{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T20:22:06Z","timestamp":1789762926868,"version":"4.0.1"},"reference-count":58,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T00:00:00Z","timestamp":1759881600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Background\/Objectives: The objective of this in vitro study was to compare and evaluate the in vitro antimicrobial effectiveness of Endosal, Octenisolv, and Endoxal against intracanal Enterococcus faecalis. Methods: The study sample consisted of 84 extracted single-rooted human teeth, which were divided into seven groups (12 roots in each group): Group 1\u2014Endoxal, Group 2\u2014Octenisolv, Group 3\u2014Endosal, Group 4\u201415% ethylenediaminetetraacetic acid (EDTA), Group 5\u20142% sodium hypochlorite (NaOCl), Group 6\u20140.9% sterile saline solution (NaCl), and one positive control group where no irrigant was used. The roots were sterilized within an autoclave for 30 min at 121 \u00b0C and then contaminated with E. faecalis bacteria, after instrumentation and removing the smear layer from canals. The root canals were irrigated using a side-vented needle, and then ISO size 40 H-file was used to obtain fine dentine chips. Aliquots taken from the canals were plated on blood agar broth and the plates were incubated for 36 h. Results: In this study, significant differences were observed between the antimicrobial activity of Endoxal, Octenisolv, Endosal, 2% NaOCl, and sterile saline solution. Conclusions: The compound irrigants Endosal, Endoxal, and a novel irrigant containing disodium edetate and octenidine, which were evaluated in this study, exhibited relatively good antimicrobial properties against Enterococcus faecalis. The use of Endosal, Octenisolv or Endoxal appears promising, yet their clinical efficacy remains to be confirmed through further studies.<\/jats:p>","DOI":"10.3390\/jcm14197100","type":"journal-article","created":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T07:56:29Z","timestamp":1759996589000},"page":"7100","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["In Vitro Evaluation of Antimicrobial Effects of Endodontic Irrigants Containing Disodium Edetate and Chlorhexidine Gluconate, Octenidine Dihydrochloride, and Benzalkonium Bromide Against Intracanal Enterococcus faecalis"],"prefix":"10.3390","volume":"14","author":[{"given":"Anna","family":"Siemi\u0144ska","sequence":"first","affiliation":[{"name":"Private Dental Practice, Klemensa Janickiego 16A, 71-270 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Kot","sequence":"additional","affiliation":[{"name":"Department of Preclinical Conservative Dentistry and Preclinical Endodontics, Pomeranian Medical University in Szczecin, Al. Powst. Wlkp. 72, 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ewa","family":"Marek","sequence":"additional","affiliation":[{"name":"Department of Preclinical Conservative Dentistry and Preclinical Endodontics, Pomeranian Medical University in Szczecin, Al. Powst. Wlkp. 72, 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6266-9792","authenticated-orcid":false,"given":"Agnieszka","family":"Chamarczuk","sequence":"additional","affiliation":[{"name":"Department of Preclinical Conservative Dentistry and Preclinical Endodontics, Pomeranian Medical University in Szczecin, Al. Powst. Wlkp. 72, 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Kacza\u0142a","sequence":"additional","affiliation":[{"name":"Private Dental Practice, Energetyk\u00f3w 14C, 74-100 Gryfino, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Ras\u0142awska-Socha","sequence":"additional","affiliation":[{"name":"Department of Preclinical Conservative Dentistry and Preclinical Endodontics, Pomeranian Medical University in Szczecin, Al. Powst. Wlkp. 72, 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7612-3148","authenticated-orcid":false,"given":"Laurentia","family":"Schuster","sequence":"additional","affiliation":[{"name":"Department of Periodontology and Operative Dentistry, University of M\u00fcnster, Waldeyerstra\u00dfe 30, 48149 M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1276-0632","authenticated-orcid":false,"given":"Till","family":"Dammaschke","sequence":"additional","affiliation":[{"name":"Department of Periodontology and Operative Dentistry, University of M\u00fcnster, Waldeyerstra\u00dfe 30, 48149 M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1103-1297","authenticated-orcid":false,"given":"Liliana","family":"Szyszka-Sommerfeld","sequence":"additional","affiliation":[{"name":"Laboratory for Propaedeutics of Orthodontics and Facial Congenital Defects, Chair of Maxillofacial Orthopaedics and Orthodontics, Pomeranian Medical University in Szczecin, Al. Powst. Wlkp. 72, 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2567-3362","authenticated-orcid":false,"given":"Mariusz","family":"Lipski","sequence":"additional","affiliation":[{"name":"Department of Preclinical Conservative Dentistry and Preclinical Endodontics, Pomeranian Medical University in Szczecin, Al. Powst. Wlkp. 72, 70-111 Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,10,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Fiegler-Rudol, J., Skaba, D., and Wiench, R. (2025). Antimicrobial Efficacy of Nd:YAG Laser in Polymicrobial Root Canal Infections: A Systematic Review of In Vitro Studies. Int. J. Mol. 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