{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T17:14:28Z","timestamp":1784740468435,"version":"3.55.0"},"reference-count":31,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T00:00:00Z","timestamp":1744329600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Background: We analyzed the performance of a multiplex PCR application (Unyvero IAI) in comparison to culture in a cohort of peritonitis patients undergoing peritoneal dialysis or with liver cirrhosis. Methods: We performed a single-center study of 47 patients with clinically suspected peritonitis and compared pathogen detection rates of culture and PCR. The main outcome of interest was a comparison of accuracy and time to final positive result. Results: In the total cohort, the pathogen detection rate in culture was 58.14% versus 34.88% in PCR (p = 0.03). Detection rates of bacteria in peritoneal dialysis patients were even higher by culture (70.83%) but comparably low by PCR (37.50%; p = 0.04). The majority of discordant results were in the Gram-positive spectrum (81.82%). Differential time to final positive result was 37.39 \u00b1 16.75 h. Conclusion: Time gains by using PCR diagnostic have to be weighed against lower detection rates, mainly in Gram-positive infections.<\/jats:p>","DOI":"10.3390\/jcm14082641","type":"journal-article","created":{"date-parts":[[2025,4,14]],"date-time":"2025-04-14T04:42:07Z","timestamp":1744605727000},"page":"2641","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Multiplex PCR for Microbiological Testing in Patients with Peritoneal Dialysis- and Liver Cirrhosis-Related Peritonitis: Faster, but Less Sensitive"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7504-4436","authenticated-orcid":false,"given":"Sebastian","family":"Schwab","sequence":"first","affiliation":[{"name":"Department of Internal Medicine I, University of Bonn, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"P\u00f6rner","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, University of Bonn, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominik","family":"Boes","sequence":"additional","affiliation":[{"name":"Kuratorium for Dialysis, KfH Renal Center, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8671-3831","authenticated-orcid":false,"given":"Achim","family":"Hoerauf","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, Immunology and Parasitology, University Hospital of Bonn, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob","family":"Nattermann","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, University of Bonn, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Strassburg","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, University of Bonn, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gunnar T. R.","family":"Hischebeth","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, Immunology and Parasitology, University Hospital of Bonn, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4921-200X","authenticated-orcid":false,"given":"Philipp","family":"Lutz","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I, University of Bonn, 53127 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Bach, N., Chi, T.T.K., Trung, L.C., Ngoc, N.H.B., Hoa, T.P.T., Huynh, T.N.P., Khai, T.H., Dao, D.A., Tuoc, P.X., and Dung, V.Q. (2025). Prevalence, microbiology, and outcome of peritonitis in peritoneal dialysis patients in vietnam: A multicenter study. 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