{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:36:58Z","timestamp":1785429418987,"version":"3.56.0"},"reference-count":24,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T00:00:00Z","timestamp":1783296000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005888","name":"Medical University of Lodz","doi-asserted-by":"crossref","award":["503\/5-06102\/503-51-001-18"],"award-info":[{"award-number":["503\/5-06102\/503-51-001-18"]}],"id":[{"id":"10.13039\/501100005888","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100005888","name":"Medical University of Lodz","doi-asserted-by":"crossref","award":["503\/5-061-02\/503-51-001-17"],"award-info":[{"award-number":["503\/5-061-02\/503-51-001-17"]}],"id":[{"id":"10.13039\/501100005888","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100005888","name":"Medical University of Lodz","doi-asserted-by":"crossref","award":["503\/5-061-02\/503-51-002-18"],"award-info":[{"award-number":["503\/5-061-02\/503-51-002-18"]}],"id":[{"id":"10.13039\/501100005888","id-type":"DOI","asserted-by":"crossref"}]},{"award":["503\/5-06102\/503-51-001-18"],"award-info":[{"award-number":["503\/5-06102\/503-51-001-18"]}],"id":[{"id":"https:\/\/ror.org\/02t4ekc95","id-type":"ROR","asserted-by":"publisher"}]},{"award":["503\/5-061-02\/503-51-001-17"],"award-info":[{"award-number":["503\/5-061-02\/503-51-001-17"]}],"id":[{"id":"https:\/\/ror.org\/02t4ekc95","id-type":"ROR","asserted-by":"publisher"}]},{"award":["503\/5-061-02\/503-51-002-18"],"award-info":[{"award-number":["503\/5-061-02\/503-51-002-18"]}],"id":[{"id":"https:\/\/ror.org\/02t4ekc95","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JCM"],"abstract":"<jats:p>Background\/Objectives: Oral squamous cell carcinoma (OSCC) remains clinically heterogeneous, and prognosis is not always fully explained by conventional clinicopathological parameters. Systemic inflammation and tumor metabolic alterations may provide complementary information on tumor biology. This study aimed to assess associations between preoperative inflammatory markers, isotope ratio mass spectrometry (IRMS)-derived tumor signatures, clinicopathological features, and survival outcomes in OSCC. Methods: This exploratory retrospective secondary analysis included 50 consecutive patients with surgically treated, histologically confirmed OSCC. Preoperative blood-based markers, including neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), systemic immune-inflammation index (SII), white blood cell count, lymphocyte count, and C-reactive protein, were retrieved from routine laboratory tests. Matched tumor, surgical margin, and healthy oral mucosa samples were analyzed by IRMS for \u03b413C, \u03b415N, carbon and nitrogen content, and [N]\/[C] ratio. Associations with clinicopathological variables, nodal status, overall survival (OS), and disease-free survival (DFS) were evaluated using non-parametric tests, Spearman correlations, and Cox regression models. Results: Tumor tissue showed a consistent isotope and elemental phenotype compared with healthy mucosa, including higher nitrogen content, lower carbon content, increased [N]\/[C] ratio, lower \u03b415N, and less negative \u03b413C values. NLR, PLR, SII, and CRP were not robustly associated with standard clinicopathological features after correction for multiple testing. Correlations between inflammatory and isotope-derived parameters were modest. Higher NLR was associated with worse OS and DFS and remained significant after adjustment for pathologic nodal status. Less negative tumor \u03b413C showed a potential adverse prognostic signal. Conclusions: Systemic inflammatory markers and IRMS-derived tumor signatures appear to reflect partly distinct biological domains in OSCC. NLR may provide accessible prognostic information, while tumor \u03b413C warrants further validation as a metabolic biomarker.<\/jats:p>","DOI":"10.3390\/jcm15135278","type":"journal-article","created":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T13:43:14Z","timestamp":1783345394000},"page":"5278","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Systemic Inflammation, Tumor Isotopic Signatures, and Prognosis in Oral Squamous Cell Carcinoma: Exploratory Integration of Blood- and Tissue-Derived Biomarkers\u2014An Exploratory Retrospective Secondary Analysis"],"prefix":"10.3390","volume":"15","author":[{"given":"Katarzyna","family":"Bogusiak","sequence":"first","affiliation":[{"name":"Department of Maxillofacial Surgery, Medical University of Lodz, 251 Pomorska, 92-209 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3091-8387","authenticated-orcid":false,"given":"Piotr","family":"Paneth","sequence":"additional","affiliation":[{"name":"Institute of Applied Radiation Chemistry, Lodz University of Technology, 116 \u017beromskiego, 90-924 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcin","family":"Majchrzak","sequence":"additional","affiliation":[{"name":"Department of Maxillofacial Surgery, Medical University of Lodz, 251 Pomorska, 92-209 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9200-2828","authenticated-orcid":false,"given":"Marcin","family":"Kozakiewicz","sequence":"additional","affiliation":[{"name":"Department of Maxillofacial Surgery, Medical University of Lodz, 251 Pomorska, 92-209 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7197-3334","authenticated-orcid":false,"given":"J\u00f3zef","family":"Kobos","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Medical University of Lodz, 251 Pomorska, 92-209 Lodz, Poland"},{"name":"Department of Pathology, Medical University of Lodz, 251 Pomorska, 92-209 Lodz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"100988","DOI":"10.1016\/j.disamonth.2020.100988","article-title":"Epidemiologic aspects of oral cancer","volume":"66","author":"Sarode","year":"2020","journal-title":"Dis.-A-Mon."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1158\/2159-8290.CD-21-1059","article-title":"Hallmarks of Cancer: New Dimensions","volume":"12","author":"Hanahan","year":"2022","journal-title":"Cancer Discov."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1111\/jop.12688","article-title":"Meta-analysis of the prognostic value of the neutrophil-to-lymphocyte ratio in oral squamous cell carcinoma","volume":"47","author":"Wang","year":"2018","journal-title":"J. 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