{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:56:55Z","timestamp":1790791015126,"version":"4.1.0"},"reference-count":71,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2024,2,2]],"date-time":"2024-02-02T00:00:00Z","timestamp":1706832000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>(1) Background: This study offers a biexponential model to estimate corneal endothelial cell decay (ECD) following preloaded \u201cendothelium-in\u201d Descemet membrane endothelial keratoplasty (DMEK) in Fuchs\u2019 endothelial corneal dystrophy (FECD) patients; (2) Methods: A total of 65 eyes undergoing DMEK alone or combined with cataract surgery were evaluated. The follow-up period was divided into an early phase (first 6 months) and a late phase (up to 36 months). Endothelial cell count (ECC) and endothelial cell loss (ECL) were analyzed; (3) Results: The half time of the ECD was 3.03 months for the early phase and 131.50 months for the late phase. The predicted time-lapse interval to reach 500 cells\/mm2 was 218 months (18.17 years), while the time-lapse interval to reach 250 cells\/mm2 was 349 months (29.08 years). There was no statistically significant difference between the ECL in DMEK combined with cataract extraction and DMEK alone at 24 months (p \u2265\u00a00.20). At the late phase, long-term ECL prediction revealed a lower ECC half time in patients undergoing DMEK combined with cataract surgery (98.05 months) than DMEK alone (250.32 months); (4) Conclusions: Based on the mathematical modeling, a predicted average half-life of a DMEK graft could reach 18 years in FECD. Moreover, combining cataract extraction with DMEK could result in excessive ECL in the long term.<\/jats:p>","DOI":"10.3390\/jcm13030877","type":"journal-article","created":{"date-parts":[[2024,2,2]],"date-time":"2024-02-02T09:42:32Z","timestamp":1706866952000},"page":"877","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Predicting Long-Term Endothelial Cell Loss after Preloaded Descemet Membrane Endothelial Keratoplasty in Fuchs\u2019 Endothelial Corneal Dystrophy: A Mathematical Model"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8274-2662","authenticated-orcid":false,"given":"Pietro","family":"Viola","sequence":"first","affiliation":[{"name":"Ophthalmology Unit, San Bortolo Hospital, 36100 Vicenza, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3155-3279","authenticated-orcid":false,"given":"Enrico","family":"Neri","sequence":"additional","affiliation":[{"name":"Ophthalmology Unit, San Bortolo Hospital, 36100 Vicenza, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tommaso","family":"Occhipinti","sequence":"additional","affiliation":[{"name":"Ophthalmology Unit, San Bortolo Hospital, 36100 Vicenza, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5186-068X","authenticated-orcid":false,"given":"Mohit","family":"Parekh","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Schepens Eye Research Institute of Mass Eye and Ear, Harvard Medical School, Boston, MA 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Cian","sequence":"additional","affiliation":[{"name":"Ophthalmology Unit, San Bortolo Hospital, 36100 Vicenza, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"Ponzin","sequence":"additional","affiliation":[{"name":"Fondazione Banca degli Occhi del Veneto Onlus, 30174 Venice, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2847-1691","authenticated-orcid":false,"given":"Antonio","family":"Moramarco","sequence":"additional","affiliation":[{"name":"Ophthalmology Unit, IRCCS, Azienda Ospedaliero-Universitaria, 40138 Bologna, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfonso","family":"Iovieno","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, University of British Columbia, Vancouver, BC V5Z 1L3, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,2]]},"reference":[{"key":"ref_1","first-page":"12","article-title":"Biomaterials for corneal endothelial cell culture and tissue engineering","volume":"16","author":"Parekh","year":"2021","journal-title":"J Tissue Eng."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"281","DOI":"10.3109\/13816810.2015.1045524","article-title":"The inheritance of corneal endothelial cell density","volume":"37","author":"Racz","year":"2016","journal-title":"Ophthalmic Genet."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1396","DOI":"10.1016\/S0161-6420(03)00463-9","article-title":"Long-term graft survival after penetrating keratoplasty","volume":"110","author":"Thompson","year":"2003","journal-title":"Ophthalmology"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/S0002-9394(14)72898-3","article-title":"Corneal endothelium five years after transplantation","volume":"118","author":"Bourne","year":"1994","journal-title":"Am. 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