{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T09:15:40Z","timestamp":1785575740799,"version":"3.56.0"},"reference-count":17,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2023,11,28]],"date-time":"2023-11-28T00:00:00Z","timestamp":1701129600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NOUCOR\/Uriach Group","award":["RINOPAF-1"],"award-info":[{"award-number":["RINOPAF-1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Background: The Platelet-Activating Factor (PAF)\/receptor (PAFR) system is involved in asthma and allergic rhinitis; however, its role in chronic rhinosinusitis (CRS) is still unclear. This study aimed to assess the expression of PAFR and the concentration of Lyso-PAF isoforms in the nasal polyps (NP) of patients suffering from CRS with\/without comorbidities such as asthma and NSAID-exacerbated respiratory disease (N-ERD) compared to healthy nasal mucosa (NM) from control subjects. Methods: NM (n = 8) and NP tissues were obtained from patients undergoing surgery for septal deviation\/turbinate hypertrophy or endoscopic sinus surgery, respectively. Three phenotypes were studied: CRSwNP with no asthma (n = 6), CRSwNP with non-steroidal anti-inflammatory drug (NSAID)-tolerant asthma (n = 6), and CRSwNP with NSAID-exacerbated respiratory disease (n = 6). PAFR protein and mRNA were assessed via immunochemistry, immunofluorescence, Western blot, and real-time quantitative PCR. Lyso-PAF isoforms (C16, C18, and C18:1) were quantified via mass spectrometry. Results: PAFR protein was expressed in the NM and NP, concretely in epithelial cells and submucosal glands. Compared to NM, PAFR mRNA expression was higher in all NP phenotypes (p &lt; 0.05) while all Lyso-PAF isoform concentrations were higher in the NP from asthmatic patients (p &lt; 0.05). Lyso-PAF C16 and C18 concentrations were higher in the NP from asthmatic patients than in the NP from patients without asthma. Conclusions: The PAF\/PAFR system could play a pathophysiological role in CRSwNP pathogenesis.<\/jats:p>","DOI":"10.3390\/jcm12237357","type":"journal-article","created":{"date-parts":[[2023,11,28]],"date-time":"2023-11-28T07:41:32Z","timestamp":1701157292000},"page":"7357","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Upregulation of Platelet-Activating Factor Receptor Expression and Lyso-Platelet-Activating Factor Isoforms in Human Nasal Polyp Tissues"],"prefix":"10.3390","volume":"12","author":[{"given":"Jordi","family":"Roca-Ferrer","sequence":"first","affiliation":[{"name":"Clinical and Experimental Respiratory Immunoallergy (IRCE), Clinic Foundation for Biomedical Research-August Pi Sunyer Biomedical Research Institute (FRCB-IDIBAPS), 08036 Barcelona, Spain"},{"name":"CIBER of Respiratory Diseases (CIBERES), Health Institute Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"P\u00e9rez-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Clinical and Experimental Respiratory Immunoallergy (IRCE), Clinic Foundation for Biomedical Research-August Pi Sunyer Biomedical Research Institute (FRCB-IDIBAPS), 08036 Barcelona, Spain"},{"name":"CIBER of Respiratory Diseases (CIBERES), Health Institute Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isam","family":"Alobid","sequence":"additional","affiliation":[{"name":"Clinical and Experimental Respiratory Immunoallergy (IRCE), Clinic Foundation for Biomedical Research-August Pi Sunyer Biomedical Research Institute (FRCB-IDIBAPS), 08036 Barcelona, Spain"},{"name":"CIBER of Respiratory Diseases (CIBERES), Health Institute Carlos III, 28029 Madrid, Spain"},{"name":"Rhinology Unit & Smell Clinic, ENT Department, Hospital Cl\u00ednic Barcelona, 08036 Barcelona, Spain"},{"name":"Faculty of Medicine, Universitat de Barcelona, 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeria","family":"Tubita","sequence":"additional","affiliation":[{"name":"Clinical and Experimental Respiratory Immunoallergy (IRCE), Clinic Foundation for Biomedical Research-August Pi Sunyer Biomedical Research Institute (FRCB-IDIBAPS), 08036 Barcelona, Spain"},{"name":"CIBER of Respiratory Diseases (CIBERES), Health Institute Carlos III, 28029 Madrid, Spain"},{"name":"Faculty of Medicine, Universitat de Barcelona, 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mireya","family":"Fuentes","sequence":"additional","affiliation":[{"name":"Clinical and Experimental Respiratory Immunoallergy (IRCE), Clinic Foundation for Biomedical Research-August Pi Sunyer Biomedical Research Institute (FRCB-IDIBAPS), 08036 Barcelona, Spain"},{"name":"CIBER of Respiratory Diseases (CIBERES), Health Institute Carlos III, 28029 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Bantul\u00e0","sequence":"additional","affiliation":[{"name":"Clinical and Experimental Respiratory Immunoallergy (IRCE), Clinic Foundation for Biomedical Research-August Pi Sunyer Biomedical Research Institute (FRCB-IDIBAPS), 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8566-8285","authenticated-orcid":false,"given":"Rosa","family":"Mu\u00f1oz-Cano","sequence":"additional","affiliation":[{"name":"Clinical and Experimental Respiratory Immunoallergy (IRCE), Clinic Foundation for Biomedical Research-August Pi Sunyer Biomedical Research Institute (FRCB-IDIBAPS), 08036 Barcelona, Spain"},{"name":"Faculty of Medicine, Universitat de Barcelona, 08036 Barcelona, Spain"},{"name":"Allergy Department, Hospital Cl\u00ednic, Universitat de Barcelona, 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5543-0345","authenticated-orcid":false,"given":"Antonio","family":"Valero","sequence":"additional","affiliation":[{"name":"Clinical and Experimental Respiratory Immunoallergy (IRCE), Clinic Foundation for Biomedical Research-August Pi Sunyer Biomedical Research Institute (FRCB-IDIBAPS), 08036 Barcelona, Spain"},{"name":"CIBER of Respiratory Diseases (CIBERES), Health Institute Carlos III, 28029 Madrid, Spain"},{"name":"Allergy Department, Hospital Cl\u00ednic, Universitat de Barcelona, 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I\u00f1aki","family":"Izquierdo","sequence":"additional","affiliation":[{"name":"Clinical Development & Medical Advise, R&D, NOUCOR, 08184 Palau Solit\u00e0 i Plegamans, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3463-5007","authenticated-orcid":false,"given":"Joaquim","family":"Mullol","sequence":"additional","affiliation":[{"name":"Clinical and Experimental Respiratory Immunoallergy (IRCE), Clinic Foundation for Biomedical Research-August Pi Sunyer Biomedical Research Institute (FRCB-IDIBAPS), 08036 Barcelona, Spain"},{"name":"CIBER of Respiratory Diseases (CIBERES), Health Institute Carlos III, 28029 Madrid, Spain"},{"name":"Rhinology Unit & Smell Clinic, ENT Department, Hospital Cl\u00ednic Barcelona, 08036 Barcelona, Spain"},{"name":"Faculty of Medicine, Universitat de Barcelona, 08036 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Mu\u00f1oz-Cano, R.M., Casas-Saucedo, R., Valero Santiago, A., Bobolea, I., Rib\u00f3, P., and 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