{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T15:38:32Z","timestamp":1791646712465,"version":"4.3.4"},"reference-count":39,"publisher":"MDPI AG","issue":"24","license":[{"start":{"date-parts":[[2024,12,13]],"date-time":"2024-12-13T00:00:00Z","timestamp":1734048000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Objective: To evaluate the clinical outcomes of cornea transplantation (penetrating keratoplasty, Descemet membrane endothelial keratoplasty, Descemet stripping automated endothelial keratoplasty, and deep anterior lamellar keratoplasty) using donor corneas stored in Eusol-C hypothermic storage medium compared to corneas stored in organ-culture. Methods: The clinical outcomes of 92 patients who underwent corneal transplantation with human donor corneas stored in Eusol-C medium at 2\u20138 \u00b0C were retrospectively evaluated. The control group consisted of 169 patients who received corneas organ-cultured at 31 \u00b0C. Donor age, sex, death-to-preservation time, and storage time were recorded. Endothelial cell (EC) density (ECD), EC mortality, and EC morphology scores were evaluated during storage in both groups. Complication rates, visual outcomes, and corneal transparency were monitored for up to six months. Results: The mean storage in Eusol-C time was 7.7 \u00b1 2.5 days, while organ-culture time was 14.2 \u00b1 4.0 days. In the Eusol-C group, ECD was 2398 \u00b1 354 cells\/mm2, with an average EC morphology score of 3.4 \u00b1 0.7\/4. Approximately 28% of the corneas in the Eusol-C group had no EC mortality. In the organ-culture group, ECD was 2256 \u00b1 328 cells\/mm2, with an average EC morphology score of 3.5 \u00b1 0.5\/4, and 42% were devoid of EC mortality. No complications, such as re-bubbling, were observed in both groups during surgery. Transparent corneas were achieved in 81.3% of the Eusol-C group the day after surgery. Mean corrected distance visual acuity (CDVA) at 3 and 6 months was 4.5 \u00b1 4.0\/10 and 5.4 \u00b1 3.7\/10 for the Eusol-C group and 5.0 \u00b1 2.9\/10 and 5.7 \u00b1 2.8\/10 for the organ-culture group, with no statistical differences observed between the groups. No graft failure was observed up to three months. Graft rejection occurred in the Eusol-C group and in the organ-culture group in, respectively, one and two cases at the six-month follow-up. Conclusions: Comparable surgical outcomes were achieved with donor corneas stored in both hypothermic Eusol-C and organ-culture media.<\/jats:p>","DOI":"10.3390\/jcm13247606","type":"journal-article","created":{"date-parts":[[2024,12,13]],"date-time":"2024-12-13T08:10:47Z","timestamp":1734077447000},"page":"7606","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Retrospective Clinical Outcomes of Keratoplasty Using Human Donor Corneas Preserved in Eusol-C Hypothermic Storage Medium"],"prefix":"10.3390","volume":"13","author":[{"given":"Rossella Anna Maria","family":"Colabelli Gisoldi","sequence":"first","affiliation":[{"name":"Ophthalmology Unit\u2013Eye Bank of Rome, San Giovanni Addolorata Hospital, 00184 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulio","family":"Pocobelli","sequence":"additional","affiliation":[{"name":"Ophthalmology Unit, Department of Experimental Medicine, University of Rome Tor Vergata, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4910-1617","authenticated-orcid":false,"given":"Umberto","family":"Rodella","sequence":"additional","affiliation":[{"name":"Research and Development, AL.CHI.MI.A. S.R.L., 35020 Ponte San Nicol\u00f2, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1949-5213","authenticated-orcid":false,"given":"Laura","family":"Giurgola","sequence":"additional","affiliation":[{"name":"Research and Development, AL.CHI.MI.A. S.R.L., 35020 Ponte San Nicol\u00f2, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudio","family":"Gatto","sequence":"additional","affiliation":[{"name":"Research and Development, AL.CHI.MI.A. S.R.L., 35020 Ponte San Nicol\u00f2, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gemma","family":"Lodato","sequence":"additional","affiliation":[{"name":"Ophthalmology Unit\u2013Eye Bank of Rome, San Giovanni Addolorata Hospital, 00184 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giacinta","family":"Buffon","sequence":"additional","affiliation":[{"name":"Ophthalmology Unit, Department of Experimental Medicine, University of Rome Tor Vergata, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlo","family":"Nucci","sequence":"additional","affiliation":[{"name":"Ophthalmology Unit, Department of Experimental Medicine, University of Rome Tor Vergata, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1498-615X","authenticated-orcid":false,"given":"Jana","family":"D\u2019Amato T\u00f3thov\u00e1","sequence":"additional","affiliation":[{"name":"Research and Development, AL.CHI.MI.A. S.R.L., 35020 Ponte San Nicol\u00f2, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Augusto","family":"Pocobelli","sequence":"additional","affiliation":[{"name":"Ophthalmology Unit\u2013Eye Bank of Rome, San Giovanni Addolorata Hospital, 00184 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1749","DOI":"10.1016\/S0140-6736(12)60437-1","article-title":"Corneal transplantation","volume":"379","author":"Tan","year":"2012","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1001\/jamaophthalmol.2015.4776","article-title":"Global survey of corneal transplantation and eye banking","volume":"134","author":"Gain","year":"2016","journal-title":"JAMA Ophthalmol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/17469899.2021.1829476","article-title":"Corneal storage methods: Considerations and impact on surgical outcomes","volume":"16","author":"Wojcik","year":"2021","journal-title":"Expert Rev. 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