{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T14:11:17Z","timestamp":1786457477207,"version":"build-2736575974"},"reference-count":28,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T00:00:00Z","timestamp":1743206400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Background\/Objectives: Ischemic time plays a crucial role in graft function and survival during kidney transplantation. Cooling methods, including cold perfusion and ice slush, are predominantly applied to preserve the kidney, but they may cause uneven cooling and complications. The Organ Pocket\u00ae, an insulated gel bag, has been introduced as an alternative cooling method. However, no studies have compared renal temperature changes between the Organ Pocket\u00ae and conventional cooling methods. Methods: We retrospectively analyzed 49 cases of living-donor kidney transplantation. Among these, 33 received kidney grafts preserved with the Organ Pocket\u00ae (OP group), and 16 underwent conventional cooling (control group). Renal surface temperatures were recorded at 5 min intervals during vascular anastomosis using thermography. Postoperative renal function was assessed with estimated glomerular filtration rate (eGFR), serum creatinine (sCr), and liver-type fatty acid-binding protein (L-FABP) levels. Results: The OP group demonstrated significantly higher renal surface temperatures than the control group during vascular anastomosis (p &lt; 0.05). Renal surface temperature before reperfusion was 20.4 \u00b0C \u00b1 2.5 \u00b0C and 17.2 \u00b0C \u00b1 2.5 \u00b0C in the OP and control groups, respectively. No significant differences in postoperative eGFR, sCr, and L-FABP levels; delayed graft function (DGF); or acute rejection rates were observed between the groups. Conclusions: The Organ Pocket\u00ae effectively stabilized renal temperatures during vascular anastomosis without direct cooling, thereby reducing continuous manual cooling requirements. Short-term renal function outcomes were comparable between groups; however, the Organ Pocket\u00ae may improve surgical efficiency and be particularly beneficial in robot-assisted kidney transplantation. Further studies are warranted to investigate its long-term benefits.<\/jats:p>","DOI":"10.3390\/jcm14072368","type":"journal-article","created":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T02:50:16Z","timestamp":1743389416000},"page":"2368","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comparative Analysis Between Insulated Gel Bags and Direct Cooling for Temperature Management During Kidney Transplant Vascular Anastomosis"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5758-2481","authenticated-orcid":false,"given":"Yuichi","family":"Machida","sequence":"first","affiliation":[{"name":"Department of Urology, Osaka Metropolitan University Graduate School of Medicine, 1-4-3, Asahi-Machi, Abeno-ku, Osaka 545-8585, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2021-0673","authenticated-orcid":false,"given":"Tomoaki","family":"Iwai","sequence":"additional","affiliation":[{"name":"Department of Urology, Osaka Metropolitan University Graduate School of Medicine, 1-4-3, Asahi-Machi, Abeno-ku, Osaka 545-8585, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9473-6008","authenticated-orcid":false,"given":"Kazuya","family":"Kabei","sequence":"additional","affiliation":[{"name":"Department of Urology, Osaka Metropolitan University Graduate School of Medicine, 1-4-3, Asahi-Machi, Abeno-ku, Osaka 545-8585, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junji","family":"Uchida","sequence":"additional","affiliation":[{"name":"Department of Urology, Osaka Metropolitan University Graduate School of Medicine, 1-4-3, Asahi-Machi, Abeno-ku, Osaka 545-8585, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"162","DOI":"10.1111\/tri.12892","article-title":"Kidney temperature course during living organ procurement and transplantation","volume":"30","author":"Kuipers","year":"2017","journal-title":"Transp. Int."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1016\/j.kint.2015.09.002","article-title":"Prolonged warm ischemia time is associated with graft failure and mortality after kidney transplantation","volume":"89","author":"Tennankore","year":"2016","journal-title":"Kidney Int."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1111\/j.1600-6143.2004.00441.x","article-title":"Early graft function after living donor kidney transplantation predicts rejection but not outcomes","volume":"4","author":"Brennan","year":"2004","journal-title":"Am. J. Transplant."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Urbanellis, P., Mazilescu, L., Kollmann, D., Linares-Cervantes, I., Kaths, J.M., Ganesh, S., Oquendo, F., Sharma, M., Goto, T., and Noguchi, Y. (2021). Prolonged warm ischemia time leads to severe renal dysfunction of donation-after-cardiac death kidney grafts. Sci. Rep., 11.","DOI":"10.1038\/s41598-021-97078-w"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1222","DOI":"10.1016\/j.transproceed.2012.01.118","article-title":"Deleterious influence of prolonged warm ischemia in living donor kidney transplantation","volume":"44","author":"Hellegering","year":"2012","journal-title":"Transplant. Proc."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"13","DOI":"10.6002\/ect.2018.0377","article-title":"Warm Ischemia Time at Vascular Anastomosis is an Independent Predictor for Delayed Graft Function in Kidney Transplant Recipients","volume":"18","author":"Ferede","year":"2020","journal-title":"Exp. Clin. Transplant."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2653","DOI":"10.1111\/ajt.16580","article-title":"\u201cTime is tissue\u201d-A minireview on the importance of donor nephrectomy, donor hepatectomy, and implantation times in kidney and liver transplantation","volume":"21","author":"Heylen","year":"2021","journal-title":"Am. J. Transplant."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1111\/j.1432-2277.2003.tb00305.x","article-title":"Kidney warming during transplantation","volume":"16","author":"Feuillu","year":"2003","journal-title":"Transpl. Int."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1002\/bjs.1800761004","article-title":"Reducing renal injury during transplantation","volume":"76","author":"Forsythe","year":"1989","journal-title":"Br. J. Surg."},{"key":"ref_10","first-page":"99","article-title":"Regional renal hypothermia","volume":"48","author":"Wickham","year":"1971","journal-title":"Ann. R. Coll. Surg. Engl."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3144","DOI":"10.1016\/j.transproceed.2018.09.003","article-title":"Techniques to Ameliorate the Impact of Second Warm Ischemic Time on Kidney Transplantation Outcomes","volume":"50","author":"Hameed","year":"2018","journal-title":"Transplant. Proc."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1111\/ajt.14399","article-title":"Intra-Abdominal Cooling System Limits Ischemia-Reperfusion Injury During Robot-Assisted Renal Transplantation","volume":"18","author":"Meier","year":"2018","journal-title":"Am. J. Transplant."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e928773","DOI":"10.12659\/AOT.928773","article-title":"An Effective Cooling Device for Minimal-Incision Kidney Transplantation","volume":"25","author":"Li","year":"2020","journal-title":"Ann. Transplant."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e1454","DOI":"10.1097\/TXD.0000000000001454","article-title":"Protection From Second Warm Ischemic Injury Using a Thermal Barrier Bag in Kidney Transplantation","volume":"9","author":"Ide","year":"2023","journal-title":"Transplant. Direct."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1016\/j.transproceed.2023.03.052","article-title":"Evaluating a New Device for Reducing Second Warm Ischemia During Organ Transplantation in a Porcine Model of Kidney, Heart, and Pancreas Transplantation","volume":"55","author":"Torai","year":"2023","journal-title":"Transplant. Proc."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1053\/j.ajkd.2008.12.034","article-title":"Revised equations for estimated GFR from serum creatinine in Japan","volume":"53","author":"Matsuo","year":"2009","journal-title":"Am. J. Kidney Dis."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Abou Taka, M., Dugbartey, G.J., and Sener, A. (2022). The Optimization of Renal Graft Preservation Temperature to Mitigate Cold Ischemia-Reperfusion Injury in Kidney Transplantation. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24010567"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1016\/S0041-1345(98)01892-2","article-title":"Does \u201csecond warm ischemia time\u201d play a role in kidney allograft function?","volume":"31","author":"Szostek","year":"1999","journal-title":"Transplant. Proc."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1111\/j.1464-410X.1975.tb03913.x","article-title":"Determination of the Optimum temperature for regional renal hypothermia during temporary renal ischaemia","volume":"47","author":"Ward","year":"1975","journal-title":"Br. J. Urol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1007\/s00345-023-04328-9","article-title":"Systematic review of techniques and devices used to avoid warm ischemia time injury during kidney transplantation","volume":"41","author":"Andras","year":"2023","journal-title":"World J. Urol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1177\/15533506241260087","article-title":"A Novel Cooling Device for Kidney Transplant Surgery","volume":"31","author":"Dergham","year":"2024","journal-title":"Surg. Innov."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2900","DOI":"10.1111\/ajt.13397","article-title":"The effect of anastomosis time on outcome in recipients of kidneys donated after brain death: A cohort study","volume":"15","author":"Heylen","year":"2015","journal-title":"Am. J. Transplant."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1016\/j.urolonc.2011.02.015","article-title":"Warm ischemia less than 30 minutes is not necessarily safe during partial nephrectomy: Every minute matters","volume":"29","author":"Patel","year":"2011","journal-title":"Urol. Oncol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Malyszko, J., Lukaszyk, E., Glowinska, I., and Durlik, M. (2015). Biomarkers of delayed graft function as a form of acute kidney injury in kidney transplantation. Sci. Rep., 5.","DOI":"10.1038\/srep11684"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3064","DOI":"10.1016\/j.transproceed.2011.08.038","article-title":"Liver fatty-acid-binding protein in heart and kidney allograft recipients in relation to kidney function","volume":"43","author":"Przybylowski","year":"2011","journal-title":"Transplant. Proc."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1097\/MOU.0000000000000476","article-title":"Robotic kidney transplantation: Dream or future?","volume":"28","author":"Doumerc","year":"2018","journal-title":"Curr. Opin. Urol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2651","DOI":"10.1007\/s00345-019-02934-0","article-title":"Robotic-assisted kidney transplant: A single center experience with median follow-up of 2.8 years","volume":"38","author":"Ganpule","year":"2020","journal-title":"World J. Urol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1111\/ajt.15626","article-title":"Robotic kidney transplantation in the obese patient: 10-year experience from a single center","volume":"20","author":"Tzvetanov","year":"2020","journal-title":"Am. J. Transplant."}],"container-title":["Journal of Clinical Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2077-0383\/14\/7\/2368\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:05:33Z","timestamp":1760029533000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2077-0383\/14\/7\/2368"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,29]]},"references-count":28,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2025,4]]}},"alternative-id":["jcm14072368"],"URL":"https:\/\/doi.org\/10.3390\/jcm14072368","relation":{},"ISSN":["2077-0383"],"issn-type":[{"value":"2077-0383","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,29]]}}}