{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T14:28:43Z","timestamp":1781015323727,"version":"3.54.1"},"reference-count":74,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,2,9]],"date-time":"2023-02-09T00:00:00Z","timestamp":1675900800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100014269","name":"Medical University of Bialystok","doi-asserted-by":"publisher","award":["133-49616L"],"award-info":[{"award-number":["133-49616L"]}],"id":[{"id":"10.13039\/501100014269","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Studies have shown that osteopontin (OPN) and regulatory T cells play a role in allergic contact dermatitis (ACD), but the mechanisms responsible for their function are poorly understood. The study aimed to determine CD4 T lymphocytes producing intracellular osteopontin (iOPN T cells) and assess the selected T lymphocyte subsets including regulatory T cells in the blood of patients with ACD. Twenty-six patients with a disseminated form of allergic contact dermatitis and 21 healthy controls were enrolled in the study. Blood samples were taken twice: in the acute phase of the disease and during remission. The samples were analyzed by the flow cytometry method. Patients with acute ACD showed significantly higher percentage of iOPN T cells compared with healthy controls which persisted during remission. An increase in the percentage of CD4CD25 and a reduced percentage of regulatory T lymphocytes (CD4CD25highCD127low) were also found in the patients with acute stage of ACD. The percentage of CD4CD25 T lymphocytes showed a positive correlation with the EASI index. The increase in the iOPN T cells can indicate their participation in acute ACD. The decreased percentage of regulatory T lymphocytes in the acute stage of ACD may be related to the transformation of Tregs into CD4CD25 T cells. It may also indicate their increased recruitment to the skin. The positive correlation between the percentage of CD4CD25 lymphocytes and the EASI index may be indirect evidence for the importance of activated lymphocytes\u2014CD4CD25 in addition to CD8 lymphocytes as effector cells in ACD.<\/jats:p>","DOI":"10.3390\/jcm12041397","type":"journal-article","created":{"date-parts":[[2023,2,10]],"date-time":"2023-02-10T01:35:29Z","timestamp":1675992929000},"page":"1397","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Osteopontin and Regulatory T Cells in Effector Phase of Allergic Contact Dermatitis"],"prefix":"10.3390","volume":"12","author":[{"given":"Teresa","family":"Reduta","sequence":"first","affiliation":[{"name":"Department of Dermatology and Venereology, Medical University of Bialystok, Zurawia 14 St., 15-540 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Bacharewicz-Szczerbicka","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology, Medical University of Bialystok, Zurawia 14 St., 15-540 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Stasiak-Barmuta","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, Medical University of Bialystok, Waszyngtona 17 St., 15-274 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8688-4171","authenticated-orcid":false,"given":"Tomasz W.","family":"Kaminski","sequence":"additional","affiliation":[{"name":"Pittsburgh Heart, Lung and Blood Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, PA 15260, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iwona","family":"Flisiak","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology, Medical University of Bialystok, Zurawia 14 St., 15-540 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1007\/s00018-011-0846-8","article-title":"Allergic contact dermatitis: Epidemiology, molecular mechanisms, in vitro methods and regulatory aspects","volume":"69","author":"Peiser","year":"2012","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1111\/cod.13079","article-title":"Contact allergy in Danish children: Current trends","volume":"79","author":"Simonsen","year":"2018","journal-title":"Contact Dermat."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1146\/annurev.immunol.25.022106.141557","article-title":"IL-17 Family Cytokines and the Expanding Diversity of Effector T Cell Lineages","volume":"25","author":"Weaver","year":"2007","journal-title":"Annu. Rev. Immunol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1038\/jid.2012.284","article-title":"Update of Immune Events in the Murine Contact Hypersensitivity Model: Toward the Understanding of Allergic Contact Dermatitis","volume":"133","author":"Honda","year":"2013","journal-title":"J. Investig. Dermatol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1097\/CRD.0b013e3181cfb646","article-title":"Osteopontin in cardiovascular disease: A potential therapeutic target","volume":"18","author":"Waller","year":"2010","journal-title":"Cardiol. Rev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2302","DOI":"10.1161\/ATVBAHA.107.144824","article-title":"Osteopontin: A multifunctional molecule regulating chronic inflammation and vascular disease","volume":"27","author":"Scatena","year":"2007","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"12713","DOI":"10.1038\/srep12713","article-title":"The impact of osteopontin on prognosis and clinicopathology of colorectal cancer patients: A systematic meta-analysis","volume":"5","author":"Zhao","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1111\/j.1440-1827.2011.02649.x","article-title":"Osteopontin, intrinsic tissue regulator of intractable inflammatory diseases","volume":"61","author":"Uede","year":"2011","journal-title":"Pathol. Int."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"750","DOI":"10.1111\/j.1600-0625.2009.00926.x","article-title":"Osteopontin and the skin: Multiple emerging roles in cutaneous biology and pathology","volume":"18","author":"Buback","year":"2009","journal-title":"Exp. Dermatol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"7235","DOI":"10.1073\/pnas.0802301105","article-title":"Alternative translation of osteopontin generates intracellular and secreted isoforms that mediate distinct biological activities in dendritic cells","volume":"105","author":"Shinohara","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1016\/j.immuni.2008.05.008","article-title":"Engagement of the type I interferon receptor on dendritic cells inhibits T helper 17 cell development: Role of intracellular osteopontin","volume":"29","author":"Shinohara","year":"2008","journal-title":"Immunity"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1038\/nri2460","article-title":"Regulation of T-helper-cell lineage development by osteopontin: The inside story","volume":"9","author":"Cantor","year":"2009","journal-title":"Nat. Rev. Immunol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/j.cytogfr.2008.08.001","article-title":"Osteopontin: Role in immune regulation and stress responses","volume":"19","author":"Wang","year":"2008","journal-title":"Cytokine Growth Factor Rev."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Bartosi\u0144ska, J., Przepi\u00f3rka-Kosi\u0144ska, J., Sarecka-Hujar, B., Raczkiewicz, D., Kowal, M., Chyl-Surdacka, K., Bartosi\u0144ski, J., Kosi\u0144ski, J., Krasowska, D., and Chodorowska, G. (2021). Osteopontin Serum Concentration and Metabolic Syndrome in Male Psoriatic Patients. J. Clin. Med., 10.","DOI":"10.3390\/jcm10040755"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1007\/s00403-009-0939-5","article-title":"Osteopontin: A new emerging role in psoriasis","volume":"301","author":"Buommino","year":"2009","journal-title":"Arch. Dermatol. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/j.jaad.2008.09.046","article-title":"Elevated plasma osteopontin level is associated with occurrence of psoriasis and is an unfavorable cardiovascular risk factor in patients with psoriasis","volume":"60","author":"Chen","year":"2009","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Lavi, H., Assayag, M., Schwartz, A., Arish, N., Fridlender, Z.G., and Berkman, N. (2017). The association between osteopontin gene polymorphisms, osteopontin expression and sarcoidosis. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0171945"},{"key":"ref_18","first-page":"43","article-title":"The influence of osteopontin on the pathogenesis of alopecia areata and its association with disease severity","volume":"21","author":"Soheila","year":"2018","journal-title":"Iran. J. Dermatol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.aller.2013.01.009","article-title":"Elevated level of serum osteopontin in school-age children with asthma","volume":"42","author":"Akelma","year":"2014","journal-title":"Allergol. Immunopathol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2590","DOI":"10.1172\/JCI58978","article-title":"Periostin promotes chronic allergic inflammation in response to Th2 cytokines","volume":"122","author":"Masuoka","year":"2012","journal-title":"J. Clin. Investig."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"48","DOI":"10.15761\/GVI.1000114","article-title":"The role of osteopontin in skin diseases","volume":"1","author":"Bassyouni","year":"2016","journal-title":"Glob. Vaccines Immunol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"246","DOI":"10.2353\/ajpath.2010.090488","article-title":"Antigen-specific induction of osteopontin contributes to the chronification of allergic contact dermatitis","volume":"176","author":"Seier","year":"2010","journal-title":"Am. J. Pathol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1219","DOI":"10.1084\/jem.194.9.1219","article-title":"Osteopontin Is Involved in the Initiation of Cutaneous Contact Hypersensitivity by Inducing Langerhans and Dendritic Cell Migration to Lymph Nodes","volume":"194","author":"Weiss","year":"2001","journal-title":"J. Exp. Med."},{"key":"ref_24","first-page":"206","article-title":"Differentiation, maturation, and survival of dendritic cells by osteopontin regulation","volume":"12","author":"Kawamura","year":"2005","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.advms.2015.05.001","article-title":"Serum osteopontin levels in disseminated allergic contact dermatitis","volume":"60","author":"Reduta","year":"2015","journal-title":"Adv. Med. Sci."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1151","DOI":"10.4049\/jimmunol.155.3.1151","article-title":"Immunologic self-tolerance maintained by activated T cells expressing IL-2 receptor \u03b1-chains (CD25). Breakdown of a single mechanism of self-tolerance causes various autoimmune diseases","volume":"155","author":"Sakaguchi","year":"1995","journal-title":"J. Immunol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1126\/science.1079490","article-title":"Control of regulatory cell development by the transcription factor Fox3","volume":"299","author":"Hori","year":"2003","journal-title":"Science"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"3262","DOI":"10.4049\/jimmunol.171.6.3262","article-title":"Cytokine Milieu of Atopic Dermatitis, as Compared to Psoriasis, Skin Prevents Induction of Innate Immune Response Genes","volume":"171","author":"Nomura","year":"2003","journal-title":"J. Immunol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1038\/ni1178","article-title":"Naturally arising Foxp3-expressing CD25+CD4+ regulatory T cells in immunological tolerance to self and not-self","volume":"6","author":"Sakagouchi","year":"2005","journal-title":"Nat. Immunol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2736","DOI":"10.1182\/blood.V98.9.2736","article-title":"Human CD4+CD25+ cells: A naturally occurring population of regulatory T cells","volume":"98","author":"Ng","year":"2001","journal-title":"Blood"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.4049\/jimmunol.167.3.1245","article-title":"CD4+CD25high Regulatory Cells in Human Peripheral Blood","volume":"167","author":"Brown","year":"2001","journal-title":"J. Immunol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1146\/annurev.immunol.24.021605.090547","article-title":"FOXP3: Of mice and men","volume":"24","author":"Ziegler","year":"2006","journal-title":"Annu. Rev. Immunol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1701","DOI":"10.1084\/jem.20060772","article-title":"CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4 Treg cells","volume":"203","author":"Liu","year":"2006","journal-title":"J. Exp. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1111\/j.1365-2249.2011.04334.x","article-title":"Interleukin-7 matures suppressive CD127(+) forkhead box P3 (FoxP3)(+) T cells into CD127(-) CD25(high) FoxP3(+) regulatory T cells","volume":"165","author":"Phillips","year":"2011","journal-title":"Clin. Exp. Immunol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1172\/JCI72932","article-title":"Memory regulatory T cells reside in human skin","volume":"124","author":"Rodriguez","year":"2014","journal-title":"J. Clin. Investig."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1016\/j.immuni.2015.10.016","article-title":"A vawe of regulatory T cells into neonatal skin mediates tolerance to commensal microbes","volume":"43","author":"Scharschmidt","year":"2015","journal-title":"Immunity"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3049","DOI":"10.4049\/jimmunol.1203205","article-title":"Dermal regulatory T cells display distinct migratory behavior that is modulated during adaptive and innate inflammation","volume":"191","author":"Chow","year":"2013","journal-title":"J. Immunol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"4483","DOI":"10.4049\/jimmunol.1300212","article-title":"Memory regulatory t cells require IL-7 and not IL-2 for their maintenance in peripheral tissues","volume":"190","author":"Gratz","year":"2013","journal-title":"J. Immunol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1016\/j.chom.2017.03.001","article-title":"Commensal Microbes and Hair Follicle Morphogenesis Coordinately Drive Treg Migration into Neonatal Skin","volume":"21","author":"Scharschmidt","year":"2017","journal-title":"Cell Host Microbe"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1111\/j.0105-2896.2006.00427.x","article-title":"Foxp3+ CD25+ CD4+ natural regulatory T cells in dominant self-tolerance and autoimmune disease","volume":"212","author":"Sakaguchi","year":"2006","journal-title":"Immunol. Rev."},{"key":"ref_41","first-page":"338","article-title":"Mechanisms of suppression by suppressor T cells","volume":"5","year":"2005","journal-title":"Nat. Immunol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1016\/j.autrev.2008.03.001","article-title":"Mechanisms of T regulatory cell function","volume":"7","author":"Askenasy","year":"2008","journal-title":"Autoimmun. Rev."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1034\/j.1600-0625.2001.100102.x","article-title":"The Easi Evaluator Group The eczema area and severity index (EASI): Assessment of reliability in atopic dermatitis","volume":"10","author":"Hanifin","year":"2001","journal-title":"Exp. Dermatol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1002\/jcp.10394","article-title":"Osteopontin modulates CD44-dependent chemotaxis of peritoneal macrophages through G-protein-coupled receptors: Evidence of a role for an intracellular form of osteopontin","volume":"198","author":"Zhu","year":"2004","journal-title":"J. Cell. Physiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1002\/(SICI)1097-4652(200007)184:1<118::AID-JCP13>3.0.CO;2-Y","article-title":"Intracellular osteopontin is an integral component of the CD44-ERM complex involved in cell migration","volume":"184","author":"Zohar","year":"2000","journal-title":"J. Cell. Physiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1254\/jphs.FMJ05004X2","article-title":"Current topics in pharmacological research on bone metabolism: Inhibitory effects of bisphosphonates on the differentiation and activity of osteoclasts","volume":"100","author":"Suzuki","year":"2006","journal-title":"J. Pharmacol. Sci."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1152\/ajpcell.00477.2006","article-title":"Osteopontin localizes to the nucleus of 293 cells and associates with polo-like kinase-1","volume":"292","author":"Junaid","year":"2007","journal-title":"Am. J. Physiol. Cell. Physiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1007\/s12026-010-8179-5","article-title":"Intracellular osteopontin (iOPN) and immunity","volume":"49","author":"Inoue","year":"2011","journal-title":"Immunol. Res."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1073\/pnas.1423011112","article-title":"Intracellular osteopontin regulates homeostasis and function of natural killer cells","volume":"112","author":"Leavenworth","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1038\/ni1327","article-title":"Osteopontin expression is essential for interferon-alpha production by plasmacytoid dendritic cells","volume":"7","author":"Shinohara","year":"2006","journal-title":"Nat. Immunol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"17101","DOI":"10.1073\/pnas.0508666102","article-title":"T-bet-dependent expression of osteopontin contributes to T cell polarization","volume":"102","author":"Shinohara","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1159\/000442749","article-title":"Serum Biomarkers of Allergic Contact Dermatitis: A Pilot Study","volume":"168","author":"Kainov","year":"2015","journal-title":"Int. Arch. Allergy Immunol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1084\/jem.189.5.779","article-title":"Cytotoxicity is mandatory for CD8(+) T cell-mediated contact hypersensitivity","volume":"189","author":"Kehren","year":"1999","journal-title":"J. Exp. Med."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1111\/j.1346-8138.2002.tb00206.x","article-title":"Initaial recruitment of interferon-gamma-producing CD8+ effector cells, followed by infiltration of CD4+ cells in 2,4,6-trinitro-1-chlorobenzene (TNCB)-induced murine contact hypersensitivity reactions","volume":"29","author":"Okazaki","year":"2002","journal-title":"J. Dermatol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1007\/3-540-37673-9_12","article-title":"Regulatory T cells in psoriasis","volume":"56","author":"Kagen","year":"2006","journal-title":"Ernst. Schering. Res. Found. Workshop"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1785","DOI":"10.1038\/jid.2011.200","article-title":"The Dark Side of Regulatory T Cells in Psoriasis","volume":"131","author":"Soler","year":"2011","journal-title":"J. Investig. Dermatol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.jdermsci.2015.11.007","article-title":"Impaired function of regulatory T cells in patients with psoriasis is mediated by phosphorylation of STAT3","volume":"81","author":"Yang","year":"2016","journal-title":"J. Dermatol. Sci."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.jdermsci.2013.01.006","article-title":"Impaired inhibitory function of circulating CD4+CD25+ regulatory T cells in alopecia areata","volume":"70","author":"Shin","year":"2013","journal-title":"J. Dermatol. Sci."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1111\/bjd.12396","article-title":"T-helper and regulatory T-cell cytokines in the peripheral blood of patients with active alopecia areata","volume":"169","author":"Tembhre","year":"2013","journal-title":"Br. J. Dermatol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"e27","DOI":"10.1016\/j.jdermsci.2017.02.080","article-title":"Activated regulatory T cells are increased in patients with alopecia areata for suppressing disease acitivity","volume":"86","author":"Kubo","year":"2017","journal-title":"J. Dermatol. Sci."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1016\/j.jaci.2004.01.772","article-title":"T regulatory cells in atopic dermatitis and subversion of their activity by superantigens","volume":"113","author":"Ou","year":"2004","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1016\/j.jaci.2007.11.003","article-title":"Analysis of CD25hiCD4+ \u201cregulatory\u201d T-cell subtypes in atopic dermatitis reveals a novel T(H)2-like population","volume":"121","author":"Reefer","year":"2008","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1159\/000323305","article-title":"The Role of Regulatory T Cells in Atopic Dermatitis","volume":"41","author":"Agrawal","year":"2011","journal-title":"Curr. Probl. Dermatol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"984","DOI":"10.1111\/j.1365-2133.2009.09035.x","article-title":"Regulatory T cells in atopic dermatitis: Epidermal dendritic cell clusters may contribute to their local expansion","volume":"160","author":"Szegedi","year":"2009","journal-title":"Br. J. Dermatol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1046\/j.1523-1747.1998.00334.x","article-title":"Patients with allergic contact dermatitis to nickel and nonallergic individuals display different nickel-specific T cell responses. Evidence for the presence of effector CD8+ and regulatory CD4+ T cells","volume":"111","author":"Cavani","year":"1998","journal-title":"J. Investig. Dermatol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"5760","DOI":"10.4049\/jimmunol.171.11.5760","article-title":"Human CD25+ regulatory T cells maintain immune tolerance to nickel in healthy, nonallergic individuals","volume":"17","author":"Cavani","year":"2003","journal-title":"J. Immunol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"4488","DOI":"10.4049\/jimmunol.177.7.4488","article-title":"The majority of human peripheral blood CD4 CD25h Foxp3 regulatory T cells bear functional skin-homing receptors","volume":"177","author":"Hirahara","year":"2006","journal-title":"J. Immunol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1038\/ni.2682","article-title":"Inflammation-induced interstitial migration of effector CD4+ T cells is dependent on integrin \u03b1V","volume":"14","author":"Overstreet","year":"2013","journal-title":"Nat. Immunol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1038\/nri.2015.1","article-title":"Regulatory T cell memory","volume":"16","author":"Rosenblum","year":"2016","journal-title":"Nat. Rev. Immunol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"255","DOI":"10.5603\/FHC.2011.0035","article-title":"CD4+CD25+ and CD4+CD2+high regulatory T cells in disseminated and localized forms of allergic contact dermatitis: Relation to specific cytokines","volume":"49","author":"Reduta","year":"2011","journal-title":"Folia. Histochem. Cytobiol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.atherosclerosis.2014.07.004","article-title":"Osteopontin- a multi-modal marker and mediator in atherosclerotic vascular disease","volume":"236","author":"Wolak","year":"2014","journal-title":"Atherosclerosis"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1111\/imm.12791","article-title":"Regulatory T cells in skin","volume":"152","author":"Ali","year":"2017","journal-title":"Immunology"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.1007\/s10072-017-3019-1","article-title":"Osteopontin and clusterin levels in type 2 diabetes mellitus: Differential association with peripheral autonomic nerve function","volume":"38","author":"Maser","year":"2017","journal-title":"Neurol. Sci."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1186\/s12931-016-0424-6","article-title":"Simvastatin up-regulates adenosine deaminase and suppresses osteopontin expression in COPD patients through an IL-13-dependent mechanism","volume":"17","author":"Maneechotesuwan","year":"2016","journal-title":"Respir. 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