{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T21:59:59Z","timestamp":1777067999114,"version":"3.51.4"},"reference-count":33,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,2,16]],"date-time":"2023-02-16T00:00:00Z","timestamp":1676505600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>This study aimed to assess clinical application of the Scheimpflug corneal tomography for objective evaluation of corneal optical density in eyes undergoing Descemet\u2019s stripping endothelial keratoplasty (DSEK). In this prospective study, 39 pseudophakic eyes with bullous keratopathy were enrolled. All eyes underwent primary DSEK. Ophthalmic examination included best corrected visual acuity (BCVA) measurement, biomicroscopy, Scheimpflug tomography, pachymetry, and endothelial cell count. All measurements were taken preoperatively and within a 2-year follow-up period. Gradual BCVA improvement was observed in all patients. After two years, the mean and median BCVA values were 0.18 logMAR. A decrease in central corneal thickness was noted only during the first 3 months postoperatively and was followed by a gradual increase. Corneal densitometry decreased constantly and most significantly in the first 3 months postoperatively. The consecutive decrease in endothelial cell count of the transplanted cornea was most significant during the first 6 months postoperatively. Six months postoperatively, the strongest correlation (Spearman\u2019s r = \u22120.41) with BCVA was found for densitometry. This tendency was maintained throughout the entire follow-up period. Corneal densitometry is applicable for objective monitoring of early and late outcomes of endothelial keratoplasty, showing a higher correlation with visual acuity than pachymetry and endothelial cell density.<\/jats:p>","DOI":"10.3390\/jcm12041552","type":"journal-article","created":{"date-parts":[[2023,2,16]],"date-time":"2023-02-16T01:36:52Z","timestamp":1676511412000},"page":"1552","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Corneal Optical Densitometry in the Evaluation of 2-Year Graft Function Following Endothelial Keratoplasty"],"prefix":"10.3390","volume":"12","author":[{"given":"Ilona","family":"Piotrowiak-S\u0142upska","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Division of Ophthalmology and Optometry, Nicolaus Copernicus University, 85-168 Bydgoszcz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1332-3592","authenticated-orcid":false,"given":"Bart\u0142omiej J.","family":"Ka\u0142u\u017cny","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Division of Ophthalmology and Optometry, Nicolaus Copernicus University, 85-168 Bydgoszcz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gra\u017cyna","family":"Malukiewicz","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Division of Ophthalmology and Optometry, Nicolaus Copernicus University, 85-168 Bydgoszcz, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1113\/jphysiol.1957.sp005758","article-title":"The structure and transparency of the cornea","volume":"136","author":"Maurice","year":"1957","journal-title":"J. Physiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1113\/jphysiol.1970.sp009230","article-title":"The transparency of the mammalian cornea","volume":"210","author":"Cox","year":"1970","journal-title":"J. Physiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1113\/jphysiol.1973.sp010325","article-title":"Wave-length dependencies of light scattering in normal and cold swollen rabbit corneas and their structural implications","volume":"233","author":"Farrell","year":"1973","journal-title":"J. Physiol."},{"key":"ref_4","unstructured":"Kaufman, P.L., and Alm, A. (2003). Adler\u2019s Physiology of the Eye, Mosby. [10th ed.]."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1527","DOI":"10.1016\/j.ophtha.2021.04.015","article-title":"Hyperosmolar eye Drops for Diurnal Corneal Edema in Fuchs\u2019 Endothelial Dystrophy","volume":"128","author":"Zander","year":"2021","journal-title":"Ophthalmology"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.ajo.2021.03.006","article-title":"Randomized, Double-Masked, Pilot Study of Netarsudil 0.02% Ophthalmic Solution for Treatment of Corneal Edema in Fuchs Dystrophy","volume":"227","author":"Price","year":"2021","journal-title":"Am. J. Ophthalmol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1097\/01.ico.0000244869.54761.50","article-title":"Endothelial keratoplasty: History, current state, and future directions","volume":"25","author":"Terry","year":"2006","journal-title":"Cornea"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1007\/s00347-020-01134-1","article-title":"Descemet membrane endothelial keratoplasty DMEK\u2014Spender und Empf\u00e4nger Schritt f\u00fcr Schritt","volume":"117","author":"Seitz","year":"2020","journal-title":"Ophthalmologe"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1097\/00003226-200104000-00001","article-title":"Deep lamellar endothelial keratoplasty in the first United States patients: Early clinical results","volume":"20","author":"Terry","year":"2001","journal-title":"Cornea"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1097\/00003226-200204000-00018","article-title":"Sutureless, posterior lamellar keratoplasty: A case report of a modified technique","volume":"21","author":"Melles","year":"2002","journal-title":"Cornea"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"339","DOI":"10.3928\/1081-597X-20050701-07","article-title":"Descemet\u2019s stripping with endothelial keratoplasty in 50 eyes: A refractive neutral corneal transplant","volume":"21","author":"Price","year":"2005","journal-title":"J. Refract. Surg."},{"key":"ref_12","first-page":"987","article-title":"Descemet membrane endothelial keratoplasty (DMEK)","volume":"25","author":"Melles","year":"2006","journal-title":"Cornea"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1186","DOI":"10.1016\/j.ophtha.2012.11.030","article-title":"Ultrathin descemet\u2019s stripping automated endothelial keratoplasty with the microkeratome double-pass technique: Two-year outcomes","volume":"120","author":"Busin","year":"2013","journal-title":"Ophthalmology"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1167\/iovs.13-13236","article-title":"Normative values for corneal densitometry analysis by Scheimpflug optical assessment","volume":"55","author":"Rozema","year":"2014","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1007\/s10792-013-9736-4","article-title":"Corneal densitometry evaluation in archipelago keratitis","volume":"34","author":"Talaz","year":"2014","journal-title":"Int. Ophthalmol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"781","DOI":"10.3928\/1081597X-20121011-06","article-title":"Corneal transparency after cross-linking for keratoconus: 1-year follow-up","volume":"28","author":"Lopez","year":"2012","journal-title":"J. Refract. Surg."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2131","DOI":"10.1016\/j.jcrs.2012.07.041","article-title":"Wavefront-optimized excimer laser in situ keratomileusis for myopia and myopic astigmatism: Refractive outcomes and corneal densitometry","volume":"38","author":"Fares","year":"2012","journal-title":"J. Cataract. Refract. Surg."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1136\/bjophthalmol-2011-300214","article-title":"Outcomes of deep anterior lamellar keratoplasty following successful and failed \u2018big bubble\u2019","volume":"96","author":"Bhatt","year":"2012","journal-title":"Br. J. Ophthalmol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4797","DOI":"10.1167\/iovs.12-10003","article-title":"Visual performance with wave aberration correction after penetrating, deep anterior lamellar, or endothelial keratoplasty","volume":"53","author":"Pantanelli","year":"2012","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1278","DOI":"10.1097\/ICO.0b013e3181cda01a","article-title":"Secondary DMEK for poor visual outcome after DSEK: Donor posterior stroma may limit visual acuity in endothelial keratoplasty","volume":"29","author":"Ham","year":"2010","journal-title":"Cornea"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1111\/j.1755-3768.2012.02504.x","article-title":"Identifying causes for poor visual outcome after DSEK\/DSAEK following secondary DMEK in the same eye","volume":"91","author":"Dirisamer","year":"2013","journal-title":"Acta Ophthalmol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1016\/j.ajo.2011.05.003","article-title":"Graft thickness, graft folds, and aberrations after Descemet stripping endothelial keratoplasty for Fuchs dystrophy","volume":"152","author":"Seery","year":"2011","journal-title":"Am. J. Ophthalmol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1016\/j.ophtha.2011.08.034","article-title":"Corneal higher-order aberrations after descemet\u2019s membrane endothelial keratoplasty","volume":"119","author":"Rudolph","year":"2012","journal-title":"Ophthalmology"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"6222","DOI":"10.1167\/iovs.11-7647","article-title":"The contribution of the posterior surface to the corneal aberrations in eyes after keratoplasty","volume":"52","author":"Yamaguchi","year":"2011","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.ajo.2014.04.008","article-title":"Optical quality of the cornea after descemet membrane endothelial keratoplasty","volume":"158","author":"Droutsas","year":"2014","journal-title":"Am. J. Ophthalmol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1097\/ICO.0000000000000078","article-title":"Descemet membrane endothelial keratoplasty combined with epithelial debridement and mitomycin-C application for Fuchs dystrophy with preoperative subepithelial fibrosis or anterior basement membrane dystrophy","volume":"33","author":"Chaurasia","year":"2014","journal-title":"Cornea"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1097\/ICO.0000000000001483","article-title":"Scheimpflug densitometry after DMEK versus DSAEK-two-year outcomes","volume":"37","author":"Droutsas","year":"2018","journal-title":"Cornea"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2575","DOI":"10.1007\/s00417-013-2460-y","article-title":"Comparison of corneal haze and visual outcome in primary DSAEK versus DSAEK following failed DMEK","volume":"251","author":"Oblanca","year":"2013","journal-title":"Graefes Arch. Clin. Exp. Ophthalmol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1136\/bjophthalmol-2013-304042","article-title":"Recipient corneal thickness and visual outcome after descemet\u2019s stripping automated endothelial keratoplasty","volume":"98","author":"Ivarsen","year":"2014","journal-title":"Br. J. Ophthalmol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1818","DOI":"10.1016\/j.ophtha.2009.06.021","article-title":"Descemet\u2019s stripping endothelial keratoplasty: Safety and outcomes: A report by the American Academy of Ophthalmology","volume":"116","author":"Lee","year":"2009","journal-title":"Ophthalmology"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/j.exer.2010.06.021","article-title":"The molecular basis of corneal transparency","volume":"91","author":"Hassell","year":"2010","journal-title":"Exp. Eye Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1016\/S0014-4835(69)80042-4","article-title":"Studies on corneal polysaccharides. V. Changes in corneal glycosaminoglycans in transient stromal edema","volume":"8","author":"Anseth","year":"1969","journal-title":"Exp. Eye Res."},{"key":"ref_33","first-page":"1994","article-title":"Loss of stromal glycosaminoglycans during corneal edema","volume":"31","author":"Kangas","year":"1990","journal-title":"Investig. Ophthalmol. Vis. Sci."}],"container-title":["Journal of Clinical Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2077-0383\/12\/4\/1552\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:37:20Z","timestamp":1760121440000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2077-0383\/12\/4\/1552"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,16]]},"references-count":33,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["jcm12041552"],"URL":"https:\/\/doi.org\/10.3390\/jcm12041552","relation":{},"ISSN":["2077-0383"],"issn-type":[{"value":"2077-0383","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,16]]}}}