{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:51:07Z","timestamp":1787028667947,"version":"build-2736575974"},"reference-count":34,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T00:00:00Z","timestamp":1779408000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Austrian MS Research Society"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Objective: We aimed to assess whether modified OCT acquisition parameters improve GCIPL measurement reliability. Methods: Participants with multiple sclerosis (PwMS) and age- and sex-matched healthy controls (HC) underwent OCT (Spectralis OCT, Heidelberg Engineering, Heidelberg, Germany) at baseline and after two and four weeks. At each visit, five macular scan protocols were acquired: standard (49 lines, high-speed [HS], automated real-time tracking [ART]: 9), high-ART (49 lines, HS, ART: 50), high-lines (97 lines, HS, ART: 9), high-resolution (49 lines, high-resolution [HR], ART: 9), and maximum (97 lines, HR, ART: 50). Reliability was assessed using intraclass correlation coefficients (ICC). Absolute test\u2013retest reproducibility was quantified using the mean absolute difference (MAD). Results: Thirty-eight eyes from nine PwMS (mean age 34.1 \u00b1 8.0 years, 44.4% female) and ten HC (31.7 \u00b1 11.1 years, 50.0% female) were included. At baseline, mean GCIPL thickness ranged from 70.8 \u00b5m to 71.5 \u00b5m across protocols, demonstrating excellent inter-protocol agreement (ICC 0.99; 95% confidence interval [CI]: 0.98\u20130.99; p &lt; 0.001) and only marginally higher values with increased ART. Test\u2013retest reliability was excellent for all protocols, demonstrating marginally lower absolute measurement variability of high-ART and high-lines protocols (MAD 0.26\u20130.27; 95% CI: 0.21\u20130.32), while temporal agreement remained excellent and comparable across acquisition settings. Mean acquisition time ranged from 10.6 \u00b1 1.6 s for the standard protocol to 231.9 \u00b1 36.4 s for the maximum protocol. Conclusions: All OCT acquisition protocols demonstrated excellent inter-protocol and test\u2013retest reliability for GCIPL measurements. The high-lines protocol provides the most favourable balance between measurement reliability and acquisition time, supporting its potential utility for longitudinal GCIPL monitoring.<\/jats:p>","DOI":"10.3390\/jcm15113999","type":"journal-article","created":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T09:17:09Z","timestamp":1779441429000},"page":"3999","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Reliability of Macular Ganglion Cell-Inner Plexiform Layer Thickness Measurements Across Scan Protocols in Spectral-Domain Optical Coherence Tomography"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4146-5870","authenticated-orcid":false,"given":"Nik","family":"Krajnc","sequence":"first","affiliation":[{"name":"Department of Neurology, Medical University of Vienna, Waehringer Guertel 18\u201320, 1090 Vienna, Austria"},{"name":"Comprehensive Center for Clinical Neurosciences & Mental Health, Medical University of Vienna, Waehringer Guertel 18\u201320, 1090 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-2725-6710","authenticated-orcid":false,"given":"Martin","family":"Bertich","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Optometry, Medical University of Vienna, Waehringer Guertel 18\u201320, 1090 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabian","family":"F\u00f6ttinger","sequence":"additional","affiliation":[{"name":"Department of Neurology, Medical University of Vienna, Waehringer Guertel 18\u201320, 1090 Vienna, Austria"},{"name":"Comprehensive Center for Clinical Neurosciences & Mental Health, Medical University of Vienna, Waehringer Guertel 18\u201320, 1090 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Macher","sequence":"additional","affiliation":[{"name":"Department of Neurology, Medical University of Vienna, Waehringer Guertel 18\u201320, 1090 Vienna, Austria"},{"name":"Comprehensive Center for Clinical Neurosciences & Mental Health, Medical University of Vienna, Waehringer Guertel 18\u201320, 1090 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felix Konstantin","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Department of Neurology, Medical University of Vienna, Waehringer Guertel 18\u201320, 1090 Vienna, Austria"},{"name":"Comprehensive Center for Clinical Neurosciences & Mental Health, Medical University of Vienna, Waehringer Guertel 18\u201320, 1090 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Stapf","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Optometry, Medical University of Vienna, Waehringer Guertel 18\u201320, 1090 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0569-0229","authenticated-orcid":false,"given":"Berthold","family":"Pemp","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Optometry, Medical University of Vienna, Waehringer Guertel 18\u201320, 1090 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0825-0851","authenticated-orcid":false,"given":"Gabriel","family":"Bsteh","sequence":"additional","affiliation":[{"name":"Department of Neurology, Medical University of Vienna, Waehringer Guertel 18\u201320, 1090 Vienna, Austria"},{"name":"Comprehensive Center for Clinical Neurosciences & Mental Health, Medical University of Vienna, Waehringer Guertel 18\u201320, 1090 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,5,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1001\/jamaophthalmol.2023.0005","article-title":"Association of Rates of Ganglion Cell and Inner Plexiform Thinning With Development of Glaucoma in Eyes With Suspected Glaucoma","volume":"141","author":"Mohammadzadeh","year":"2023","journal-title":"JAMA Ophthalmol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1748","DOI":"10.1038\/s41433-020-01152-y","article-title":"Changes in the structure of retinal layers over time in non-arteritic anterior ischaemic optic neuropathy","volume":"35","author":"Lee","year":"2021","journal-title":"Eye"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e214069","DOI":"10.1212\/WNL.0000000000214069","article-title":"Association Between Retinal Layer Atrophy With Clinical and Radiologic Progression in People With 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