{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T04:12:26Z","timestamp":1776399146044,"version":"3.51.2"},"reference-count":32,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2023,4,27]],"date-time":"2023-04-27T00:00:00Z","timestamp":1682553600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Grant-in-Aid for Scientific Research (KAKENHI) from Japan Society for the Promotion of Science (JSPS)","award":["19K18018"],"award-info":[{"award-number":["19K18018"]}]},{"name":"Grant-in-Aid for Scientific Research (KAKENHI) from Japan Society for the Promotion of Science (JSPS)","award":["20K17546"],"award-info":[{"award-number":["20K17546"]}]},{"name":"Grant-in-Aid for Scientific Research (KAKENHI) from Japan Society for the Promotion of Science (JSPS)","award":["22K20933"],"award-info":[{"award-number":["22K20933"]}]},{"name":"Grant-in-Aid for Scientific Research (KAKENHI) from Japan Society for the Promotion of Science (JSPS)","award":["20H03737"],"award-info":[{"award-number":["20H03737"]}]},{"name":"Ministry of Education, Culture and Technology (MEXT) of Japan","award":["19K18018"],"award-info":[{"award-number":["19K18018"]}]},{"name":"Ministry of Education, Culture and Technology (MEXT) of Japan","award":["20K17546"],"award-info":[{"award-number":["20K17546"]}]},{"name":"Ministry of Education, Culture and Technology (MEXT) of Japan","award":["22K20933"],"award-info":[{"award-number":["22K20933"]}]},{"name":"Ministry of Education, Culture and Technology (MEXT) of Japan","award":["20H03737"],"award-info":[{"award-number":["20H03737"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Warm ischemia-reperfusion injury is a prognostic factor for hepatectomy and liver transplantation. However, its underlying molecular mechanisms are unknown. This study aimed to elucidate these mechanisms and identify the predictive markers of post-reperfusion injury. Rats with normal livers were subjected to 70% hepatic warm ischemia for 15, 30, or 90 min, while those with steatotic livers were subjected to 70% hepatic warm ischemia for only 30 min. The liver and blood were sampled at the end of ischemia and 1, 6, and 24 h after reperfusion. The serum alanine aminotransferase (ALT) activity, Suzuki injury scores, and lipid peroxidation (LPO) products were evaluated. The ALT activity and Suzuki scores increased with ischemic duration and peaked at 1 and 6 h after reperfusion, respectively. Steatotic livers subjected to 30 min ischemia and normal livers subjected to 90 min ischemia showed comparable injury. A similar trend was observed for LPO products. Imaging mass spectrometry of normal livers revealed an increase in lysophosphatidylinositol (LPI (18:0)) and a concomitant decrease in phosphatidylinositol (PI (18:0\/20:4)) in Zone 1 (central venous region) with increasing ischemic duration; they returned to their basal values after reperfusion. Similar changes were observed in steatotic livers. Hepatic warm ischemia time-dependent acceleration of PI (18:0\/20:4) to LPI (18:0) conversion occurs initially in Zone 1 and is more pronounced in fatty livers. Thus, the LPI (18:0)\/PI (18:0\/20:4) ratio is a potential predictor of post-reperfusion injury.<\/jats:p>","DOI":"10.3390\/jcm12093163","type":"journal-article","created":{"date-parts":[[2023,4,28]],"date-time":"2023-04-28T02:02:23Z","timestamp":1682647343000},"page":"3163","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Warm Ischemia Induces Spatiotemporal Changes in Lysophosphatidylinositol That Affect Post-Reperfusion Injury in Normal and Steatotic Rat Livers"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7776-2068","authenticated-orcid":false,"given":"Kengo","family":"Shibata","sequence":"first","affiliation":[{"name":"Department of Gastroenterological Surgery I, Graduate School of Medicine, Hokkaido University, Sapporo 060-8638, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takahiro","family":"Hayasaka","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery I, Graduate School of Medicine, Hokkaido University, Sapporo 060-8638, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5942-1211","authenticated-orcid":false,"given":"Sodai","family":"Sakamoto","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery I, Graduate School of Medicine, Hokkaido University, Sapporo 060-8638, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satsuki","family":"Hashimoto","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery I, Graduate School of Medicine, Hokkaido University, Sapporo 060-8638, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norio","family":"Kawamura","sequence":"additional","affiliation":[{"name":"Department of Transplant Surgery, Graduate School of Medicine, Hokkaido University, Sapporo 060-8638, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masato","family":"Fujiyoshi","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery I, Graduate School of Medicine, Hokkaido University, Sapporo 060-8638, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taichi","family":"Kimura","sequence":"additional","affiliation":[{"name":"Department of Cancer Pathology, Faculty of Medicine, Hokkaido University, Sapporo 060-8638, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1951-4015","authenticated-orcid":false,"given":"Tsuyoshi","family":"Shimamura","sequence":"additional","affiliation":[{"name":"Division of Organ Transplantation, Hokkaido University Hospital, Sapporo 060-8648, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8593-2258","authenticated-orcid":false,"given":"Moto","family":"Fukai","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery I, Graduate School of Medicine, Hokkaido University, Sapporo 060-8638, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akinobu","family":"Taketomi","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery I, Graduate School of Medicine, Hokkaido University, Sapporo 060-8638, Japan"},{"name":"Department of Transplant Surgery, Graduate School of Medicine, Hokkaido University, Sapporo 060-8638, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1053\/jhep.2000.9323","article-title":"Inflammatory mechanisms and therapeutic strategies for warm hepatic ischemia\/reperfusion injury","volume":"32","author":"Lentsch","year":"2000","journal-title":"Hepatology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1002\/lt.23547","article-title":"Declining liver utilization for transplantation in the United States and the impact of donation after cardiac death","volume":"19","author":"Orman","year":"2013","journal-title":"Liver Transpl."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1097\/00007890-199501000-00008","article-title":"Experience with liver and kidney allografts from non-heart-beating donors","volume":"59","author":"Casavilla","year":"1995","journal-title":"Transplantation"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1111\/tri.12776","article-title":"New classification of donation after circulatory death donors definitions and terminology","volume":"29","author":"Thuong","year":"2016","journal-title":"Transpl. 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