{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T16:06:51Z","timestamp":1784650011347,"version":"3.55.0"},"reference-count":20,"publisher":"MDPI AG","issue":"14","license":[{"start":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:00:00Z","timestamp":1784592000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Clin. Med.","JCM"],"abstract":"<jats:p>Purpose: The aim of this study was to test the hypothesis that Tenascin-C (TNC) levels increase following thoracic radiotherapy (RT), and that elevated serum TNC levels are associated with corresponding increases in myocardial TNC expression in histopathological specimens, supporting its role as a potential biomarker of radiation-induced cardiotoxicity. Methods: Blood and cardiac tissue samples from rats 8 weeks after thoracic radiotherapy were analyzed to evaluate changes in serum TNC levels and myocardial TNC expression, and their association with radiation exposure. Male Wistar rats were randomized to a single 12-Gy dose of mediastinal irradiation or sham irradiation. Serum TNC levels were assessed with rat ELISA kits. Myocardial TNC expression was assessed using immunohistochemistry, and staining intensity was semi-quantitatively graded by blinded observers. Results: Serum Tenascin-C (TNC) levels were elevated in RT only group compared to control (p &lt; 0.001), paralleled by a marked increase in myocardial TNC expression (p &lt; 0.001). A consistent concordance between circulating and tissue-level TNC was observed, supporting its potential as a biomarker of radiation-induced cardiac injury. Conclusions: Significant associations were observed between radiation exposure and both serum Tenascin-C levels and myocardial Tenascin-C expression, demonstrating a concordance between circulating and tissue findings and supporting Tenascin-C as a promising biomarker of radiation-induced cardiotoxicity.<\/jats:p>","DOI":"10.3390\/jcm15145724","type":"journal-article","created":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T15:17:52Z","timestamp":1784647072000},"page":"5724","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Blood Biomarker Tenascin-C for Detection of Radiation Cardiotoxicity: An Animal Model"],"prefix":"10.3390","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8073-3487","authenticated-orcid":false,"given":"Ecem","family":"Demir","sequence":"first","affiliation":[{"name":"Department of Radiation Oncology Istanbul, Ba\u015fak\u015fehir \u00c7am and Sakura City Hospital, Istanbul 34480, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6571-415X","authenticated-orcid":false,"given":"Karolin","family":"Yanar","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Cerrahpa\u015fa Faculty of Medicine, Istanbul University-Cerrahpa\u015fa, Istanbul 34320, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P\u0131nar","family":"Atukeren","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Cerrahpa\u015fa Faculty of Medicine, Istanbul University-Cerrahpa\u015fa, Istanbul 34320, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serbay","family":"Ozkan","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine, Izmir Katipcelebi University, Izmir 35620, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00f6zde","family":"Erkanl\u0131 \u015eent\u00fcrk","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Cerrahpa\u015fa Faculty of Medicine, Istanbul University-Cerrahpa\u015fa, Istanbul 34098, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1739-8329","authenticated-orcid":false,"given":"Melike","family":"\u00dclker","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Avrasya Hospital, Istanbul 34020, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u015eefika Arzu","family":"Ergen","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology Istanbul, Cerrahpa\u015fa Faculty of Medicine, Istanbul University-Cerrahpa\u015fa, Istanbul 34098, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0904-489X","authenticated-orcid":false,"given":"Song\u00fcl","family":"Kara\u00e7am","sequence":"additional","affiliation":[{"name":"Radiotherapy Program, Vocational School of Health Services, Istanbul University-Cerrahpa\u015fa, Istanbul 34098, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fazilet","family":"\u00d6ner Din\u00e7ba\u015f","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology Istanbul, Cerrahpa\u015fa Faculty of Medicine, Istanbul University-Cerrahpa\u015fa, Istanbul 34098, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/j.jtho.2016.09.134","article-title":"Heart Dose Is an Independent Dosimetric Predictor of Overall Survival in Locally Advanced Non-Small Cell Lung Cancer","volume":"12","author":"Speirs","year":"2017","journal-title":"J. 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