{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T14:05:21Z","timestamp":1770818721924,"version":"3.50.1"},"reference-count":39,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T00:00:00Z","timestamp":1700697600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Preterm premature rupture of membranes (pPROM) stands as a primary contributor to preterm deliveries worldwide, closely linked to consequential infectious peripartum complications, including chorioamnionitis and early-onset neonatal sepsis. As a prophylactic measure, individuals following pPROM routinely undergo antibiotic treatment. The aim of this study was to evaluate changes in the vaginal microbial colonization after antibiotic treatment following pPROM. Therefore, we retrospectively assessed the impact of antibiotic treatment on the maternal vaginal microbial colonization in 438 post-pPROM patients delivering before 29 gestational weeks. Vaginal samples were collected for microbiological analysis before and after antibiotic administration and analysed for seventeen pre-defined microbial groups. We observed eradication in eleven microbial groups, including beta-hemolytic streptococci group B and Gardnerella vaginalis. No significant reduction was found for the remaining groups, including Escherichia (E.) coli. Moreover, we found a notable increase in resistant bacteria after antibiotic treatment. In conclusion, broad-spectrum antimicrobial treatment exhibited substantial efficacy in eradicating the majority of pathogens in our cohort. However, certain pathogens, notably E. coli, showed resilience. Given E. coli\u2019s prominent role in infectious peripartum complications, our findings underline the challenges in antibiotic management post-pPROM and the need to establish international guidelines, particularly regarding emerging concerns about antibiotic resistances.<\/jats:p>","DOI":"10.3390\/jcm12237249","type":"journal-article","created":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T03:45:56Z","timestamp":1700711156000},"page":"7249","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Vaginal Microbial Colonization after Antibiotic Treatment in Women with Preterm Premature Rupture of Membranes: An Observational Cohort Study"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5062-0097","authenticated-orcid":false,"given":"Fanny","family":"Mikula","sequence":"first","affiliation":[{"name":"Division of Obstetrics and Feto-Maternal Medicine, Department of Obstetrics and Gynecology, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melanie","family":"Wimmer","sequence":"additional","affiliation":[{"name":"Division of Obstetrics and Feto-Maternal Medicine, Department of Obstetrics and Gynecology, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4628-9052","authenticated-orcid":false,"given":"Alex","family":"Farr","sequence":"additional","affiliation":[{"name":"Division of Obstetrics and Feto-Maternal Medicine, Department of Obstetrics and Gynecology, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harald","family":"Leitich","sequence":"additional","affiliation":[{"name":"Division of Obstetrics and Feto-Maternal Medicine, Department of Obstetrics and Gynecology, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Ebner","sequence":"additional","affiliation":[{"name":"Department of Infection Control and Hospital Epidemiology, Medical University Vienna, 1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnes","family":"Grill","sequence":"additional","affiliation":[{"name":"Division of Neonatology, Pediatric Intensive Care and Neuropediatrics, Department of Pediatrics and Adolescent Medicine, Comprehensive Center for Pediatrics, Medical University Vienna, 1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonja","family":"Granser","sequence":"additional","affiliation":[{"name":"Division of Obstetrics and Feto-Maternal Medicine, Department of Obstetrics and Gynecology, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8409-1119","authenticated-orcid":false,"given":"Philipp","family":"Foessleitner","sequence":"additional","affiliation":[{"name":"Division of Obstetrics and Feto-Maternal Medicine, Department of Obstetrics and Gynecology, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,23]]},"reference":[{"key":"ref_1","unstructured":"WHO (2023, November 20). Preterm Birth, Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/preterm-birth."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/S0140-6736(08)60074-4","article-title":"Epidemiology and causes of preterm birth","volume":"371","author":"Goldenberg","year":"2008","journal-title":"Lancet"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/j.puhe.2014.03.010","article-title":"Outcomes in preterm infants","volume":"128","author":"Platt","year":"2014","journal-title":"Public Health"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/S0140-6736(08)60136-1","article-title":"An overview of mortality and sequelae of preterm birth from infancy to adulthood","volume":"371","author":"Saigal","year":"2008","journal-title":"Lancet"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"ACOG (2018). Committee on Practice Bulletins-Obstetrics. Practice Bulletin No. 188: Prelabor Rupture of Membranes. Obstet. Gynecol., 131, e1\u2013e14.","DOI":"10.1097\/AOG.0000000000002663"},{"key":"ref_6","first-page":"304","article-title":"Prevention and Therapy of Preterm Birth. Guideline of the DGGG, OEGGG and SGGG (S2k Level, AWMF Registry Number 015\/025, February 2019)\u2014Part 1 with Recommendations on the Epidemiology, Etiology, Prediction, Primary and Secondary Prevention of Preterm Birth","volume":"223","author":"Berger","year":"2019","journal-title":"Z. Geburtshilfe Neonatol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1515\/jpm-2017-0027","article-title":"Mid-trimester preterm premature rupture of membranes (PPROM): Etiology, diagnosis, classification, international recommendations of treatment options and outcome","volume":"46","author":"Tchirikov","year":"2018","journal-title":"J. Perinat. Med."},{"key":"ref_8","unstructured":"AWMF (2023, November 20). Pr\u00e4vention und Therapie der Fr\u00fchgeburt. 015\u2013025 2022. Available online: https:\/\/register.awmf.org\/assets\/guidelines\/015-025l_S2k_Praevention-Therapie-Fruehgeburt_2022-09.pdf."},{"key":"ref_9","unstructured":"National Institute for Health and Clinical Excellence (2022). Preterm Labour and Birth, National Institute for Health and Care Excellence (NICE)."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Kenyon, S., Boulvain, M., and Neilson, J.P. (2013). Antibiotics for preterm rupture of membranes. Cochrane Database Syst. Rev., Cd001058.","DOI":"10.1002\/14651858.CD001058.pub3"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Kacerovsky, M., Vrbacky, F., Kutova, R., Pliskova, L., Andrys, C., Musilova, I., Menon, R., Lamont, R., and Nekvindova, J. (2015). Cervical microbiota in women with preterm prelabor rupture of membranes. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0126884"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Paramel Jayaprakash, T., Wagner, E.C., van Schalkwyk, J., Albert, A.Y., Hill, J.E., and Money, D.M. (2016). High Diversity and Variability in the Vaginal Microbiome in Women following Preterm Premature Rupture of Membranes (PPROM): A Prospective Cohort Study. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0166794"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1055\/s-0032-1328132","article-title":"Prevalence, Spectrum and Antibiotic Susceptibility of Bacterial and Candida Colonization between the 21st and 33rd Week of Gestation in Women with PPROM\u20145 Years\u2019 Experience in 1 Perinatal Center","volume":"73","author":"Reinhard","year":"2013","journal-title":"Geburtshilfe Frauenheilkd."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"341","DOI":"10.29252\/ijrm.16.5.341","article-title":"Cervical bacterial colonization in women with preterm premature rupture of membrane and pregnancy outcomes: A cohort study","volume":"16","author":"Saghafi","year":"2018","journal-title":"Int. J. Reprod. Biomed."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Hofer, U. (2019). The cost of antimicrobial resistance. Nat. Rev. Microbiol., 17.","DOI":"10.1038\/s41579-019-0161-1"},{"key":"ref_16","first-page":"287","article-title":"Pathogens in preterm prelabour rupture of membranes and erythromycin for antibiotic prophylaxis: A retrospective analysis","volume":"25","author":"Li","year":"2019","journal-title":"Hong Kong Med. J."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1016\/S0140-6736(00)04233-1","article-title":"Broad-spectrum antibiotics for preterm, prelabour rupture of fetal membranes: The ORACLE I randomised trial. ORACLE Collaborative Group","volume":"357","author":"Kenyon","year":"2001","journal-title":"Lancet"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1055\/a-2169-8539","article-title":"Bacterial Vaginosis: Guideline of the DGGG, OEGGG and SGGG (S2k-Level, AWMF Registry No. 015\/028, June 2023)","volume":"83","author":"Farr","year":"2023","journal-title":"Geburtshilfe Und Frauenheilkd."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1111\/1469-0691.12411","article-title":"Antibiotic use during pregnancy alters the commensal vaginal microbiota","volume":"20","author":"Stokholm","year":"2014","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1528","DOI":"10.1128\/AAC.50.4.1528-1530.2006","article-title":"Microflora changes with norfloxacin and pivmecillinam in women with recurrent urinary tract infection","volume":"50","author":"Norinder","year":"2006","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1016\/j.ogc.2020.08.001","article-title":"Vaginal Microbiome in Preterm Rupture of Membranes","volume":"47","author":"Bennett","year":"2020","journal-title":"Obstet. Gynecol. Clin. N. Am."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e1398","DOI":"10.7717\/peerj.1398","article-title":"Persistent microbial dysbiosis in preterm premature rupture of membranes from onset until delivery","volume":"3","author":"Baldwin","year":"2015","journal-title":"PeerJ"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1038\/s41579-023-00936-9","article-title":"Antibiotic-induced collateral damage to the microbiota and associated infections","volume":"21","author":"Kobras","year":"2023","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_24","first-page":"419","article-title":"The risks of symptomatic vaginal candidiasis after oral antibiotic therapy","volume":"86","author":"MacDonald","year":"1993","journal-title":"Q. J. Med."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"261","DOI":"10.3122\/jabfm.2008.04.070169","article-title":"Effect of antibiotics on vulvovaginal candidiasis: A MetroNet study","volume":"21","author":"Xu","year":"2008","journal-title":"J. Am. Board Fam. Med."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"S29","DOI":"10.1016\/j.ajog.2015.08.040","article-title":"Acute chorioamnionitis and funisitis: Definition, pathologic features, and clinical significance","volume":"213","author":"Kim","year":"2015","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1038\/s41390-021-01633-0","article-title":"Chorioamnionitis and neonatal outcomes","volume":"91","author":"Jain","year":"2022","journal-title":"Pediatr. Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e20182896","DOI":"10.1542\/peds.2018-2896","article-title":"Management of Neonates Born at \u226434 6\/7 Weeks\u2019 Gestation With Suspected or Proven Early-Onset Bacterial Sepsis","volume":"142","author":"Puopolo","year":"2018","journal-title":"Pediatrics"},{"key":"ref_29","first-page":"Cd013837","article-title":"Antibiotic regimens for early-onset neonatal sepsis","volume":"5","author":"Korang","year":"2021","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.tjog.2012.01.006","article-title":"Characteristics of early-onset neonatal sepsis caused by Escherichia coli","volume":"51","author":"Tsai","year":"2012","journal-title":"Taiwan J. Obstet. Gynecol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1111\/ajo.13087","article-title":"Patterns of placental antimicrobial resistance in preterm birth before 30 completed weeks gestation complicated by preterm prelabour rupture of membranes","volume":"60","author":"Axford","year":"2020","journal-title":"Aust. N. Z. J. Obstet. Gynaecol."},{"key":"ref_32","first-page":"49","article-title":"Effect of intrapartum antibiotic prophylaxis of group B streptococcus infection on the incidence and bacteriological profile of early-onset neonatal sepsis","volume":"24","author":"Peng","year":"2022","journal-title":"Zhongguo Dang Dai Er Ke Za Zhi"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"854","DOI":"10.1067\/mob.2001.117354","article-title":"No increase in rates of early-onset neonatal sepsis by non-group B Streptococcus or ampicillin-resistant organisms","volume":"185","author":"Chen","year":"2001","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"830","DOI":"10.1111\/j.1471-0528.2005.00558.x","article-title":"The effect of prepartum antibiotics on the type of neonatal bacteraemia: Insights from the MRC ORACLE trials","volume":"112","author":"Gilbert","year":"2005","journal-title":"BJOG"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1007\/s40272-021-00465-z","article-title":"Potential Antibiotics for the Treatment of Neonatal Sepsis Caused by Multidrug-Resistant Bacteria","volume":"23","author":"Darlow","year":"2021","journal-title":"Paediatr. Drugs"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Pokhrel, B., Koirala, T., Shah, G., Joshi, S., and Baral, P. (2018). Bacteriological profile and antibiotic susceptibility of neonatal sepsis in neonatal intensive care unit of a tertiary hospital in Nepal. BMC Pediatr., 18.","DOI":"10.1186\/s12887-018-1176-x"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1055\/s-0035-1552935","article-title":"Reconsidering the Current Preterm Premature Rupture of Membranes Antibiotic Prophylactic Protocol","volume":"32","author":"Wolf","year":"2015","journal-title":"Am. J. Perinatol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"S11","DOI":"10.1086\/344654","article-title":"Bacterial resistance: Origins, epidemiology, and impact","volume":"36","author":"Livermore","year":"2003","journal-title":"Clin. Infect. Dis."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1093\/pch\/pxz073","article-title":"Early-onset neonatal sepsis: Organism patterns between 2009 and 2014","volume":"25","author":"Sgro","year":"2020","journal-title":"Paediatr. 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