{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T22:40:38Z","timestamp":1782254438503,"version":"3.54.5"},"reference-count":30,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T00:00:00Z","timestamp":1757635200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Public Service of Wallonia","award":["7819"],"award-info":[{"award-number":["7819"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Background: NVD003 is an autologous, adipose tissue-derived stem cell-based tissue-engineered bone graft substitute with pro-osteogenic, anti-resorptive, and pro-angiogenic properties. Here, we describe highlights from the NVD003 preclinical development program as well as early clinical experience. Methods: NVD003 is produced in a Good Manufacturing Practice-controlled process from adipose stem cells collected during a minimally invasive liposuction procedure. The final implant is a ready-to-use moldable putty with fixed mineral content and predefined physiologic ranges of osteogenic cells and bioactive growth factors. Preclinical pharmacology studies were conducted in nude rats using a paravertebral implantation model, and subsequently, in a femoral critical-sized bone defect (CSBD) model. In a first-in-human Phase 1b\/2a study, NVD003 was used for fracture osteosynthesis with classical fixation material in nine adults with recalcitrant lower limb non-union. NVD003 was also used at the discretion of treating physicians in four pediatric patients surgically treated for congenital pseudarthrosis of the tibia (CPT) with the Masquelet technique. Efficacy was evaluated as clinical healing and in terms of bone formation, bone union, and bone remodeling on radiographs and computed tomography using the extended Lane and Sandhu Scale. Results: Preclinical studies indicated that NVD003 requires cellularity for its bioactivity and moreover facilitates bone union when used as a graft material in femoral CSBD. In the clinical study, nine adult participants were successfully grafted with NVD003 and completed study follow-up to 24 months, with extended safety follow-up to 5 years ongoing. No adverse events were considered related to NVD003. Maximal bone formation occurred between 3 and 12 months post-implantation; the mean time to clinical healing was 6 months and the mean time to radiological union was 17 months. Ultimately, 89% (8\/9) of patients achieved bone union without refracture. All four pediatric patients with CPT also achieved lasting bone union following grafting with NVD003. No safety signals were observed over a mean follow-up of 62.1 months. Conclusions: NVD003 represents a safe, autologous bone graft substitute product without side effects of heterotopic ossification or bone resorption. NVD003 facilitated bone union in adult and pediatric patients even under severe pathophysiological conditions.<\/jats:p>","DOI":"10.3390\/jcm14186436","type":"journal-article","created":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T12:08:16Z","timestamp":1757678896000},"page":"6436","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["An Autologous Human Adipose Stem Cell-Derived 3D Osteogenic Implant for Bone Grafting: From Development to First-in-Human Experience"],"prefix":"10.3390","volume":"14","author":[{"given":"Torsten","family":"Gerich","sequence":"first","affiliation":[{"name":"Centre Hospitalier de Luxembourg, 4 Rue Ernest Barbl\u00e9, L-1210 Luxembourg, Luxembourg"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre-Louis","family":"Docquier","sequence":"additional","affiliation":[{"name":"Department of Orthopedic and Trauma Surgery, Cliniques Universitaires Saint-Luc, Universit\u00e9 Catholique de Louvain\u2014UCLouvain, 1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3472-2469","authenticated-orcid":false,"given":"John A.","family":"Carrino","sequence":"additional","affiliation":[{"name":"Weill Cornell Medicine, Hospital for Special Surgery, New York, NY 10021, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8774-6563","authenticated-orcid":false,"given":"Mikael","family":"Boesen","sequence":"additional","affiliation":[{"name":"Department of Radiology, Frederiksberg Hospital, Nordrefasanvej 57, 2000 Frederiksberg, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadine","family":"Schmid","sequence":"additional","affiliation":[{"name":"Centre Hospitalier de Luxembourg, 4 Rue Ernest Barbl\u00e9, L-1210 Luxembourg, Luxembourg"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ginny","family":"Hsu","sequence":"additional","affiliation":[{"name":"Pathology Department, Johns Hopkins University, Baltimore, MD 21218, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji-Hye","family":"Yea","sequence":"additional","affiliation":[{"name":"Pathology Department, Johns Hopkins University, Baltimore, MD 21218, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron","family":"James","sequence":"additional","affiliation":[{"name":"Pathology Department, Johns Hopkins University, Baltimore, MD 21218, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judy","family":"Ashworth","sequence":"additional","affiliation":[{"name":"Novadip Biosciences, 1435 Mont-Saint-Guibert, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hara","family":"Episkopou","sequence":"additional","affiliation":[{"name":"Novadip Biosciences, 1435 Mont-Saint-Guibert, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2897-0362","authenticated-orcid":false,"given":"Denis","family":"Dufrane","sequence":"additional","affiliation":[{"name":"Novadip Biosciences, 1435 Mont-Saint-Guibert, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1302\/2046-3758.116.BJR-2022-0186","article-title":"The reconstruction of critical bone loss: The holy grail of orthopaedics","volume":"11","author":"Tsang","year":"2022","journal-title":"Bone Joint Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"S7","DOI":"10.1097\/BOT.0000000000001115","article-title":"Critical-size bone defects: Is there a consensus for diagnosis and treatment?","volume":"32","author":"Nauth","year":"2018","journal-title":"J. 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