{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:54:46Z","timestamp":1759334086957,"version":"build-2065373602"},"reference-count":36,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T00:00:00Z","timestamp":1759104000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["RS-2023-00209015"],"award-info":[{"award-number":["RS-2023-00209015"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007783","name":"research fund of Hanyang University","doi-asserted-by":"publisher","award":["HY-202300000001144"],"award-info":[{"award-number":["HY-202300000001144"]}],"id":[{"id":"10.13039\/501100007783","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.mdpi.com"],"crossmark-restriction":true},"short-container-title":["JCM"],"abstract":"<jats:p>Background\/Objectives: To investigate microRNA (miRNA) expression profiles in the anterior lens capsules of patients with glucocorticoid-induced cataracts (GIC) and to identify miRNAs potentially associated with glucocorticoid (GC) exposure and posterior subcapsular cataract (PSC) formation. Methods: A total of 33 participants were divided into four groups based on their GC usage history and cataract type: GIC (n = 10), age-related PSC (n = 6), GC-treated age-related cataract (ARC) (n = 7), and normal control (n = 10). Anterior lens capsule samples were obtained during cataract surgery and total RNA was extracted for quantitative real-time polymerase chain reaction (qRT-PCR). The expression levels of 12 selected miRNAs were quantified using a customized miScript miRNA PCR array. Results: Among the twelve miRNAs analyzed, seven (let-7a-5p, let-7d-5p, miR-15a-5p, miR-16-5p, miR-23b-3p, miR-26a-5p, and miR-125a-5p) were significantly differentially expressed among the groups (p &lt; 0.05). In the GIC group, let-7a-5p, miR-23b-3p, miR-26a-5p, and miR-125a-5p were significantly upregulated, whereas let-7d-5p, miR-15a-5p and miR-16-5p were significantly downregulated compared to that in the normal control group. No significant differences in miRNA expression were observed between the GIC and age-related PSC groups or between the GIC and GC-treated ARC groups. Conclusions: This study demonstrates distinct miRNA expression patterns in the anterior lens capsules of patients with GIC. Altered expression of specific miRNAs may be linked to the pathogenesis of GC-induced PSC formation. These findings provide a molecular basis for further investigation into the regulatory roles of miRNAs in GC-associated cataracts.<\/jats:p>","DOI":"10.3390\/jcm14196909","type":"journal-article","created":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T09:12:23Z","timestamp":1759223543000},"page":"6909","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Differential MicroRNA Expression in the Anterior Lens Capsule of Patients with Glucocorticoid-Induced Cataracts: A Preliminary Study"],"prefix":"10.3390","volume":"14","author":[{"given":"Yeji","family":"Yeon","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, Hanyang University College of Medicine, Seoul 04763, Republic of Korea"},{"name":"Hanyang Vision Research Center, Hanyang University, Seoul 04763, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soo Rack","family":"Ryu","sequence":"additional","affiliation":[{"name":"Biostatistical Consulting and Research Laboratory, Medical Research Collaborating Center, Hanyang University, Seoul 04763, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min-Ji","family":"Cha","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Hanyang University College of Medicine, Seoul 04763, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3506-7139","authenticated-orcid":false,"given":"Won June","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Hanyang University College of Medicine, Seoul 04763, Republic of Korea"},{"name":"Hanyang Vision Research Center, Hanyang University, Seoul 04763, Republic of Korea"},{"name":"Department of Ophthalmology, Hanyang University Seoul Hospital, Seoul 04763, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han Woong","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Hanyang University College of Medicine, Seoul 04763, Republic of Korea"},{"name":"Hanyang Vision Research Center, Hanyang University, Seoul 04763, Republic of Korea"},{"name":"Department of Ophthalmology, Hanyang University Seoul Hospital, Seoul 04763, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7454-1350","authenticated-orcid":false,"given":"Ji Hong","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Hanyang University College of Medicine, Seoul 04763, Republic of Korea"},{"name":"Hanyang Vision Research Center, Hanyang University, Seoul 04763, Republic of Korea"},{"name":"Department of Ophthalmology, Hanyang University Seoul Hospital, Seoul 04763, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4375-1472","authenticated-orcid":false,"given":"Yu Jeong","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Hanyang University College of Medicine, Seoul 04763, Republic of Korea"},{"name":"Hanyang Vision Research Center, Hanyang University, Seoul 04763, Republic of Korea"},{"name":"Department of Ophthalmology, Hanyang University Seoul Hospital, Seoul 04763, Republic of Korea"},{"name":"Hanyang Institute of Bioscience and Biotechnology, Hanyang University, Seoul 04763, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e339","DOI":"10.1016\/S2214-109X(13)70113-X","article-title":"Causes of vision loss worldwide, 1990\u20132010: A systematic analysis","volume":"1","author":"Bourne","year":"2013","journal-title":"Lancet Glob. 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