{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:17:47Z","timestamp":1760145467134,"version":"build-2065373602"},"reference-count":44,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2024,7,28]],"date-time":"2024-07-28T00:00:00Z","timestamp":1722124800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Interdisciplinary Centre for Clinical Research (IZKF) Erlangen","award":["SFB 643 B10","FOR 2240","SFB 1607"],"award-info":[{"award-number":["SFB 643 B10","FOR 2240","SFB 1607"]}]},{"name":"ELAN Fonds","award":["SFB 643 B10","FOR 2240","SFB 1607"],"award-info":[{"award-number":["SFB 643 B10","FOR 2240","SFB 1607"]}]},{"name":"DFG","award":["SFB 643 B10","FOR 2240","SFB 1607"],"award-info":[{"award-number":["SFB 643 B10","FOR 2240","SFB 1607"]}]},{"name":"EU Restore Vision","award":["SFB 643 B10","FOR 2240","SFB 1607"],"award-info":[{"award-number":["SFB 643 B10","FOR 2240","SFB 1607"]}]},{"name":"Jerini AG, Berlin","award":["SFB 643 B10","FOR 2240","SFB 1607"],"award-info":[{"award-number":["SFB 643 B10","FOR 2240","SFB 1607"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Background: Corneal inflammatory hem- and lymphangiogenesis significantly increase the risk for immune rejection after subsequent allogeneic corneal transplantation. The purpose of this study was to analyze the impact of temporary selective inhibition of lymphangiogenesis after transplantation on graft survival. Methods: Allogeneic transplantation from C57BL\/6 mice to BalbC mice was performed as \u201chigh-risk\u201d keratoplasty in a prevascularized corneal host bed (suture-induced inflammatory corneal neovascularization). The treatment group received integrin \u03b15\u03b21-blocking small molecules (JSM6427) at the time of transplantation and for two weeks afterwards. Control mice received a vehicle solution. Grafts were evaluated weekly for graft rejection using an opacity score. At the end of the follow-up, immunohistochemical staining of corneal wholemounts for lymphatic vessels as well as CD11b+ immune cells was performed. Results: Temporary postoperative inhibition of lymphangiogenesis by JSM6427 improved the corneal graft survival significantly. At the end of the follow-up, no significant reduction in CD11b+ immunoreactive cells within the graft compared to controls was found. Conclusions: The significant improvement of corneal graft survival by the selective, temporary postoperative inhibition of lymphangiogenesis after keratoplasty using integrin antagonists shows the impact of lymphatic vessels in the early postoperative phase. Retarding lymphatic vessel ingrowth into the graft might be sufficient for the shift to immunological tolerance in the postoperative period, even after high-risk keratoplasty.<\/jats:p>","DOI":"10.3390\/jcm13154418","type":"journal-article","created":{"date-parts":[[2024,7,29]],"date-time":"2024-07-29T09:50:05Z","timestamp":1722246605000},"page":"4418","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Selective, Temporary Postoperative Inhibition of Lymphangiogenesis by Integrin \u03b15\u03b21 Blockade Improves Allograft Survival in a Murine Model of High-Risk Corneal Transplantation"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9041-2249","authenticated-orcid":false,"given":"Tina","family":"Dietrich-Ntoukas","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin and Humboldt Universit\u00e4t zu Berlin, 13353 Berlin, Germany"},{"name":"Department of Ophthalmology, University Erlangen-N\u00fcrnberg, 91054 Erlangen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felix","family":"Bock","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, University Hospital of Cologne, 50937 Cologne, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jasmine","family":"Onderka","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, University Erlangen-N\u00fcrnberg, 91054 Erlangen, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deniz","family":"Hos","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, University Hospital of Cologne, 50937 Cologne, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bjoern O.","family":"Bachmann","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, University Hospital of Cologne, 50937 Cologne, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grit","family":"Zahn","sequence":"additional","affiliation":[{"name":"Eternygen GmbH, 10178 Berlin, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1958-411X","authenticated-orcid":false,"given":"Claus","family":"Cursiefen","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, University Hospital of Cologne, 50937 Cologne, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1007\/s00417-002-0496-5","article-title":"Risk factors for corneal allograft rejection: Intermediate results of a prospective normal-risk keratoplasty study","volume":"240","author":"Kuchle","year":"2002","journal-title":"Graefes Arch. 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