opin. Ophthalmol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1473","DOI":"10.1097\/j.jcrs.0000000000000664","article-title":"Efficacy and complications of cataract surgery in high myopia","volume":"47","author":"Yao","year":"2021","journal-title":"J. Cataract. Refract. Surg."},{"key":"ref_7","first-page":"2347","article-title":"Overview of the complications of high myopia","volume":"37","author":"Ikuno","year":"2017","journal-title":"Retin.-J. Retin. Vitr. Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1016\/j.ajhg.2011.08.003","article-title":"High myopia caused by a mutation in leprel1, encoding prolyl 3-hydroxylase 2","volume":"89","author":"Mordechai","year":"2011","journal-title":"Am. J. Hum. Genet."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1111\/cge.12309","article-title":"Homozygous loss-of-function mutation of the leprel1 gene causes severe non-syndromic high myopia with early-onset cataract","volume":"86","author":"Guo","year":"2014","journal-title":"Clin. Genet."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Yang, J., Zhou, S., Gu, J., Guo, M., Xia, H., and Liu, Y. (2015). Upr activation and the down-regulation of \u03b1-crystallin in human high myopia-related cataract lens epithelium. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0137582"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.molmed.2012.03.005","article-title":"Protein misfolding and aggregation in cataract disease and prospects for prevention","volume":"18","author":"Moreau","year":"2012","journal-title":"Trends Mol. Med"},{"key":"ref_12","first-page":"1","article-title":"Elevated transforming growth factor-\u03b22 in the aqueous humor: A possible explanation for high rate of capsular contraction syndrome in high myopia","volume":"2016","author":"Zhang","year":"2016","journal-title":"J. Ophthalmol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.exer.2015.03.017","article-title":"Proinflammatory status in the aqueous humor of high myopic cataract eyes","volume":"142","author":"Zhu","year":"2016","journal-title":"Exp. Eye Res."},{"key":"ref_14","first-page":"150","article-title":"The expression of cytokines in aqueous humor of high myopic patients with cataracts","volume":"26","author":"Zhang","year":"2020","journal-title":"Mol. Vis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1080\/02713683.2016.1223317","article-title":"Correlations between mmps and timps levels in aqueous humor from high myopia and cataract patients","volume":"42","author":"Jia","year":"2017","journal-title":"Curr. Eye Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1038\/nature11928","article-title":"Circular rnas are a large class of animal rnas with regulatory potency","volume":"495","author":"Memczak","year":"2013","journal-title":"Nature"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1016\/j.ebiom.2019.10.004","article-title":"Downregulation of circrna dmnt3b contributes to diabetic retinal vascular dysfunction through targeting mir-20b-5p and bambi","volume":"49","author":"Zhu","year":"2019","journal-title":"Ebiomedicine"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"771552","DOI":"10.3389\/fendo.2021.771552","article-title":"Mirna, lncrna and circrna: Targeted molecules full of therapeutic prospects in the development of diabetic retinopathy","volume":"12","author":"Chang","year":"2021","journal-title":"Front. Endocrinol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3293","DOI":"10.7150\/thno.39488","article-title":"Circular rna-zbtb44 regulates the development of choroidal neovascularization","volume":"10","author":"Zhou","year":"2020","journal-title":"Theranostics"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1080\/02713683.2020.1722179","article-title":"Investigation of circrna expression profiles and analysis of circrna-mirna-mrna networks in an animal (mouse) model of age-related macular degeneration","volume":"45","author":"Liu","year":"2020","journal-title":"Curr. Eye Res."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"556712","DOI":"10.3389\/fgene.2020.556712","article-title":"Circular rna expression profiling identifies glaucoma-related circular rnas in various chronic ocular hypertension rat models","volume":"11","author":"Chen","year":"2020","journal-title":"Front. Genet."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1757","DOI":"10.1002\/cbin.11615","article-title":"Circular rna circznf292 regulates h2o2 -induced injury in human lens epithelial hle-b3 cells depending on the regulation of the mir-222-3p\/e2f3 axis","volume":"45","author":"Xu","year":"2021","journal-title":"Cell Biol. Int."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"108128","DOI":"10.1016\/j.exer.2020.108128","article-title":"Circular rna hipk3 regulates human lens epithelial cell dysfunction by targeting the mir-221\u20133p\/pi3k\/akt pathway in age-related cataract","volume":"198","author":"Cui","year":"2020","journal-title":"Exp. Eye Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1016\/S0886-3350(02)01839-4","article-title":"Clinical application of the lens opacities classification system iii in the performance of phacoemulsification","volume":"29","author":"Davison","year":"2003","journal-title":"J. Cataract. Refract. Surg."},{"key":"ref_25","first-page":"3485","article-title":"Mir-34 promotes apoptosis of lens epithelial cells in cataract rats via the tgf-\u03b2\/smads signaling pathway","volume":"24","author":"Zhang","year":"2020","journal-title":"Eur. Rev. Med. Pharmacol. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Antosova, B., Smolikova, J., Borkovcova, R., Strnad, H., Lachova, J., Machon, O., and Kozmik, Z. (2013). Ectopic activation of wnt\/\u03b2-catenin signaling in lens fiber cells results in cataract formation and aberrant fiber cell differentiation. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0078279"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"587","DOI":"10.2174\/1874467213666200908120501","article-title":"Quercetin inhibits aqp1 translocation in high-glucose-cultured sra01\/04 cells through pi3k\/akt\/mtor pathway","volume":"14","author":"He","year":"2020","journal-title":"Curr. Molec. Pharmacol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"850","DOI":"10.3389\/fcell.2020.00850","article-title":"Circrna is a rising star in researches of ocular diseases","volume":"8","author":"Zhang","year":"2020","journal-title":"Front. Cell Dev. Biol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"5383","DOI":"10.18632\/aging.202470","article-title":"Circmre11a_013 binds to ubxn1 and integrates atm activation enhancing lens epithelial cells senescence in age-related cataract","volume":"13","author":"Liu","year":"2021","journal-title":"Aging"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"17271","DOI":"10.18632\/aging.103683","article-title":"Profiling of circular rnas in age-related cataract reveals circznf292 as an antioxidant by sponging mir-23b-3p","volume":"12","author":"Liang","year":"2020","journal-title":"Aging"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"8953","DOI":"10.1080\/21655979.2021.1990196","article-title":"Circular rna erythrocyte membrane protein band 4.1 assuages ultraviolet irradiation-induced apoptosis of lens epithelial cells by stimulating 5\u2019-bisphosphate nucleotidase 1 in a mir-24-3p-dependent manner","volume":"12","author":"Zhou","year":"2021","journal-title":"Bioengineered"},{"key":"ref_32","first-page":"1","article-title":"Circ_0122396 protects human lens epithelial cells from hydrogen peroxide-induced injury by binding to mir-15a-5p to stimulate fgf1 expression","volume":"47","author":"He","year":"2021","journal-title":"Curr. Eye Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1016\/j.gene.2019.05.054","article-title":"Circular rna circ kmt2e is up-regulated in diabetic cataract lenses and is associated with mir-204-5p sponge function","volume":"710","author":"Fan","year":"2019","journal-title":"Gene"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1822","DOI":"10.1080\/02713683.2021.1933058","article-title":"Circpag1 inhibits the high glucose-induced lens epithelial cell injury by sponging mir-630 and upregulating epha2","volume":"46","author":"Yang","year":"2021","journal-title":"Curr. Eye Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/15384101.2021.2018213","article-title":"Circpag1 interacts with mir-211-5p to promote the e2f3 expression and inhibit the high glucose-induced cell apoptosis and oxidative stress in diabetic cataract","volume":"21","author":"Tao","year":"2022","journal-title":"Cell Cycle"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1167\/iovs.61.10.13","article-title":"Identification and characterization of n6-methyladenosine circrnas and methyltransferases in the lens epithelium cells from age-related cataract","volume":"61","author":"Li","year":"2020","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2016","DOI":"10.1016\/j.cell.2022.04.021","article-title":"Circular rnas: Characterization, cellular roles, and applications","volume":"185","author":"Liu","year":"2022","journal-title":"Cell"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1795","DOI":"10.1007\/s11427-021-1993-6","article-title":"Linking circular intronic rna degradation and function in transcription by rnase h1","volume":"64","author":"Li","year":"2021","journal-title":"Sci. China-Life Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.molcel.2021.11.032","article-title":"Circular rna znf609\/ckap5 mrna interaction regulates microtubule dynamics and tumorigenicity","volume":"82","author":"Rossi","year":"2022","journal-title":"Mol. Cell"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2846","DOI":"10.1093\/nar\/gkw027","article-title":"Foxo3 circular rna retards cell cycle progression via forming ternary complexes with p21 and cdk2","volume":"44","author":"Du","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"4183","DOI":"10.7150\/thno.21299","article-title":"Identifying and characterizing circrna-protein interaction","volume":"7","author":"Du","year":"2017","journal-title":"Theranostics"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.exer.2016.03.009","article-title":"The cause and consequence of fiber cell compaction in the vertebrate lens","volume":"156","author":"Bassnett","year":"2017","journal-title":"Exp. Eye Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/j.jds.2021.11.003","article-title":"Knockdown of lncrna neat1 inhibits myofibroblast activity in oral submucous fibrosis through mir-760\/tpm1 axis","volume":"17","author":"Li","year":"2022","journal-title":"J. Dental Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1111\/j.1582-4934.2012.01654.x","article-title":"Elevated tropomyosin expression is associated with epithelial-mesenchymal transition of lens epithelial cells","volume":"17","author":"Kubo","year":"2013","journal-title":"J. Cell. Mol. Med."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"9880","DOI":"10.1021\/bi301323n","article-title":"A-tropomyosin with a d175n or e180g mutation in only one chain differs from tropomyosin with mutations in both chains","volume":"51","author":"Janco","year":"2012","journal-title":"Biochemistry"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Shibata, S., Shibata, N., Ohtsuka, S., Yoshitomi, Y., Kiyokawa, E., Yonekura, H., Singh, D.P., Sasaki, H., and Kubo, E. (2021). Role of decorin in posterior capsule opacification and eye lens development. Cells, 10.","DOI":"10.3390\/cells10040863"}],"container-title":["Journal of Clinical Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2077-0383\/12\/3\/813\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:10:48Z","timestamp":1760119848000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2077-0383\/12\/3\/813"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,19]]},"references-count":46,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["jcm12030813"],"URL":"https:\/\/doi.org\/10.3390\/jcm12030813","relation":{},"ISSN":["2077-0383"],"issn-type":[{"value":"2077-0383","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,19]]}}}