{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T14:33:47Z","timestamp":1793370827955,"version":"4.5.0"},"reference-count":27,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T00:00:00Z","timestamp":1780704000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Background\/Objectives: Chorioamnionitis (CA), an inflammation, with or without infection, involving the amniotic fluid, placenta, fetal membranes or decidua, can significantly impact fetal and neonatal development. This study aimed to determine the prevalence of chorioamnionitis and confirm its correlation with neonatal morbidity and mortality, in a single tertiary center. Methods: This observational, retrospective study was conducted over three years (2019\u20132021) in a tertiary neonatal intensive care unit, examining 80 preterm infants born at 23\u201328 weeks of gestation. Spearman rank correlation, \u03c72 tests, and multivariate logistic regression were used to assess associations between chorioamnionitis exposure and neonatal outcomes. Results: Among the 80 newborns analysed, clinical chorioamnionitis was identified in 12 preterm infants, while 65 (81.3%) presented histological chorioamnionitis. No significant association was found between histological chorioamnionitis stage and gestational age at birth (Spearman \u03c1 = \u22120.15, p = 0.195). Premature rupture of membranes was significantly more frequent in the CA-exposed group (46.2% vs. 13.3%, p = 0.019). In unadjusted analyses, histological chorioamnionitis exposure was associated with higher rates of adverse neonatal outcomes, including early-onset sepsis (46.2% vs. 26.7%), intraventricular haemorrhage (73.8% vs. 60.0%), bronchopulmonary dysplasia (15.9% vs. 6.7%), and retinopathy of prematurity (11.3% vs. 0.0%); however, most of these differences did not reach statistical significance. After multivariate adjustment, histological chorioamnionitis remained independently associated with severe respiratory distress syndrome (adjusted OR 25.84, 95% CI 2.49\u2013268.44, p = 0.006). Mortality was numerically lower in the CA-exposed group (27.7% vs. 46.7%); however, this difference did not reach statistical significance (p = 0.216). Conclusions: Histological chorioamnionitis was independently associated with severe respiratory distress syndrome. Associations with early onset sepsis, bronchopulmonary dysplasia, and retinopathy of prematurity were observed in unadjusted analyses but were not independently significant after adjustment for perinatal confounders. No significant association was found between chorioamnionitis and neonatal mortality. While clinical diagnostic criteria for chorioamnionitis demonstrated good specificity, their poor sensitivity underscores the urgent need for improved diagnostic tools, including routine histological examination of the placenta.<\/jats:p>","DOI":"10.3390\/jcm15124406","type":"journal-article","created":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T05:43:46Z","timestamp":1780897426000},"page":"4406","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Chorioamnionitis and Neonatal Morbidity and Mortality in Extremely Preterm Infants Born at 23\u201328 Weeks: A Single-Centre Retrospective Study"],"prefix":"10.3390","volume":"15","author":[{"given":"Gabriela C.","family":"Zaharie","sequence":"first","affiliation":[{"name":"Neonatology Department, \u201cIuliu Ha\u0163ieganu\u201d University of Medicine and Pharmacy, 400006 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9143-5664","authenticated-orcid":false,"given":"Monica G.","family":"H\u0103\u015fm\u0103\u015fanu","sequence":"additional","affiliation":[{"name":"Neonatology Department, \u201cIuliu Ha\u0163ieganu\u201d University of Medicine and Pharmacy, 400006 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernestine","family":"Haralambous","sequence":"additional","affiliation":[{"name":"Pediatrics Department, Centre Hospitalier du Valais Romand, 1951 Sion, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flaviu A.","family":"Zaharie","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Vienna General Hospital, Medical University of Vienna, 1090 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna D.","family":"Jakab","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, \u201cIuliu Ha\u021bieganu\u201d University of Medicine and Pharmacy, 400006 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0677-5846","authenticated-orcid":false,"given":"Melinda","family":"Matyas","sequence":"additional","affiliation":[{"name":"Neonatology Department, \u201cIuliu Ha\u0163ieganu\u201d University of Medicine and Pharmacy, 400006 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1097\/AOG.0000000000001246","article-title":"Evaluation and Management of Women and Newborns with a Maternal Diagnosis of Chorioamnionitis: Summary of a Workshop","volume":"127","author":"Higgins","year":"2016","journal-title":"Obstet. Gynecol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1016\/j.pedneo.2017.09.001","article-title":"Intrauterine inflammation, infection, or both (Triple I): A new concept for chorioamnionitis","volume":"59","author":"Peng","year":"2018","journal-title":"Pediatr. Neonatol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"101146","DOI":"10.1016\/j.siny.2020.101146","article-title":"The fetal inflammatory response syndrome: The origins of a concept, pathophysiology, diagnosis, and obstetrical implications","volume":"25","author":"Jung","year":"2020","journal-title":"Semin. Fetal Neonatal Med."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Giovannini, E., Bonasoni, M.P., Pascali, J.P., Giorgetti, A., Pelletti, G., Gargano, G., Pelotti, S., and Fais, P. (2023). Infection Induced Fetal Inflammatory Response Syndrome (FIRS): State-of-the-Art and Medico-Legal Implications\u2014A Narrative Review. Microorganisms, 11.","DOI":"10.3390\/microorganisms11041010"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"S807","DOI":"10.1016\/j.ajog.2023.02.002","article-title":"Clinical chorioamnionitis at term: Definition, pathogenesis, microbiology, diagnosis, and treatment","volume":"230","author":"Jung","year":"2024","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"S29","DOI":"10.1016\/j.ajog.2015.08.040","article-title":"Acute chorioamnionitis and funisitis: Definition, pathologic features, and clinical significance","volume":"213","author":"Kim","year":"2015","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1007\/s10024-003-7070-y","article-title":"Amniotic infection syndrome: Nosology and reproducibility of placental reaction patterns","volume":"6","author":"Redline","year":"2003","journal-title":"Pediatr. Dev. Pathol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"698","DOI":"10.5858\/arpa.2015-0225-CC","article-title":"Sampling and Definitions of Placental Lesions: Amsterdam Placental Workshop Group Consensus Statement","volume":"140","author":"Khong","year":"2016","journal-title":"Arch. Pathol. Lab. Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"101112","DOI":"10.1016\/j.siny.2020.101112","article-title":"Therapeutic interventions for fetal inflammatory response syndrome (FIRS)","volume":"25","author":"Xiong","year":"2020","journal-title":"Semin. Fetal Neonatal Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.jri.2016.11.008","article-title":"Preterm birth: Inflammation, fetal injury and treatment strategies","volume":"119","author":"Boyle","year":"2017","journal-title":"J. Reprod. Immunol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1007\/s00281-020-00803-2","article-title":"Preterm birth and sustained inflammation: Consequences for the neonate","volume":"42","author":"Humberg","year":"2020","journal-title":"Semin. Immunopathol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Olgu\u00edn-Ortega, A., Figueroa-Damian, R., Palafox-Vargas, M.L., and Reyes-Mu\u00f1oz, E. (2024). Risk of adverse perinatal outcomes among women with clinical and subclinical histopathological chorioamnionitis. Front. Med., 11.","DOI":"10.3389\/fmed.2024.1242962"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"35","DOI":"10.5582\/bst.2014.01058","article-title":"Perinatal outcomes of pregnancies complicated by preterm premature rupture of the membranes before 34 weeks of gestation","volume":"9","author":"Yu","year":"2015","journal-title":"Biosci. Trends"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1097\/AOG.0000000000004377","article-title":"Chorioamnionitis and Risk for Maternal and Neonatal Sepsis: A Systematic Review and Meta-analysis","volume":"137","author":"Beck","year":"2021","journal-title":"Obstet. Gynecol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e13834","DOI":"10.1542\/peds.2015-2323","article-title":"Chorioamnionitis and Culture-Confirmed, Early-Onset Neonatal Infections","volume":"137","author":"Wortham","year":"2016","journal-title":"Pediatrics"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e20182896","DOI":"10.1542\/peds.2018-2896","article-title":"Management of Neonates Born at \u226434 6\/7 Weeks\u2019 Gestation with Suspected or Proven Early-Onset Bacterial Sepsis","volume":"142","author":"Puopolo","year":"2018","journal-title":"Pediatrics"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Edwards, S.A., and House, M. (2024). Cerclage prevents ascending intrauterine infection in pregnant mice. Am. J. Obstet. Gynecol., 230.","DOI":"10.1016\/j.ajog.2023.10.004"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2265","DOI":"10.1080\/14767058.2017.1340447","article-title":"High- but not low-grade histologic chorioamnionitis is associated with spontaneous preterm birth in a Swedish cohort","volume":"31","author":"Fahmi","year":"2018","journal-title":"J. Matern. Fetal Neonatal Med."},{"key":"ref_19","first-page":"1939","article-title":"Association of histological and clinical chorioamnionitis with perinatal and neonatal outcome","volume":"32","author":"Han","year":"2019","journal-title":"J. Matern. Fetal Neonatal Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1007\/s00431-025-06146-0","article-title":"The predictive value of maternal and neonatal inflammatory biomarkers for necrotizing enterocolitis","volume":"184","author":"Matyas","year":"2025","journal-title":"Eur. J. Pediatr."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e1914611","DOI":"10.1001\/jamanetworkopen.2019.14611","article-title":"Association of Chorioamnionitis with Bronchopulmonary Dysplasia among Preterm Infants: A systematic review and meta-analysis","volume":"2","author":"Ghazi","year":"2019","journal-title":"JAMA Netw. Open"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1124","DOI":"10.1542\/peds.2008-0862","article-title":"Patterns of respiratory disease during the first 2 postnatal weeks in extremely premature infants","volume":"123","author":"Laughon","year":"2009","journal-title":"Pediatrics"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"701","DOI":"10.2298\/SARH1512701H","article-title":"Parental Factors Associated with Intrauterine Growth Restriction","volume":"143","author":"Hasmasanu","year":"2015","journal-title":"Srp. Arh. Celok. Lek."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1040","DOI":"10.1002\/ijgo.15290","article-title":"Effect of acute histologic chorioamnionitis on bronchopulmonary dysplasia and mortality rate among extremely low gestational age neonates","volume":"165","author":"Costa","year":"2024","journal-title":"Int. J. Gynaecol. Obstet."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1038\/s41390-021-01633-0","article-title":"Chorioamnionitis and neonatal outcomes","volume":"91","author":"Jain","year":"2022","journal-title":"Pediatr. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1055\/s-0033-1361823","article-title":"Fetal immune response to chorioamnionitis","volume":"32","author":"Kallapur","year":"2014","journal-title":"Semin. Reprod. Med."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"23","DOI":"10.11138\/jpm\/2016.10.3.023","article-title":"Predictive value of procalcitonin or C-reactive protein for subclinical intrauterine infection in patients with PROM","volume":"10","author":"Li","year":"2016","journal-title":"J. Prenat. Med."}],"container-title":["Journal of Clinical Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2077-0383\/15\/12\/4406\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T06:20:54Z","timestamp":1780899654000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2077-0383\/15\/12\/4406"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,6]]},"references-count":27,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["jcm15124406"],"URL":"https:\/\/doi.org\/10.3390\/jcm15124406","relation":{},"ISSN":["2077-0383"],"issn-type":[{"value":"2077-0383","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,6]]}}}