{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T01:47:05Z","timestamp":1789696025473,"version":"4.0.1"},"reference-count":33,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T00:00:00Z","timestamp":1718323200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Background\/Objectives: Gestational diabetes (GDM) is a metabolic disorder with altered glucose levels diagnosed in pregnant women. The pathogenesis of GDM is not fully known, but it is thought to be caused by impaired insulin production and insulin resistance induced by diabetogenic factors. The placenta may play an important role in the development of GDM. Glucose transporters (GLUTs) are responsible for the delivery of glucose into the foetal circulation. Placental zinc transporters regulate insulin and glucagon secretion, as well as gluconeogenesis and glycolysis. The aim of this study was to investigate the placental expression of GLUT3, GLUT4, GLUT7 and SLC30A8 in women with GDM. Furthermore, we evaluated whether the expression profiles of these transporters were correlated with clinical parameters. Methods: This study included 26 patients with GDM and 28 patients with normal glucose tolerance (NGT). Results: The placental expression of GLUT3 was significantly reduced in the GDM group, while the placental expression of GLUT4, GLUT7 and SLC30A8 was significantly upregulated in the GDM group. GLUT3 expression correlated significantly with body mass index (BMI) increase during pregnancy and body mass increase during pregnancy, while GLUT4 expression correlated negatively with BMI at birth. Conclusions: These results suggest the involvement of GLUT3 and GLUT4, GLUT7 and SLC30A8 in the pathogenesis of GDM.<\/jats:p>","DOI":"10.3390\/jcm13123500","type":"journal-article","created":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T06:29:43Z","timestamp":1718605783000},"page":"3500","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Placental Expression of Glucose and Zinc Transporters in Women with Gestational Diabetes"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4754-3235","authenticated-orcid":false,"given":"\u0141ukasz","family":"Ustianowski","sequence":"first","affiliation":[{"name":"Department of Physiology, Pomeranian Medical University, 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micha\u0142","family":"Czerewaty","sequence":"additional","affiliation":[{"name":"Department of Physiology, Pomeranian Medical University, 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8227-6753","authenticated-orcid":false,"given":"Kajetan","family":"Kie\u0142bowski","sequence":"additional","affiliation":[{"name":"Department of Physiology, Pomeranian Medical University, 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estera","family":"Bakinowska","sequence":"additional","affiliation":[{"name":"Department of Physiology, Pomeranian Medical University, 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3869-5077","authenticated-orcid":false,"given":"Maciej","family":"Tarnowski","sequence":"additional","affiliation":[{"name":"Department of Physiology in Health Sciences, Pomeranian Medical University, 70-210 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9415-2758","authenticated-orcid":false,"given":"Krzysztof","family":"Safranow","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6557-1208","authenticated-orcid":false,"given":"Andrzej","family":"Pawlik","sequence":"additional","affiliation":[{"name":"Department of Physiology, Pomeranian Medical University, 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"109050","DOI":"10.1016\/j.diabres.2021.109050","article-title":"IDF Diabetes Atlas: Estimation of Global and Regional Gestational Diabetes Mellitus Prevalence for 2021 by International Association of Diabetes in Pregnancy Study Group\u2019s Criteria","volume":"183","author":"Wang","year":"2022","journal-title":"Diabetes Res. Clin. Pract."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"636","DOI":"10.3389\/fendo.2020.00636","article-title":"Incidence and Risk Factors of Gestational Diabetes Mellitus: A Prospective Cohort Study in Qingdao, China","volume":"11","author":"Li","year":"2020","journal-title":"Front. Endocrinol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1111\/ahg.12356","article-title":"The role of genetics and epigenetics in the pathogenesis of gestational diabetes mellitus","volume":"84","author":"Rosik","year":"2020","journal-title":"Ann. Hum. Genet."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3","DOI":"10.4093\/dmj.2021.0335","article-title":"Gestational Diabetes Mellitus: Diagnostic Approaches and Maternal-Offspring Complications","volume":"46","author":"Moon","year":"2022","journal-title":"Diabetes Metab. J."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.ogc.2017.02.002","article-title":"Gestational Diabetes: Diagnosis, Classification, and Clinical Care","volume":"44","author":"Mack","year":"2017","journal-title":"Obstet. Gynecol. Clin. N. Am."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.mam.2012.07.001","article-title":"The SLC2 (GLUT) family of membrane transporters","volume":"34","author":"Mueckler","year":"2013","journal-title":"Mol. Aspects Med."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Zhang, L., Yu, X., Wu, Y., Fu, H., Xu, P., Zheng, Y., Wen, L., Yang, X., Zhang, F., and Hu, M. (2021). Gestational Diabetes Mellitus-Associated Hyperglycemia Impairs Glucose Transporter 3 Trafficking in Trophoblasts Through the Downregulation of AMP-Activated Protein Kinase. Front. Cell Dev. Biol., 9.","DOI":"10.3389\/fcell.2021.722024"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.placenta.2020.11.008","article-title":"Decreased maternal serum adiponectin and increased insulin-like growth factor-1 levels along with increased placental glucose transporter-1 expression in gestational diabetes mellitus: Possible role in fetal overgrowth","volume":"104","author":"Balachandiran","year":"2021","journal-title":"Placenta"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1079\/PNS2003320","article-title":"Intestinal and placental zinc transport pathways","volume":"63","author":"Ford","year":"2004","journal-title":"Proc. Nutr. Soc."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/s12576-017-0571-7","article-title":"Zinc status is associated with inflammation, oxidative stress, lipid, and glucose metabolism","volume":"68","author":"Olechnowicz","year":"2018","journal-title":"J. Physiol. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2140","DOI":"10.1080\/14767058.2019.1659769","article-title":"The influence of zinc supplementation on metabolic status in gestational diabetes: A meta-analysis of randomized controlled studies","volume":"34","author":"Li","year":"2021","journal-title":"J. Matern. Fetal Neonatal Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1159714","DOI":"10.3389\/fendo.2023.1159714","article-title":"Association of solute carrier family 30 A8 zinc transporter gene variations with gestational diabetes mellitus risk in a Chinese population","volume":"14","author":"Zeng","year":"2023","journal-title":"Front. Endocrinol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1007\/s12020-018-1651-z","article-title":"Association of type 2 diabetes susceptible genes GCKR, SLC30A8, and FTO polymorphisms with gestational diabetes mellitus risk: A meta-analysis","volume":"62","author":"Lin","year":"2018","journal-title":"Endocrine"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"676","DOI":"10.2337\/dc10-0719","article-title":"International association of diabetes and pregnancy study groups recommendations on the diagnosis and classification of hyperglycemia in pregnancy","volume":"33","author":"Metzger","year":"2010","journal-title":"Diabetes Care"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Ustianowski, P., Malinowski, D., Kopytko, P., Czerewaty, M., Tarnowski, M., Dziedziejko, V., Safranow, K., and Pawlik, A. (2021). ADCY5, CAPN10 and JAZF1 Gene Polymorphisms and Placental Expression in Women with Gestational Diabetes. Life, 11.","DOI":"10.3390\/life11080806"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Fajardy, I., Moitrot, E., Vambergue, A., Vandersippe-Millot, M., Deruelle, P., and Rousseaux, J. (2009). Time course analysis of RNA stability in human placenta. BMC Mol. Biol., 10.","DOI":"10.1186\/1471-2199-10-21"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1016\/j.placenta.2004.09.009","article-title":"Evaluation of housekeeping genes in placental comparative expression studies","volume":"26","author":"Meller","year":"2005","journal-title":"Placenta"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2053","DOI":"10.1093\/hmg\/ddab169","article-title":"Prenatal inflammation as a link between placental expression signature of tryptophan metabolism and preterm birth","volume":"30","author":"Karahoda","year":"2021","journal-title":"Hum. Mol. Genet."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1041","DOI":"10.1016\/j.placenta.2011.09.014","article-title":"Glucose transporter 3 (GLUT3) protein expression in human placenta across gestation","volume":"32","author":"Brown","year":"2011","journal-title":"Placenta"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1016\/j.placenta.2013.08.010","article-title":"Placental glucose transporter 3 (GLUT3) is up-regulated in human pregnancies complicated by late-onset intrauterine growth restriction","volume":"34","author":"Janzen","year":"2013","journal-title":"Placenta"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Aldahmash, W., Harrath, A.H., Aljerian, K., Sabr, Y., and Alwasel, S. (2023). Expression of Glucose Transporters 1 and 3 in the Placenta of Pregnant Women with Gestational Diabetes Mellitus. Life, 13.","DOI":"10.3390\/life13040993"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.diabres.2016.07.003","article-title":"Expression and correlation of sex hormone-binding globulin and insulin signal transduction and glucose transporter proteins in gestational diabetes mellitus placental tissue","volume":"119","author":"Zhang","year":"2016","journal-title":"Diabetes Res. Clin. Pract."},{"key":"ref_23","first-page":"258","article-title":"Is fetal macrosomia in adequately controlled diabetic women the result of a placental defect?--a hypothesis","volume":"11","author":"Desoye","year":"2002","journal-title":"J. Matern. Fetal Neonatal Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1172\/JCI118036","article-title":"Overexpression of GLUT3 placental glucose transporter in diabetic rats","volume":"96","author":"Boileau","year":"1995","journal-title":"J. Clin. Investig."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1111\/jdi.13680","article-title":"Placental expression of glucose transporters GLUT-1, GLUT-3, GLUT-8 and GLUT-12 in pregnancies complicated by gestational and type 1 diabetes mellitus","volume":"13","author":"Stanirowski","year":"2022","journal-title":"J. Diabetes Investig."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Stanirowski, P.J., Szukiewicz, D., Majewska, A., W\u0105troba, M., Pyzlak, M., Bomba-Opo\u0144, D., and Wielgo\u015b, M. (2021). Differential Expression of Glucose Transporter Proteins GLUT-1, GLUT-3, GLUT-8 and GLUT-12 in the Placenta of Macrosomic, Small-for-Gestational-Age and Growth-Restricted Foetuses. J. Clin. Med., 10.","DOI":"10.3390\/jcm10245833"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/j.jcjd.2017.04.008","article-title":"Placental Expression of Glucose Transporter Proteins in Pregnancies Complicated by Gestational and Pregestational Diabetes Mellitus","volume":"42","author":"Stanirowski","year":"2018","journal-title":"Can. J. Diabetes"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1007\/s12020-016-1202-4","article-title":"Impact of pre-gestational and gestational diabetes mellitus on the expression of glucose transporters GLUT-1, GLUT-4 and GLUT-9 in human term placenta","volume":"55","author":"Stanirowski","year":"2017","journal-title":"Endocrine"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Mohammed, S., Qadri, S.S.Y.H., Molangiri, A., Basak, S., and Rajkumar, H. (2023). Gestational low dietary protein induces intrauterine inflammation and alters the programming of adiposity and insulin sensitivity in the adult offspring. J. Nutr. Biochem., 116.","DOI":"10.1016\/j.jnutbio.2023.109330"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Lipka, A., Paukszto, \u0141., Kennedy, V.C., Tanner, A.R., Majewska, M., and Anthony, R.V. (2024). The Impact of SLC2A8 RNA Interference on Glucose Uptake and the Transcriptome of Human Trophoblast Cells. Cells, 13.","DOI":"10.3390\/cells13050391"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e23610","DOI":"10.1096\/fj.202301700RR","article-title":"Multiple genetic variants at the SLC30A8 locus affect local super-enhancer activity and influence pancreatic \u03b2-cell survival and function","volume":"38","author":"Hu","year":"2024","journal-title":"FASEB J."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2203","DOI":"10.1007\/s10528-023-10385-x","article-title":"Association of HHEX and SLC30A8 Gene Polymorphisms with Gestational Diabetes Mellitus Susceptibility: A Meta-analysis","volume":"61","author":"Xie","year":"2023","journal-title":"Biochem. Genet."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1111\/dme.12629","article-title":"Zinc transporter 8 autoantibodies: What is their clinical relevance in gestational diabetes?","volume":"32","author":"Rudland","year":"2015","journal-title":"Diabet. Med."}],"container-title":["Journal of Clinical Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2077-0383\/13\/12\/3500\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:59:09Z","timestamp":1760108349000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2077-0383\/13\/12\/3500"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,14]]},"references-count":33,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2024,6]]}},"alternative-id":["jcm13123500"],"URL":"https:\/\/doi.org\/10.3390\/jcm13123500","relation":{},"ISSN":["2077-0383"],"issn-type":[{"value":"2077-0383","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,14]]}}}