{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T15:22:00Z","timestamp":1787498520750,"version":"build-2736575974"},"reference-count":10,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T00:00:00Z","timestamp":1725926400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Background: In EDTA-induced pseudothrombopenia, citrate or MgSO4 are recommended for platelet counting. Pre-analytical conditions are poorly defined for tubes containing MgSO4 or citrate. In this study, we analyzed the impact of agitation of these tubes on platelet counts. Methods: K2EDTA, citrate, and MgSO4 tubes from 70 patients were gently agitated on a wheel rotating at 20 rpm. Platelets were analyzed on the Sysmex XN analyzer at different times, and the percentage of platelet deviation from T0 was assessed and compared with the desirable bias of the EFLM. Results: at 180 min in fluorescence, the relative variation of platelets after shaking is 1.17% for K2EDTA, \u221229.76% for citrate, and \u221233.18% for MgSO4, while for unshaken MgSO4 platelets the variation is \u22121.3%. The reduction in platelet numbers when citrate or MgSO4 tubes are shaken is linked to the appearance of platelet clusters. Conclusions: agitation of MgSO4 and especially citrate tubes led to a decrease in platelet counts due to the formation of platelet aggregates; on the other hand, platelet counts on EDTA are virtually stable. During transport, we recommend putting sodium citrate and MgSO4 tubes in an upright position and avoiding shaking them to avoid giving an erroneous platelet result.<\/jats:p>","DOI":"10.3390\/jcm13185350","type":"journal-article","created":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T05:53:03Z","timestamp":1725947583000},"page":"5350","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Impact of Shaking EDTA, Citrate, or MgSO4 Tubes on Platelet Count Results"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6349-9895","authenticated-orcid":false,"given":"Michel","family":"Soulard","sequence":"first","affiliation":[{"name":"Biogroup Hematology Laboratory, La Chocolaterie, 92300 Levallois Perret, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hela","family":"Ketatni","sequence":"additional","affiliation":[{"name":"Biogroup Hematology Laboratory, La Chocolaterie, 92300 Levallois Perret, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Visseaux","sequence":"additional","affiliation":[{"name":"Biogroup Hematology Laboratory, La Chocolaterie, 92300 Levallois Perret, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascale","family":"Croix","sequence":"additional","affiliation":[{"name":"Biogroup Hematology Laboratory, La Chocolaterie, 92300 Levallois Perret, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9624-2172","authenticated-orcid":false,"given":"Patrick","family":"Cohen","sequence":"additional","affiliation":[{"name":"Division of Laboratory Medecine, Diagnostic Department, Geneva University Hospitals and Faculty of Medicine, 1205 Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Lardinois, B., Favresse, J., Chatelain, B., Lippi, G., and Mullier, F. (2021). Pseudothrombocytopenia\u2014A review on causes, occurrence and clinical implications. J. Clin. Med., 10.","DOI":"10.3390\/jcm10040594"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Baccini, V., Genevi\u00e8ve, F., Jacqmin, H., Chatelain, B., Girard, S., Wuilleme, S., Vedrenne, A., Guiheneuf, E., Toussaint-Hacquard, M., and Everaere, F. (2020). Platelet counting: Ugly traps and good advice. Proposals from the French-speaking cellular hematology group (GFHC). J. Clin. Med., 9.","DOI":"10.3390\/jcm9030808"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1515\/labmed-2023-0032","article-title":"EDTA-associated pseudothrombopenia: Definition and real-world occurrence","volume":"47","author":"Markewitz","year":"2023","journal-title":"J. Lab. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1515\/labmed-2020-0037","article-title":"Influence of different methods and anticoagulants on platelet parameter measurement","volume":"44","year":"2020","journal-title":"J. Lab. Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"806","DOI":"10.1093\/ajcp\/aqw066","article-title":"Magnesium sulfate as an alternative in vitro anticoagulant for the measurement of platelet parameters?","volume":"145","author":"Kohlschein","year":"2016","journal-title":"Am. J. Clin. Pathol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1046\/j.1365-2141.2002.03967.x","article-title":"Mechanisms involved in the antiplatelet activity of magnesium in human platelets","volume":"119","author":"Sheu","year":"2002","journal-title":"Br. J. Haematol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1111\/ijlh.13966","article-title":"Comparison of platelet count results on the Sysmex XN between citrate or MgSO4 and K2 EDTA anticoagulants?","volume":"45","author":"Soulard","year":"2023","journal-title":"Int. J. Lab. Hematol."},{"key":"ref_8","unstructured":"European Federation of Clinical Chemistry and Laboratory Medicine (2024, April 01). EFLM Biological Variation Database [Website]. Available online: https:\/\/biologicalvariation.eu."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1111\/1467-9639.00136","article-title":"Regression through the origin","volume":"25","author":"Eisenhauer","year":"2003","journal-title":"Teach. Stat."},{"key":"ref_10","first-page":"e35","article-title":"Platelet count in sodium citrate-anticoagulated whole blood Comparison to EDTA-anticoagulated results and stability over time","volume":"3","author":"Weber","year":"2021","journal-title":"Int. J. Lab. Hematol."}],"container-title":["Journal of Clinical Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2077-0383\/13\/18\/5350\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:52:48Z","timestamp":1760111568000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2077-0383\/13\/18\/5350"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,10]]},"references-count":10,"journal-issue":{"issue":"18","published-online":{"date-parts":[[2024,9]]}},"alternative-id":["jcm13185350"],"URL":"https:\/\/doi.org\/10.3390\/jcm13185350","relation":{},"ISSN":["2077-0383"],"issn-type":[{"value":"2077-0383","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,10]]}}}