{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T11:01:40Z","timestamp":1781002900486,"version":"3.54.1"},"reference-count":41,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T00:00:00Z","timestamp":1780704000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Background: Full-mouth disinfection (FMD) has been proposed as an effective non-surgical approach for the management of periodontitis; however, its clinical and microbiological outcomes in patients with depressive comorbidity remain insufficiently explored. Methods: This prospective study included 80 patients diagnosed with stage II periodontitis, allocated into two groups based on the presence or absence of depressive disorder. All participants underwent standardized FMD. Clinical parameters, including bleeding on probing and plaque index, together with subgingival bacterial load (total bacterial load, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia), were assessed at baseline and 12 weeks post-treatment using quantitative polymerase chain reaction. Microbiological data were log10-transformed prior to statistical analysis. Results: Significant reductions in both clinical and microbiological parameters were observed following treatment in both groups. Improvements in bleeding on probing and plaque index were accompanied by a marked decrease in total bacterial load and in the targeted periodontal pathogens. Patients with depressive disorder exhibited a higher baseline microbial burden; however, post-treatment reductions were comparable between groups. Moderate positive correlations were identified between total bacterial load and clinical parameters. Conclusions: FMD was associated with substantial short-term improvements in both clinical and microbiological outcomes in patients with stage II periodontitis. Depressive comorbidity did not appear to adversely influence treatment response. These findings support the role of biofilm control as a central component of periodontal therapy across different patient profiles.<\/jats:p>","DOI":"10.3390\/jcm15124402","type":"journal-article","created":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T05:43:46Z","timestamp":1780897426000},"page":"4402","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative Analysis of Subgingival Microbial Profiles in Patients with Periodontal Disease with and Without Depressive Comorbidity"],"prefix":"10.3390","volume":"15","author":[{"given":"Bogdan-Constantin","family":"Vasiliu","sequence":"first","affiliation":[{"name":"Grigore T. Popa University of Medicine and Pharmacy, 16 Universitatii Str., 700115 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Tatarciuc","sequence":"additional","affiliation":[{"name":"Grigore T. Popa University of Medicine and Pharmacy, 16 Universitatii Str., 700115 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5092-5234","authenticated-orcid":false,"given":"Maria Alexandra","family":"Martu","sequence":"additional","affiliation":[{"name":"Grigore T. Popa University of Medicine and Pharmacy, 16 Universitatii Str., 700115 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9167-6302","authenticated-orcid":false,"given":"Mihaela Monica","family":"Scutariu","sequence":"additional","affiliation":[{"name":"Grigore T. Popa University of Medicine and Pharmacy, 16 Universitatii Str., 700115 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doriana","family":"Agop Forna","sequence":"additional","affiliation":[{"name":"Grigore T. Popa University of Medicine and Pharmacy, 16 Universitatii Str., 700115 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0017-9672","authenticated-orcid":false,"given":"Ionu\u021b","family":"Luchian","sequence":"additional","affiliation":[{"name":"Grigore T. Popa University of Medicine and Pharmacy, 16 Universitatii Str., 700115 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9588-6910","authenticated-orcid":false,"given":"Sorina Mihaela","family":"Solomon","sequence":"additional","affiliation":[{"name":"Grigore T. Popa University of Medicine and Pharmacy, 16 Universitatii Str., 700115 Iasi, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"435","DOI":"10.2174\/1874210601812010435","article-title":"Twenty years of full-mouth disinfection: The past, the present and the future","volume":"12","author":"Pockpa","year":"2018","journal-title":"Open Dent. J."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1111\/j.1600-051X.2008.01356.x","article-title":"Relation of body mass index, periodontitis and Tannerella forsythia","volume":"36","author":"Haffajee","year":"2009","journal-title":"J. Clin. Periodontol."},{"key":"ref_3","first-page":"S173","article-title":"Periodontitis: Consensus report of workgroup 2 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions","volume":"89","author":"Papapanou","year":"2018","journal-title":"J. Periodontol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1038\/nri3785","article-title":"Periodontitis: From microbial immune subversion to systemic inflammation","volume":"15","author":"Hajishengallis","year":"2015","journal-title":"Nat. Rev. Immunol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Fine, D.H., Patil, A.G., and Velusamy, S.K. (2019). Aggregatibacter actinomycetemcomitans (Aa) under the radar: Myths and misunderstandings of Aa and its role in aggressive periodontitis. Front. Immunol., 10.","DOI":"10.3389\/fimmu.2019.00728"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"How, K.Y., Song, K.P., and Chan, K.G. (2016). Porphyromonas gingivalis: An overview of periodontopathic pathogen below the gum line. Front. Microbiol., 7.","DOI":"10.3389\/fmicb.2016.00053"},{"key":"ref_7","first-page":"95","article-title":"Virulence factors of the oral spirochete Treponema denticola","volume":"96","author":"Dashper","year":"2017","journal-title":"J. Dent. Res."},{"key":"ref_8","first-page":"1","article-title":"Infectious risks for oral implants: A review of the literature","volume":"3","author":"Quirynen","year":"2015","journal-title":"Clin. Oral Implant. Res."},{"key":"ref_9","first-page":"7","article-title":"Periodontal risk assessment (PRA) for patients in supportive periodontal therapy (SPT)","volume":"1","author":"Lang","year":"2015","journal-title":"Oral Health Prev. Dent."},{"key":"ref_10","first-page":"123","article-title":"Effectiveness of periodontal laser therapy in the treatment of chronic periodontitis: A systematic review and meta-analysis","volume":"33","author":"Sgolastra","year":"2018","journal-title":"Lasers Med. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Van der Weijden, G.A., and van Loveren, C. (2023). Mechanical plaque removal in step-1 of care. Periodontol. 2000, Online ahead of print.","DOI":"10.1111\/prd.12541"},{"key":"ref_12","first-page":"910","article-title":"The prevalence and severity of periodontal disease in Mainland China: Data from the Fourth National Oral Health Survey (2015\u20132016)","volume":"44","author":"Jiao","year":"2017","journal-title":"J. Clin. Periodontol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"732","DOI":"10.2174\/1570159X14666151208113700","article-title":"Inflammation in depression and the potential for anti-inflammatory treatment","volume":"14","author":"Kohler","year":"2016","journal-title":"Curr. Neuropharmacol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1111\/prd.12036","article-title":"Role of chronic stress and depression in periodontal diseases","volume":"64","author":"Warren","year":"2014","journal-title":"Periodontol. 2000"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.biopsych.2011.09.023","article-title":"Cumulative depression episodes predict later C-reactive protein levels: A prospective analysis","volume":"71","author":"Copeland","year":"2012","journal-title":"Biol. Psychiatry"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"175","DOI":"10.18662\/brain\/13.1Sup1\/311","article-title":"Evaluation of the salivary flow in patients with schizophrenia: A literature review","volume":"13","author":"Burlea","year":"2022","journal-title":"Brain Broad Res. Artif. Intell. Neurosci."},{"key":"ref_17","first-page":"1723","article-title":"Systemic and local effects of antidepressants on oral health","volume":"25","author":"Hagen","year":"2019","journal-title":"Oral Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1177\/1753425908101920","article-title":"Detection and quantification of five major periodontal pathogens by single copy gene-based real-time PCR","volume":"15","author":"Laitinen","year":"2009","journal-title":"Innate Immun."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1902\/jop.2015.150105","article-title":"Scaling and root planing per quadrant versus one-stage full-mouth disinfection: Assessment of the impact of chronic periodontitis treatment on quality of life\u2014A clinical randomized, controlled trial","volume":"87","author":"Santuchi","year":"2016","journal-title":"J. Periodontol."},{"key":"ref_20","first-page":"S106","article-title":"Diabetes and periodontal diseases: Consensus report of the Joint EFP\/AAP Workshop on Periodontitis and Systemic Diseases","volume":"40","author":"Chapple","year":"2015","journal-title":"J. Clin. Periodontol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1111\/jre.12605","article-title":"Periodontal condition and levels of bacteria associated with periodontitis in individuals with bipolar affective disorders: A case-control study","volume":"54","author":"Cunha","year":"2019","journal-title":"J. Periodontal Res."},{"key":"ref_22","first-page":"83","article-title":"Infection or inflammation: The link between periodontal and cardiovascular diseases","volume":"10","author":"Seymour","year":"2014","journal-title":"Future Cardiol."},{"key":"ref_23","first-page":"249","article-title":"Oral biofilms, oral and periodontal infections, and systemic disease","volume":"26","author":"Maddi","year":"2013","journal-title":"Am. J. Dent."},{"key":"ref_24","first-page":"119","article-title":"The oral health of people with anxiety and depressive disorders\u2014A systematic review and meta-analysis","volume":"188","author":"Kisely","year":"2015","journal-title":"J. Affect. Disord."},{"key":"ref_25","first-page":"CD004714","article-title":"Treatment of periodontal disease for glycaemic control in people with diabetes mellitus","volume":"11","author":"Simpson","year":"2016","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_26","first-page":"232","article-title":"Prevalence of anaerobic bacteria (P. gingivalis) as major microbial agent in the incidence of periodontal diseases by meta-analysis","volume":"19","author":"Rafiei","year":"2018","journal-title":"J. Dent."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1202","DOI":"10.5005\/jp-journals-10024-1477","article-title":"Microbial flora in oral diseases","volume":"14","author":"Patil","year":"2013","journal-title":"J. Contemp. Dent. Pract."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Dubar, M., Clerc-Urm\u00e8s, I., Baumann, C., Cl\u00e9ment, C., Alauzet, C., and Bisson, C. (2020). Relations of psychosocial factors and cortisol with periodontal and bacterial parameters: A prospective clinical study in 30 patients with periodontitis before and after non-surgical treatment. Int. J. Environ. Res. Public Health, 17.","DOI":"10.3390\/ijerph17207651"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Teodorescu, H.N., Andrian, S., Ghiorghe, C.A., Ghel\u021bu, S., and T\u0103r\u0103boan\u021b\u0103, I. (2021, January 13). Speech under combined schizophrenia and salivary flow alterations\u2013Preliminary data and results. Proceedings of the 2021 International Conference on Speech Technology and Human-Computer Dialogue (SpeD), Virtual.","DOI":"10.1109\/SpeD53181.2021.9587347"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Budala, D.G., Luchian, I., Virvescu, D.I., Tudorici, T., Constantin, V., Surlari, Z., Butnaru, O., Bosinceanu, D.N., Bida, C., and Hancianu, M. (2025). Salivary biomarkers as a predictive factor in anxiety, depression, and stress. Curr. Issues Mol. Biol., 47.","DOI":"10.3390\/cimb47070488"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Visentin, D., Gobin, I., and Maglica, \u017d. (2023). Periodontal pathogens and their links to neuroinflammation and neurodegeneration. Microorganisms, 11.","DOI":"10.3390\/microorganisms11071832"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1902\/jop.2010.100319","article-title":"Salivary stress markers, stress, and periodontitis: A pilot study","volume":"82","author":"Rai","year":"2011","journal-title":"J. Periodontol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1111\/j.1600-0757.2009.00311.x","article-title":"Subgingival biofilm formation","volume":"52","author":"Kuboniwa","year":"2010","journal-title":"Periodontol. 2000"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1902\/jop.2009.080334","article-title":"Stress, depression, cortisol, and periodontal disease","volume":"80","author":"Rosania","year":"2009","journal-title":"J. Periodontol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1016\/S1471-4906(03)00173-X","article-title":"How stress influences the immune response","volume":"24","author":"Padgett","year":"2003","journal-title":"Trends Immunol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1111\/jcpe.13420","article-title":"Periodontal diseases and depression: A pre-clinical in vivo study","volume":"48","author":"Virto","year":"2021","journal-title":"J. Clin. Periodontol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"100450","DOI":"10.1016\/j.bbih.2022.100450","article-title":"Biomarkers common for inflammatory periodontal disease and depression: A systematic review","volume":"21","author":"Neupane","year":"2022","journal-title":"Brain Behav. Immun. Health"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"65","DOI":"10.2174\/1874210601509010065","article-title":"Maternal deprivation of Lewis rat pups increases the severity of experimental periodontitis in adulthood","volume":"9","author":"Breivik","year":"2015","journal-title":"Open Dent. J."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2685","DOI":"10.1007\/s00784-018-2611-y","article-title":"Is depression associated with oral health outcomes in adults and elders? A systematic review and meta-analysis","volume":"22","author":"Cademartori","year":"2018","journal-title":"Clin. Oral Investig."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Sioustis, I.A., Martu, M.A., Aminov, L., Pavel, M., Cianga, P., Kappenberg-Nitescu, D.C., Luchian, I., Solomon, S.M., and Martu, S. (2021). Salivary metalloproteinase-8 and metalloproteinase-9 evaluation in patients undergoing fixed orthodontic treatment before and after periodontal therapy. Int. J. Environ. Res. Public Health, 18.","DOI":"10.3390\/ijerph18041583"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/j.molmed.2014.11.004","article-title":"Polymicrobial synergy and dysbiosis in inflammatory disease","volume":"21","author":"Lamont","year":"2015","journal-title":"Trends Mol. Med."}],"container-title":["Journal of Clinical Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2077-0383\/15\/12\/4402\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T10:49:07Z","timestamp":1781002147000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2077-0383\/15\/12\/4402"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,6]]},"references-count":41,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["jcm15124402"],"URL":"https:\/\/doi.org\/10.3390\/jcm15124402","relation":{},"ISSN":["2077-0383"],"issn-type":[{"value":"2077-0383","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,6]]}}}