{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:14:11Z","timestamp":1760148851104,"version":"build-2065373602"},"reference-count":18,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,6,3]],"date-time":"2023-06-03T00:00:00Z","timestamp":1685750400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>The study of the placenta is of great importance, not only in the attempt to understand the etiopathogenesis of various maternal-fetal pathologies, but also in the attempt to understand whether it is possible to find the cause of pathological neonatal outcomes. On the other hand, abnormalities of blood vessel formation, such as angiodysplasias, have been poorly characterised in the literature, and there is a need for more studies investigating the potential impact on the fetus. In this paper, we retrospectively analysed 2063 placentas received at the Department of Pathology of the University of Bari \u2018Aldo Moro\u2019, among which we identified 70 placentas affected by angiodysplasia. On these placentas, we carried out histochemical staining with Masson\u2019s Trichrome, orcein-alcian blue, and, subsequently, immunostaining with anti-CD31, CD34, and desmin and actin muscle smoothness antibodies. Finally, we performed a morphometric analysis on the allantochorionic and truncal vessels and correlated the results with neonatal outcomes. We studied the characteristics of the angiodysplasias in detail, dividing the patients into two classes (A and B) according to the morphology and histochemical characteristics of the affected vessels; statistical analysis reported a statistically significant association (p &lt; 0.05) between the ratio of maximum thickness to maximum diameter (Tmax\/Dmax) and neonatal outcome, with only 30% physiological outcome in the cohort of the placentas affected by angiodysplasia. These results shed light on a rather neglected aspect in the 2015 Amsterdam Classification, as well as in the literature, and provided strong evidence that placental angiodysplasia is predictive of an increased likelihood of the pathological fetal outcome, while other factors remain in the field. Studies with larger case series and guidelines with more attention to these aspects are mandated to further investigate the predictive potential of this pathology.<\/jats:p>","DOI":"10.3390\/jcm12113835","type":"journal-article","created":{"date-parts":[[2023,6,5]],"date-time":"2023-06-05T02:18:29Z","timestamp":1685931509000},"page":"3835","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Placental Angiodysplasia: A New Sign for Prediction of Fetal Outcome?"],"prefix":"10.3390","volume":"12","author":[{"given":"Andrea","family":"Marzullo","sequence":"first","affiliation":[{"name":"Section of Molecular Pathology, Department of Precision and Regenerative Medicine and Ionian Area (DiMePRe-J), University of Bari \u201cAldo Moro\u201d, 70124 Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emmanuela","family":"Vitelli","sequence":"additional","affiliation":[{"name":"Section of Molecular Pathology, Department of Precision and Regenerative Medicine and Ionian Area (DiMePRe-J), University of Bari \u201cAldo Moro\u201d, 70124 Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0325-4316","authenticated-orcid":false,"given":"Gerardo","family":"Cazzato","sequence":"additional","affiliation":[{"name":"Section of Molecular Pathology, Department of Precision and Regenerative Medicine and Ionian Area (DiMePRe-J), University of Bari \u201cAldo Moro\u201d, 70124 Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0128-9745","authenticated-orcid":false,"given":"Margherita","family":"Fanelli","sequence":"additional","affiliation":[{"name":"Section of Molecular Pathology, Department of Precision and Regenerative Medicine and Ionian Area (DiMePRe-J), University of Bari \u201cAldo Moro\u201d, 70124 Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4792-3545","authenticated-orcid":false,"given":"Giuseppe","family":"Ingravallo","sequence":"additional","affiliation":[{"name":"Section of Molecular Pathology, Department of Precision and Regenerative Medicine and Ionian Area (DiMePRe-J), University of Bari \u201cAldo Moro\u201d, 70124 Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9862-1619","authenticated-orcid":false,"given":"Antonella","family":"Vimercati","sequence":"additional","affiliation":[{"name":"Section of Gynaecology and Obstetrics, Department of Precision and Regenerative Medicine and Ionian Area (DiMePRe-J), University of Bari \u201cAldo Moro\u201d, 70124 Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberta","family":"Rossi","sequence":"additional","affiliation":[{"name":"Section of Molecular Pathology, Department of Precision and Regenerative Medicine and Ionian Area (DiMePRe-J), University of Bari \u201cAldo Moro\u201d, 70124 Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4335-4776","authenticated-orcid":false,"given":"Leonardo","family":"Resta","sequence":"additional","affiliation":[{"name":"Section of Molecular Pathology, Department of Precision and Regenerative Medicine and Ionian Area (DiMePRe-J), University of Bari \u201cAldo Moro\u201d, 70124 Bari, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Asher, R.J. (2007). A web-database of mammalian morphology and a reanalysis of placental phylogeny. BMC Evol. Biol., 7.","DOI":"10.1186\/1471-2148-7-108"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1296","DOI":"10.1136\/jcp.2008.055277","article-title":"The anatomy of the normal placenta","volume":"61","author":"Huppertz","year":"2008","journal-title":"J. Clin. Pathol."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Malvasi, A., Tinelli, A., and Di Renzo, G.C. (2017). Management and Therapy of Late Pregnancy Complications, Springer International Publishing AG.","DOI":"10.1007\/978-3-319-48732-8"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1007\/BF01574466","article-title":"Hemorrhage from the large bowel with special reference to angiodysplasia and diverticular disease","volume":"2","author":"Welch","year":"1978","journal-title":"World J. Surg."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1001\/archinte.1995.00430080037005","article-title":"Angiodysplasia and lower gastrointestinal tract bleeding in elderly patients","volume":"155","author":"Sharma","year":"1995","journal-title":"Arch. Intern. Med."},{"key":"ref_6","first-page":"59","article-title":"Micromorphological and clinical aspects of angiodysplasias","volume":"33","author":"Kluken","year":"1980","journal-title":"Phlebologie"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e203","DOI":"10.1542\/peds.2014-3673","article-title":"Vascular Anomalies Classification: Recommendations from the International Society for the Study of Vascular Anomalies","volume":"136","author":"Wassef","year":"2015","journal-title":"Pediatrics"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1016\/j.placenta.2010.09.005","article-title":"Placental surface shape, function, and effects of maternal and fetal vascular pathology","volume":"31","author":"Salafia","year":"2010","journal-title":"Placenta"},{"key":"ref_9","first-page":"e2020061","article-title":"Analysis of relation between placental lesions and perinatal outcome according to Amsterdam criteria: A comparative study","volume":"91","author":"Loverro","year":"2020","journal-title":"Acta BioMed"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1016\/j.redox.2014.05.001","article-title":"Genetically induced oxidative stress in mice causes thrombocytosis, splenomegaly and placental angiodysplasia that leads to recurrent abortion","volume":"2","author":"Ishii","year":"2014","journal-title":"Redox Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1007\/s10024-003-8083-2","article-title":"Society for Pediatric Pathology, Perinatal Section, Maternal Vascular Perfusion Nosology Committee. Maternal Vascular Underperfusion: Nosology and Reproducibility of Placental Reaction Patterns","volume":"7","author":"Redline","year":"2004","journal-title":"Pediatr. Dev. Pathol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1007\/s10024-004-2020-x","article-title":"Fetal Vascular Obstructive Lesions: Nosology and Reproducibility of Placental Reaction Patterns","volume":"7","author":"Redline","year":"2004","journal-title":"Pediatr. Dev. Pathol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.1016\/j.humpath.2007.05.025","article-title":"Villitis of unknown etiology: Noninfectious chronic villitis in the placenta","volume":"38","author":"Redline","year":"2007","journal-title":"Hum. Pathol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.placenta.2020.05.003","article-title":"The placenta in fetal growth restriction: What is going wrong?","volume":"96","author":"Sun","year":"2020","journal-title":"Placenta"},{"key":"ref_15","first-page":"OL79","article-title":"Characterization of first trimester human fetal placental vessels using immunocytochemical markers","volume":"47","author":"Challier","year":"2001","journal-title":"Cell Mol. Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1369\/jhc.4A6514.2005","article-title":"Immunohistochemical Expression of Endothelial Markers CD31, CD34, von Willebrand Factor, and Fli-1 in Normal Human Tissues","volume":"54","author":"Pusztaszeri","year":"2006","journal-title":"J. Histochem. Cytochem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1620\/tjem.145.373","article-title":"Distribution of fibronectin and other connective tissue components in human placenta","volume":"145","author":"Isemura","year":"1985","journal-title":"Tohoku J. Exp. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1007\/BF01003632","article-title":"The Alcian Blue and combined Alcian Blue-Safranin O staining of glycosaminoglycans studied in a model system and in mast cells","volume":"9","author":"Tas","year":"1977","journal-title":"Histochem. J."}],"container-title":["Journal of Clinical Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2077-0383\/12\/11\/3835\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:48:27Z","timestamp":1760125707000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2077-0383\/12\/11\/3835"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,3]]},"references-count":18,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2023,6]]}},"alternative-id":["jcm12113835"],"URL":"https:\/\/doi.org\/10.3390\/jcm12113835","relation":{},"ISSN":["2077-0383"],"issn-type":[{"type":"electronic","value":"2077-0383"}],"subject":[],"published":{"date-parts":[[2023,6,3]]}}}