{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T19:42:49Z","timestamp":1792698169878,"version":"4.5.0"},"reference-count":15,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,6,19]],"date-time":"2025-06-19T00:00:00Z","timestamp":1750291200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Objectives: A Ziemer LDV Z8 femtosecond laser (FSL) was used to obtain optimal cutting parameters with precise settings for donor and recipient preparations for Bowman layer transplantation. Methods: Of 48 human research corneas examined, 32 were used for Bowman layer preparation (donor) and 16 for pocket preparation (recipient) using the LDV Z8 FSL. The cutting thickness of the Bowman layer, pocket depth, and corresponding laser settings were varied. The quality of sections was evaluated based on the occurrence of adhesions, bridges, or perforations. Histological specimens were prepared and analyzed. Results: Preparation of the Bowman layer and recipient pocket was possible using all selected settings. The thinner the Bowman layer and the more superficial the pocket preparation, the higher the risk of perforation was. Considering the fact that the Bowman layer was cut as thinly as possible, a Bowman layer thickness of 30 \u00b5m showed a 100% success rate. Bowman layers cut at 25 \u00b5m had a lower success rate (50%). The pocket depth of 150 \u00b5m showed a 100% success rate in the preparation. Histological processing revealed smooth, precisely cut edges of Bowman layers and pockets. Implantation into the pocket was successful in all cases. Conclusions: Both Bowman layer and pocket preparation were technically and surgically feasible using the LDV Z8 FSL, and the prepared Bowman layers were thinner than those reported in previous studies. The optimal Bowman layer thickness was 30 \u00b5m, and a resection depth of 150 \u00b5m was used to prepare the pockets safely.<\/jats:p>","DOI":"10.3390\/jcm14124362","type":"journal-article","created":{"date-parts":[[2025,6,19]],"date-time":"2025-06-19T06:11:07Z","timestamp":1750313467000},"page":"4362","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Femtosecond Laser-Assisted Donor and Recipient Preparation for Bowman Layer Transplantation"],"prefix":"10.3390","volume":"14","author":[{"given":"Rosemarie","family":"Schlosser","sequence":"first","affiliation":[{"name":"Eye Clinic Sulzbach, Knappschaft Hospital Saar, 66280 Sulzbach, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annekatrin","family":"Rickmann","sequence":"additional","affiliation":[{"name":"Eye Clinic Sulzbach, Knappschaft Hospital Saar, 66280 Sulzbach, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Szurman","sequence":"additional","affiliation":[{"name":"Eye Clinic Sulzbach, Knappschaft Hospital Saar, 66280 Sulzbach, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silke","family":"Wahl","sequence":"additional","affiliation":[{"name":"Eye Clinic Sulzbach, Knappschaft Hospital Saar, 66280 Sulzbach, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9701-8204","authenticated-orcid":false,"given":"Berthold","family":"Seitz","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Saarland University Medical Center, 66421 Homburg\/Saar, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3587-6382","authenticated-orcid":false,"given":"Philip","family":"Wakili","sequence":"additional","affiliation":[{"name":"Eye Clinic Sulzbach, Knappschaft Hospital Saar, 66280 Sulzbach, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0198-9255","authenticated-orcid":false,"given":"Lisa Julia","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Eye Clinic Sulzbach, Knappschaft Hospital Saar, 66280 Sulzbach, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3703-4055","authenticated-orcid":false,"given":"Philipp Ken","family":"Roberts","sequence":"additional","affiliation":[{"name":"Eye Clinic Sulzbach, Knappschaft Hospital Saar, 66280 Sulzbach, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0277-2525","authenticated-orcid":false,"given":"Karl Thomas","family":"Boden","sequence":"additional","affiliation":[{"name":"Eye Clinic Sulzbach, Knappschaft Hospital Saar, 66280 Sulzbach, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1055\/s-2005-859021","article-title":"Keratokonus: Epidemiologie, Risikofaktoren und Diagnostik","volume":"223","author":"Duncker","year":"2006","journal-title":"Klin. Monatsblatter Fur Augenheilkd."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.1136\/bjo.2010.185868","article-title":"Management of keratoconus: Current scenario","volume":"95","author":"Jhanji","year":"2011","journal-title":"Br. J. Ophthalmol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.1016\/j.jcrs.2010.03.032","article-title":"Isolated Bowman layer transplantation to manage persistent subepithelial haze after excimer laser surface ablation","volume":"36","author":"Lie","year":"2010","journal-title":"J. Cataract Refract. Surg."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1016\/j.survophthal.2015.02.004","article-title":"Treatment options for advanced keratoconus: A review","volume":"60","author":"Parker","year":"2015","journal-title":"Surv. Ophthalmol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1097\/ICU.0000000000000665","article-title":"Potential benefits of modified corneal tissue grafts for keratoconus: Bowman layer \u2018inlay\u2019 and \u2018onlay\u2019 transplantation, and allogenic tissue ring segments","volume":"31","author":"Dapena","year":"2020","journal-title":"Curr. Opin. Ophthalmol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1097\/ICO.0000000000000860","article-title":"Corneal Densitometry and Higher Order Aberrations After Bowman Layer Transplantation: 1-Year Results","volume":"35","author":"Luceri","year":"2016","journal-title":"Cornea"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"675","DOI":"10.5301\/ejo.5000990","article-title":"Technical feasibility of isolated Bowman layer graft preparation by femtosecond laser: A pilot study","volume":"27","author":"Parker","year":"2017","journal-title":"Eur. J. Ophthalmol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"433","DOI":"10.2147\/OPTH.S141127","article-title":"Bowman\u2019s layer transplantation: Evidence to date","volume":"12","author":"Sharma","year":"2018","journal-title":"Clin. Ophthalmol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.ajo.2008.08.026","article-title":"Femtosecond lasers in ophthalmology","volume":"147","author":"Soong","year":"2009","journal-title":"Am. J. Ophthalmol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Cerroni, L., Garbe, C., Metze, D., Kutzner, H., and Kerl, H. (2016). Histologische Techniken. Histopathologie der Haut, Springer.","DOI":"10.1007\/978-3-662-45133-5"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1001\/jamaophthalmol.2013.5841","article-title":"Midstromal isolated Bowman layer graft for reduction of advanced keratoconus: A technique to postpone penetrating or deep anterior lamellar keratoplasty","volume":"132","author":"Parker","year":"2014","journal-title":"JAMA Ophthalmol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.jcrs.2018.10.034","article-title":"Bowman layer transplantation using a femtosecond laser","volume":"45","author":"Alquisiras","year":"2019","journal-title":"J. Cataract Refract. Surg."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1097\/APO.0000000000000336","article-title":"Bowman Layer Transplantation-A Review","volume":"9","author":"Parker","year":"2020","journal-title":"Asia-Pac. J. Ophthalmol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1055\/s-0032-1328257","article-title":"Immunreaktionen nach DMEK, DSAEK und DALK","volume":"230","author":"Steven","year":"2013","journal-title":"Klin. Monatsblatter Fur Augenheilkd."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Aggarwal, S., Kumari, M., and Bhatnagar, N. (2024). Advancements in keratoplasty: Exploring newer techniques and imaging modalities for enhanced surgical outcomes. Saudi J. Ophthalmol.","DOI":"10.4103\/sjopt.sjopt_234_23"}],"container-title":["Journal of Clinical Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2077-0383\/14\/12\/4362\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:54:44Z","timestamp":1760032484000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2077-0383\/14\/12\/4362"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,19]]},"references-count":15,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2025,6]]}},"alternative-id":["jcm14124362"],"URL":"https:\/\/doi.org\/10.3390\/jcm14124362","relation":{},"ISSN":["2077-0383"],"issn-type":[{"value":"2077-0383","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,19]]}}}