{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:40:00Z","timestamp":1760060400319,"version":"build-2065373602"},"reference-count":43,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T00:00:00Z","timestamp":1756166400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Institute of Mother and Child"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Background\/Objectives: The term \u2018cystic fibrosis transmembrane conductance regulator-related metabolic syndrome\/cystic fibrosis screen positive, inconclusive diagnosis (CRMS\/CFSPID)\u2019 refers to patients with positive screening tests but without a final diagnosis of Cystic Fibrosis (CF). Intestinal Current Measurement (ICM) is a novel diagnostic technique that may document the abnormal function of the cystic fibrosis transmembrane conductance regulator. Our study aims to compare the cumulative chloride secretory response in the ICM study in the Polish population of CF patients, CRMS\/CFSPID, and in a control group. Methods: Forceps rectal biopsies were taken from 40 patients (CF; n = 17 mean age 9.10 \u00b1 4.18 (0.7\u201317.20); CRMS\/CFSPID: n = 16, mean age 6.66 \u00b1 4.83 (0.6\u201318.0); healthy controls (HC): n = 7, mean age 23.7 \u00b1 9.5 (7.8\u201334.6). ICM tests were performed in the Ussing Chamber according to standard protocol version 2.7 of the European Cystic Fibrosis Society Diagnostic Network Working Group. Delta short circuit-current (\u0394Isc) was measured after carbachol (\u0394Isccarbachol), 3-isobutyl-1-methylxanthine with forskolin (\u0394IscIBMX\/forskolin), and histamine (\u0394ischistamine) stimulation. Cumulative secretion was calculated for each study group. Results: We obtained statistically significant differences in cumulative chloride secretory response between CF and CRMS\/CFSPID (CF \u0394Isccarbachol+IBMX\/forskolin+histamine 15.32 \u00b1 15.47 \u00b5A\/cm2 vs. CRMS\/CFSPID \u0394Isccarbachol+IBMX\/forskolin+histamine 86.84 \u00b1 37.84 \u00b5A\/cm2; p &lt; 0.001), and between CF and healthy controls (CF \u0394Isccarbachol+IBMX\/forskolin+histamine 15.32 \u00b1 15.47 \u00b5A\/cm2 vs. HC \u0394Isccarbachol+IBMX\/forskolin+histamine 80.16 \u00b1 48.54 \u00b5A\/cm2; p = 0.005). No differences in cumulative chloride secretion were observed between the CRMS\/CFSPID and HC groups. Conclusions: The conducted study suggests that ICM may offer diagnostic value, especially in cases where sweat test results are equivocal.<\/jats:p>","DOI":"10.3390\/jcm14176020","type":"journal-article","created":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T10:35:17Z","timestamp":1756204517000},"page":"6020","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Pilot Evaluation of Intestinal Current Measurement in Cystic Fibrosis and CRMS\/CFSPID Patients in Poland"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1678-3316","authenticated-orcid":false,"given":"Magdalena","family":"Postek","sequence":"first","affiliation":[{"name":"Cystic Fibrosis Department, Institute of Mother and Child, 01-211 Warsaw, Poland"},{"name":"Cystic Fibrosis Centre, Pediatric Hospital, 05-092 Dziekanow Lesny, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katarzyna","family":"Zybert","sequence":"additional","affiliation":[{"name":"Cystic Fibrosis Department, Institute of Mother and Child, 01-211 Warsaw, Poland"},{"name":"Cystic Fibrosis Centre, Pediatric Hospital, 05-092 Dziekanow Lesny, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8702-3808","authenticated-orcid":false,"given":"Lukasz","family":"Wozniacki","sequence":"additional","affiliation":[{"name":"Cystic Fibrosis Department, Institute of Mother and Child, 01-211 Warsaw, Poland"},{"name":"Cystic Fibrosis Centre, Pediatric Hospital, 05-092 Dziekanow Lesny, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marek","family":"Woynarowski","sequence":"additional","affiliation":[{"name":"Cystic Fibrosis Centre, Pediatric Hospital, 05-092 Dziekanow Lesny, Poland"},{"name":"Department of Paediatrics, Collegium Medicum, Jan Kochanowski University, 25-317 Kielce, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorota","family":"Sands","sequence":"additional","affiliation":[{"name":"Cystic Fibrosis Department, Institute of Mother and Child, 01-211 Warsaw, Poland"},{"name":"Cystic Fibrosis Centre, Pediatric Hospital, 05-092 Dziekanow Lesny, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.jcf.2018.02.006","article-title":"ECFS best practice guidelines: The 2018 revision","volume":"17","author":"Castellani","year":"2018","journal-title":"J. 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