{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T02:00:08Z","timestamp":1787277608611,"version":"build-2736575974"},"reference-count":25,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T00:00:00Z","timestamp":1769212800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Medical & Biological Laboratories Co., Ltd.","award":["IIS"],"award-info":[{"award-number":["IIS"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Background\/Objectives: Differential diagnosis between Crohn\u2019s disease (CD) and ulcerative colitis (UC) can be sometimes difficult resulting in the diagnosis of unspecified inflammatory bowel diseases (IBD-U). Data suggest that IgG antibodies against integrin \u03b1v\u03b26 (V6 Ab) help to identify UC patients. Recent studies suggest that measuring V6 Ab serum levels may be valuable for differential diagnostic purposes. The primary objective of the study was to assess the sensitivity and specificity of V6 Ab serum-level measurement in our IBD population to differentiate between colonic\/ileocolonic CD and UC with an established diagnosis. Furthermore, we assessed the correlation between disease characteristics, activity and V6 Ab serum levels in UC patients. Methods: Consecutive IBD patients with an established diagnosis undergoing control colonoscopy in a tertiary IBD center were included. Baseline demographic data, current treatment, disease extent, clinical, biomarker, endoscopic and histologic disease activity were collected. V6 Ab serum levels were measured with the Anti-Integrin \u03b1v\u03b26 ELISA Kit (RUO). Patients\u2019 written informed consent was obtained. Results: A total of 40 IBD patients, including 10 CD and 30 UC patients (15 with clinical activity and 15 in clinical remission) were enrolled. V6 Ab serum levels were significantly higher in UC patients compared to CD (p = 0.039). ROC analysis found 1.33 U\/mL to be the best cut-off level (p = 0.04; AUC: 0.71) with 100% sensitivity and 50% specificity and a positive predictive value of 85.7% and a negative predictive value of 100% to differentiate between UC and CD. No significant correlation was found between V6 Ab serum levels and CRP (p = 0.057), fecal calprotectin (p = 0.77), endoscopic activity (p = 0.624) or disease extent (p = 0.624) in UC patients. Conclusions: Our study supports the value of V6 Ab serum level measurement as a differential diagnostic tool in IBD patients; however, the optimal cut-off value is yet to be determined. Our data do not support its role in disease activity monitoring.<\/jats:p>","DOI":"10.3390\/jcm15030948","type":"journal-article","created":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T09:21:40Z","timestamp":1769505700000},"page":"948","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Clinical Value of Anti-Integrin \u03b1v\u03b26 Antibody Serum-Level Measurement in Inflammatory Bowel Diseases"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-0900-1966","authenticated-orcid":false,"given":"Dorottya","family":"Angyal","sequence":"first","affiliation":[{"name":"Department of Internal Medicine and Oncology, Semmelweis University, 1083 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4623-4024","authenticated-orcid":false,"given":"Fruzsina","family":"Balogh","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Oncology, Semmelweis University, 1083 Budapest, Hungary"},{"name":"Division of Gastroenterology, Central Hospital of Northern Pest, Military Hospital, 1134 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8819-6460","authenticated-orcid":false,"given":"Lorant","family":"Gonczi","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Oncology, Semmelweis University, 1083 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6087-7398","authenticated-orcid":false,"given":"Livia","family":"Lontai","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Oncology, Semmelweis University, 1083 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7510-8387","authenticated-orcid":false,"given":"Janos P.","family":"Kosa","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Oncology, Semmelweis University, 1083 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1959-4473","authenticated-orcid":false,"given":"Nora","family":"Garam","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Oncology, Semmelweis University, 1083 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3948-6488","authenticated-orcid":false,"given":"Peter L.","family":"Lakatos","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Oncology, Semmelweis University, 1083 Budapest, Hungary"},{"name":"Division of Gastroenterology, McGill University, Montreal, QC H4A 3J1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9865-2642","authenticated-orcid":false,"given":"Akos","family":"Ilias","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Oncology, Semmelweis University, 1083 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1340","DOI":"10.1002\/ibd.21903","article-title":"Serological antibodies in inflammatory bowel disease: A systematic review","volume":"18","author":"Prideaux","year":"2012","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1111\/j.1572-0241.2001.03613.x","article-title":"Diagnostic value of anti-Saccharomyces cerevisiae and antineutrophil cytoplasmic autoantibodies in inflammatory bowel disease","volume":"96","author":"Peeters","year":"2001","journal-title":"Am. J. Gastroenterol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1159\/000442934","article-title":"ASCA, ANCA, ALCA and Many More: Are They Useful in the Diagnosis of Inflammatory Bowel Disease?","volume":"34","author":"Zhou","year":"2016","journal-title":"Dig. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1111\/j.1572-0241.2007.01652.x","article-title":"New serological markers for inflammatory bowel disease are associated with earlier age at onset, complicated disease behavior, risk for surgery, and NOD2\/CARD15 genotype in a Hungarian IBD cohort","volume":"103","author":"Papp","year":"2008","journal-title":"Am. J. Gastroenterol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1093\/ecco-jcc\/jjv127","article-title":"Risk Matrix for Prediction of Disease Progression in a Referral Cohort of Patients with Crohn\u2019s Disease","volume":"9","author":"Lakatos","year":"2015","journal-title":"J. Crohns Colitis"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"7167","DOI":"10.2147\/JIR.S518155","article-title":"N4BP3 Activates TLR4-NF-\u03baB Pathway in Inflammatory Bowel Disease by Promoting K48-Linked I\u03baB\u03b1 Ubiquitination","volume":"18","author":"Jiang","year":"2025","journal-title":"J. Inflamm. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1016\/S0092-8674(02)00971-6","article-title":"Integrins: Bidirectional, allosteric signaling machines","volume":"110","author":"Hynes","year":"2002","journal-title":"Cell"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2383","DOI":"10.1053\/j.gastro.2021.02.019","article-title":"Identification of an Anti-Integrin \u03b1v\u03b26 Autoantibody in Patients With Ulcerative Colitis","volume":"160","author":"Kuwada","year":"2021","journal-title":"Gastroenterology"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2241","DOI":"10.1242\/jcs.108.6.2241","article-title":"Expression of the beta 6 integrin subunit in development, neoplasia and tissue repair suggests a role in epithelial remodeling","volume":"108","author":"Breuss","year":"1995","journal-title":"J. Cell Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1002\/cbin.10258","article-title":"Alphavbeta6 is required in maintaining the intestinal epithelial barrier function","volume":"38","author":"Yu","year":"2014","journal-title":"Cell Biol. Int."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.dld.2023.06.024","article-title":"Diagnostic value of anti-integrin \u03b1v\u03b26 antibodies in ulcerative colitis","volume":"56","author":"Marafini","year":"2023","journal-title":"Dig. Liver Dis."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1080\/00365520600554527","article-title":"Change of diagnosis during the first five years after onset of inflammatory bowel disease: Results of a prospective follow-up study (the IBSEN Study)","volume":"41","author":"Henriksen","year":"2006","journal-title":"Scand. J. Gastroenterol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1111\/jgh.14563","article-title":"Disease course of inflammatory bowel disease unclassified in a European population-based inception cohort: An Epi-IBD study","volume":"34","author":"Burisch","year":"2019","journal-title":"J. Gastroenterol. Hepatol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"i995","DOI":"10.1093\/ecco-jcc\/jjac190.1006","article-title":"P876 Disease course of Inflammatory Bowel Disease Unclassified in adult patients: A population-based study (1988\u20132014)","volume":"17","author":"Jougon","year":"2023","journal-title":"J. Crohn\u2019s Colitis"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"109800","DOI":"10.3748\/wjg.v31.i33.109800","article-title":"Incidence, disease course, therapeutic strategies and outcomes of inflammatory bowel disease-unclassified patients in Western Hungary: A population-based cohort","volume":"31","author":"Balogh","year":"2025","journal-title":"World J. Gastroenterol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1242","DOI":"10.1053\/gast.2002.32980","article-title":"The value of serologic markers in indeterminate colitis: A prospective follow-up study","volume":"122","author":"Joossens","year":"2002","journal-title":"Gastroenterology"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Rydell, N., Ekoff, H., Hellstr\u00f6m, P.M., and Mov\u00e9rare, R. (2022). Measurement of Serum IgG Anti-Integrin \u03b1v\u03b26 Autoantibodies Is a Promising Tool in the Diagnosis of Ulcerative Colitis. J. Clin. Med., 11.","DOI":"10.3390\/jcm11071881"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1053\/j.gastro.2022.12.042","article-title":"Anti-Integrin \u03b1v\u03b26 Autoantibodies Are a Novel Biomarker That Antedate Ulcerative Colitis","volume":"164","author":"Livanos","year":"2023","journal-title":"Gastroenterology"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Bez, P., Scapolan, M., Ribaldone, D.G., Caviglia, G.P., Zago, S., Trucco, C., Frara, S., Caruso, A., Ascolani, M., and Campigotto, M. (2025). Antibodies against integrin \u03b1v\u03b26 have high diagnostic accuracy for ulcerative colitis. Front. Immunol., 16.","DOI":"10.3389\/fimmu.2025.1641329"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"jjaf062","DOI":"10.1093\/ecco-jcc\/jjaf062","article-title":"Anti-integrin \u03b1v\u03b26 IgG antibody as a diagnostic and prognostic marker in ulcerative colitis: A cross-sectional and longitudinal study defining a specific disease phenotype","volume":"19","author":"Pertsinidou","year":"2025","journal-title":"J. Crohns Colitis"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1007\/s00535-024-02176-x","article-title":"Anti-integrin \u03b1v\u03b26 antibody as a biomarker for diagnosing ulcerative colitis: A nationwide multicenter validation study","volume":"60","author":"Okabe","year":"2025","journal-title":"J. Gastroenterol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"100582","DOI":"10.1016\/j.gastha.2024.10.022","article-title":"Anti-Integrin \u03b1v\u03b26 Antibody Titer as a Predictive Biomarker of Future Treatment Escalation in Patients With Ulcerative Colitis","volume":"4","author":"Yamamoto","year":"2024","journal-title":"Gastro Hep Adv."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Yanagida, T., Nishida, Y., Kobayashi, Y., Nakata, R., Hosomi, S., Maruyama, H., Ominami, M., Nadatani, Y., Fukunaga, S., and Otani, K. (Intest. Res., 2025). Anti-integrin \u03b1v\u03b26 autoantibody in patients with ulcerative colitis after proctocolectomy: A cross-sectional study in Japan, Intest. Res., ahead of print.","DOI":"10.5217\/ir.2024.00170"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1093\/ibd\/izae263","article-title":"Anti-integrin \u03b1v\u03b26 Antibodies Predict Pouchitis in Patients With Ulcerative Colitis After Restorative Proctocolectomy With Ileal Pouch-Anal Anastomosis","volume":"31","author":"Nakanishi","year":"2025","journal-title":"Inflamm. Bowel Dis."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1108","DOI":"10.1007\/s00535-025-02263-7","article-title":"Autoantibodies against endothelial protein C receptor and integrin \u03b1v\u03b26 predict the development of ulcerative colitis","volume":"60","author":"Sawahashi","year":"2025","journal-title":"J. Gastroenterol."}],"container-title":["Journal of Clinical Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2077-0383\/15\/3\/948\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T09:07:37Z","timestamp":1769591257000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2077-0383\/15\/3\/948"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,24]]},"references-count":25,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,2]]}},"alternative-id":["jcm15030948"],"URL":"https:\/\/doi.org\/10.3390\/jcm15030948","relation":{},"ISSN":["2077-0383"],"issn-type":[{"value":"2077-0383","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,24]]}}}