{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:50:07Z","timestamp":1760057407239,"version":"build-2065373602"},"reference-count":50,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,1,31]],"date-time":"2025-01-31T00:00:00Z","timestamp":1738281600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"D\u00fczce University Scientific Research Projects coordination unit","award":["2022.04.03\u20141309"],"award-info":[{"award-number":["2022.04.03\u20141309"]}]},{"name":"D\u00dcBAP","award":["2022.04.03\u20141309"],"award-info":[{"award-number":["2022.04.03\u20141309"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Background: The objective of this study was to evaluate serum soluble lectin-like oxidized low-density lipoprotein receptor-1 (sLOX-1) levels in children with type-1 diabetes mellitus (T1DM) without any atherosclerotic complications and to investigate whether there was an association with early atherosclerotic processes in these children. Methods: The study\u2019s design entailed a prospective cross-sectional observational study methodology. The patient group consisted of 80 consecutive children aged 8\u201318 years who had been diagnosed with T1DM for at least ten years and had not developed any chronic clinical complications related to T1DM. The control group consisted of 72 completely healthy children with similar demographic characteristics. Serum levels of sLOX-1 were measured, and carotid intima-media thickness (cIMT) was evaluated using ultrasonography in all subjects. Results: A statistical analysis of the results was conducted. The serum sLOX-1 level was found to be significantly higher in the patient group than in the control group (0.49 \u00b1 0.11 vs. 0.82 \u00b1 0.35; p &lt; 0.001). The statistical significance observed was maintained in the multivariable logistic regression analysis (p &lt; 0.001). A significant correlation was identified between cIMT and serum sLOX-1 levels (r = 0.669, p &lt; 0.001). The receiver operating characteristic curve for sLOX-1 indicated that a cutoff value greater than 0.65 ng\/mL was associated with T1DM. Conclusions: Serum sLOX-1 levels were markedly elevated in children with T1DM who had not yet manifested chronic complications. These findings suggest that elevated serum sLOX-1 levels may be associated with the progression of atherosclerosis in children with T1DM.<\/jats:p>","DOI":"10.3390\/jcm14030935","type":"journal-article","created":{"date-parts":[[2025,1,31]],"date-time":"2025-01-31T10:14:30Z","timestamp":1738318470000},"page":"935","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of Serum Soluble Lectin-like Oxidised Low-Density Lipoprotein Receptor-1 (sLOX-1) Level in Children with Non-Complicated Type-1 Diabetes Mellitus (T1DM) and Its Relationship with Carotid Intima Media Thickness (cIMT)"],"prefix":"10.3390","volume":"14","author":[{"given":"Sukriye","family":"Ozde","sequence":"first","affiliation":[{"name":"Department of General Pediatric, Faculty of Medicine, Duzce University, 81620 Duzce, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatma","family":"Yavuzyilmaz","sequence":"additional","affiliation":[{"name":"Department of Pediatric Endocrinology, Faculty of Medicine, Duzce University, 81620 Duzce, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehmet Ali","family":"Ozel","sequence":"additional","affiliation":[{"name":"Department of Radiology, Faculty of Medicine, Duzce University, 81620 Duzce, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6607-895X","authenticated-orcid":false,"given":"Osman","family":"Kayapinar","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Faculty of Medicine, Duzce University, 81620 Duzce, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cem","family":"Ozde","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Faculty of Medicine, Duzce University, 81620 Duzce, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gulsah","family":"Akture","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Faculty of Medicine, Duzce University, 81620 Duzce, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4975-9718","authenticated-orcid":false,"given":"Ilknur","family":"Arslanoglu","sequence":"additional","affiliation":[{"name":"Department of Pediatric Endocrinology, Faculty of Medicine, Duzce University, 81620 Duzce, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1360","DOI":"10.2337\/dc08-0107","article-title":"Relationship between risk factors and mortality in type 1 diabetic patients in Europe: The EURODIAB Prospective Complications Study (PCS)","volume":"31","author":"Chaturvedi","year":"2008","journal-title":"Diabetes Care"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1972","DOI":"10.1056\/NEJMoa1408214","article-title":"Glycemic control and excess mortality in type 1 diabetes","volume":"371","author":"Lind","year":"2014","journal-title":"N. Engl. J. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1016\/S0140-6736(18)31506-X","article-title":"Excess mortality and cardiovascular disease in young adults with type 1 diabetes in relation to age at onset: A nationwide, register-based cohort study","volume":"392","author":"Rawshani","year":"2019","journal-title":"Lancet"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Torkar, A.D., Plesnik, E., Groselj, U., Battelino, T., and Kotnik, P. (2020). Carotid intima-media thickness in healthy children and adolescents: Normative data and systematic literature review. Front. Cardiovasc. Med., 7.","DOI":"10.3389\/fcvm.2020.597768"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1007\/s12170-012-0274-9","article-title":"Cardiovascular risk in children and adolescents with type 1 and type 2 diabetes mellitus","volume":"6","author":"Magge","year":"2012","journal-title":"Curr. Cardiovasc. Risk Rep."},{"key":"ref_6","first-page":"e603","article-title":"Cardiovascular risk reduction in high-risk pediatric patients: A scientific statement from the American Heart Association","volume":"139","author":"Steinberger","year":"2019","journal-title":"Circulation"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1161\/CIR.0000000000000034","article-title":"Type 1 diabetes mellitus and cardiovascular disease: A scientific statement from the American Heart Association and American Diabetes Association","volume":"130","author":"Fonseca","year":"2014","journal-title":"Circulation"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1750","DOI":"10.1161\/01.CIR.0000124725.46165.2C","article-title":"Endothelial dysfunction and increased arterial intima-media thickness in children with type 1 diabetes","volume":"109","author":"Raitakari","year":"2004","journal-title":"Circulation"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1186\/s12944-017-0579-2","article-title":"Current knowledge on the mechanism of atherosclerosis and pro-atherosclerotic properties of oxysterols","volume":"16","author":"Szterk","year":"2017","journal-title":"Lipids Health Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.ijcha.2018.09.006","article-title":"Molecular mechanisms and genetic regulation in atherosclerosis","volume":"21","author":"Jackson","year":"2018","journal-title":"IJC Heart Vasc."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e013803","DOI":"10.1161\/JAHA.119.013803","article-title":"Soluble LOX-1: A novel biomarker in patients with coronary artery disease, stroke, and acute aortic dissection?","volume":"9","author":"Hofmann","year":"2020","journal-title":"J. Am. Heart Assoc."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.cca.2019.01.006","article-title":"LOX-1 and atherosclerotic-related diseases","volume":"491","author":"Jin","year":"2019","journal-title":"Clin. Chim. Acta"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Kattoor, A.J., Goel, A., and Mehta, J.L. (2019). LOX-1: Regulation, signaling and its role in atherosclerosis. Antioxidants, 8.","DOI":"10.3390\/antiox8070218"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e009874","DOI":"10.1161\/JAHA.118.009874","article-title":"High levels of soluble lectin-like oxidized low-density lipoprotein receptor-1 are associated with carotid plaque inflammation and increased risk of ischemic stroke","volume":"8","author":"Markstad","year":"2019","journal-title":"J. Am. Heart Assoc."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1111\/pedi.12773","article-title":"ISPAD Clinical Practice Consensus Guidelines 2018: Definition, epidemiology, and classification of diabetes in children and adolescents","volume":"19","author":"Kahkoska","year":"2018","journal-title":"Pediatr. Diabetes"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1111\/pedi.12737","article-title":"ISPAD Clinical Practice Consensus Guidelines 2018: Glycemic control targets and glucose monitoring for children, adolescents, and young adults with diabetes","volume":"19","author":"DiMeglio","year":"2018","journal-title":"Pediatr. Diabetes"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1111\/pedi.12742","article-title":"ISPAD Clinical Practice Consensus Guidelines 2018: Microvascular and macrovascular complications in children and adolescents","volume":"19","author":"Donaghue","year":"2018","journal-title":"Pediatr. Diabetes"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1161\/HYPERTENSIONAHA.109.192639","article-title":"Noninvasive assessment of subclinical atherosclerosis in children and adolescents: Recommendations for standard assessment for clinical research: A scientific statement from the American Heart Association","volume":"54","author":"Urbina","year":"2009","journal-title":"Hypertension"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1210","DOI":"10.1373\/clinchem.2005.064808","article-title":"ELISA for soluble form of lectin-like oxidized LDL receptor-1, a novel marker of acute coronary syndrome","volume":"52","author":"Ueda","year":"2006","journal-title":"Clin. Chem."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2043","DOI":"10.2337\/dc10-0505","article-title":"Early signs of atherosclerosis in diabetic children on intensive insulin treatment: A population-based study","volume":"33","author":"Margeirsdottir","year":"2010","journal-title":"Diabetes Care"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1111\/pedi.12557","article-title":"Intima-media thickness at different arterial segments in pediatric type 1 diabetes patients and its relationship with advanced glycation end products","volume":"19","author":"Lilje","year":"2018","journal-title":"Pediatr. Diabetes"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1515\/jpem-2018-0382","article-title":"Cardiovascular risk factors in children with type 1 diabetes mellitus","volume":"32","author":"Zhang","year":"2019","journal-title":"J. Pediatr. Endocrinol. Metab."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1002\/jcla.21748","article-title":"Soluble lectin-like oxidized LDL receptor 1 as a possible mediator of endothelial dysfunction in patients with metabolic syndrome","volume":"29","author":"Civelek","year":"2015","journal-title":"J. Clin. Lab. Anal."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1016\/j.repbio.2020.04.006","article-title":"Polycystic ovary syndrome and endothelial dysfunction: A potential role for soluble lectin-like oxidized low-density lipoprotein receptor-1","volume":"20","author":"Oncul","year":"2020","journal-title":"Reprod. Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e8145","DOI":"10.1161\/JAHA.122.027042","article-title":"High plasma levels of soluble lectin-like oxidized low-density lipoprotein receptor-1 are associated with inflammation and cardiometabolic risk profiles in pediatric overweight and obesity","volume":"12","author":"Stinson","year":"2023","journal-title":"J. Am. Heart Assoc."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1438","DOI":"10.1194\/jlr.M700551-JLR200","article-title":"Soluble lectin-like oxidized low-density lipoprotein receptor-1 in type 2 diabetes mellitus","volume":"49","author":"Tan","year":"2008","journal-title":"J. Lipid Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1016\/j.atherosclerosis.2008.07.012","article-title":"Glycoxidized LDL increases lectin-like oxidized low-density lipoprotein receptor-1 in diabetes mellitus","volume":"203","author":"Shiu","year":"2009","journal-title":"Atherosclerosis"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1111\/1753-0407.13287","article-title":"Effect of type 2 diabetes on a disintegrin and metalloprotease 10","volume":"14","author":"Lam","year":"2022","journal-title":"J. Diabetes"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Stankova, T., Delcheva, G., Maneva, A., and Vladeva, S. (2019). Serum levels of carbamylated LDL and soluble lectin-like oxidized low-density lipoprotein receptor-1 are associated with coronary artery disease in patients with metabolic syndrome. Medicina, 55.","DOI":"10.3390\/medicina55080493"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1016\/j.metabol.2013.01.018","article-title":"LOX-1 is a novel marker for peripheral artery disease in patients with type 2 diabetes","volume":"62","author":"Fukui","year":"2013","journal-title":"Metabolism"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"20503121211064468","DOI":"10.1177\/20503121211064468","article-title":"Association of circulating soluble lectin-like oxidized low-density lipoprotein receptor-1 with inflammatory markers and urinary albumin excretion in patients with type 2 diabetes","volume":"9","author":"Takebayashi","year":"2021","journal-title":"SAGE Open Med."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"943","DOI":"10.1016\/j.echo.2006.04.020","article-title":"Clinical application of noninvasive vascular ultrasound in cardiovascular risk stratification: A report from the American Society of Echocardiography and the Society of Vascular Medicine and Biology","volume":"19","author":"Roman","year":"2006","journal-title":"J. Am. Soc. Echocardiogr."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1016\/j.jdiacomp.2015.03.018","article-title":"Increased carotid intima-media thickness (CIMT) levels in patients with type 1 diabetes mellitus (T1DM): A meta-analysis","volume":"29","author":"Sun","year":"2015","journal-title":"J. Diabetes Complicat."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2053","DOI":"10.1210\/jc.2006-2868","article-title":"Age of onset of type 1 diabetes in children and carotid intima-media thickness","volume":"92","author":"Bechtold","year":"2007","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.1016\/j.jdiacomp.2016.03.030","article-title":"Do traditional cardiovascular risk factors solely explain intima-media thickening in youth with type 1 diabetes?","volume":"30","author":"Birnie","year":"2016","journal-title":"J. Diabetes Complicat."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"e000625","DOI":"10.1136\/bmjdrc-2018-000625","article-title":"Cardiovascular risk factors from diagnosis in children with type 1 diabetes mellitus: A longitudinal cohort study","volume":"7","author":"Jones","year":"2019","journal-title":"BMJ Open Diabetes Res. Care"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0009-8981(01)00797-5","article-title":"C-reactive protein and cardiovascular disease: A review of risk prediction and interventions","volume":"317","author":"Rifai","year":"2002","journal-title":"Clin. Chim. Acta"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2348","DOI":"10.1161\/01.ATV.19.10.2348","article-title":"Complement and atherogenesis: Binding of CRP to degraded, nonoxidized LDL enhances complement activation","volume":"19","author":"Bhakdi","year":"1999","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1093\/clinchem\/47.3.426","article-title":"Absence of diurnal variation of C-reactive protein concentrations in healthy human subjects","volume":"47","author":"Ridker","year":"2001","journal-title":"Clin. Chem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1093\/clinchem\/47.3.444","article-title":"Variability and classification accuracy of serial high-sensitivity C-reactive protein measurements in healthy adults","volume":"47","author":"Ockene","year":"2001","journal-title":"Clin. Chem."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1136\/bmj.321.7255.199","article-title":"Low grade inflammation and coronary heart disease: Prospective study and updated meta-analyses","volume":"321","author":"Danesh","year":"2000","journal-title":"BMJ"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1056\/NEJM200003233421202","article-title":"C-reactive protein and other markers of inflammation in the prediction of cardiovascular disease in women","volume":"342","author":"Ridker","year":"2000","journal-title":"N. Engl. J. Med."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1007\/s001250051162","article-title":"Plasma concentration of C-reactive protein is increased in type I diabetic patients without clinical macroangiopathy and correlates with markers of endothelial dysfunction: Evidence for chronic inflammation","volume":"42","author":"Schalkwijk","year":"1999","journal-title":"Diabetologia"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.atherosclerosis.2014.11.018","article-title":"Inflammation in childhood type 1 diabetes; influence of glycemic control","volume":"238","author":"Heier","year":"2015","journal-title":"Atherosclerosis"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1758","DOI":"10.2337\/dc15-0169","article-title":"The role of markers of low-grade inflammation for the early time course of glycemic control, glucose disappearance rate, and \u03b2-cell function in recently diagnosed type 1 and type 2 diabetes","volume":"38","author":"Weber","year":"2015","journal-title":"Diabetes Care"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"e001424","DOI":"10.1136\/bmjdrc-2020-001424","article-title":"Children with type 1 diabetes have elevated high-sensitivity C-reactive protein compared with a control group","volume":"8","author":"Herrero","year":"2020","journal-title":"BMJ Open Diabetes Res. Care"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"2868","DOI":"10.1210\/jc.2009-1993","article-title":"Inflammatory markers are increased in youth with type 1 diabetes: The SEARCH case-control study","volume":"95","author":"West","year":"2010","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/0002-9343(77)90874-9","article-title":"High-density lipoprotein as a protective factor against coronary heart disease. The Framingham Study","volume":"62","author":"Gordon","year":"1977","journal-title":"Am. J. Med."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2528","DOI":"10.2337\/dc06-1161","article-title":"Type 1 diabetes and coronary artery disease","volume":"29","author":"Orchard","year":"2006","journal-title":"Diabetes Care"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3559","DOI":"10.1093\/eurheartj\/ehz114","article-title":"Elevated high-density lipoprotein in adolescents with type 1 diabetes is associated with endothelial dysfunction in the presence of systemic inflammation","volume":"40","author":"Chiesa","year":"2019","journal-title":"Eur. Heart J."}],"container-title":["Journal of Clinical Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2077-0383\/14\/3\/935\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:25:01Z","timestamp":1760027101000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2077-0383\/14\/3\/935"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,31]]},"references-count":50,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,2]]}},"alternative-id":["jcm14030935"],"URL":"https:\/\/doi.org\/10.3390\/jcm14030935","relation":{},"ISSN":["2077-0383"],"issn-type":[{"type":"electronic","value":"2077-0383"}],"subject":[],"published":{"date-parts":[[2025,1,31]]}}}