{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T14:51:32Z","timestamp":1787496692997,"version":"build-2736575974"},"reference-count":53,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2023,12,7]],"date-time":"2023-12-07T00:00:00Z","timestamp":1701907200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Background: Fumigation with hydrogen peroxide has proven to be a highly effective approach to maintaining biological safety within dental offices. The main purpose of this research was to investigate the efficacy of hydrogen peroxide (H2O2) fumigation in reducing bacterial levels in dental office environments. Methods: The study involved 30 participants diagnosed with moderate caries decay (ICDAS 3 and 4) in their mandibular molars. Sixty Petri dishes (two per patient) with Columbia Agar and 5% Sheep Blood were opened at the beginning of the caries treatment. After the completion of caries treatment and tooth restoration, 30 plates (G1 group) were closed. Following this, a 20 min fumigation procedure with 6% hydrogen peroxide biosanitizer using a compressed air device was conducted. After the fumigation, the remaining plates were closed (G2 group). The total number of bacteria CFUs (colony-forming units) in the dental office air was determined using the Koch sedimentation method. Results: The total bacterial colony (TBC) level, measured in cfu\/m3, demonstrated a significant decrease in the number of bacteria following room environment fumigation (163.1 \u00b1 145.7; G2 group) compared to non-fumigated samples (817.2 \u00b1 208.2; G1 group) (p &lt; 0.001). The predominant bacteria observed in the microbiological plates before fumigation were Micrococcus and Bacillus species, found in 80% (24\/30) and 60% (18\/30) of the plates, respectively. Application of H2O2 room fumigation resulted in a significant reduction in bacterial numbers: 79.2% (5\/30) for Micrococcus species (p &lt; 0.001), 83.3% (3\/30) for Bacillus species (p &lt; 0.001), and 100% (0\/30) for Staphylococcus arlettae (p &lt; 0.05). Conclusion: Fumigation with 6% H2O2 is an effective method for reducing bacterial counts in a dental office environment.<\/jats:p>","DOI":"10.3390\/jcm12247551","type":"journal-article","created":{"date-parts":[[2023,12,7]],"date-time":"2023-12-07T05:57:08Z","timestamp":1701928628000},"page":"7551","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The Impact of Hydrogen Peroxide (H2O2) Fumigation on Bacterial Levels in Dental Office Environments: A Randomized Clinical Trial Investigation"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3801-0218","authenticated-orcid":false,"given":"Jacek","family":"Matys","sequence":"first","affiliation":[{"name":"Oral Surgery Department, Wroclaw Medical University, 50-425 Wroclaw, Poland"},{"name":"Department of Orthodontics, Technische Universitat Dresden, 01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomasz","family":"Gedrange","sequence":"additional","affiliation":[{"name":"Oral Surgery Department, Wroclaw Medical University, 50-425 Wroclaw, Poland"},{"name":"Department of Orthodontics, Technische Universitat Dresden, 01307 Dresden, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marzena","family":"Dominiak","sequence":"additional","affiliation":[{"name":"Oral Surgery Department, Wroclaw Medical University, 50-425 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5700-4577","authenticated-orcid":false,"given":"Kinga","family":"Grzech-Le\u015bniak","sequence":"additional","affiliation":[{"name":"Oral Surgery Department, Wroclaw Medical University, 50-425 Wroclaw, Poland"},{"name":"Department of Periodontics, School of Dentistry, Virginia Commonwealth University, Richmond, VA 23284, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.5114\/jos.2020.94168","article-title":"COVID-19 and Professional Dental Practice. 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