{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T01:27:27Z","timestamp":1790386047577,"version":"4.1.0"},"reference-count":72,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T00:00:00Z","timestamp":1772064000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Background\/Objectives: Vitamin B12 deficiency affects up to 40% of certain populations worldwide and has been associated with cardiometabolic risk. However, its relationship with detailed lipoprotein subfractions remains poorly defined. This study aimed to investigate the association between serum vitamin B12 levels and atherogenic lipid profiles using NMR-based lipoprotein subfraction analysis. Methods: In this retrospective cross-sectional study, data from 20,665 apparently healthy adults undergoing routine health screening were analyzed. Participants were categorized into quartiles based on serum vitamin B12 levels (Q1: \u2264328 pg\/mL; Q4: \u2265540 pg\/mL). Lipoprotein subfractions were quantified using Bruker NMR spectroscopy. The Atherogenic Index of Plasma was calculated as log10(triglycerides\/HDL-cholesterol). Statistical analyses included ANCOVA adjusted for age, with corrections for multiple comparisons. Results: Higher serum B12 levels were significantly associated with a more favorable lipid profile. Specifically, AIP values decreased progressively across B12 quartiles (Q1: \u22120.051 \u00b1 0.273; Q4: \u22120.170 \u00b1 0.294; p &lt; 0.001, partial \u03b72 = 0.017). HDL-cholesterol increased (p &lt; 0.001, partial \u03b72 = 0.008), while triglycerides and VLDL-TG subfractions (VLDL1-TG: p &lt; 0.001; VLDL5-TG: p = 0.029) declined with higher B12 levels. Among LDL subfractions, small dense LDL (LDL5-TG) exhibited a consistent inverse association with B12 (p = 0.002, partial \u03b72 = 0.001). These associations were robust across all age strata, with no significant interaction between B12 levels and age. Conclusions: Serum vitamin B12 levels are inversely associated with atherogenic lipid parameters in a large cohort of asymptomatic individuals. Higher B12 status correlates with lower AIP, reduced triglyceride-rich lipoproteins, and diminished small dense LDL particles across all age groups. These findings suggest that B12 status may serve as a potential biomarker in cardiovascular risk assessment and highlight the need for prospective interventional studies.<\/jats:p>","DOI":"10.3390\/jcm15051775","type":"journal-article","created":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T13:58:03Z","timestamp":1772114283000},"page":"1775","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Vitamin B12 Status and Cardiovascular Risk: Novel Insights from NMR-Based Lipoprotein Profiling in 20,665 Adults"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7199-5223","authenticated-orcid":false,"given":"Yunus","family":"Eken","sequence":"first","affiliation":[{"name":"Department of Translational Medicine, Graduate School of Health Sciences, Acibadem Mehmet Ali Aydinlar University, Istanbul 34752, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1922-594X","authenticated-orcid":false,"given":"Nazl\u0131han","family":"Tekin","sequence":"additional","affiliation":[{"name":"Acibadem Labmed Clinical Laboratories, Istanbul 34752, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1296-9711","authenticated-orcid":false,"given":"Furkan","family":"\u015eahin","sequence":"additional","affiliation":[{"name":"Acibadem Labmed Clinical Laboratories, Istanbul 34752, T\u00fcrkiye"},{"name":"Department of Biochemistry and Molecular Biology, Graduate School of Health Sciences, Acibadem Mehmet Ali Aydinlar University, Istanbul 34752, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2599-5871","authenticated-orcid":false,"given":"\u0130rem","family":"Tay","sequence":"additional","affiliation":[{"name":"Acibadem Labmed Clinical Laboratories, Istanbul 34752, T\u00fcrkiye"},{"name":"Department of Biochemistry and Molecular Biology, Graduate School of Health Sciences, Acibadem Mehmet Ali Aydinlar University, Istanbul 34752, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6091-5048","authenticated-orcid":false,"given":"Neslihan","family":"Y\u0131ld\u0131r\u0131m Saral","sequence":"additional","affiliation":[{"name":"Acibadem Labmed Clinical Laboratories, Istanbul 34752, T\u00fcrkiye"},{"name":"Department of Biochemistry and Molecular Biology, Graduate School of Health Sciences, Acibadem Mehmet Ali Aydinlar University, Istanbul 34752, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustafa","family":"Serteser","sequence":"additional","affiliation":[{"name":"Acibadem Labmed Clinical Laboratories, Istanbul 34752, T\u00fcrkiye"},{"name":"Department of Biochemistry and Molecular Biology, Graduate School of Health Sciences, Acibadem Mehmet Ali Aydinlar University, Istanbul 34752, T\u00fcrkiye"},{"name":"Department of Medical Biochemistry, School of Medicine, Acibadem Mehmet Ali Aydinlar University, Istanbul 34752, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8814-7351","authenticated-orcid":false,"given":"Ahmet Tar\u0131k","family":"Baykal","sequence":"additional","affiliation":[{"name":"Acibadem Labmed Clinical Laboratories, Istanbul 34752, T\u00fcrkiye"},{"name":"Department of Biochemistry and Molecular Biology, Graduate School of Health Sciences, Acibadem Mehmet Ali Aydinlar University, Istanbul 34752, T\u00fcrkiye"},{"name":"Department of Medical Biochemistry, School of Medicine, Acibadem Mehmet Ali Aydinlar University, Istanbul 34752, T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Al-Musharaf, S., Aljuraiban, G.S., Danish Hussain, S., Alnaami, A.M., Saravanan, P., and Al-Daghri, N. (2020). Low Serum Vitamin B12 Levels Are Associated with Adverse Lipid Profiles in Apparently Healthy Young Saudi Women. Nutrients, 12.","DOI":"10.3390\/nu12082395"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/s13148-015-0046-8","article-title":"Vitamin B12 insufficiency induces cholesterol biosynthesis by limiting s-adenosylmethionine and modulating the methylation of SREBF1 and LDLR genes","volume":"7","author":"Adaikalakoteswari","year":"2015","journal-title":"Clin. Epigenetics"},{"key":"ref_3","unstructured":"IOM (2025, December 25). Dietary Reference Intakes for Thiamin, Riboflavin, Niacin, Vitamin B6, Folate, Vitamin B12, Pantothenic Acid, Biotin, and Choline, Available online: https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK114310\/."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"17040","DOI":"10.1038\/nrdp.2017.40","article-title":"Vitamin B12 deficiency","volume":"3","author":"Green","year":"2017","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1146\/annurev.biochem.72.121801.161828","article-title":"The many faces of vitamin B12: Catalysis by cobalamin-dependent enzymes","volume":"72","author":"Banerjee","year":"2003","journal-title":"Annu. Rev. Biochem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/0022-510X(84)90147-3","article-title":"The peripheral neuropathy of vitamin B12 deficiency","volume":"66","author":"McCombe","year":"1984","journal-title":"J. Neurol. Sci."},{"key":"ref_7","unstructured":"Rolfes, S.R., Pinna, K., and Whitney, E.N. (2006). Understanding Normal and Clinical Nutrition, Thomson\/Wadsworth."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"5031","DOI":"10.3390\/nu5125031","article-title":"Neuroenhancement with Vitamin B12\u2014Underestimated Neurological Significance","volume":"5","author":"Kisters","year":"2013","journal-title":"Nutrients"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Obeid, R., Andr\u00e8s, E., \u010ce\u0161ka, R., Hooshmand, B., Gu\u00e9ant-Rodriguez, R.-M., Prada, G.I., S\u0142awek, J., Traykov, L., Ta Van, B., and V\u00e1rkonyi, T. (2024). Diagnosis, Treatment and Long-Term Management of Vitamin B12 Deficiency in Adults: A Delphi Expert Consensus. J. Clin. Med., 13.","DOI":"10.20944\/preprints202403.1075.v4"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Ralapanawa, D.M., Jayawickreme, K.P., Ekanayake, E.M., and Jayalath, W.A. (2015). B12 deficiency with neurological manifestations in the absence of anaemia. BMC Res. Notes, 8.","DOI":"10.1186\/s13104-015-1437-9"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"552","DOI":"10.3945\/ajcn.111.015222","article-title":"Monitoring of vitamin B-12 nutritional status in the United States by using plasma methylmalonic acid and serum vitamin B-12","volume":"94","author":"Bailey","year":"2011","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"S238","DOI":"10.1177\/15648265080292S129","article-title":"Conclusions of a WHO Technical Consultation on folate and vitamin B12 deficiencies","volume":"29","year":"2008","journal-title":"Food Nutr. Bull."},{"key":"ref_13","first-page":"1447","article-title":"Algorithm for the early diagnosis of vitamin B12 deficiency in elderly people","volume":"28","author":"Palacios","year":"2013","journal-title":"Nutr. Hosp."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1186\/s12933-014-0129-4","article-title":"Vitamin B12 deficiency is associated with adverse lipid profile in Europeans and Indians with type 2 diabetes","volume":"13","author":"Adaikalakoteswari","year":"2014","journal-title":"Cardiovasc. Diabetol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Knight, B.A., Shields, B.M., Brook, A., Hill, A., Bhat, D.S., Hattersley, A.T., and Yajnik, C.S. (2015). Lower circulating B12 is associated with higher obesity and insulin resistance during pregnancy in a non-diabetic white British population. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0135268"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1042\/bj2320177","article-title":"Regulation of carnitine palmitoyltransferase activity by malonyl-CoA in mitochondria from sheep liver, a tissue with a low capacity for fatty acid synthesis","volume":"232","author":"Brindle","year":"1985","journal-title":"Biochem. J."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1016\/j.febslet.2009.03.038","article-title":"Homocysteine and lipids: S-adenosyl methionine as a key intermediate","volume":"583","author":"Obeid","year":"2009","journal-title":"FEBS Lett."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1007\/s00125-007-0869-8","article-title":"Metabolic programming of offspring by vitamin B12\/folate imbalance during pregnancy","volume":"51","author":"Rosenberg","year":"2008","journal-title":"Diabetologia"},{"key":"ref_19","unstructured":"WHO (2025, December 31). Cardiovascular Diseases (CVDs). Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/cardiovascular-diseases-(cvds)."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Yuan, D., Chu, J., Lin, H., Zhu, G., Qian, J., Yu, Y., Yao, T., Ping, F., Chen, F., and Liu, X. (2022). Mechanism of homocysteine-mediated endothelial injury and its consequences for atherosclerosis. Front. Cardiovasc. Med., 9.","DOI":"10.3389\/fcvm.2022.1109445"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2835","DOI":"10.1001\/jama.1986.03380200073024","article-title":"Incidence of coronary heart disease and lipoprotein cholesterol levels. The Framingham Study","volume":"256","author":"Castelli","year":"1986","journal-title":"JAMA"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Vekic, J., Zeljkovic, A., Cicero, A.F.G., Janez, A., Stoian, A.P., Sonmez, A., and Rizzo, M. (2022). Atherosclerosis Development and Progression: The Role of Atherogenic Small, Dense LDL. Medicina, 58.","DOI":"10.3390\/medicina58020299"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"241","DOI":"10.33594\/000000368","article-title":"Vitamin B12 Induces Hepatic Fatty Infiltration through Altered Fatty Acid Metabolism","volume":"55","author":"Boachie","year":"2021","journal-title":"Cell Physiol. Biochem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3168","DOI":"10.1016\/j.jacc.2018.11.002","article-title":"2018 AHA\/ACC\/AACVPR\/AAPA\/ABC\/ACPM\/ADA\/AGS\/APhA\/ASPC\/NLA\/PCNA Guideline on the Management of Blood Cholesterol: Executive Summary: A Report of the American College of Cardiology\/American Heart Association Task Force on Clinical Practice Guidelines","volume":"73","author":"Grundy","year":"2019","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.nutres.2013.01.002","article-title":"Folic acid and vitamin B12 supplementation lowers plasma homocysteine but has no effect on serum bone turnover markers in elderly women: A randomized, double-blind, placebo-controlled trial","volume":"33","author":"Keser","year":"2013","journal-title":"Nutr. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1079\/PNS2004390","article-title":"B-vitamins, homocysteine metabolism and CVD","volume":"63","author":"Strain","year":"2004","journal-title":"Proc. Nutr. Soc."},{"key":"ref_27","first-page":"1160","article-title":"Vitamin B12 Status and Its Impact on Cardiovascular Risk Factors: Insights from A Hospital Based Cross-Sectional Study","volume":"5","author":"Devi","year":"2023","journal-title":"Int. J. Acad. Med. Pharm."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/1475-2891-14-6","article-title":"Role of homocysteine in the development of cardiovascular disease","volume":"14","author":"Ganguly","year":"2015","journal-title":"Nutr. J."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2457527","DOI":"10.1080\/07853890.2025.2457527","article-title":"Role of hyperhomocysteinemia in atherosclerosis: From bench to bedside","volume":"57","author":"Tian","year":"2025","journal-title":"Ann. Med."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"113640","DOI":"10.1016\/j.biopha.2022.113640","article-title":"Deficiency of B vitamins leads to cholesterol-independent atherogenic transformation of the aorta","volume":"154","author":"Almer","year":"2022","journal-title":"Biomed. Pharmacother."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.jnutbio.2012.01.004","article-title":"Maternal dietary folate and\/or vitamin B12 restrictions alter body composition (adiposity) and lipid metabolism in Wistar rat offspring","volume":"24","author":"Kumar","year":"2013","journal-title":"J. Nutr. Biochem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e11","DOI":"10.1016\/j.amepre.2015.02.009","article-title":"Is vitamin B12 deficiency a risk factor for cardiovascular disease in vegetarians?","volume":"48","author":"Pawlak","year":"2015","journal-title":"Am. J. Prev. Med."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1186\/s12944-025-02779-0","article-title":"Associations of lipoprotein subclasses with all-cause and cardiovascular mortality: Results of two independent cohorts with a 20 year follow-up","volume":"24","author":"Fierfas","year":"2025","journal-title":"Lipids Health Dis."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1093\/eurheartj\/ehz455","article-title":"2019 ESC\/EAS Guidelines for the management of dyslipidaemias: Lipid modification to reduce cardiovascular risk","volume":"41","author":"Mach","year":"2020","journal-title":"Eur. Heart J."},{"key":"ref_35","first-page":"152","article-title":"The new atherogenic plasma index reflects the triglyceride and HDL-cholesterol ratio, the lipoprotein particle size and the cholesterol esterification rate: Changes during lipanor therapy","volume":"46","author":"Frohlich","year":"2000","journal-title":"Vnitr. Lek."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1016\/S0009-9120(01)00263-6","article-title":"The plasma parameter log (TG\/HDL-C) as an atherogenic index: Correlation with lipoprotein particle size and esterification rate in apoB-lipoprotein-depleted plasma (FER(HDL))","volume":"34","author":"Frohlich","year":"2001","journal-title":"Clin. Biochem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1186\/s12933-025-02677-w","article-title":"Association of atherogenic index of plasma trajectory with the incidence of cardiovascular disease over a 12-year follow-up: Findings from the ELSA cohort study","volume":"24","author":"Li","year":"2025","journal-title":"Cardiovasc. Diabetol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1932","DOI":"10.1161\/01.ATV.20.8.1932","article-title":"Race, Visceral Adipose Tissue, Plasma Lipids, and Lipoprotein Lipase Activity in Men and Women","volume":"20","author":"Couillard","year":"2000","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1186\/s12933-022-01522-8","article-title":"Association of the atherogenic index of plasma with cardiovascular risk beyond the traditional risk factors: A nationwide population-based cohort study","volume":"21","author":"Kim","year":"2022","journal-title":"Cardiovasc. Diabetol."},{"key":"ref_40","first-page":"240","article-title":"Atherogenic Index of Plasma (AIP): A marker of cardiovascular disease","volume":"29","author":"Niroumand","year":"2015","journal-title":"Med. J. Islam. Repub. Iran"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/j.jjcc.2012.11.009","article-title":"Vitamin B12 deficiency and hyperhomocysteinemia as correlates of cardiovascular risk factors in Indian subjects with coronary artery disease","volume":"61","author":"Mahalle","year":"2013","journal-title":"J. Cardiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1097\/MOL.0b013e32833b7756","article-title":"Lipoprotein subfractions and cardiovascular disease risk","volume":"21","author":"Krauss","year":"2010","journal-title":"Curr. Opin. Lipidol."},{"key":"ref_43","first-page":"BC16","article-title":"Association of Vitamin B12 with Atherogenic Index of Plasma\u2014A Cross-sectional Study","volume":"15","author":"Bhakta","year":"2021","journal-title":"J. Clin. Diagn. Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1177\/0004563220961753","article-title":"Establishing reference intervals for triglyceride-containing lipoprotein subfraction metabolites measured using nuclear magnetic resonance spectroscopy in a UK population","volume":"58","author":"Joshi","year":"2021","journal-title":"Ann. Clin. Biochem."},{"key":"ref_45","unstructured":"Cohen, J. (1988). Statistical Power Analysis for the Behavioral Sciences, Routledge. [2nd ed.]."},{"key":"ref_46","first-page":"64","article-title":"AIP\u2014Atherogenic index of plasma as a significant predictor of cardiovascular risk: From research to practice","volume":"52","year":"2006","journal-title":"Vnitr. Lek."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"7027624","DOI":"10.1155\/2018\/7027624","article-title":"Understanding the Relationship Between Atherogenic Index of Plasma and Cardiovascular Disease Risk Factors Among Staff of an University in Malaysia","volume":"2018","author":"Bo","year":"2018","journal-title":"J. Nutr. Metab."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1186\/s12933-025-02582-2","article-title":"Atherogenic index of plasma and coronary artery disease: A systematic review and meta-analysis of observational studies","volume":"24","author":"Assempoor","year":"2025","journal-title":"Cardiovasc. Diabetol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1186\/s12944-021-01505-w","article-title":"Visceral adiposity index and atherogenic index of plasma as useful predictors of risk of cardiovascular diseases: Evidence from a cohort study in Iran","volume":"20","author":"Hamzeh","year":"2021","journal-title":"Lipids Health Dis."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1093\/ajcn\/nqy101","article-title":"Are serum concentrations of vitamin B-12 causally related to cardiometabolic risk factors and disease? A Mendelian randomization study","volume":"108","author":"Moen","year":"2018","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"14047","DOI":"10.1038\/s41598-022-18195-8","article-title":"The effects of vitamin B12 supplementation on metabolic profile of patients with non-alcoholic fatty liver disease: A randomized controlled trial","volume":"12","author":"Talari","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.plefa.2015.04.006","article-title":"Vitamin B12 and omega-3 fatty acids together regulate lipid metabolism in Wistar rats","volume":"99","author":"Khaire","year":"2015","journal-title":"Prostaglandins Leukot. Essent. Fat. Acids"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1505","DOI":"10.1161\/CIRCRESAHA.119.312617","article-title":"HDL and Reverse Cholesterol Transport","volume":"124","author":"Ouimet","year":"2019","journal-title":"Circ. Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1007\/978-3-319-70178-3_12","article-title":"Role of Estrogens in the Regulation of Liver Lipid Metabolism","volume":"1043","author":"Palmisano","year":"2017","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1905","DOI":"10.1161\/CIRCULATIONAHA.111.066589","article-title":"Cholesterol efflux and atheroprotection: Advancing the concept of reverse cholesterol transport","volume":"125","author":"Rosenson","year":"2012","journal-title":"Circulation"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1363","DOI":"10.1194\/jlr.R200004-JLR200","article-title":"Metabolic origins and clinical significance of LDL heterogeneity","volume":"43","author":"Berneis","year":"2002","journal-title":"J. Lipid Res."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"e019140","DOI":"10.1161\/JAHA.120.019140","article-title":"Small Dense Low-Density Lipoprotein Cholesterol Is the Most Atherogenic Lipoprotein Parameter in the Prospective Framingham Offspring Study","volume":"10","author":"Ikezaki","year":"2021","journal-title":"J. Am. Heart Assoc."},{"key":"ref_58","first-page":"6","article-title":"Small LDL subfractions are associated with coronary atherosclerosis despite no differences in conventional lipids","volume":"55","author":"Klevjer","year":"2022","journal-title":"Physiol. Genom."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"2122","DOI":"10.1016\/j.jacc.2020.02.059","article-title":"Triglyceride-Rich Lipoprotein Cholesterol, Small Dense LDL Cholesterol, and Incident Cardiovascular Disease","volume":"75","author":"Duran","year":"2020","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_60","first-page":"264","article-title":"Evaluation of lipid parameters in patients receiving vitamin B12 therapy","volume":"20","author":"Sezgin","year":"2019","journal-title":"\u0130stanb. Med. J."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1093\/ajcn\/71.2.514","article-title":"Plasma vitamin B-12 concentrations relate to intake source in the Framingham Offspring study","volume":"71","author":"Tucker","year":"2000","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1007\/s11883-000-0021-4","article-title":"Triglyceride, small, dense low-density lipoprotein, and the atherogenic lipoprotein phenotype","volume":"2","author":"Austin","year":"2000","journal-title":"Curr. Atheroscler. Rep."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1194\/jlr.R027011","article-title":"New molecular insights into CETP structure and function: A review","volume":"53","author":"Charles","year":"2012","journal-title":"J. Lipid Res."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"693S","DOI":"10.3945\/ajcn.2008.26947A","article-title":"How common is vitamin B-12 deficiency?","volume":"89","author":"Allen","year":"2009","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1161\/ATVBAHA.107.159228","article-title":"Abdominal Obesity and the Metabolic Syndrome: Contribution to Global Cardiometabolic Risk","volume":"28","author":"Lemieux","year":"2008","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"1800","DOI":"10.1161\/01.ATV.17.9.1800","article-title":"Importance of Estrogen Receptors in Hepatic LDL Receptor Regulation","volume":"17","author":"Parini","year":"1997","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.1056\/NEJM199110243251702","article-title":"Effects of Postmenopausal Estrogen Replacement on the Concentrations and Metabolism of Plasma Lipoproteins","volume":"325","author":"Schiff","year":"1991","journal-title":"N. Engl. J. Med."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1126","DOI":"10.1007\/s001250051503","article-title":"Contribution of visceral obesity to the deterioration of the metabolic risk profile in men with impaired glucose tolerance","volume":"43","author":"Pascot","year":"2000","journal-title":"Diabetologia"},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Varlamov, O., Bethea, C.L., and Roberts, C.T. (2015). Sex-Specific Differences in Lipid and Glucose Metabolism. Front. Endocrinol., 5.","DOI":"10.3389\/fendo.2014.00241"},{"key":"ref_70","first-page":"e596","article-title":"2019 ACC\/AHA Guideline on the Primary Prevention of Cardiovascular Disease: A Report of the American College of Cardiology\/American Heart Association Task Force on Clinical Practice Guidelines","volume":"140","author":"Arnett","year":"2019","journal-title":"Circulation"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1097\/MCA.0000000000001375","article-title":"Management of dyslipidemia in coronary artery disease: The present and the future","volume":"35","author":"Dimitriadis","year":"2024","journal-title":"Coron. Artery Dis."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1325","DOI":"10.1001\/jama.2015.2274","article-title":"Efficacy of folic acid therapy in primary prevention of stroke among adults with hypertension in China: The CSPPT randomized clinical trial","volume":"313","author":"Huo","year":"2015","journal-title":"JAMA"}],"container-title":["Journal of Clinical Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2077-0383\/15\/5\/1775\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T14:21:50Z","timestamp":1772115710000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2077-0383\/15\/5\/1775"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,26]]},"references-count":72,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2026,3]]}},"alternative-id":["jcm15051775"],"URL":"https:\/\/doi.org\/10.3390\/jcm15051775","relation":{},"ISSN":["2077-0383"],"issn-type":[{"value":"2077-0383","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,26]]}}}