{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T11:29:49Z","timestamp":1786620589353,"version":"build-2736575974"},"reference-count":20,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T00:00:00Z","timestamp":1756339200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Objectives: This retrospective single-center cohort study aims to evaluate the impact of dual-trigger therapy (recombinant hCG [rhCG] combined with GnRH agonist) compared to rhCG alone on ART outcomes in women undergoing GnRH antagonist protocols. Methods: Data from 1291 IVF cycles performed between 2016 and 2022 were analyzed. After propensity score matching (PSM) to adjust for confounders, 395 cycles in each group were compared. Primary outcomes included the total number of oocytes retrieved, while secondary outcomes assessed mature oocytes, fertilization rates, and embryo numbers. Results: Dual-trigger therapy yielded significantly more total oocytes (7.50 \u00b1 5.23 vs. 6.12 \u00b1 4.23, p &lt; 0.001) and mature oocytes (5.67 \u00b1 3.87 vs. 5.01 \u00b1 3.13, p = 0.047) compared to rhCG alone. Cycles with no oocytes were fewer in the dual-trigger group (1.3% vs. 3.8%, p = 0.015). Total embryos were also higher with dual trigger therapy (2.43 \u00b1 1.90 vs. 2.00 \u00b1 1.93, p = 0.001). In intracytoplasmic sperm injection (ICSI) cycles, the fertilization rate significantly improved with dual trigger (64.93 \u00b1 33.50% vs. 52.22 \u00b1 34.12%, p = 0.003). No significant differences were noted in fertilization rates for standard IVF (55.14 \u00b1 30.72% vs. 52.29 \u00b1 32.11%, p = 0.18) or maturation rates (72.52 \u00b1 26.91% vs. 71.53 \u00b1 24.75%, p = 0.37). Conclusions: These findings demonstrate that dual-trigger therapy improves ART outcomes by increasing oocyte and embryo yields.<\/jats:p>","DOI":"10.3390\/jcm14176096","type":"journal-article","created":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T15:03:14Z","timestamp":1756393394000},"page":"6096","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effect of Ovarian Stimulation and Trigger Protocols on Oocyte and Embryo Numbers\u2014Real World Experience"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4611-935X","authenticated-orcid":false,"given":"Shmuel","family":"Somer","sequence":"first","affiliation":[{"name":"Sheba Medical Center, Tel Hashomer, Ramat Gan 52621, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Nothman","sequence":"additional","affiliation":[{"name":"Genea Fertility, Sydney, NSW 2000, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1480-9283","authenticated-orcid":false,"given":"Shira","family":"Baram","sequence":"additional","affiliation":[{"name":"Fertility and In-Vitro Fertilization Unit, Department of Obstetrics and Gynecology, Emek Medical Center, Afula 1834111, Israel"},{"name":"Rappaport Faculty of Medicine, Technion Institute of Technology, Haifa 3525433, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4525-4683","authenticated-orcid":false,"given":"Ido","family":"Izhaki","sequence":"additional","affiliation":[{"name":"Department of Evolutionary and Environmental Biology, Faculty of Natural Sciences, University of Haifa, Haifa 3103301, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nitzan Dana","family":"Sela","sequence":"additional","affiliation":[{"name":"Fertility and In-Vitro Fertilization Unit, Department of Obstetrics and Gynecology, Emek Medical Center, Afula 1834111, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4871-9829","authenticated-orcid":false,"given":"Ronit","family":"Beck-Fruchter","sequence":"additional","affiliation":[{"name":"Fertility and In-Vitro Fertilization Unit, Department of Obstetrics and Gynecology, Emek Medical Center, Afula 1834111, Israel"},{"name":"Rappaport Faculty of Medicine, Technion Institute of Technology, Haifa 3525433, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1111\/j.1471-0528.1980.tb04610.x","article-title":"Establishing full-term human pregnancies using cleaving embryos grown in vitro","volume":"87","author":"Edwards","year":"1980","journal-title":"Br. 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