{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T12:01:12Z","timestamp":1780401672395,"version":"3.54.1"},"reference-count":58,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T00:00:00Z","timestamp":1751932800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Assessment for the presence or absence of lupus anticoagulant (LA) represents a common investigation in hemostasis laboratories. In particular, LA represents one of the laboratory criteria for the diagnosis of definite antiphospholipid syndrome (APS). The other laboratory criteria are the solid phase assays (anticardiolipin (aCL) and anti-\u03b22Glycoprotein I (a\u03b22GPI) antibodies of IgG and IgM isotypes). Current International Society on Thrombosis and Haemostasis (ISTH) guidance recommends testing LA by at least two tests based on different principles, with the activated partial thromboplastin time (aPTT) and dilute Russell viper venom time (dRVVT) being preferred. Additional assays may be used in addition, or instead of these assays in particular situations. For example, aPTT and dRVVT assays are very sensitive to the presence of various anticoagulants, and this may lead to false-positive identification of LA. This is particularly problematic in the age of the DOACs (direct oral anticoagulants), which are now the leading anticoagulants in use worldwide. We review recent literature on LA testing as well as our local practice to provide an update on this common test procedure. Our experience should be useful for laboratories struggling with LA interpretation for diagnosis or exclusion of APS.<\/jats:p>","DOI":"10.3390\/jcm14144812","type":"journal-article","created":{"date-parts":[[2025,7,8]],"date-time":"2025-07-08T02:25:53Z","timestamp":1751941553000},"page":"4812","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Lupus Anticoagulant Testing for Diagnosis of Antiphospholipid Syndrome: A Perspective Informed by Local Practice"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2103-1661","authenticated-orcid":false,"given":"Emmanuel J.","family":"Favaloro","sequence":"first","affiliation":[{"name":"Haematology, Sydney Centres for Thrombosis and Haemostasis, Institute of Clinical Pathology and Medical Research (ICPMR), NSW Health Pathology, Westmead Hospital, Westmead, NSW 2145, Australia"},{"name":"Sydney Centres for Thrombosis and Haemostasis, Research and Education Network, Westmead Hospital, Westmead, NSW 2145, Australia"},{"name":"School of Dentistry and Medical Sciences, Faculty of Science and Health, Charles Sturt University, Wagga Wagga, NSW 2650, Australia"},{"name":"School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, Westmead Hospital, Westmead, NSW 2145, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8237-6743","authenticated-orcid":false,"given":"Leonardo","family":"Pasalic","sequence":"additional","affiliation":[{"name":"Haematology, Sydney Centres for Thrombosis and Haemostasis, Institute of Clinical Pathology and Medical Research (ICPMR), NSW Health Pathology, Westmead Hospital, Westmead, NSW 2145, Australia"},{"name":"Sydney Centres for Thrombosis and Haemostasis, Research and Education Network, Westmead Hospital, Westmead, NSW 2145, Australia"},{"name":"Westmead Clinical School, University of Sydney, Westmead, NSW 2145, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.1136\/ard-2023-224609","article-title":"2023 ACR\/EULAR antiphospholipid syndrome classification criteria","volume":"82","author":"Barbhaiya","year":"2023","journal-title":"Ann. Rheum. Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1111\/j.1538-7836.2006.01753.x","article-title":"International consensus statement on an update of the classification criteria for definite antiphospholipid syndrome (APS)","volume":"4","author":"Miyakis","year":"2006","journal-title":"J. Thromb. Haemost."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1002\/1529-0131(199907)42:7<1309::AID-ANR1>3.0.CO;2-F","article-title":"International consensus statement on preliminary classification criteria for definite antiphospholipid syndrome: Report of an international workshop","volume":"42","author":"Wilson","year":"1999","journal-title":"Arthritis Rheum."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1055\/s-0043-1776318","article-title":"Classification Criteria for the Antiphospholipid Syndrome: Not the Same as Diagnostic Criteria for Antiphospholipid Syndrome","volume":"50","author":"Favaloro","year":"2024","journal-title":"Semin. Thromb. Hemost."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1016\/j.jtha.2024.10.022","article-title":"An update on laboratory detection and interpretation of antiphospholipid antibodies for diagnosis of antiphospholipid syndrome: Guidance from the International Society on Thrombosis and Haemostasis Scientific and Standardization Committee (ISTH-SSC) Subcommittee on Lupus Anticoagulant\/Antiphospholipid Antibodies","volume":"23","author":"Devreese","year":"2025","journal-title":"J. Thromb. Haemost."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"102735","DOI":"10.1016\/j.rpth.2025.102735","article-title":"2023 American College of Rheumatology\/European Alliance of Associations for Rheumatology classification criteria for antiphospholipid syndrome: Good for patients or good for papers?","volume":"9","author":"Devreese","year":"2025","journal-title":"Res. Pract. Thromb. Haemost."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2828","DOI":"10.1111\/jth.15047","article-title":"Guidance from the Scientific and Standardization Committee for lupus anticoagulant\/antiphospholipid antibodies of the International Society on Thrombosis and Haemostasis: Update of the guidelines for lupus anticoagulant detection and interpretation","volume":"18","author":"Devreese","year":"2020","journal-title":"J. Thromb. Haemost."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1400","DOI":"10.1002\/art.40930","article-title":"2019 European League Against Rheumatism\/American College of Rheumatology Classification Criteria for Systemic Lupus Erythematosus","volume":"71","author":"Aringer","year":"2019","journal-title":"Arthritis Rheumatol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"e001674","DOI":"10.1136\/lupus-2025-001674","article-title":"Impact of antiphospholipid antibodies on heart valve involvements in systemic lupus erythematosus: Based on CSTAR cohort","volume":"12","author":"Chen","year":"2025","journal-title":"Lupus Sci. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e001471","DOI":"10.1136\/lupus-2024-001471","article-title":"Pulmonary arterial hypertension in systemic lupus erythematosus: Identification of risk factors and haemodynamics characteristics in a multicentre retrospective cohort","volume":"12","author":"Marasco","year":"2025","journal-title":"Lupus Sci. Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2287","DOI":"10.1007\/s10067-025-07411-1","article-title":"Effects of telitacicept in SLE patients with antiphospholipid antibody positivity: A retrospective self-controlled case series","volume":"44","author":"Zhou","year":"2025","journal-title":"Clin. Rheumatol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1159\/000205123","article-title":"An unusual circulating anticoagulant in systemic lupus erythematosus","volume":"16","author":"Ramot","year":"1956","journal-title":"Acta Haematol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1001\/archderm.1956.01550090070014","article-title":"A serum anticoagulant factor in systemic lupus erythematosus","volume":"74","author":"Swift","year":"1956","journal-title":"AMA Arch. Derm."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1737","DOI":"10.1111\/j.1538-7836.2009.03555.x","article-title":"Update of the guidelines for lupus anticoagulant detection. Subcommittee on Lupus Anticoagulant\/Antiphospholipid Antibody of the Scientific and Standardisation Committee of the International Society on Thrombosis and Haemostasis","volume":"7","author":"Pengo","year":"2009","journal-title":"J. Thromb. Haemost."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1055\/s-0038-1649901","article-title":"Criteria for the diagnosis of lupus anticoagulants: An update. On behalf of the Subcommittee on Lupus Anticoagulant\/Antiphospholipid Antibody of the Scientific and Standardisation Committee of the ISTH","volume":"74","author":"Brandt","year":"1995","journal-title":"Thromb. Haemost."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3117","DOI":"10.1111\/jth.15056","article-title":"Additional laboratory tests to improve on the diagnosis of antiphospholipid syndrome","volume":"18","author":"Tripodi","year":"2020","journal-title":"J. Thromb. Haemost."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Bradacova, P., Slavik, L., Ulehlova, J., Skoumalova, A., Ullrychova, J., Prochazkova, J., Hlusi, A., Manukyan, G., and Kriegova, E. (2021). Current Promising Biomarkers and Methods in the Diagnostics of Antiphospholipid Syndrome: A Review. Biomedicines, 9.","DOI":"10.3390\/biomedicines9020166"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1111\/ijlh.14240","article-title":"Taipan snake venom time has high sensitivity for lupus anticoagulants in non-anticoagulated, triple positive antiphospholipid syndrome patients","volume":"46","author":"Moore","year":"2024","journal-title":"Int. J. Lab. Hematol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1007\/978-1-0716-3175-1_16","article-title":"Lupus Anticoagulant Testing: Taipan Snake Venom Time with Ecarin Time as Confirmatory Test","volume":"2663","author":"Moore","year":"2023","journal-title":"Methods Mol. Biol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1055\/s-0043-1776406","article-title":"Variable Performance of Lupus Anticoagulant Testing: The Australasian\/Asia-Pacific Experience","volume":"50","author":"Favaloro","year":"2024","journal-title":"Semin. Thromb. Hemost."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Tripodi, A. (2021). Diagnostic Challenges on the Laboratory Detection of Lupus Anticoagulant. Biomedicines, 9.","DOI":"10.3390\/biomedicines9070844"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1111\/ijlh.13195","article-title":"Testing for antiphospholipid antibodies: Advances and best practices","volume":"42","author":"Devreese","year":"2020","journal-title":"Int. J. Lab. Hematol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Xie, H., Sun, Q., Liu, M., Xu, Y., Wu, Q., and Li, D. (2025). Clinical Features of Antiphospholipid Syndrome with Intracardiac Mass. Semin. Thromb. Hemost.","DOI":"10.1055\/a-2561-0149"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Murvai, V.R., Gali\u0219, R., Panaitescu, A., Radu, C.M., Ghitea, T.C., Trif, P., Oni\u021ba-Avram, M., Vesa, A.A., and Huniadi, A. (2025). Antiphospholipid syndrome in pregnancy: A comprehensive literature review. BMC Pregnancy Childbirth, 25.","DOI":"10.1186\/s12884-025-07471-w"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Devreese, K.M.J. (2024). Thrombosis in Antiphospholipid Syndrome: Current Perspectives and Challenges in Laboratory Testing for Antiphospholipid Antibodies. Semin. Thromb. Hemost.","DOI":"10.1055\/s-0044-1791699"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1515\/cclm-2024-0114","article-title":"Patients with antiphospholipid syndrome and a first venous or arterial thrombotic event: Clinical characteristics, antibody profiles and estimate of the risk of recurrence","volume":"62","author":"Pengo","year":"2024","journal-title":"Clin. Chem. Lab. Med."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1182\/blood.2023020727","article-title":"How I diagnose and treat antiphospholipid syndrome in pregnancy","volume":"143","author":"Branch","year":"2024","journal-title":"Blood"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Bernardi, M., Spadafora, L., Andaloro, S., Piscitelli, A., Fornaci, G., Intonti, C., Fratta, A.E., Hsu, C.E., Kazir\u00f2d-Wolski, K., and Metsovitis, T. (2024). Management of Cardiovascular Complications in Antiphospholipid Syndrome: A Narrative Review with a Focus on Older Adults. J. Clin. Med., 13.","DOI":"10.3390\/jcm13113064"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"10760296241306751","DOI":"10.1177\/10760296241306751","article-title":"Key Issues at the Forefront of Diagnosis and Testing for Antiphospholipid Syndrome","volume":"30","author":"Qiao","year":"2024","journal-title":"Clin. Appl. Thromb. Hemost."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e12676","DOI":"10.1002\/rth2.12676","article-title":"Lupus anticoagulant testing during anticoagulation, including direct oral anticoagulants","volume":"6","author":"Favaloro","year":"2022","journal-title":"Res. Pract. Thromb. Haemost."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Tripodi, A., Scalambrino, E., Clerici, M., and Peyvandi, F. (2023). Laboratory Diagnosis of Antiphospholipid Syndrome in Anticoagulated Patients. Biomedicines, 11.","DOI":"10.3390\/biomedicines11061760"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1111\/ijlh.13342","article-title":"Comprehensive review of the impact of direct oral anticoagulants on thrombophilia diagnostic tests: Practical recommendations for the laboratory","volume":"43","author":"Siriez","year":"2021","journal-title":"Int. J. Lab. Hematol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/j.pathol.2018.11.008","article-title":"Laboratory testing for lupus anticoagulant (LA) in patients taking direct oral anticoagulants (DOACs): Potential for false positives and false negatives","volume":"51","author":"Favaloro","year":"2019","journal-title":"Pathology"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1055\/s-0041-1729856","article-title":"Is Lupus Anticoagulant a Significant Feature of COVID-19? A Critical Appraisal of the Literature","volume":"48","author":"Favaloro","year":"2022","journal-title":"Semin. Thromb. Hemost."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2003","DOI":"10.1111\/jth.14894","article-title":"Direct oral anticoagulant adsorption: Impact on lupus anticoagulant testing-Review of the literature and evaluation on spiked and patient samples","volume":"18","author":"Devreese","year":"2020","journal-title":"J. Thromb. Haemost."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1055\/s-0038-1677018","article-title":"How to Optimize Activated Partial Thromboplastin Time (APTT) Testing: Solutions to Establishing and Verifying Normal Reference Intervals and Assessing APTT Reagents for Sensitivity to Heparin, Lupus Anticoagulant, and Clotting Factors","volume":"45","author":"Favaloro","year":"2019","journal-title":"Semin. Thromb. Hemost."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1569","DOI":"10.1111\/jth.14846","article-title":"Lupus anticoagulant detection in anticoagulated patients. Guidance from the Scientific and Standardization Committee for lupus anticoagulant\/antiphospholipid antibodies of the International Society on Thrombosis and Haemostasis","volume":"18","author":"Tripodi","year":"2020","journal-title":"J. Thromb. Haemost."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"102385","DOI":"10.1016\/j.rpth.2024.102385","article-title":"Testing for the lupus anticoagulant: The good, the bad, and the ugly","volume":"8","author":"Favaloro","year":"2024","journal-title":"Res. Pract. Thromb. Haemost."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Favaloro, E.J., Pasalic, L., and Lippi, G. (2025). Oral Anticoagulation Therapy: An Update on Usage and Costs in the Endemic COVID-19 Era. J. Clin. Med., 14.","DOI":"10.3390\/jcm14082591"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.thromres.2019.05.013","article-title":"Neutralising rivaroxaban induced interference in laboratory testing for lupus anticoagulant (LA): A comparative study using DOAC Stop and andexanet alfa","volume":"180","author":"Favaloro","year":"2019","journal-title":"Thromb. Res."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Exner, T., Dangol, M., and Favaloro, E.J. (2024). Simplified method for removing DOAC interference in mechanical coagulation test systems\u2014A proof of concept. J. Clin. Med., 13.","DOI":"10.3390\/jcm13041042"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1111\/ijlh.13818","article-title":"A multi-laboratory assessment of lupus anticoagulant assays performed on the ACL TOP 50 family for harmonized testing in a large laboratory network","volume":"44","author":"Favaloro","year":"2022","journal-title":"Int. J. Lab. Hematol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1111\/bjh.16431","article-title":"What is the appropriate anticoagulation strategy for thrombotic antiphospholipid syndrome?","volume":"189","author":"Arachchillage","year":"2020","journal-title":"Br. J. Haematol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1055\/s-0043-1761487","article-title":"Evolution of hemostasis testing: A personal reflection covering over 40 years of history","volume":"50","author":"Favaloro","year":"2024","journal-title":"Semin. Thromb. Hemost."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"992","DOI":"10.1002\/ajh.25836","article-title":"Alternative assays to dRVVT and aPTT for lupus anticoagulant detection","volume":"95","author":"Moore","year":"2020","journal-title":"Am. J. Hematol."},{"key":"ref_46","first-page":"17006","article-title":"Direct oral anticoagulants versus vitamin-K antagonists in patients with single or double-positive antiphospholipid syndrome: A systematic review and meta-analysis","volume":"135","author":"Nicoletto","year":"2025","journal-title":"Pol. Arch. Intern. Med."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"102724","DOI":"10.1016\/j.rpth.2025.102724","article-title":"Unresolved issues in the diagnosis and management of thrombotic antiphospholipid syndrome","volume":"9","author":"Arachchillage","year":"2025","journal-title":"Res. Pract. Thromb. Haemost."},{"key":"ref_48","unstructured":"Clinical and Laboratory Standards Institute (CLSI) (2014). Laboratory Testing for the Lupus Anticoagulant, CLSI. CLSI document H60-A."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1111\/bjh.19635","article-title":"Guidelines on the investigation and management of antiphospholipid syndrome","volume":"205","author":"Arachchillage","year":"2024","journal-title":"Br. J. Haematol."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Martini, T., Santoro, R.C., Banov, L., Ierardi, A., Leotta, M., Strangio, A., Svahn, J., and Molinari, A.C. (2024). Prolongated Activated Partial Thromboplastin Time (aPTT) in Pediatric Patients before Surgery-Crying Wolf: Lupus (Anticoagulant) Does Not Always Threaten Children. J. Clin. Med., 13.","DOI":"10.3390\/jcm13051510"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1055\/s-0042-1744363","article-title":"Testing for Lupus Anticoagulant","volume":"48","author":"Moore","year":"2022","journal-title":"Semin. Thromb. Hemost."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Marco-Rico, A. (2025). Update on the Laboratory Diagnosis of Lupus Anticoagulant: Current Challenges and Clinical Involvement. J. Clin. Med., 14.","DOI":"10.3390\/jcm14082791"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Molinari, A.C., Martini, T., Banov, L., Ierardi, A., Leotta, M., Strangio, A., and Santoro, R.C. (2023). Lupus Anticoagulant Detection under the Magnifying Glass. J. Clin. Med., 12.","DOI":"10.3390\/jcm12206654"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Vandevelde, A., and Devreese, K.M.J. (2022). Laboratory Diagnosis of Antiphospholipid Syndrome: Insights and Hindrances. J. Clin. Med., 11.","DOI":"10.3390\/jcm11082164"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Tohidi-Esfahani, I., Mittal, P., Isenberg, D., Cohen, H., and Efthymiou, M. (2024). Platelets and Thrombotic Antiphospholipid Syndrome. J. Clin. Med., 13.","DOI":"10.20944\/preprints202401.0466.v1"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1007\/978-1-0716-3175-1_19","article-title":"Antiphospholipid Antibody Testing for Anti-cardiolipin and Anti-\u03b22 Glycoprotein I Antibodies Using Chemiluminescence-Based Panels","volume":"2663","author":"Favaloro","year":"2023","journal-title":"Methods Mol. Biol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"102641","DOI":"10.1016\/j.autrev.2020.102641","article-title":"Evaluation of novel assays for the detection of autoantibodies in antiphospholipid syndrome","volume":"19","author":"Sciascia","year":"2020","journal-title":"Autoimmun. Rev."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1111\/jth.15126","article-title":"New anticoagulants: Moving beyond the direct oral anticoagulants","volume":"19","author":"Fredenburgh","year":"2021","journal-title":"J. Thromb. Haemost."}],"container-title":["Journal of Clinical Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2077-0383\/14\/14\/4812\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:06:12Z","timestamp":1760033172000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2077-0383\/14\/14\/4812"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,8]]},"references-count":58,"journal-issue":{"issue":"14","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["jcm14144812"],"URL":"https:\/\/doi.org\/10.3390\/jcm14144812","relation":{},"ISSN":["2077-0383"],"issn-type":[{"value":"2077-0383","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,8]]}}}