{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T17:15:26Z","timestamp":1778778926491,"version":"3.51.4"},"reference-count":47,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T00:00:00Z","timestamp":1778716800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Background: Vitamin K (VK) has emerging roles beyond coagulation, including potential involvement in glucose metabolism. While large cohorts report inverse associations between VK status and incident type 2 diabetes, less is known about its relationship with insulin resistance markers in general populations. Methods: We conducted a cross-sectional analysis of 23,247 adults from the National Health and Nutrition Examination Survey (NHANES) 2001\u20132018. Dietary VK intake was assessed using a single 24 h recall and modeled as energy-adjusted nutrient density (\u00b5g\/1000 kcal). Fasting insulin, homeostasis model assessment of insulin resistance (HOMA-IR), fasting glucose, and HbA1c were analyzed using survey-weighted linear regression; models were adjusted for demographic, socioeconomic, lifestyle, and adiposity-related factors. Effect modification by baseline metabolic status was evaluated, and sensitivity analyses assessed robustness. Results: Higher energy-adjusted VK intake was modestly associated with lower fasting insulin and HOMA-IR, but not with fasting glucose or HbA1c in primary linear models. Each SD increase in VK intake was associated with 1.4% lower fasting insulin (95% CI: \u22122.4% to \u22120.4%) and 1.3% lower HOMA-IR (95% CI: \u22122.3% to \u22120.3%) in fully adjusted models. Associations were attenuated following adjustment for adiposity. Effect modification by baseline metabolic status was observed, with stronger associations among normoglycemic individuals. Findings were consistent across sensitivity analyses, although effect sizes were small. Conclusions: In this nationally representative sample of U.S. adults, higher VK intake was modestly associated with lower insulin resistance markers. Prospective studies incorporating objective VK biomarkers and dynamic measures of insulin sensitivity are needed to clarify these associations.<\/jats:p>","DOI":"10.3390\/jcm15103763","type":"journal-article","created":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T16:27:56Z","timestamp":1778776076000},"page":"3763","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dietary Vitamin K Intake and Insulin Resistance Markers in U.S. Adults: NHANES 2001\u20132018"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8279-0373","authenticated-orcid":false,"given":"Wojciech","family":"Matuszewski","sequence":"first","affiliation":[{"name":"Clinic of Endocrinology and Metabolic Diseases, School of Medicine, Collegium Medicum, University of Warmia and Mazury in Olsztyn, 10-957 Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-4793-8711","authenticated-orcid":false,"given":"Miko\u0142aj","family":"Madeksza","sequence":"additional","affiliation":[{"name":"Clinic of Endocrinology and Metabolic Diseases, School of Medicine, Collegium Medicum, University of Warmia and Mazury in Olsztyn, 10-957 Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Micha\u0142","family":"Szklarz","sequence":"additional","affiliation":[{"name":"Clinic of Endocrinology and Metabolic Diseases, School of Medicine, Collegium Medicum, University of Warmia and Mazury in Olsztyn, 10-957 Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulina","family":"W\u0142odarczyk","sequence":"additional","affiliation":[{"name":"Clinic of Endocrinology and Metabolic Diseases, School of Medicine, Collegium Medicum, University of Warmia and Mazury in Olsztyn, 10-957 Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrycja","family":"Wa\u015bniewska","sequence":"additional","affiliation":[{"name":"Clinic of Endocrinology and Metabolic Diseases, School of Medicine, Collegium Medicum, University of Warmia and Mazury in Olsztyn, 10-957 Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8665-6606","authenticated-orcid":false,"given":"Judyta","family":"Juranek","sequence":"additional","affiliation":[{"name":"Department of Human Physiology and Pathophysiology, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Ho, H.-J., Komai, M., and Shirakawa, H. (2020). Beneficial Effects of Vitamin K Status on Glycemic Regulation and Diabetes Mellitus: A Mini-Review. Nutrients, 12.","DOI":"10.3390\/nu12082485"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1002\/biof.1569","article-title":"A Systematic Review on the Mechanisms of Vitamin K Effects on the Complications of Diabetes and Pre-diabetes","volume":"46","author":"Karamzad","year":"2020","journal-title":"BioFactors"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/s12986-023-00779-4","article-title":"Revisiting the Interconnection between Lipids and Vitamin K Metabolism: Insights from Recent Research and Potential Therapeutic Implications: A Review","volume":"21","author":"Tan","year":"2024","journal-title":"Nutr. Metab."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Matuszewski, W., Madeksza, M., Szklarz, M., Rutkiewicz, A., Rutkowska, J., and Harazny, J.M. (2026). Vitamin K as an Endocrine Modulator: Mechanistic Links to Glucose Metabolism and Beyond. Nutrients, 18.","DOI":"10.20944\/preprints202603.1015.v1"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Ka\u017amierczak-Bara\u0144ska, J., and Karwowski, B.T. (2022). Vitamin K Contribution to DNA Damage\u2014Advantage or Disadvantage? A Human Health Response. Nutrients, 14.","DOI":"10.3390\/nu14204219"},{"key":"ref_6","first-page":"5246","article-title":"Menadione Induces G2\/M Arrest in Gastric Cancer Cells by down-Regulation of CDC25C and Proteasome Mediated Degradation of CDK1 and Cyclin B1","volume":"8","author":"Lee","year":"2016","journal-title":"Am. J. Transl. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1080\/07315724.2009.10718099","article-title":"Dietary Phylloquinone Intakes and Metabolic Syndrome in US Young Adults","volume":"28","author":"Pan","year":"2009","journal-title":"J. Am. Coll. Nutr."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"889","DOI":"10.3945\/ajcn.2009.27930","article-title":"Vitamin K, an Example of Triage Theory: Is Micronutrient Inadequacy Linked to Diseases of Aging?","volume":"90","author":"McCann","year":"2009","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"5329","DOI":"10.3402\/fnr.v56i0.5329","article-title":"Vitamin K: The Effect on Health beyond Coagulation\u2014An Overview","volume":"56","author":"Vermeer","year":"2012","journal-title":"Food Nutr. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"220","DOI":"10.2337\/db18-0543","article-title":"Circulating Phylloquinone Concentrations and Risk of Type 2 Diabetes: A Mendelian Randomization Study","volume":"68","author":"Zwakenberg","year":"2019","journal-title":"Diabetes"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1699","DOI":"10.2337\/dc09-2302","article-title":"Dietary Phylloquinone and Menaquinones Intakes and Risk of Type 2 Diabetes","volume":"33","author":"Beulens","year":"2010","journal-title":"Diabetes Care"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1113","DOI":"10.3945\/ajcn.111.033498","article-title":"Dietary Phylloquinone Intake and Risk of Type 2 Diabetes in Elderly Subjects at High Risk of Cardiovascular Disease","volume":"96","year":"2012","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2472","DOI":"10.1210\/jc.2014-4449","article-title":"Association Between Vitamin K and the Metabolic Syndrome: A 10-Year Follow-Up Study in Adults","volume":"100","author":"Dam","year":"2015","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.3945\/jn.109.118380","article-title":"Adulthood Obesity Is Positively Associated with Adipose Tissue Concentrations of Vitamin K and Inversely Associated with Circulating Indicators of Vitamin K Status in Men and Women","volume":"140","author":"Shea","year":"2010","journal-title":"J. Nutr."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1017\/S000711451100626X","article-title":"Association of Vitamin K Status with Adiponectin and Body Composition in Healthy Subjects: Uncarboxylated Osteocalcin Is Not Associated with Fat Mass and Body Weight","volume":"108","author":"Knapen","year":"2012","journal-title":"Br. J. Nutr."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.nutres.2025.12.001","article-title":"Dietary Vitamins A and K Are Inversely Associated with Visceral Adiposity in US Adults: NHANES 2011\u20132018","volume":"146","author":"Villa","year":"2026","journal-title":"Nutr. Res."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1515\/cclm-2020-0194","article-title":"Overweight-Obesity Is Associated with Decreased Vitamin K2 Levels in Hemodialysis Patients","volume":"59","author":"Ravera","year":"2021","journal-title":"Clin. Chem. Lab. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1093\/aje\/kwm306","article-title":"Vitamin K and Vitamin D Status: Associations with Inflammatory Markers in the Framingham Offspring Study","volume":"167","author":"Shea","year":"2008","journal-title":"Am. J. Epidemiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.3945\/jn.111.139634","article-title":"Circulating Uncarboxylated Matrix Gla Protein Is Associated with Vitamin K Nutritional Status, but Not Coronary Artery Calcium, in Older Adults","volume":"141","author":"Shea","year":"2011","journal-title":"J. Nutr."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"4866","DOI":"10.3748\/wjg.v26.i32.4866","article-title":"Inactive Matrix Gla Protein Is Elevated in Patients with Inflammatory Bowel Disease","volume":"26","author":"Brnic","year":"2020","journal-title":"World J. Gastroenterol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Roumeliotis, S., Roumeliotis, A., Stamou, A., Leivaditis, K., Kantartzi, K., Panagoutsos, S., and Liakopoulos, V. (2020). The Association of Dp-ucMGP with Cardiovascular Morbidity and Decreased Renal Function in Diabetic Chronic Kidney Disease. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21176035"},{"key":"ref_22","first-page":"EP368","article-title":"Is MGP an Inflammatory Marker?","volume":"81","author":"Schweighofer","year":"2022","journal-title":"Endocr. Abstr."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Luo, W., Ye, D., Zhao, K., Zhou, L., Wu, Y., and Ge, Q. (2025). Associations between Vitamin K and Systemic Immune and Inflammation Biomarkers: A Population-Based Study from the NHANES (2007\u20132020). Front. Nutr., 12.","DOI":"10.3389\/fnut.2025.1625209"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2974","DOI":"10.1039\/D3FO04614A","article-title":"The Association between Vitamin K Intake and Dyslipidemia in US Adults: The Mediating Effect of Insulin Resistance","volume":"15","author":"Wang","year":"2024","journal-title":"Food Funct."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Ho, H.-J., Shirakawa, H., Hirahara, K., Sone, H., Kamiyama, S., and Komai, M. (2019). Menaquinone-4 Amplified Glucose-Stimulated Insulin Secretion in Isolated Mouse Pancreatic Islets and INS-1 Rat Insulinoma Cells. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20081995"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"112500","DOI":"10.1016\/j.celrep.2023.112500","article-title":"Vitamin K-Dependent Carboxylation Regulates Ca2+ Flux and Adaptation to Metabolic Stress in \u03b2 Cells","volume":"42","author":"Lacombe","year":"2023","journal-title":"Cell Rep."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Staudinger, J.L., Mahroke, A., Patel, G., Dattel, C., and Reddy, S. (2024). Pregnane X Receptor Signaling Pathway and Vitamin K: Molecular Mechanisms and Clinical Relevance in Human Health. Cells, 13.","DOI":"10.3390\/cells13080681"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Kieronska-Rudek, A., Kij, A., Kaczara, P., Tworzydlo, A., Napiorkowski, M., Sidoryk, K., and Chlopicki, S. (2021). Exogenous Vitamins K Exert Anti-Inflammatory Effects Dissociated from Their Role as Substrates for Synthesis of Endogenous MK-4 in Murine Macrophages Cell Line. Cells, 10.","DOI":"10.3390\/cells10071571"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"450","DOI":"10.1039\/C7FO01491K","article-title":"Prophylactic Role of Vitamin K Supplementation on Vascular Inflammation in Type 2 Diabetes by Regulating the NF-\u03baB\/Nrf2 Pathway via Activating Gla Proteins","volume":"9","author":"Dihingia","year":"2018","journal-title":"Food Funct."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2460","DOI":"10.1182\/blood-2013-09-528752","article-title":"TAM Receptors, Gas6, and Protein S: Roles in Inflammation and Hemostasis","volume":"123","year":"2014","journal-title":"Blood"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1997","DOI":"10.1002\/jnr.22029","article-title":"Vitamin K Prevents Oxidative Cell Death by Inhibiting Activation of 12-lipoxygenase in Developing Oligodendrocytes","volume":"87","author":"Li","year":"2009","journal-title":"J. Neurosci. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.jnutbio.2017.09.022","article-title":"Vitamin K1 Inversely Correlates with Glycemia and Insulin Resistance in Patients with Type 2 Diabetes (T2D) and Positively Regulates SIRT1\/AMPK Pathway of Glucose Metabolism in Liver of T2D Mice and Hepatocytes Cultured in High Glucose","volume":"52","author":"Dihingia","year":"2018","journal-title":"J. Nutr. Biochem."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1089\/ars.2019.7908","article-title":"Vitamin K2 Alleviates Insulin Resistance in Skeletal Muscle by Improving Mitochondrial Function via SIRT1 Signaling","volume":"34","author":"Su","year":"2021","journal-title":"Antioxid. Redox Signal."},{"key":"ref_34","unstructured":"Centi, A.J. (2015). Association of Vitamin K with Insulin Resistance and Body Composition. [Ph.D. Dissertation, Tufts University]."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1528","DOI":"10.3945\/ajcn.2010.30108","article-title":"Effect of Phylloquinone Supplementation on Glucose Homeostasis in Humans","volume":"92","author":"Kumar","year":"2010","journal-title":"Am. J. Clin. Nutr."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1160\/TH14-08-0675","article-title":"Menaquinone-7 Supplementation Improves Arterial Stiffness in Healthy Postmenopausal Women: A Double-Blind Randomised Clinical Trial","volume":"113","author":"Knapen","year":"2015","journal-title":"Thromb. Haemost."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1055\/s-0044-100616","article-title":"Effect of Vitamin K Supplementation on Glycemic Control: A Systematic Review and Meta-Analysis of Clinical Trials","volume":"50","author":"Shahdadian","year":"2018","journal-title":"Horm. Metab. Res."},{"key":"ref_38","first-page":"186","article-title":"The Effect of Vitamin K Supplementation on Glycemic Indices in Adults: A Systematic Review and Meta- Analysis of Clinical Trials","volume":"10","author":"Khatapoush","year":"2025","journal-title":"Int. J. Nutr. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"106970","DOI":"10.1016\/j.prostaglandins.2025.106970","article-title":"Effect of Menaquinone-7 (MK-7) Supplementation on Anthropometric Measurements, Glycemic Indices, and Lipid Profiles: A Systematic Review and Meta-Analysis of Randomized Controlled Trials","volume":"177","author":"Nikpayam","year":"2025","journal-title":"Prostaglandins Other Lipid Mediat."},{"key":"ref_40","first-page":"316","article-title":"The Effects of Calcium, Vitamins D and K Co-Supplementation on Markers of Insulin Metabolism and Lipid Profiles in Vitamin D-Deficient Women with Polycystic Ovary Syndrome","volume":"125","author":"Karamali","year":"2017","journal-title":"Exp. Clin. Endocrinol. Diabetes Off. J. Ger. Soc. Endocrinol. Ger. Diabetes Assoc."},{"key":"ref_41","unstructured":"Centers for Disease Control and Prevention (2026, January 30). NHANES NCHS Ethics Review Board Approval 1999\u20132022, Available online: https:\/\/www.cdc.gov\/nchs\/nhanes\/about\/erb.html."},{"key":"ref_42","unstructured":"Centers for Disease Control and Prevention (2026, January 31). NHANES Survey Methods and Analytic Guidelines, Available online: https:\/\/wwwn.cdc.gov\/nchs\/nhanes\/analyticguidelines.aspx."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Wu, J., Shi, Y., Zhou, M., Chen, M., Ji, S., Liu, X., Zhou, M., Xia, R., Zheng, X., and Wang, W. (2024). Nutrient Vitamins Enabled Metabolic Regulation of Ferroptosis via Reactive Oxygen Species Biology. Front. Pharmacol., 15.","DOI":"10.3389\/fphar.2024.1434088"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1038\/s41586-022-05022-3","article-title":"A Non-Canonical Vitamin K Cycle Is a Potent Ferroptosis Suppressor","volume":"608","author":"Mishima","year":"2022","journal-title":"Nature"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1007\/s00018-022-04416-w","article-title":"Vitamin K1 Inhibits Ferroptosis and Counteracts a Detrimental Effect of Phenprocoumon in Experimental Acute Kidney Injury","volume":"79","author":"Kolbrink","year":"2022","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1079\/BJN19900150","article-title":"Dietary Modification of Potential Vitamin K Supply from Enteric Bacterial Menaquinones in Rats","volume":"63","author":"Mathers","year":"1990","journal-title":"Br. J. Nutr."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Shea, M., and Booth, S. (2016). Concepts and Controversies in Evaluating Vitamin K Status in Population-Based Studies. Nutrients, 8.","DOI":"10.3390\/nu8010008"}],"container-title":["Journal of Clinical Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2077-0383\/15\/10\/3763\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T16:47:41Z","timestamp":1778777261000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2077-0383\/15\/10\/3763"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,14]]},"references-count":47,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2026,5]]}},"alternative-id":["jcm15103763"],"URL":"https:\/\/doi.org\/10.3390\/jcm15103763","relation":{},"ISSN":["2077-0383"],"issn-type":[{"value":"2077-0383","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,14]]}}}