{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T00:48:44Z","timestamp":1774399724781,"version":"3.50.1"},"reference-count":38,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2024,12,28]],"date-time":"2024-12-28T00:00:00Z","timestamp":1735344000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Research Foundation of Korea\u2019s First-in-Class Research Programme","award":["20230245"],"award-info":[{"award-number":["20230245"]}]},{"name":"Soonchunhyang University","award":["20230245"],"award-info":[{"award-number":["20230245"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Background\/Objectives: This study explores the potential of using elevated levels of blood double-stranded RNA (dsRNA) as a diagnostic tool for severe fever with thrombocytopenia syndrome (SFTS) infection. Methods: Blood samples from SFTS patients were collected, dsRNA was purified, and total dsRNA expression was quantitatively analyzed using a spiropyran-based method. Comparative analysis was performed using blood samples from healthy individuals and scrub typhus patients with similar symptoms. Results: The results revealed that individuals infected with SFTS had significantly higher total blood dsRNA levels compared to healthy or scrub typhus controls. The dsRNA-based method also has potential for assessing infection severity based on dsRNA levels. Conclusions: These findings suggest that total dsRNA expression can serve as a quick and convenient method to differentiate SFTS from other non-viral conditions with similar clinical presentations. This method shows promise as a novel diagnostic tool.<\/jats:p>","DOI":"10.3390\/jcm14010105","type":"journal-article","created":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T04:10:23Z","timestamp":1735618223000},"page":"105","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Double-Stranded RNA-Based Method for Diagnosing Severe Fever with Thrombocytopenia"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9603-1404","authenticated-orcid":false,"given":"Jung Wan","family":"Park","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Division of Infectious Disease, Soonchunhyang University Hospital, Cheonan 31151, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0666-9667","authenticated-orcid":false,"given":"Jaemin","family":"Jeon","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3064-4643","authenticated-orcid":false,"given":"Yoosik","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min Hyok","family":"Jeon","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Infectious Disease, Soonchunhyang University Hospital, Cheonan 31151, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1892","DOI":"10.3201\/eid1911.130792","article-title":"Severe fever with thrombocytopenia syndrome, South Korea, 2012","volume":"19","author":"Kim","year":"2013","journal-title":"Emerg. 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