{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T21:42:58Z","timestamp":1790286178628,"version":"4.1.0"},"reference-count":45,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2024,2,6]],"date-time":"2024-02-06T00:00:00Z","timestamp":1707177600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Background: High-Intensity Focused Ultrasound (HIFU) has emerged as a precise and non-invasive modality for tissue ablation and healing. This study presents a detailed dermoscopic analysis of skin healing post-High-Intensity Focused Ultrasound (HIFU) treatment, focusing on common benign skin lesions, such as seborrheic keratosis, sebaceous hyperplasia, vascular lesions, and sebaceous nevi. Methods: Prior to HIFU treatment, a comprehensive assessment was conducted, integrating ultrasound scanning and clinical evaluations. The TOOsonix System ONE-M was employed for HIFU treatments, with parameters tailored to each lesion type. Results: A common pattern observed across all lesions includes initial whitening post treatment, followed by scab formation and the development of a pink area with reparative vessels. This study, however, highlights distinct differences in fibrosis patterns and healing timelines across different lesion types. Each lesion type exhibited unique fibrosis patterns post treatment. Flatter variants of seborrheic keratosis healed within a month, displaying hypopigmentation and reparative vessels, alongside a distinct lattice fibrosis pattern in more verrucous forms, which took about two months to heal. Sebaceous hyperplasia, characterized by rapid healing within three weeks, demonstrated fibrosis with pink areas and perpendicular white lines, concluding with a slight depression. Vascular lesions varied in healing time based on depth, with superficial ones showing whitening and crust formation, while deeper lesions had vessel occlusion and size reduction accompanied by concentric fibrotic bands. Sebaceous nevi presented the longest healing duration of three months, characterized by amorphous white-gray structures, scab formation, and the emergence of pink areas with branching vessels, leading to clear skin with reduced white lines. Conclusions: in conclusion, this meticulous clinical evaluation highlights the unique healing characteristics and timelines for each skin lesion type treated with HIFU. These insights are invaluable for optimizing follow-up assessments, identifying potential complications, and refining treatment protocols. By providing detailed insights into the healing timelines and patterns for different types of lesions, patients can be better informed about their post-treatment journey.<\/jats:p>","DOI":"10.3390\/jcm13040931","type":"journal-article","created":{"date-parts":[[2024,2,6]],"date-time":"2024-02-06T12:38:24Z","timestamp":1707223104000},"page":"931","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Healing Process after High-Intensity Focused Ultrasound Treatment of Benign Skin Lesions: Dermoscopic Analysis and Treatment Guidelines"],"prefix":"10.3390","volume":"13","author":[{"given":"Jacek","family":"Calik","sequence":"first","affiliation":[{"name":"Department of Clinical Oncology, Wroclaw Medical University, 50-556 Wroc\u0142aw, Poland"},{"name":"Old Town Clinic, 50-136 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomasz","family":"Zawada","sequence":"additional","affiliation":[{"name":"TOOsonix A\/S, 2970 Hoersholm, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3918-1182","authenticated-orcid":false,"given":"Torsten","family":"Bove","sequence":"additional","affiliation":[{"name":"TOOsonix A\/S, 2970 Hoersholm, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piotr","family":"Dzi\u0119giel","sequence":"additional","affiliation":[{"name":"Division of Histology and Embryology, Department of Human Morphology and Embryology, Wroclaw Medical University, T. Chalubinskiego 6a, 50-368 Wroclaw, Poland"},{"name":"Department of Human Biology, Faculty of Physiotherapy, Wroclaw University of Health and Sport Sciences, 51-612 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Pogorzelska-Antkowiak","sequence":"additional","affiliation":[{"name":"EsteDerm Private Dermatology Clinic, 43-100 Tychy, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacek","family":"Mackiewicz","sequence":"additional","affiliation":[{"name":"Department of Medical and Experimental Oncology, Institute of Oncology, Poznan University of Medical Sciences, 61-701 Poznan, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bartosz","family":"Wo\u017aniak","sequence":"additional","affiliation":[{"name":"Old Town Clinic, 50-136 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1898-3952","authenticated-orcid":false,"given":"Natalia","family":"Sauer","sequence":"additional","affiliation":[{"name":"Old Town Clinic, 50-136 Wroclaw, Poland"},{"name":"Faculty of Pharmacy, Wroclaw Medical University, 50-556 Wroclaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1080\/02656730601186138","article-title":"High intensity focused ultrasound: Physical principles and devices","volume":"23","author":"Haar","year":"2007","journal-title":"Int. J. Hyperth."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1016\/j.ultrasmedbio.2022.03.002","article-title":"Strongly Focused HIFU Transducers with Simultaneous Optical Observation for Treatment of Skin at 20 MHz","volume":"48","author":"Zawada","year":"2022","journal-title":"Ultrasound Med. Biol."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Izadifar, Z., Izadifar, Z., Chapman, D., and Babyn, P. (2020). An Introduction to High Intensity Focused Ultrasound: Systematic Review on Principles, Devices, and Clinical Applications. J. Clin. Med., 9.","DOI":"10.3390\/jcm9020460"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1007\/s00262-016-1891-9","article-title":"Thermal and mechanical high-intensity focused ultrasound: Perspectives on tumor ablation, immune effects and combination strategies","volume":"66","author":"Eikelenboom","year":"2017","journal-title":"Cancer Immunol. Immunother."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1111\/srt.12239","article-title":"High intensity focused ultrasound as a potential new modality for the treatment of pigmentary skin disorder","volume":"22","author":"Choi","year":"2016","journal-title":"Ski. Res. Technol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1159\/000515075","article-title":"Removal of Common Warts by High-Intensity Focused Ultrasound: An Introductory Observation","volume":"13","author":"Bove","year":"2021","journal-title":"Case Rep. Dermatol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1159\/000525896","article-title":"Treatment of Condylomata Acuminata Using a New Non-Vapor-Generating Focused Ultrasound Method following Imiquimod 5% Cream","volume":"14","author":"Calik","year":"2022","journal-title":"Case Rep. Dermatol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1111\/srt.12661","article-title":"High-frequency (20-MHz) high-intensity focused ultrasound (HIFU) system for dermal intervention: Preclinical evaluation in skin equivalents","volume":"25","author":"Bove","year":"2019","journal-title":"Ski. Res. Technol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"145","DOI":"10.2147\/CCID.S348106","article-title":"Treatment of Seborrheic Keratosis by High Frequency Focused Ultrasound\u2014An Early Experience with 11 Consecutive Cases","volume":"15","author":"Calik","year":"2022","journal-title":"Clin. Cosmet. Investig. Dermatol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Imtiaz, C., Farooqi, M.A., Bhatti, T., Lee, J., Moin, R., Kang, C.U., and Farooqi, H.M.U. (2023). Focused Ultrasound, an Emerging Tool for Atherosclerosis Treatment: A Comprehensive Review. Life, 13.","DOI":"10.3390\/life13081783"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1055\/s-0035-1564793","article-title":"High-Intensity Focused Ultrasound: Current Status for Image-Guided Therapy","volume":"32","author":"Copelan","year":"2015","journal-title":"Semin. Intervent. Radiol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Zulkifli, D., Manan, H.A., Yahya, N., and Hamid, H.A. (2023). The Applications of High-Intensity Focused Ultrasound (HIFU) Ablative Therapy in the Treatment of Primary Breast Cancer: A Systematic Review. Diagnostics, 13.","DOI":"10.3390\/diagnostics13152595"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"302","DOI":"10.3109\/02656736.2014.969789","article-title":"Emerging HIFU applications in cancer therapy","volume":"31","author":"Maloney","year":"2015","journal-title":"Int. J. Hyperth."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"688","DOI":"10.5021\/ad.2015.27.6.688","article-title":"High-Intensity Focused Ultrasound for the Treatment of Wrinkles and Skin Laxity in Seven Different Facial Areas","volume":"27","author":"Park","year":"2015","journal-title":"Ann. Dermatol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1007\/s10103-020-02957-9","article-title":"Systematic review and meta-analysis of safety and efficacy of high-intensity focused ultrasound (HIFU) for face and neck rejuvenation","volume":"35","author":"Ayatollahi","year":"2020","journal-title":"Lasers Med. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1159\/000534270","article-title":"Treatment of Cutaneous Neurofibromas in Patients with Neurofibromatosis Type 1","volume":"15","author":"Wozniak","year":"2023","journal-title":"Case Rep. Dermatol."},{"key":"ref_17","first-page":"138","article-title":"Effects of highfrequency ultrasound treatment on human skin tissues","volume":"6","author":"Bani","year":"2014","journal-title":"Surg. Cosmet. Dermatol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1111\/srt.12347","article-title":"High-intensity focused ultrasound treatment for skin: Ex vivo evaluation","volume":"23","author":"Park","year":"2017","journal-title":"Ski. Res. Technol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1007\/s10103-017-2393-5","article-title":"Non-invasive high-intensity focused ultrasound for UV-induced hyperpigmentation in Fitzpatrick skin types III and IV: A prospective, randomized, controlled, evaluator-blinded trial","volume":"33","author":"Vachiramon","year":"2018","journal-title":"Lasers Med. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e15420","DOI":"10.1111\/dth.15420","article-title":"Safety and efficacy of high-intensity focused ultrasound for treatment of periorbital, perioral, and neck wrinkles: Prospective open single-center single-arm confirmatory clinical trial","volume":"35","author":"Han","year":"2022","journal-title":"Dermatol. Ther."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"130","DOI":"10.5114\/dr.2022.117984","article-title":"High-intensity focused ultrasound technology as a non-surgical alternative to face lifting","volume":"109","author":"Smoczok","year":"2022","journal-title":"Dermatol. Rev. Dermatologiczny"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1111\/jocd.13044","article-title":"A study of efficacy and safety of high-intensity focused ultrasound for the treatment of melasma in Asians: A single-blinded, randomized, split-face, pilot study","volume":"19","author":"Vachiramon","year":"2020","journal-title":"J. Cosmet. Dermatol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1111\/srt.12883","article-title":"High-frequency (20 MHz) high-intensity focused ultrasound: New Treatment of actinic keratosis, basal cell carcinoma, and Kaposi sarcoma. An open-label exploratory study","volume":"26","author":"Serup","year":"2020","journal-title":"Ski. Res. Technol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3859","DOI":"10.1016\/j.bjps.2022.06.073","article-title":"Rejuvenation of photoaged aged mouse skin using high-intensity focused ultrasound","volume":"75","author":"Lee","year":"2022","journal-title":"J. Plast. Reconstr. Aesthetic Surg."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"489","DOI":"10.2147\/CCID.S350556","article-title":"Quantified Facial Rejuvenation Utilizing High Intense Focus Ultrasound with Multiple Penetrative Depths","volume":"15","author":"Lio","year":"2022","journal-title":"Clin. Cosmet. Investig. Dermatol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1111\/jocd.13008","article-title":"Efficacy of high-intensity focused ultrasound in facial and neck rejuvenation","volume":"19","author":"Serdar","year":"2020","journal-title":"J. Cosmet. Dermatol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1111\/srt.12371","article-title":"Efficacy and safety of non-invasive body tightening with high-intensity focused ultrasound (HIFU)","volume":"23","author":"Ko","year":"2017","journal-title":"Ski. Res. Technol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1097\/PRS.0000000000009912","article-title":"Effectiveness of HIFU Therapy for Nonsurgical Facial and Body Contouring: A Systematic Review of Prospective and Experimental Studies","volume":"151","author":"Kumar","year":"2023","journal-title":"Plast. Reconstr. Surg."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1111\/j.1524-4725.2011.02148.x","article-title":"A Randomized, Single-Blind, Postmarketing Study of Multiple Energy Levels of High-Intensity Focused Ultrasound for Noninvasive Body Sculpting","volume":"38","author":"Nowell","year":"2012","journal-title":"Dermatol. Surg."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Liu, Y., Yin, Q., Xu, F., and Luo, S. (2022). Clinical efficacy and safety of high-intensity focused ultrasound (HIFU) ablation in treatment of cesarean scar pregnancy (CSP) I and II. BMC Pregnancy Childbirth, 22.","DOI":"10.1186\/s12884-022-04848-z"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"395","DOI":"10.2147\/CCID.S267246","article-title":"Differential Diagnosis and Management on Seborrheic Keratosis in Elderly Patients","volume":"14","author":"Moscarella","year":"2021","journal-title":"Clin. Cosmet. Investig. Dermatol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1556","DOI":"10.1001\/archderm.138.12.1556","article-title":"Dermoscopy of Pigmented Seborrheic Keratosis: A Morphological Study","volume":"138","author":"Braun","year":"2002","journal-title":"Arch. Dermatol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1111\/jdv.12241","article-title":"Evaluation of dermoscopic algorithm for seborrhoeic keratosis: A prospective study in 412 patients","volume":"28","author":"Lin","year":"2014","journal-title":"J. Eur. Acad. Dermatol. Venereol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1016\/j.ad.2018.10.014","article-title":"Dermoscopic Analysis of 72 \u2018Atypical\u2019 Seborrheic Keratoses","volume":"110","author":"Mazzeo","year":"2019","journal-title":"Actas Dermo-Sifiliogr\u00e1ficas"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1016\/j.jaad.2006.08.028","article-title":"\u2018Fat fingers:\u2019 A clue in the dermoscopic diagnosis of seborrheic keratoses","volume":"55","author":"Kopf","year":"2006","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1111\/j.1365-2230.2004.1612.x","article-title":"Dermatoscopic features of benign sebaceous proliferation","volume":"29","author":"Bryden","year":"2004","journal-title":"Clin. Exp. Dermatol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"808","DOI":"10.1001\/archderm.141.6.808","article-title":"Dermoscopy of sebaceous hyperplasia","volume":"141","author":"Zaballos","year":"2005","journal-title":"Arch. Dermatol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1111\/j.1468-3083.2008.02827.x","article-title":"Bonbon toffee sign: A new dermatoscopic feature for sebaceous hyperplasia","volume":"22","author":"Oztas","year":"2008","journal-title":"J. Eur. Acad. Dermatol. Venereol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.jaad.2013.09.018","article-title":"Partially involuting congenital hemangiomas: A report of 8 cases and review of the literature","volume":"70","author":"Nasseri","year":"2014","journal-title":"J. Am. Acad. Dermatol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/S0140-6736(16)00645-0","article-title":"Infantile haemangioma","volume":"390","author":"Harper","year":"2017","journal-title":"Lancet"},{"key":"ref_41","first-page":"e26292","article-title":"Dermoscopic Analysis of Vascular Malformations and Tumors Based Upon Dominant Vascular Dermoscopic Features: A Retrospective Analysis From a Tertiary Care Center of East India","volume":"14","author":"Viswan","year":"2022","journal-title":"Cureus"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"692060","DOI":"10.3389\/fmed.2021.692060","article-title":"Dermoscopic Features Summarization and Comparison of Four Types of Cutaneous Vascular Anomalies","volume":"8","author":"Gao","year":"2021","journal-title":"Front. Med."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"3371","DOI":"10.1111\/jocd.14682","article-title":"Treatment of superficial benign vascular tumors by high intensity focused ultrasound: Observations in two illustrative cases","volume":"21","author":"Calik","year":"2022","journal-title":"J. Cosmet. Dermatol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Sandhu, S., Neema, S., Singh, P.Y., Dangwal, V., and Kothari, R. (2023). Dermoscopy of nevus sebaceus: A cross-sectional study of 22 cases. Indian J. Dermatol. Venereol. Leprol., 1\u20136.","DOI":"10.25259\/IJDVL_629_2022"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1159\/000460258","article-title":"Dermoscopic Analysis of Nevus Sebaceus of Jadassohn: A Study of 13 Cases","volume":"3","author":"Kelati","year":"2017","journal-title":"Ski. Appendage Disord."}],"container-title":["Journal of Clinical Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2077-0383\/13\/4\/931\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T13:55:54Z","timestamp":1760104554000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2077-0383\/13\/4\/931"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,6]]},"references-count":45,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2024,2]]}},"alternative-id":["jcm13040931"],"URL":"https:\/\/doi.org\/10.3390\/jcm13040931","relation":{},"ISSN":["2077-0383"],"issn-type":[{"value":"2077-0383","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,6]]}}}