{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T15:33:37Z","timestamp":1776440017530,"version":"3.51.2"},"reference-count":23,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,5,20]],"date-time":"2023-05-20T00:00:00Z","timestamp":1684540800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FNRS Belgium","award":["T.0008.15"],"award-info":[{"award-number":["T.0008.15"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Transplantation of heart following donation after circulatory death (DCD) was recently introduced into clinical practice. Ex vivo reperfusion following DCD and retrieval is deemed necessary in order to evaluate the recovery of cardiac viability after the period of warm ischemia. We tested the effect of four different temperatures (4 \u00b0C\u201418 \u00b0C\u201425 \u00b0C\u201435 \u00b0C) on cardiac metabolism during 3-h ex vivo reperfusion in a porcine model of DCD heart. We observed a steep fall in high-energy phosphate (ATP) concentrations in the myocardial tissue at the end of the warm ischemic time and only limited regeneration during reperfusion. Lactate concentration in the perfusate increased rapidly during the first hour of reperfusion and slowly decreased afterward. However, the temperature of the solution does not seem to have an effect on either ATP or lactate concentration. Furthermore, all cardiac allografts showed a significant weight increase due to cardiac edema, regardless of the temperature.<\/jats:p>","DOI":"10.3390\/jcm12103569","type":"journal-article","created":{"date-parts":[[2023,5,22]],"date-time":"2023-05-22T02:00:27Z","timestamp":1684720827000},"page":"3569","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Comparison of Different Ex-Vivo Preservation Strategies on Cardiac Metabolism in an Animal Model of Donation after Circulatory Death"],"prefix":"10.3390","volume":"12","author":[{"given":"Stefano","family":"Mastrobuoni","sequence":"first","affiliation":[{"name":"Cardiovascular and Thoracic Surgery Department, Saint-Luc\u2019s Hospital, Catholic University of Louvain, 1200 Brussels, Belgium"},{"name":"Pole de Chirurgie Exp\u00e9rimentale et Transplantation, Institut de Recherche Exp\u00e9rimentale et Clinique (IREC), Catholic University of Louvain, 1200 Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2536-5949","authenticated-orcid":false,"given":"Manuel","family":"Johanns","sequence":"additional","affiliation":[{"name":"Pole de Biochimie et Recherche Metabolique, Institue de Duve, Catholic University of Louvain, 1200 Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martial","family":"Vergauwen","sequence":"additional","affiliation":[{"name":"Pole de Chirurgie Exp\u00e9rimentale et Transplantation, Institut de Recherche Exp\u00e9rimentale et Clinique (IREC), Catholic University of Louvain, 1200 Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gwen","family":"Beaurin","sequence":"additional","affiliation":[{"name":"Pole de Chirurgie Exp\u00e9rimentale et Transplantation, Institut de Recherche Exp\u00e9rimentale et Clinique (IREC), Catholic University of Louvain, 1200 Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Rider","sequence":"additional","affiliation":[{"name":"Pole de Biochimie et Recherche Metabolique, Institue de Duve, Catholic University of Louvain, 1200 Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre","family":"Gianello","sequence":"additional","affiliation":[{"name":"Pole de Chirurgie Exp\u00e9rimentale et Transplantation, Institut de Recherche Exp\u00e9rimentale et Clinique (IREC), Catholic University of Louvain, 1200 Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3220-2681","authenticated-orcid":false,"given":"Alain","family":"Poncelet","sequence":"additional","affiliation":[{"name":"Cardiovascular and Thoracic Surgery Department, Saint-Luc\u2019s Hospital, Catholic University of Louvain, 1200 Brussels, Belgium"},{"name":"Pole de Chirurgie Exp\u00e9rimentale et Transplantation, Institut de Recherche Exp\u00e9rimentale et Clinique (IREC), Catholic University of Louvain, 1200 Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivier","family":"Van Caenegem","sequence":"additional","affiliation":[{"name":"Pole de Chirurgie Exp\u00e9rimentale et Transplantation, Institut de Recherche Exp\u00e9rimentale et Clinique (IREC), Catholic University of Louvain, 1200 Brussels, Belgium"},{"name":"Cardiac Intensive Care Unit, Saint-Luc\u2019s Hospital, Catholic University of Louvain, 1200 Brussels, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.1016\/j.healun.2020.10.001","article-title":"A 5-year single-center early experience of heart transplantation from donation after circulatory-determined death donors","volume":"39","author":"Messer","year":"2020","journal-title":"J. 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