{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T22:00:09Z","timestamp":1779400809293,"version":"3.53.1"},"reference-count":52,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2024,10,18]],"date-time":"2024-10-18T00:00:00Z","timestamp":1729209600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"R01 CA256973","award":["T32CA121938"],"award-info":[{"award-number":["T32CA121938"]}]},{"name":"R01 CA259080","award":["T32CA121938"],"award-info":[{"award-number":["T32CA121938"]}]},{"name":"VA Merit Review I01 BX003856","award":["T32CA121938"],"award-info":[{"award-number":["T32CA121938"]}]},{"name":"National Institute of Health","award":["T32CA121938"],"award-info":[{"award-number":["T32CA121938"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Background\/Objectives: Pancreatic cancer is the third leading cause of death related to cancer. The only possible cure presently is complete surgical resection; however, this is limited by difficulty in clearly defining tumor margins. Enhancement of the visualization of pancreatic ductal adenocarcinoma (PDAC) tumor margins using near-infrared dye-conjugated tumor-specific antibodies was pioneered by using anti-CEA, anti-CA19.9, and anti-MUC5AC in orthotopic mouse models of pancreatic cancer. Recently, an antibody to Mucin 4 (MUC4) conjugated to a fluorescent probe has shown promise in targeting colon tumors in orthotopic mouse models. Methods: In the present study, we targeted pancreatic cancer using an anti-MUC4 antibody conjugated to IRDye800 (anti-MUC4-IR800) in orthotopic mouse models. Two pancreatic cancer human cell lines were used, SW1990 and CD18\/HPAF. Results: Anti-MUC4-IR800 targeted the two pancreatic cancer cell line tumors in orthotopic mouse models with high tumor-to-pancreas ratios and high tumor-to-liver ratios, with greater targeting seen in SW1990. Conclusions: The present results suggest anti-MUC4-IR800\u2019s potential to be used in fluorescence-guided surgical resection of pancreatic cancer.<\/jats:p>","DOI":"10.3390\/jcm13206211","type":"journal-article","created":{"date-parts":[[2024,10,18]],"date-time":"2024-10-18T06:46:52Z","timestamp":1729234012000},"page":"6211","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Targeting Human Pancreatic Cancer with a Fluorophore-Conjugated Mucin 4 (MUC4) Antibody: Initial Characterization in Orthotopic Cell Line Mouse Models"],"prefix":"10.3390","volume":"13","author":[{"given":"Sunidhi","family":"Jaiswal","sequence":"first","affiliation":[{"name":"Department of Surgery, University of California San Diego, La Jolla, CA 92093, USA"},{"name":"VA San Diego Healthcare System, La Jolla, CA 92161, 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USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-9177-2352","authenticated-orcid":false,"given":"Keita","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Surgery, University of California San Diego, La Jolla, CA 92093, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1820-088X","authenticated-orcid":false,"given":"Thinzar M.","family":"Lwin","sequence":"additional","affiliation":[{"name":"Department of Surgical Oncology, City of Hope, Duarte, CA 91010, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7141-740X","authenticated-orcid":false,"given":"Sumbal","family":"Talib","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE 68198-5870, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9035-7529","authenticated-orcid":false,"given":"Abhijit","family":"Aithal","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE 68198-5870, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kavita","family":"Mallya","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE 68198-5870, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2020-3687","authenticated-orcid":false,"given":"Maneesh","family":"Jain","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE 68198-5870, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9353-5404","authenticated-orcid":false,"given":"Aaron M.","family":"Mohs","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE 68198-5870, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert M.","family":"Hoffman","sequence":"additional","affiliation":[{"name":"Department of Surgery, University of California San Diego, La Jolla, CA 92093, USA"},{"name":"VA San Diego Healthcare System, La Jolla, CA 92161, USA"},{"name":"AntiCancer Inc., San Diego, CA 92111, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9470-9317","authenticated-orcid":false,"given":"Surinder K.","family":"Batra","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE 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