{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T12:01:10Z","timestamp":1774958470029,"version":"3.50.1"},"reference-count":30,"publisher":"MDPI AG","issue":"23","license":[{"start":{"date-parts":[[2024,11,30]],"date-time":"2024-11-30T00:00:00Z","timestamp":1732924800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Greek Research Infrastructure for Personalised Medicine (pMedGR)","award":["MIS 5002802"],"award-info":[{"award-number":["MIS 5002802"]}]},{"name":"The Greek Research Infrastructure for Personalised Medicine (pMedGR)","award":["NSRF 2014\u20132020"],"award-info":[{"award-number":["NSRF 2014\u20132020"]}]},{"name":"Competitiveness, Entrepreneurship and In-novation","award":["MIS 5002802"],"award-info":[{"award-number":["MIS 5002802"]}]},{"name":"Competitiveness, Entrepreneurship and In-novation","award":["NSRF 2014\u20132020"],"award-info":[{"award-number":["NSRF 2014\u20132020"]}]},{"name":"Greece and the European Union (European Re-gional Development Fund)","award":["MIS 5002802"],"award-info":[{"award-number":["MIS 5002802"]}]},{"name":"Greece and the European Union (European Re-gional Development Fund)","award":["NSRF 2014\u20132020"],"award-info":[{"award-number":["NSRF 2014\u20132020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Background: Alterations in glycolipid and glycosphingolipid pathways lead to compromised cell membranes and may be involved in cataract formation. However, the exact role of glycolipids in lens opacification is not completely understood. The aim of the current study is to investigate proteome complexity and the role of glycolipid and glycosphingolipid pathways in cataract formation. Methods: The anterior capsule and phacoemulsification (phaco) cassette contents were collected during cataract surgery from eleven participants with diabetic cataract (DC), twelve participants with age-related cataract (ARC), and seven participants with post-vitrectomy cataract (PVC). Liquid chromatography\u2013mass spectrometry with data-independent acquisition (DIA) was used for the identification and quantification of proteins. Results: The results of this study revealed that the main significantly differentially expressed pathways in the ARC group compared to the DC and PVC groups in phaco cassette samples included the glycolipid metabolic, glycosphingolipid biosynthetic, and glycosphingolipid metabolic processes, with GLA being among the most significant proteins in the ARC group. Similarly, in the anterior capsule samples, the main significantly differentially expressed pathways in the ARC group compared to the DC and PVC groups were the glycolipid metabolic, glycosphingolipid biosynthetic, and glycosphingolipid metabolic processes, with ST3GAL5 being among the most significant proteins in the ARC group. Conclusion: Glycolipid and glycosphingolipid metabolic processes may be involved in cataract formation. ST3GAL5 may modify the cell-to-cell interaction induced by cell surface sugar chains, leading to the formation and progression of cataract. GLA, associated with the breakdown of glycolipids, may lead to cataract formation when a certain threshold is surpassed, secondary to increased glycolipid metabolism.<\/jats:p>","DOI":"10.3390\/jcm13237287","type":"journal-article","created":{"date-parts":[[2024,12,3]],"date-time":"2024-12-03T11:48:56Z","timestamp":1733226536000},"page":"7287","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Proteomic Changes of Glycolipid Pathways in Age-Related, Diabetic, and Post-Vitrectomy Cataracts"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9349-4043","authenticated-orcid":false,"given":"Christina","family":"Karakosta","sequence":"first","affiliation":[{"name":"School of Medicine, National and Kapodistrian University of Athens, 11527 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9952-0636","authenticated-orcid":false,"given":"Martina","family":"Samiotaki","sequence":"additional","affiliation":[{"name":"Biomedical Sciences Research Center \u201cAlexander Fleming\u201d, 16672 Vari, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Panayotou","sequence":"additional","affiliation":[{"name":"Biomedical Sciences Research Center \u201cAlexander Fleming\u201d, 16672 Vari, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dimitrios","family":"Papaconstantinou","sequence":"additional","affiliation":[{"name":"1st University Eye Clinic, \u201cG. Gennimatas\u201d General Hospital of Athens, National and Kapodistrian University of Athens, 11527 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilita M.","family":"Moschos","sequence":"additional","affiliation":[{"name":"Department of Electrophysiology of Vision, 1st University Eye Clinic of Athens, 11527 Athens, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1146\/annurev.pu.17.050196.001111","article-title":"Blindness Due to Cataract: Epidemiology and Prevention","volume":"17","author":"Javitt","year":"1996","journal-title":"Annu. Rev. 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