{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T01:19:33Z","timestamp":1790558373851,"version":"4.1.0"},"reference-count":108,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,4,3]],"date-time":"2025-04-03T00:00:00Z","timestamp":1743638400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Background\/Objectives: Healing of colonic anastomoses is critical to surgical recovery, particularly under obstructive ileus conditions. Adhesive biological materials such as albumin\/glutaraldehyde glue (Bioglue) show potential in enhancing anastomotic healing and minimizing complications. This study investigates the effect of Bioglue on colonic anastomoses healing under obstructive ileus conditions in rats. Methods: Eighty albino Wistar rats were divided into control, ileus, Bioglue, and ileus + Bioglue groups (n = 20 each). Subgroups (n = 10) were sacrificed on the 4th or 8th postoperative day. In the control and Bioglue groups, end-to-end anastomoses were performed after colonic resection. In the ileus and ileus + Bioglue groups, obstructive ileus was induced by colonic ligation, followed by resection and primary anastomosis. Bioglue was applied in the Bioglue and ileus + Bioglue groups. Assessments included bursting pressure, peritoneal adhesion and inflammation scores, and biochemical markers (fibroblast activity, neoangiogenesis, collagen deposition, hydroxyproline, and collagenase concentrations). Results: Bursting pressure and fibroblast activity were significantly higher in the ileus + Bioglue group compared to the ileus group on both postoperative days. Although anastomotic rupture occurred in the ileus and ileus + Bioglue groups, the incidence was not significantly different from the control and Bioglue groups. Ileus + Bioglue showed significantly higher adhesion scores, inflammatory infiltration, neoangiogenesis, and collagen deposition compared to the control and ileus groups. Hydroxyproline was significantly elevated in the ileus + Bioglue group on the 8th day. Collagenase I concentrations were higher in ileus + Bioglue but not significant. Conclusions: Bioglue application enhances colonic anastomotic healing under obstructive ileus conditions, improving mechanical strength and promoting tissue repair by the 4th and 8th postoperative days. These findings support its potential clinical application.<\/jats:p>","DOI":"10.3390\/jcm14072457","type":"journal-article","created":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T06:52:56Z","timestamp":1743749576000},"page":"2457","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The Effect of Albumin\/Glutaraldehyde Glue (Bioglue) on Colonic Anastomosis Under Intestinal Obstruction: An Experimental Study in Rats"],"prefix":"10.3390","volume":"14","author":[{"given":"Kalliopi","family":"Despoudi","sequence":"first","affiliation":[{"name":"4th Department of Surgery, General Hospital \u201cGeorge Papanikolaou\u201d, Aristotle University of Thessaloniki, 57010 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioannis","family":"Mantzoros","sequence":"additional","affiliation":[{"name":"4th Department of Surgery, General Hospital \u201cGeorge Papanikolaou\u201d, Aristotle University of Thessaloniki, 57010 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0861-8783","authenticated-orcid":false,"given":"Orestis","family":"Ioannidis","sequence":"additional","affiliation":[{"name":"4th Department of Surgery, General Hospital \u201cGeorge Papanikolaou\u201d, Aristotle University of Thessaloniki, 57010 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5650-7319","authenticated-orcid":false,"given":"Elissavet","family":"Anestiadou","sequence":"additional","affiliation":[{"name":"4th Department of Surgery, General Hospital \u201cGeorge Papanikolaou\u201d, Aristotle University of Thessaloniki, 57010 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Savvas","family":"Symeonidis","sequence":"additional","affiliation":[{"name":"4th Department of Surgery, General Hospital \u201cGeorge Papanikolaou\u201d, Aristotle University of Thessaloniki, 57010 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8281-1189","authenticated-orcid":false,"given":"Stefanos","family":"Bitsianis","sequence":"additional","affiliation":[{"name":"4th Department of Surgery, General Hospital \u201cGeorge Papanikolaou\u201d, Aristotle University of Thessaloniki, 57010 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Efstathios","family":"Kotidis","sequence":"additional","affiliation":[{"name":"4th Department of Surgery, General Hospital \u201cGeorge Papanikolaou\u201d, Aristotle University of Thessaloniki, 57010 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manousos George","family":"Pramateftakis","sequence":"additional","affiliation":[{"name":"4th Department of Surgery, General Hospital \u201cGeorge Papanikolaou\u201d, Aristotle University of Thessaloniki, 57010 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonia Aikaterini","family":"Bourtzinakou","sequence":"additional","affiliation":[{"name":"4th Department of Surgery, General Hospital \u201cGeorge Papanikolaou\u201d, Aristotle University of Thessaloniki, 57010 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleni","family":"Salta-Poupnara","sequence":"additional","affiliation":[{"name":"4th Department of Surgery, General Hospital \u201cGeorge Papanikolaou\u201d, Aristotle University of Thessaloniki, 57010 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-5295-0946","authenticated-orcid":false,"given":"Konstantinos","family":"Angelopoulos","sequence":"additional","affiliation":[{"name":"4th Department of Surgery, General Hospital \u201cGeorge Papanikolaou\u201d, Aristotle University of Thessaloniki, 57010 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Driagka","sequence":"additional","affiliation":[{"name":"4th Department of Surgery, General Hospital \u201cGeorge Papanikolaou\u201d, Aristotle University of Thessaloniki, 57010 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8888-9740","authenticated-orcid":false,"given":"Freiderikos","family":"Tserkezidis","sequence":"additional","affiliation":[{"name":"4th Department of Surgery, General Hospital \u201cGeorge Papanikolaou\u201d, Aristotle University of Thessaloniki, 57010 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stamatios","family":"Angelopoulos","sequence":"additional","affiliation":[{"name":"4th Department of Surgery, General Hospital \u201cGeorge Papanikolaou\u201d, Aristotle University of Thessaloniki, 57010 Thessaloniki, Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.ijsu.2015.08.017","article-title":"The Impact of Anastomotic Leakage on Oncologic Outcomes and the Receipt and Timing of Adjuvant Chemotherapy after Colorectal Cancer Surgery","volume":"22","author":"Kim","year":"2015","journal-title":"Int. J. Surg."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1097\/DCR.0000000000001590","article-title":"Predictors for Anastomotic Leak, Postoperative Complications, and Mortality after Right Colectomy for Cancer: Results from an International Snapshot Audit","volume":"63","author":"Gallo","year":"2020","journal-title":"Dis. Colon Rectum"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Zarnescu, E.C., Zarnescu, N.O., and Costea, R. (2021). Updates of Risk Factors for Anastomotic Leakage after Colorectal Surgery. Diagnostics, 11.","DOI":"10.3390\/diagnostics11122382"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1736","DOI":"10.4240\/wjgs.v13.i12.1736","article-title":"Platelet Rich Plasma Effectiveness in Bowel Anastomoses: A Systematic Review","volume":"13","author":"Geropoulos","year":"2021","journal-title":"World J. Gastrointest. Surg."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1007\/s00384-007-0399-3","article-title":"Incidence, Consequences, and Risk Factors for Anastomotic Dehiscence after Colorectal Surgery: A Prospective Monocentric Study","volume":"23","author":"Buchs","year":"2008","journal-title":"Int. J. Color. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/j.surg.2009.10.012","article-title":"Definition and Grading of Anastomotic Leakage Following Anterior Resection of the Rectum: A Proposal by the International Study Group of Rectal Cancer","volume":"147","author":"Rahbari","year":"2010","journal-title":"Surgery"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1533","DOI":"10.1007\/s00384-015-2359-7","article-title":"The Types of Anastomotic Leakage That Develop Following Anterior Resection for Rectal Cancer Demonstrate Distinct Characteristics and Oncologic Outcomes","volume":"30","author":"Lim","year":"2015","journal-title":"Int. J. Color. Dis."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Kang, J., Kim, H., Park, H.J., Lee, B., and Lee, K.Y. (2022). Risk Factors and Economic Burden of Postoperative Anastomotic Leakage Related Events in Patients Who Underwent Surgeries for Colorectal Cancer. PLoS ONE, 17.","DOI":"10.1371\/journal.pone.0267950"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1002\/bjs.9697","article-title":"Systematic Review of Preoperative, Intraoperative and Postoperative Risk Factors for Colorectal Anastomotic Leaks","volume":"102","author":"McDermott","year":"2015","journal-title":"Br. J. Surg."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1007\/s12262-016-1556-0","article-title":"Predictive Factors for Small Intestinal and Colonic Anastomotic Leak: A Multivariate Analysis","volume":"79","author":"Sakr","year":"2017","journal-title":"Indian J. Surg."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Niu, L., Wang, J., Zhang, P., and Zhao, X. (2020). Protective Ileostomy Does Not Prevent Anastomotic Leakage after Anterior Resection of Rectal Cancer. J. Int. Med. Res., 48.","DOI":"10.1177\/0300060520946520"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1159\/000537805","article-title":"Postoperative Ileus after Minimally Invasive Colorectal Surgery: A Summary of Current Strategies for Prevention and Management","volume":"41","author":"Abernethy","year":"2024","journal-title":"Dig. Surg."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Lluis, N., and Biondo, S. (2018). Prolonged Postoperative Ileus after Colorectal Surgery: Still an Unresolved Problem. Ann. Laparosc. Endosc. Surg., 3.","DOI":"10.21037\/ales.2018.02.06"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Venara, A., Hamel, J.-F., Beyer-Berjot, L., Vignaud, T., Slim, K., Abderrazak, M., Abolo, H., Abras, N., Aissou, M., and Albertini, S. (2021). Link between Postoperative Ileus and Anastomotic Leakage: A Structural Equation Modelling Approach. Surg. Open Dig. Adv., 2.","DOI":"10.1016\/j.soda.2021.100009"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Pavlidis, T.E. (2007). Carcinoma of the Colon Caused Intussusception Presenting with Obstructive Ileus. Int. J. Color. Dis., 22.","DOI":"10.1007\/s00384-005-0746-1"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1111\/codi.13582","article-title":"Relation between Postoperative Ileus and Anastomotic Leakage after Colorectal Resection: A Post Hoc Analysis of a Prospective Randomized Controlled Trial","volume":"19","author":"Peters","year":"2017","journal-title":"Color. Dis."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/j.jss.2012.12.043","article-title":"Tissue Adhesives in Gastrointestinal Anastomosis: A Systematic Review","volume":"180","author":"Vakalopoulos","year":"2013","journal-title":"J. Surg. Res."},{"key":"ref_18","unstructured":"Nordentoft, T. (2015). Sealing of Gastrointestinal Anastomoses with Fibrin Glue Coated Collagen Patch. Dan. Med. J., 62."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Cira, K., Stocker, F., Reischl, S., Obermeier, A., Friess, H., Burgkart, R., and Neumann, P.A. (2022). Coating of Intestinal Anastomoses for Prevention of Postoperative Leakage: A Systematic Review and Meta-Analysis. Front. Surg., 9.","DOI":"10.3389\/fsurg.2022.882173"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"5","DOI":"10.2147\/RMHP.S221008","article-title":"Costs Analysis of Fibrin Sealant for Prevention of Anastomotic Leakage in Lower Colorectal Surgery","volume":"13","author":"Panda","year":"2020","journal-title":"Risk Manag. Healthc. Policy"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"681","DOI":"10.2298\/VSP0809681S","article-title":"Determination of fibrin glue with antibiotics on collagen production in colon anastomosis","volume":"65","year":"2008","journal-title":"Vojnosanit. Pregl."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1001\/archsurg.1988.01400260118015","article-title":"Fibrin Sealant in High-Risk Colonic Anastomoses","volume":"123","author":"Houston","year":"1988","journal-title":"Arch. Surg."},{"key":"ref_23","first-page":"216","article-title":"Fibrin Glue and Coats Compromise the Integrity of Colonic Anastomosis: An Experimental Trial on Rats","volume":"37","author":"Voloudakis","year":"2024","journal-title":"Ann. Gastroenterol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1111\/j.1445-2197.2007.04020.x","article-title":"Surgical Applications of Cyanoacrylate Adhesives: A Review of Toxicity","volume":"77","author":"Leggat","year":"2007","journal-title":"ANZ J. Surg."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1016\/S0003-4975(02)03689-5","article-title":"BioGlue Surgical Adhesive\u2014An Appraisal of Its Indications in Cardiac Surgery","volume":"74","author":"Passage","year":"2002","journal-title":"Ann. Thorac. Surg."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1067\/mtc.2000.108596","article-title":"Sutureless Coronary Artery Bypass with Biologic Glued Anastomoses: Preliminary in Vivo and in Vitro Results","volume":"120","author":"Gundry","year":"2000","journal-title":"J. Thorac. Cardiovasc. Surg."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/S0003-4975(02)04128-0","article-title":"Advances in the Treatment of Acute Type A Dissection: An Integrated Approach","volume":"74","author":"Bavaria","year":"2002","journal-title":"Ann. Thorac. Surg."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1609","DOI":"10.1016\/S0003-4975(01)02424-9","article-title":"BioGlue Surgical Adhesive for Thoracic Aortic Repair during Coagulopathy: Efficacy and Histopathology","volume":"71","author":"Hewitt","year":"2001","journal-title":"Ann. Thorac. Surg."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1048","DOI":"10.1016\/j.athoracsur.2007.01.012","article-title":"Use of Bovine Albumin-Glutaraldehyde Glue in Cardiovascular Surgery","volume":"84","author":"Zehr","year":"2007","journal-title":"Ann. Thorac. Surg."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1016\/S0003-4975(01)02663-7","article-title":"Use of \u201cBioGlue\u201d in Aortic Surgical Repair","volume":"72","author":"Raanani","year":"2001","journal-title":"Ann. Thorac. Surg."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Rathinam, S., Naidu, B.V., Nanjaiah, P., Loubani, M., Kalkat, M.S., and Rajesh, P.B. (2009). BioGlue and Peri-Strips in Lung Volume Reduction Surgery: Pilot Randomised Controlled Trial. J. Cardiothorac. Surg., 4.","DOI":"10.1186\/1749-8090-4-37"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1159\/000356265","article-title":"Use of BioGlue Surgical Adhesive in Hypospadias Repair","volume":"7","author":"Kocherov","year":"2014","journal-title":"Curr. Urol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1590\/S0101-98802007000200005","article-title":"Influ\u00eancia Da Cola Bioglue\u00ae Na Deisc\u00eancia de Anastomose Col\u00f4nica: Estudo Experimental","volume":"27","author":"Mathias","year":"2007","journal-title":"Rev. Bras. De Coloproctol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.9738\/INTSURG-D-15-00085.1","article-title":"Effect of the Bioadhesive, BioGlue, on Impaired Colonic Anastomose Healing in Rats","volume":"100","author":"Ekici","year":"2015","journal-title":"Int. Surg."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Despoudi, K., Mantzoros, I., Ioannidis, O., Loutzidou, L., Christidis, P., Chatzakis, C., Gkasdaris, G., Raptis, D., Pramateftakis, M.G., and Angelopoulos, S. (2021). Healing of Colonic Anastomosis in Rats under Obstructive Ileus Conditions. Discoveries, 9.","DOI":"10.15190\/d.2021.8"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"5680","DOI":"10.3748\/wjg.v23.i31.5680","article-title":"Effects of Albumin\/Glutaraldehyde Glue on Healing of Colonic Anastomosis in Rats","volume":"23","author":"Despoudi","year":"2017","journal-title":"World J. Gastroenterol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1007\/s10350-006-0799-4","article-title":"Evaluation of the Use of BioGlue in the Treatment of High Anal Fistulas: Preliminary Results of a Pilot Study","volume":"50","author":"Rada","year":"2007","journal-title":"Dis. Colon Rectum"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Percie du Sert, N., Hurst, V., Ahluwalia, A., Alam, S., Avey, M.T., Baker, M., Browne, W.J., Clark, A., Cuthill, I.C., and Dirnagl, U. (2020). The ARRIVE Guidelines 2.0: Updated Guidelines for Reporting Animal Research. PLoS Biol., 18.","DOI":"10.1371\/journal.pbio.3000410"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1002\/bjs.1800820410","article-title":"Enhanced Intra-Anastomotic Healing by Operative Lavage with Nutrient Solutions in Experimental Left-Sided Colonic Obstruction","volume":"82","author":"Mathie","year":"1995","journal-title":"Br. J. Surg."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.jss.2014.01.052","article-title":"The Effects of Iloprost on Colonic Anastomotic Healing in Rats under Obstructive Ileus Conditions","volume":"189","author":"Galanopoulos","year":"2014","journal-title":"J. Surg. Res."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1007\/BF02937216","article-title":"An Experimental Single Layer Colonic Anastomosis","volume":"154","author":"Malone","year":"1985","journal-title":"Ir. J. Med. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"239","DOI":"10.6061\/clinics\/2013(02)OA19","article-title":"The Effects of Ghrelin on Colonic Anastomosis Healing in Rats","volume":"68","author":"Ceran","year":"2013","journal-title":"Clinics"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.ijsu.2008.10.005","article-title":"A Comparison of Three Single Layer Anastomotic Techniques in the Colon of the Rat","volume":"7","author":"Krasniqi","year":"2009","journal-title":"Int. J. Surg."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1007\/s101510200033","article-title":"Sutureless Colonic Anastomosis in the Rat: A Randomized Controlled Study","volume":"6","author":"Kanellos","year":"2002","journal-title":"Tech. Coloproctol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1002\/bjs.1800790617","article-title":"Effect of Antibiotics in Fibrin Sealant on Healing Colonic Anastomoses in the Rat","volume":"79","author":"Kort","year":"1992","journal-title":"Br. J. Surg."},{"key":"ref_46","first-page":"255","article-title":"The Effect of Glutamine and Synbiotics on the Healing of Colonic Anastomosis","volume":"106","author":"Sapidis","year":"2014","journal-title":"Rev. Esp. Enfermedades Dig."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Ntampakis, G., Pramateftakis, M.-G., Ioannidis, O., Bitsianis, S., Christidis, P., Symeonidis, S., Koliakos, G., Karakota, M., Bekiari, C., and Tsakona, A. (2023). The Role of Adipose Tissue Mesenchymal Stem Cells in Colonic Anastomosis Healing in Inflammatory Bowel Disease: Experimental Study in Rats. J. Clin. Med., 12.","DOI":"10.3390\/jcm12196336"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/0002-9610(92)90255-P","article-title":"Effects of Chronic Corticosteroids and Vitamin A on the Healing of Intestinal Anastomoses","volume":"163","author":"Phillips","year":"1992","journal-title":"Am. J. Surg."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"413","DOI":"10.26719\/2003.9.3.413","article-title":"Histopathology of Human Intestinal Anastomosis","volume":"9","author":"Shomaf","year":"2003","journal-title":"East. Mediterr. Health J."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"545","DOI":"10.11622\/smedj.2018035","article-title":"Effects of Melatonin on Colonic Anastomosis Healing Following Chemotherapy in Rats","volume":"59","author":"Akyuz","year":"2018","journal-title":"Singap. Med. J."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/0009-9120(96)00003-6","article-title":"A Simplified Method for the Analysis of Hydroxyproline in Biological Tissues","volume":"29","author":"Reddy","year":"1996","journal-title":"Clin. Biochem."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Rosendorf, J., Klicova, M., Herrmann, I., Anthis, A., Cervenkova, L., Palek, R., Treska, V., and Liska, V. (2022). Intestinal Anastomotic Healing: What Do We Know About Processes Behind Anastomotic Complications. Front. Surg., 9.","DOI":"10.3389\/fsurg.2022.904810"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1007\/s00423-020-01957-1","article-title":"Innovative Approaches for Induction of Gastrointestinal Anastomotic Healing: An Update on Experimental and Clinical Aspects","volume":"406","author":"Reischl","year":"2021","journal-title":"Langenbeck\u2019s Arch. Surg."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Morgan, R.B., and Shogan, B.D. (2022). The Science of Anastomotic Healing. Semin. Colon Rectal Surg., 33.","DOI":"10.1016\/j.scrs.2022.100879"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Jongen, A.C., Bosmans, J.W., Kartal, S., Lubbers, T., Sosef, M., Slooter, G.D., Stoot, J.H., van Schooten, F.-J., Bouvy, N.D., and Derikx, J.P. (2016). Predictive Factors for Anastomotic Leakage After Colorectal Surgery: Study Protocol for a Prospective Observational Study (REVEAL Study). JMIR Res. Protoc., 5.","DOI":"10.2196\/resprot.5477"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3111","DOI":"10.3390\/curroncol30030236","article-title":"Predictive Factors for Anastomotic Leakage Following Colorectal Cancer Surgery: Where Are We and Where Are We Going?","volume":"30","author":"Tsalikidis","year":"2023","journal-title":"Curr. Oncol."},{"key":"ref_57","first-page":"37954","article-title":"Prolonged Post-Operative Paralytic Ileus and Anastomotic Leak: Guilty or Not Guilty","volume":"46","year":"2022","journal-title":"Biomed. J. Sci. Tech. Res."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Ntampakis, G., Pramateftakis, M.-G., Anestiadou, E., Bitsianis, S., Ioannidis, O., Bekiari, C., Koliakos, G., Karakota, M., Tsakona, A., and Cheva, A. (2024). Experimental Models of High-Risk Bowel Anastomosis in Rats: A Systematic Review. World J. Exp. Med., 14.","DOI":"10.5493\/wjem.v14.i2.94135"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1393","DOI":"10.1007\/s00384-018-3132-5","article-title":"Ileus Caused by Obstructing Colorectal Cancer\u2014Impact on Long-Term Survival","volume":"33","author":"Boeding","year":"2018","journal-title":"Int. J. Color. Dis."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"e3","DOI":"10.1016\/j.jviscsurg.2011.11.003","article-title":"Emergency Management of Acute Colonic Cancer Obstruction","volume":"149","author":"Gainant","year":"2012","journal-title":"J. Visc. Surg."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Ioannidis, O., Ramirez, J.M., Ubieto, J.M., Feo, C.V., Arroyo, A., Koci\u00e1n, P., S\u00e1nchez-Guill\u00e9n, L., Bellosta, A.P., Whitley, A., and Enguita, A.B. (2023). The EUPEMEN (EUropean PErioperative MEdical Networking) Protocol for Bowel Obstruction: Recommendations for Perioperative Care. J. Clin. Med., 12.","DOI":"10.20944\/preprints202304.1200.v1"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1212","DOI":"10.1023\/B:DDAS.0000037815.63547.08","article-title":"Structural and Neuronal Changes in Rat Ileum after Ischemia with Reperfusion","volume":"49","author":"Ekblad","year":"2004","journal-title":"Dig. Dis. Sci."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"70","DOI":"10.9738\/INTSURG-D-13-00191.1","article-title":"Impaired Blood Supply in the Colonic Anastomosis in Mice Compromises Healing","volume":"100","author":"Pommergaard","year":"2015","journal-title":"Int. Surg."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1179\/135100007X239261","article-title":"Colonic Healing after Portal Ischemia and Reperfusion: An Experimental Study with Oxidative Stress Biomarkers","volume":"12","author":"Riger","year":"2007","journal-title":"Redox Rep."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/s00423-010-0726-y","article-title":"Intestinal Ischemia: Current Treatment Concepts","volume":"396","author":"Renner","year":"2011","journal-title":"Langenbeck\u2019s Arch. Surg."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"2283","DOI":"10.2353\/ajpath.2010.091069","article-title":"Human Intestinal Ischemia-Reperfusion-Induced Inflammation Characterized: Experiences from a New Translational Model","volume":"176","author":"Grootjans","year":"2010","journal-title":"Am. J. Pathol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"3158","DOI":"10.1007\/s10620-008-0279-x","article-title":"Infiltration of Mast Cells in Rat Colon Is a Consequence of Ischemia\/Reperfusion","volume":"53","author":"Sand","year":"2008","journal-title":"Dig. Dis. Sci."},{"key":"ref_68","first-page":"5","article-title":"Our 20-Year Experience with Experimental Colonic Anastomotic Healing","volume":"11","author":"Raptis","year":"2018","journal-title":"J. Med. Life"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1007\/s00268-006-0094-5","article-title":"Effect of 5-Fluorouracil plus Interferon on the Integrity of Colonic Anastomoses Covering with Fibrin Glue","volume":"31","author":"Kanellos","year":"2007","journal-title":"World J. Surg."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.jss.2013.10.013","article-title":"Growth Factors and Gastrointestinal Anastomotic Healing","volume":"187","author":"Rijcken","year":"2014","journal-title":"J. Surg. Res."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1007\/s00384-016-2661-z","article-title":"Gastrointestinal Tract Anastomoses with the Biofragmentable Anastomosis Ring: Is It Still a Valid Technique for Bowel Anastomosis? Analysis of 203 Cases and Review of the Literature","volume":"32","author":"Bobkiewicz","year":"2017","journal-title":"Int. J. Color. Dis."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"267","DOI":"10.5761\/atcs.nm.20-00198","article-title":"Aortic Root Reconstruction with Tachosil Fibrin Sealant Patch in Acute Type a Aortic Dissection","volume":"27","author":"Bis","year":"2021","journal-title":"Ann. Thorac. Cardiovasc. Surg."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/j.ygyno.2019.01.011","article-title":"The Application of Fibrin Sealant for the Prevention of Lymphocele after Lymphadenectomy in Patients with Gynecological Malignancies: A Systematic Review and Meta-Analysis of Randomized Controlled Trials","volume":"153","author":"Prodromidou","year":"2019","journal-title":"Gynecol. Oncol."},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Nerstr\u00f8m, M., Krarup, P.-M., Jorgensen, L.N., and \u00c5gren, M.S. (2016). Therapeutic Improvement of Colonic Anastomotic Healing under Complicated Conditions: A Systematic Review. World J. Gastrointest. Surg., 8.","DOI":"10.4240\/wjgs.v8.i5.389"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1007\/s005950050615","article-title":"The Effect of Intraoperative Colonic Lavage with NG-Nitro-L-Arginine Methyl Ester (L-NAME) on Anastomotic Healing in the Presence of Left-Sided Colonic Obstruction in the Rat","volume":"30","author":"Erbil","year":"2000","journal-title":"Surg. Today"},{"key":"ref_76","first-page":"530","article-title":"The Healing Role of Erythropoietin in the Obstructive vs. Nonobstructive Left Colonic Anastomosis","volume":"110","author":"Faruquzzaman","year":"2009","journal-title":"Bratisl. Lek. Listy"},{"key":"ref_77","first-page":"213","article-title":"Locally Applied Molgramostim Improves Wound Healing at Colonic Anastomoses in Rats after Ligation of the Common Bile Duct","volume":"48","author":"Gulcelik","year":"2005","journal-title":"Can. J. Surg."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1177\/1756287217697662","article-title":"Bovine Serum Albumin-Glutaraldehyde (BioGlue\u00ae) Tissue Adhesive versus Standard Renorrhaphy Following Renal Mass Enucleation: A Retrospective Comparison","volume":"9","author":"Bahouth","year":"2017","journal-title":"Ther. Adv. Urol."},{"key":"ref_79","doi-asserted-by":"crossref","unstructured":"Abbas, M.A., and Tejirian, T. (2008). Bioglue for the Treatment of Anal Fistula Is Associated with Acute Anal Sepsis. Dis. Colon Rectum, 51.","DOI":"10.1007\/s10350-008-9332-2"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1007\/s10151-010-0674-0","article-title":"Protection of Intestinal Anastomosis with Biological Glues: An Experimental Randomized Controlled Trial","volume":"15","author":"Giuratrabocchetta","year":"2011","journal-title":"Tech. Coloproctol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1016\/j.fertnstert.2007.12.006","article-title":"BioGlue and Dermabond Save Time, Leak Less, and Are Not Mechanically Inferior to Two-Layer and Modified One-Layer Vasovasostomy","volume":"91","author":"Saunders","year":"2009","journal-title":"Fertil. Steril."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1111\/j.1540-8191.2007.00410.x","article-title":"BioGlue\u00ae: A Protective Barrier after Pericardiotomy","volume":"22","author":"Wang","year":"2007","journal-title":"J. Card. Surg."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1007\/s11845-008-0225-0","article-title":"The \u201cFlying\u201d Bile Duct: Avulsion of the Common Bile Duct in a Plane Crash Survivor","volume":"178","author":"Mohan","year":"2009","journal-title":"Ir. J. Med. Sci."},{"key":"ref_84","first-page":"1106","article-title":"Mechanical and Chemical Characteristics of an Autologous Glue","volume":"86","author":"Delanghe","year":"2008","journal-title":"J. Biomed. Mater. Res.-Part A"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1001\/archsurg.137.3.326","article-title":"A Polymeric Sealant Inhibits Anastomotic Suture Hole Bleeding More Rapidly than Gelfoam\/Thrombin: Results of a Randomized Controlled Trial","volume":"137","author":"Glickman","year":"2002","journal-title":"Arch. Surg."},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Chen, X., Qin, R., Liu, B., Ma, Y., Su, Y., Yang, C.S., Glickman, J.N., Odze, R.D., and Shaheen, N.J. (2008). Multilayered Epithelium in a Rat Model and Human Barrett\u2019s Esophagus: Similar Expression Patterns of Transcription Factors and Differentiation Markers. BMC Gastroenterol., 8.","DOI":"10.1186\/1471-230X-8-1"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/j.surneu.2006.06.043","article-title":"BioGlue\u00ae for Prevention of Postoperative Cerebrospinal Fluid Leaks in Transsphenoidal Surgery: A Case Series","volume":"66","author":"Dusick","year":"2006","journal-title":"Surg. Neurol."},{"key":"ref_88","first-page":"36","article-title":"Malnutrition Is Associated with Adverse Postoperative Outcome in Patients Undergoing Elective Colorectal Cancer Resections","volume":"23","author":"Seretis","year":"2018","journal-title":"J. BUON"},{"key":"ref_89","doi-asserted-by":"crossref","unstructured":"Sripathi, S., Khan, M.I., Patel, N., Meda, R.T., Nuguru, S.P., and Rachakonda, S. (2022). Factors Contributing to Anastomotic Leakage Following Colorectal Surgery: Why, When, and Who Leaks?. Cureus, 14.","DOI":"10.7759\/cureus.29964"},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1007\/s00384-017-2857-x","article-title":"Selective Matrix Metalloproteinase Inhibition Increases Breaking Strength and Reduces Anastomotic Leakage in Experimentally Obstructed Colon","volume":"32","author":"Krarup","year":"2017","journal-title":"Int. J. Color. Dis."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1007\/s00384-011-1337-y","article-title":"The Effects of Tacrolimus on Colonic Anastomotic Healing in Rats","volume":"27","author":"Raptis","year":"2012","journal-title":"Int. J. Color. Dis."},{"key":"ref_92","doi-asserted-by":"crossref","unstructured":"Ghiselli, R., Lucarini, G., Ortenzi, M., Salvolini, E., Saccomanno, S., Orlando, F., Provinciali, M., Casciani, F., and Guerrieri, M. (2020). Anastomotic Healing in a Rat Model of Peritonitis after Non-Steroidal Anti-Inflammatory Drug Administration. Eur. J. Histochem., 64.","DOI":"10.4081\/ejh.2020.3085"},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.1007\/s00384-012-1435-5","article-title":"The Effect of Fibrin Glue on the Early Healing Phase of Intestinal Anastomoses in the Rat","volume":"27","author":"Lomme","year":"2012","journal-title":"Int. J. Color. Dis."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1111\/j.1067-1927.2005.130102.x","article-title":"Tissue Repair, Contraction, and the Myofibroblast","volume":"13","author":"Chaponnier","year":"2005","journal-title":"Wound Repair Regen."},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1590\/S0100-879X2001000400012","article-title":"Effect of Sodium Carboxymethylcellulose and Methylprednisolone on the Healing of Jejunal Anastomoses in Rats","volume":"34","author":"Sousa","year":"2001","journal-title":"Braz. J. Med. Biol. Res."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"221","DOI":"10.21873\/invivo.12694","article-title":"Beneficiary Effect of Fibrin Glue on Healing of Ileoileal Anastomoses in Rats","volume":"36","author":"Gerogiannis","year":"2022","journal-title":"In Vivo"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1111\/j.0906-6705.2004.00233.x","article-title":"The Macrophage-Activating Lipopeptide-2 Accelerates Wound Healing in Diabetic Mice","volume":"13","author":"Deiters","year":"2004","journal-title":"Exp. Dermatol."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1002\/jbm.b.33621","article-title":"Clinical, Mechanical, and Immunohistopathological Effects of Tissue Adhesives on the Colon: An in-Vivo Study","volume":"105","author":"Vakalopoulos","year":"2017","journal-title":"J. Biomed. Mater. Res.-Part B Appl. Biomater."},{"key":"ref_99","first-page":"149","article-title":"Angiogenic and Angiostatic Factors in the Molecular Control of Angiogenesis","volume":"47","author":"Distler","year":"2003","journal-title":"Q. J. Nucl. Med."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1590\/S0102-86502012001000007","article-title":"Effects of Chitosan on Healing and Strength of Colonic Anastomosis in Rats|Efeitos de Quitosana Na Cicatriza\u00e7\u00e3o e Resist\u00eancia de Anastomose Col\u00f4nica Em Ratos","volume":"27","author":"Citgez","year":"2012","journal-title":"Acta Cir. Bras."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"659","DOI":"10.21873\/invivo.11820","article-title":"The Effect of TISSEEL\u00ae on Colorectal Anastomosis Healing Process in a Diabetic Animal Experimental Model","volume":"34","author":"Stergios","year":"2020","journal-title":"In Vivo"},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.jss.2007.05.015","article-title":"Effects of Platelet Rich Plasma on Colonic Anastomosis","volume":"146","author":"Yol","year":"2008","journal-title":"J. Surg. Res."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"2290","DOI":"10.1111\/ans.15993","article-title":"Effect of Platelet-Rich Plasma on Colon Anastomosis in Rats in Which Hyperthermic Intra-Peritoneal Chemotherapy Was Performed Using 5-Fluorouracil","volume":"90","author":"Ertan","year":"2020","journal-title":"ANZ J. Surg."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"1284","DOI":"10.1002\/bjs.1800771129","article-title":"Direct Measurement of Collagenase in Colonic Anastomosis","volume":"77","author":"Chowcat","year":"1990","journal-title":"Br. J. Surg."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1016\/S0003-4975(01)03524-X","article-title":"Inflammatory Response after BioGlue Application","volume":"73","author":"Erasmi","year":"2002","journal-title":"Ann. Thorac. Surg."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1016\/j.jss.2013.04.015","article-title":"Prevention of Leakage by Sealing Colon Anastomosis: Experimental Study in a Mouse Model","volume":"184","author":"Slieker","year":"2013","journal-title":"J. Surg. Res."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1159\/000366418","article-title":"Fibrin Glue Does Not Improve Healing of Gastrointestinal Anastomoses: A Systematic Review","volume":"54","author":"Nordentoft","year":"2015","journal-title":"Eur. Surg. Res."},{"key":"ref_108","unstructured":"Petrie, E.M. (2018). Cyanoacrylate Adhesives in Surgical Applications Cyanoacrylate Adhesives in Surgical Applications: A Critical Review. Progress in Adhesion and Adhesives, Wiley."}],"container-title":["Journal of Clinical Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2077-0383\/14\/7\/2457\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:09:38Z","timestamp":1760029778000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2077-0383\/14\/7\/2457"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,3]]},"references-count":108,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2025,4]]}},"alternative-id":["jcm14072457"],"URL":"https:\/\/doi.org\/10.3390\/jcm14072457","relation":{},"ISSN":["2077-0383"],"issn-type":[{"value":"2077-0383","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,3]]}}}