{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:59:11Z","timestamp":1760057951376,"version":"build-2065373602"},"reference-count":36,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T00:00:00Z","timestamp":1741651200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Background: Sternal wound complications following median sternotomy result in poor outcomes. Novel approaches such as placental allografts are being explored to optimize wound closure. Methods: This study evaluated consecutive patients undergoing median sternotomy by a single surgeon as sternal closure strategies evolved. Initially, wires with autologous platelet-rich plasma (PRP) were used (Group 1). Subsequently, suture tapes with PRP and an aseptically processed amnion\u2013chorion placental allograft (aACPA) were added (Group 2). Finally, PRP was discontinued (Group 3). Sternal infection, dehiscence, pain outcomes, hospital length of stay, and patient risk factors were analyzed. Results: Compared to Group 1, Groups 2 and 3 demonstrated significantly lower infection (0.7%, 0% vs. 9.3%, p = 0.0001) and dehiscence rates (0%, 0% vs. 8.7%, p &lt; 0.0001). Significant postoperative pain at two weeks decreased from Group 1 to Groups 2 and 3 (18.7%, 4.7%, 3.1%, p &lt; 0.0001), with similar improvements at one month (12.0%, 2.0%, 1.5%, p = 0.0005). Despite higher median risk factors in Group 3 than in Groups 1 and 2 (3 vs. 2, 2, p = 0.0305), a trend toward reduced hospital stay was observed (6 vs. 8, 7 days, p = 0.2298). Conclusions: Adding aACPA to sternal closure significantly reduced infections, dehiscence, and pain in high-risk cardiac surgery patients, with sustained benefits and no increase in operative times. These findings highlight aACPA\u2019s potential to mitigate sternal complications, warranting further study in larger cohorts.<\/jats:p>","DOI":"10.3390\/jcm14061877","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T06:52:30Z","timestamp":1741675950000},"page":"1877","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Implementation of Aseptically Processed Human Placental Membrane Allografts Within a Comprehensive Sternal Wound Closure Strategy: A Three-Phase Experience"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-2607-5555","authenticated-orcid":false,"given":"Zain","family":"Khalpey","sequence":"first","affiliation":[{"name":"Department of Cardiac Surgery, HonorHealth, Suite 300, 10210 N 92nd St., Scottsdale, AZ 85258, USA"},{"name":"Khalpey AI Lab, Applied & Translational AI Research Institute (ATARI), 10210 N 92nd St., Scottsdale, AZ 85258, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8655-5856","authenticated-orcid":false,"given":"Ujjawal Aditya","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, HonorHealth, Suite 300, 10210 N 92nd St., Scottsdale, AZ 85258, USA"},{"name":"Khalpey AI Lab, Applied & Translational AI Research Institute (ATARI), 10210 N 92nd St., Scottsdale, AZ 85258, USA"},{"name":"School of Clinical Medicine, University of Cambridge, Hills Road, Cambridge CB2 0SP, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3655-0261","authenticated-orcid":false,"given":"Pamela","family":"Hitscherich","sequence":"additional","affiliation":[{"name":"Department of Research & Development, MTF Biologics, Edison, NJ 08837, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zacharya","family":"Khalpey","sequence":"additional","affiliation":[{"name":"Khalpey AI Lab, Applied & Translational AI Research Institute (ATARI), 10210 N 92nd St., Scottsdale, AZ 85258, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tyler","family":"Phillips","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, HonorHealth, Suite 300, 10210 N 92nd St., Scottsdale, AZ 85258, USA"},{"name":"Khalpey AI Lab, Applied & Translational AI Research Institute (ATARI), 10210 N 92nd St., Scottsdale, AZ 85258, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evangelia","family":"Chnari","sequence":"additional","affiliation":[{"name":"Department of Research & Development, MTF Biologics, Edison, NJ 08837, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6045-8146","authenticated-orcid":false,"given":"Marc","family":"Long","sequence":"additional","affiliation":[{"name":"Department of Research & Development, MTF Biologics, Edison, NJ 08837, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Anderson, R.H., Cook, A.C., Spicer, D.E., Hlavacek, A.M., Backer, C.L., and Tretter, J.T. 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