{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T17:31:31Z","timestamp":1787592691397,"version":"build-2736575974"},"reference-count":33,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2024,11,25]],"date-time":"2024-11-25T00:00:00Z","timestamp":1732492800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Background: Endometrial cancer is currently the sixth most frequent cancer in women, and scientific research is focusing on the search for particular features of the endometrium that may explain a further predisposition to the onset of endometrial cancer, aimed at improving knowledge of the pathogenetic factors of this disease. The aim of our review is to analyze in detail the results of the literature on the endometrial microbiota in patients with endometrial cancer and to investigate its role. Methods: We performed our research on the Pubmed, Web of Science, and Scopus databases. We searched up to December 2023 and considered manuscripts published from 2000. Only articles in English were included in the search. We excluded studies in which the endometrial microbiota were collected through the vagina or cervical canal. Results: We included in our review a total of five manuscripts at the end of the screening process, and the total number of patients involved was 190. Four studies considered only post-menopausal patients, while one study considered both pre- and post-menopausal patients. In all studies, the microbiota analysis was derived from a post-hysterectomy biopsy. From our review, it emerged that Bacteroidetes, Actinobacteria, Firmicutes, and Proteobacteria are the most represented bacteria in patients with endometrial cancer. These are both Gram-positive and Gram-negative, but predominantly anaerobic bacteria. Conclusions: The reduced microbial diversity and the presence of specific bacteria is often associated with endometrial cancer. Further work on larger population samples, and on healthy women and those affected by endometrial carcinoma, is needed to understand how the endometrial microbiota changes and influences the development of the tumor and whether intervening in the changes in the microbiota will have a therapeutic impact on endometrial carcinoma.<\/jats:p>","DOI":"10.3390\/jcm13237135","type":"journal-article","created":{"date-parts":[[2024,11,25]],"date-time":"2024-11-25T09:59:34Z","timestamp":1732528774000},"page":"7135","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["The Role of the Endometrial Microbiota in Endometrial Cancer: A Systematic Review of the Literature"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9266-3896","authenticated-orcid":false,"given":"Guglielmo","family":"Stabile","sequence":"first","affiliation":[{"name":"Department of Medical and Surgical Sciences, Institute of Obstetrics and Gynecology, University of Foggia, 71121 Foggia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Doria","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences, Institute of Obstetrics and Gynecology, University of Foggia, 71121 Foggia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4372-4220","authenticated-orcid":false,"given":"Matteo","family":"Bruno","sequence":"additional","affiliation":[{"name":"UOC Ginecologia Oncologica, Dipartimento per le Scienze della Salute della Donna e del Bambino e di Sanit\u00e0 Pubblica, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6588-8686","authenticated-orcid":false,"given":"Marco","family":"D\u2019Indinosante","sequence":"additional","affiliation":[{"name":"UOC Ginecologia Oncologica, Dipartimento per le Scienze della Salute della Donna e del Bambino e di Sanit\u00e0 Pubblica, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valerio","family":"Gallotta","sequence":"additional","affiliation":[{"name":"UOC Ginecologia Oncologica, Dipartimento per le Scienze della Salute della Donna e del Bambino e di Sanit\u00e0 Pubblica, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Fanfani","sequence":"additional","affiliation":[{"name":"UOC Ginecologia Oncologica, Dipartimento per le Scienze della Salute della Donna e del Bambino e di Sanit\u00e0 Pubblica, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Rome, Italy"},{"name":"Universit\u00e0 Cattolica del Sacro Cuore, 00168 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Scambia","sequence":"additional","affiliation":[{"name":"UOC Ginecologia Oncologica, Dipartimento per le Scienze della Salute della Donna e del Bambino e di Sanit\u00e0 Pubblica, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Rome, Italy"},{"name":"Universit\u00e0 Cattolica del Sacro Cuore, 00168 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7848-0329","authenticated-orcid":false,"given":"Stefano","family":"Restaino","sequence":"additional","affiliation":[{"name":"Department of Medicinal Area (DAME) Clinic of Obstetrics and Gynecology, Santa Maria della Misericordia Hospital, Azienda Sanitaria Universitaria Friuli Centrale, 33100 Udine, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2424-2691","authenticated-orcid":false,"given":"Giuseppe","family":"Vizzielli","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology Clinic, Department of Medicine, University of Udine, 33100 Udine, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2343-1677","authenticated-orcid":false,"given":"Stefania","family":"Carlucci","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences, Institute of Obstetrics and Gynecology, University of Foggia, 71121 Foggia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luigi","family":"Nappi","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences, Institute of Obstetrics and Gynecology, University of Foggia, 71121 Foggia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"209","DOI":"10.3322\/caac.21660","article-title":"Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries","volume":"71","author":"Sung","year":"2021","journal-title":"CA Cancer J. Clin."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1412","DOI":"10.1016\/S0140-6736(22)00323-3","article-title":"Endometrial cancer","volume":"399","author":"Crosbie","year":"2022","journal-title":"Lancet"},{"key":"ref_3","unstructured":"(2024, January 14). Guidelines Aiom Neoplasms of the Uterus: Endometrium and Cervix Ed. 2022 Updated to September 2022. Available online: https:\/\/www.aiom.it\/linee-guida-aiom-2022-neoplasie-dellutero-endometrio-e-cervice\/."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1719","DOI":"10.1002\/ijc.31961","article-title":"Risk factors for endometrial cancer: An umbrella review of the literature","volume":"145","author":"Raglan","year":"2019","journal-title":"Int. J. Cancer"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Esposito, G., Bravi, F., Serraino, D., Parazzini, F., Crispo, A., Augustin, L.S.A., Negri, E., La Vecchia, C., and Turati, F. (2021). Diabetes Risk Reduction Diet and Endometrial Cancer Risk. Nutrients, 13.","DOI":"10.3390\/nu13082630"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e2332296","DOI":"10.1001\/jamanetworkopen.2023.32296","article-title":"Reproductive Factors and Endometrial Cancer Risk Among Women","volume":"6","author":"Katagiri","year":"2023","journal-title":"JAMA Netw. Open"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"317","DOI":"10.3349\/ymj.2020.61.4.317","article-title":"Risk Factors Associated with Endometrial Pathology in Premenopausal Breast Cancer Patients Treated with Tamoxifen","volume":"61","author":"Lee","year":"2020","journal-title":"Yonsei Med. J."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Mora\u0144ska, K., Englert-Golon, M., Durda-Masny, M., Sajdak, S., Grabowska, M., and Szwed, A. (2023). Why Does Your Uterus Become Malignant? The Impact of the Microbiome on Endometrial Carcinogenesis. Life, 13.","DOI":"10.3390\/life13122269"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1016\/j.ajog.2016.09.085","article-title":"Challenging dogma: The endometrium has a microbiome with functional consequences!","volume":"215","author":"Giudice","year":"2016","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1097\/LGT.0000000000000646","article-title":"The Vaginal Microbiome: IV. The Role of Vaginal Microbiome in Reproduction and in Gynecologic Cancers","volume":"26","author":"Ventolini","year":"2022","journal-title":"J. Low. Genit. Tract. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Barczy\u0144ski, B., Fr\u0105szczak, K., Grywalska, E., Kotarski, J., and Korona-G\u0142owniak, I. (2023). Vaginal and Cervical Microbiota Composition in Patients with Endometrial Cancer. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24098266"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Istrate-Ofi\u0163eru, A.M., Mogoant\u0103, C.A., Zoril\u0103, G.L., Ro\u015fu, G.C., Dr\u0103gu\u015fin, R.C., Berbecaru, E.I., Zoril\u0103, M.V., Com\u0103nescu, C.M., Mogoant\u0103, S.\u0218., and Vaduva, C.C. (2024). Clinical Characteristics and Local Histopathological Modulators of Endometriosis and Its Progression. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25031789"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"n71","DOI":"10.1136\/bmj.n71","article-title":"The PRISMA 2020 statement: An updated guideline for reporting systematic reviews","volume":"372","author":"Page","year":"2021","journal-title":"BMJ"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1186\/s13073-016-0368-y","article-title":"Potential Contribution of the Uterine Microbiome in the Development of Endometrial Cancer","volume":"8","author":"Chen","year":"2016","journal-title":"Genome Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1708","DOI":"10.1002\/ijc.33428","article-title":"Dysbiosis of the endometrial microbiota and its association with inflammatory cytokines in endometrial cancer","volume":"148","author":"Lu","year":"2021","journal-title":"Int. J. Cancer"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Gressel, G.M., Usyk, M., Frimer, M., Kuo, D.Y.S., and Burk, R.D. (2021). Characterization of the endometrial, cervicovaginal and anorectal microbiota in post-menopausal women with endometrioid and serous endometrial cancers. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0259188"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/j.ygyno.2022.02.021","article-title":"Differences in the microbial profiles of early stage endometrial cancers between Black and White women","volume":"165","author":"Hawkins","year":"2022","journal-title":"Gynecol. Oncol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1168","DOI":"10.1097\/GME.0000000000002053","article-title":"Endometrial microbiota from endometrial cancer and paired pericancer tissues in postmenopausal women: Differences and clinical relevance","volume":"29","author":"Wang","year":"2022","journal-title":"Menopause"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1016\/j.ajog.2018.02.012","article-title":"The diagnosis of chronic endometritis in infertile asymptomatic women: A comparative study of histology, microbial cultures, hysteroscopy, and molecular microbiology","volume":"218","author":"Moreno","year":"2018","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1093\/humupd\/dmy012","article-title":"How uterine microbiota might be responsible for a receptive, fertile endometrium","volume":"24","author":"Benner","year":"2018","journal-title":"Hum. Reprod. Update"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Peric, A., Weiss, J., Vulliemoz, N., Baud, D., and Stojanov, M. (2019). Bacterial Colonization of the Female Upper Genital Tract. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20143405"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Fiorentini, C., Carlini, F., Germinario, E.A.P., Maroccia, Z., Travaglione, S., and Fabbri, A. (2020). Gut Microbiota and Colon Cancer: A Role for Bacterial Protein Toxins?. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21176201"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1158\/2767-9764.CRC-22-0075","article-title":"The vaginal microbiome is associated with endometrial cancer grade and histology","volume":"2","author":"Hakimjavadi","year":"2022","journal-title":"Cancer Res. Commun."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Sobstyl, M., Brecht, P., Sobstyl, A., Mertowska, P., and Grywalska, E. (2022). The Role of Microbiota in the Immunopathogenesis of Endometrial Cancer. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23105756"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Walsh, D.M., Hokenstad, A.N., Chen, J., Sung, J., Jenkins, G.D., Chia, N., Nelson, H., Mariani, A., and Walther-Antonio, M.R.S. (2019). Postmenopause as a Key Factor in the Composition of the Endometrial Cancer Microbiome (ECbiome). Sci. Rep., 9.","DOI":"10.1038\/s41598-019-55720-8"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Li, C., Gu, Y., He, Q., Huang, J., Song, Y., Wan, X., and Li, Y. (2021). Integrated Analysis of Microbiome and Transcriptome Data Reveals the Interplay Between Commensal Bacteria and Fibrin Degradation in Endometrial Cancer. Front. Cell Infect. Microbiol., 11.","DOI":"10.3389\/fcimb.2021.748558"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1080\/19490976.2017.1379637","article-title":"Mucosa-associated microbiota dysbiosis in colitis associated cancer","volume":"9","author":"Richard","year":"2018","journal-title":"Gut Microbes."},{"key":"ref_28","first-page":"423","article-title":"Pelvic inflammatory disease (PID) from Chlamydia trachomatis versus PID from Neisseria gonorrhea: From clinical suspicion to therapy","volume":"147","author":"Banco","year":"2012","journal-title":"G. Ital. Dermatol. Venereol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1002\/rmb2.12250","article-title":"A pilot study and case reports on endometrial microbiota and pregnancy outcome: An analysis using 16S rRNA gene sequencing among IVF patients, and trial therapeutic intervention for dysbiotic endometrium","volume":"18","author":"Kyono","year":"2019","journal-title":"Reprod. Med. Biol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1989","DOI":"10.1093\/infdis\/jiu004","article-title":"Bacteria in the vaginal microbiome alter the innate immune response and barrier properties of the human vaginal epithelia in a species-specific manner","volume":"209","author":"Doerflinger","year":"2014","journal-title":"J. Infect. Dis."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Chen, P., Guo, Y., Jia, L., Wan, J., He, T., Fang, C., and Li, T. (2021). Interaction between functionally activateendometrial microbiota and host gene regulation in endometrial cancer. Front. Cell Dev. Biol., 9.","DOI":"10.3389\/fcell.2021.727286"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1080\/13697137.2023.2223923","article-title":"The role of microbiota in the management of genitourinary syndrome of menopause","volume":"26","author":"Stabile","year":"2023","journal-title":"Climacteric"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Stabile, G., Gentile, R.M., Carlucci, S., Restaino, S., and De Seta, F. (2021). A New Therapy for Uncomplicated Vulvovaginal Candidiasis and Its Impact on Vaginal Flora. Healthcare, 9.","DOI":"10.3390\/healthcare9111555"}],"container-title":["Journal of Clinical Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2077-0383\/13\/23\/7135\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:39:10Z","timestamp":1760114350000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2077-0383\/13\/23\/7135"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,25]]},"references-count":33,"journal-issue":{"issue":"23","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["jcm13237135"],"URL":"https:\/\/doi.org\/10.3390\/jcm13237135","relation":{},"ISSN":["2077-0383"],"issn-type":[{"value":"2077-0383","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,25]]}}}